| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,403,448 | G→A | 52.6% | A135V (GCG→GTG) | dapF ← | diaminopimelate epimerase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,403,448 | 0 | G | A | 52.6% | 3.0 / 86.1 | 97 | A135V (GCG→GTG) | dapF | diaminopimelate epimerase |
| Reads supporting (aligned to +/- strand): ref base G (27/19); new base A (33/18); total (60/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.76e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.75e-01 | |||||||||||
Only 100 of 101 total aligned reads displayed.
TTCCCATCGACACCACGCCGCATAAGATTGTCTGCTCGGCGGCGCGCATAATATAGGTCTTTTCCGCTTTGTTAGCGCGAAACGGCACGGCGGAAGGTTCGAAGTTGGGTTCGCCCATATTTACGCGGACCAG > minE/2403383‑2403515 | ttCCCATCGACACCACGCCACATAAGATTGTCTGCTCGGCGGCGCGCATGATATAGGTCTTTTCCACtt > 1:2159124/1‑69 (MQ=255)ttCCCATCGACACCACGCCACATAAGATTGTCTGCTCGGCGGCGCGCATGATATAGGTCTTTTCCACtt > 1:1000210/1‑69 (MQ=255)ttCCCATCGACACCACGCCACATAAGATTGTCTGCTCGGCGGCGCGCATGATATAGGTCTTTTCCACtt > 1:2440756/1‑69 (MQ=255)ttCCCATCGACACCACGCCACATAAGATTGTCTGCTCGGCGGCGCGCATGATATAGGTCTTTTCCACtt > 1:1954590/1‑69 (MQ=255)ttCCCATCGACACCACGCCACATAAGATTGTCTGCTCGGCGGCGCGCATGATATAGGTCTTTTCCACtt > 1:2666315/1‑69 (MQ=255)ttCCCATCGACACCACGCCACATAAGATTGTCTGCTCGGCGGCGCGCATGATATAGGTCTTTTCCACtt > 1:2823932/1‑69 (MQ=255)ttCCCATCGACACCACGCCACATAAGATTGTCTGCTCGGCGGCGCGCATGATATAGGTCTTTTCCACtt > 1:1517806/1‑69 (MQ=255)ttCCCATCGACACCACGCCACATAAGATTGTCTGCTCGGCGGCGCGCATGATATAGGTCTTTTCCACtt > 1:2108569/1‑69 (MQ=255)ttCCCATCGACACCACGCCACATAAGATTGTCTGCTCGGCGGCGCGCATGATATAGGTCTTTTCCACtt > 1:1345605/1‑69 (MQ=255)ttCCCATCGACACCACGCCACATAAGATTGTCTGCTCGGCGGCGCGCATGATATAGGTCTTTTCCACtt > 1:122122/1‑69 (MQ=255)ttCCCATCGACACCACGCCACATAAGATTGTCTGCTCGGCGGCGCGCATGATATAGGTCTTTTCCACtt > 1:2175336/1‑69 (MQ=255)ttCCCATCGACACCACGCCACATAAGATTGTCTGCTCGGCGGCGCGCATGATATAGGTCTTTTCCACtt > 1:3347166/1‑69 (MQ=255)ttCCCATCGACACCACGCCACATAAGATTGTCTGCTCGGCGGCGCGCATGATATAGGTCTTTTCCACtt > 1:355092/1‑69 (MQ=255)ttCCCATCGACACCACGCCACATAAGATTCTCTGCTCGGCGGCGCGCATGATATAGGTCTTTTCCACtt > 1:626887/1‑69 (MQ=255) aTCGACACCACGCCACATAAGATTGTCTGCTCGGCGGCGCGCATGATATAGGTCTTTTCCACTTTGtt > 1:1331406/1‑68 (MQ=255) aTCGACACCACGCCACATAAGATTGTCTGCTCGGCGGCGCGCATGATATAGGTCTTTTCCACTTTGtt > 1:1337554/1‑68 (MQ=255) aTCGACACCACGCCACATAAGATTGTCTGCTCGGCGGCGCGCATGATATAGGTCTTTTCCACTTTGTTa > 1:3127892/1‑69 (MQ=255) aTCGACACCACGCCACATAAGATTGTCTGCTCGGCGGCGCGCATGATATAGGTCTTTTCCACTTTGTTa > 1:2596683/1‑69 (MQ=255) aTCGACACCACGCCACATAAGATTGTCTGCTCGGCGGCGCGCATGATATAGGTCTTTTCCACTTTGTTa > 1:2599015/1‑69 (MQ=255) aTCGACACCACGCCACATAAGATTGTCTGCTCGGCGGCGCGCATGATATAGGTCTTTTCCACTTTGTTa > 1:2747737/1‑69 (MQ=255) aTCGACACCACGCCACATAAGATTGTCTGCTCGGCGGCGCGCATGATATAGGTCTTTTCCACTTTGTTa > 1:2830041/1‑69 (MQ=255) aTCGACACCACGCCACATAAGATTGTCTGCTCGGCGGCGCGCATGATATAGGTCTTTTCCACTTTGTTa > 1:2995312/1‑69 (MQ=255) aTCGACACCACGCCACATAAGATTGTCTGCTCGGCGGCGCGCATGATATAGGTCTTTTCCACTTTGTTa > 1:2546491/1‑69 (MQ=255) aTCGACACCACGCCACATAAGATTGTCTGCTCGGCGGCGCGCATGATATAGGTCTTTTCCACTTTGTTa > 1:2516793/1‑69 (MQ=255) aTCGACACCACGCCACATAAGATTGTCTGCTCGGCGGCGCGCATGATATAGGTCTTTTCCACTTTGTTa > 1:3159866/1‑69 (MQ=255) aTCGACACCACGCCACATAAGATTGTCTGCTCGGCGGCGCGCATGATATAGGTCTTTTCCACTTTGTTa > 1:791706/1‑69 (MQ=255) aTCGACACCACGCCACATAAGATTGTCTGCTCGGCGGCGCGCATGATATAGGTCTTTTCCACTTTGTTa > 1:2413749/1‑69 (MQ=255) aTCGACACCACGCCACATAAGATTGTCTGCTCGGCGGCGCGCATGATATAGGTCTTTTCCACTTTGTTa > 1:3218376/1‑69 (MQ=255) aTCGACACCACGCCACATAAGATTGTCTGCTCGGCGGCGCGCATGATATAGGTCTTTTCCACTTTGTTa > 1:1200624/1‑69 (MQ=255) aTCGACACCACGCCACATAAGATTGTCTGCTCGGCGGCGCGCATGATATAGGTCTTTTACACTTTGTTa > 1:791870/1‑69 (MQ=255) aTCGACACCACGCCACATAAGATTGTCTGCTAGGCGGCGCGCATGATATAGGTCTTTTCCACTTTGTTa > 1:790327/1‑69 (MQ=255) aTCGACACCACGACACATAAGATTGTCTGCTCGGCGGCGCGCATGATATAGGTCTTTTCCACTTTg > 1:3169480/1‑66 (MQ=255) gACACCACGCCGCATAAGATTGTCTGCTCGGCGGCGCGCATAATATAGGTCTTTTCCGCTTTGTTAgc > 1:3020558/1‑68 (MQ=255) gACACCACGCCGCATAAGATTGTCTGCTCGGCGGCGCGCATAATATAGGTCTTTTCCGCTTTGTTAgc > 1:345720/1‑68 (MQ=255) cacGCCGCATAAGATTGTCTGCTCGGCGGCGCGCATAATATAGGTCTTTTCCGCTTTGTTAGCGCGaaa > 1:1130452/1‑69 (MQ=255) gccgcATAAGATTGTCTGCTCGGCGGCGCGCATAATATAGGTCTTTTCCGCTTTGTTAGCGCGAAacgg < 1:3054866/69‑1 (MQ=255) gccgcATAAGATTGTCTGCTCGGCGGCGCGCATAATATAGGTCTTTTCCGCTTTGTTAGCGCGAAacgg < 1:1451380/69‑1 (MQ=255) aGATTGTCTGCTCGGCGGCGCGCATAATATAGGTCTTTTCCGCTTTGTTAGCGCGAAacggcacggc > 1:2590816/1‑67 (MQ=255) aGATTGTCTGCTCGGCGGCGCGCATAATATAGGTCTTTTCCGCTTTGTTAGCGCGAAacggcacggc > 1:257105/1‑67 (MQ=255) aGATTGTCTGCTCGGCGGCGCGCATAATATAGGTCTTTTCCGCTTTGTTAGCGCGAAacggcacggc > 1:2234258/1‑67 (MQ=255) ttGTCTGCTCGGCGGCGCGCATAATATAGGTCTTTTCCGCTTTGTTAGCGCGAAACGGCACGGCGGAAg < 1:3072584/69‑1 (MQ=255) ttGTCTGCTCGGCGGCGCGCATAATATAGGTCTTTTCCGCTTTGTTAGCGCGAAACGGCACGGCGGAAg < 1:2323888/69‑1 (MQ=255) ttGTCTGCTCGGCGGCGCGCATAATATAGGTCTTTTCCGCTTTGTTAGCGCGAAACGGCACGGCGGAAg < 1:3191070/69‑1 (MQ=255) ttGTCTGCTCGGCGGCGCGCATAATATAGGTCTTTTCCGCTTTGTTAGCGCGAAACGGCACGGCGGAAg < 1:336513/69‑1 (MQ=255) ttGTCTGCTCGGCGGCGCGCATAATATAGGTCTTTTCCGCTTTGTTAGCGCGAAACGGCACGGCGGAAg < 1:915114/69‑1 (MQ=255) ttGTCTGCTCGGCGGCGCGCATAATATAGGTCTTTTCCGCTTTGTTAGCGCGAAACGGCACGGCGGAAg < 1:1060580/69‑1 (MQ=255) ttGTCTGCTCGGCGGCGCGCATAATATAGGTCTTTTCCGCTTTGTTAGCGCGAAACGGCACGGCGGAAg < 1:1459513/69‑1 (MQ=255) ttGTCTGCTCGGCGGCGCGCATAATATAGGTCTTTTCCGCTTTGTTAGCGCGAAACGGCACGGCGGAAg < 1:1105262/69‑1 (MQ=255) tGCTCGGCGGCTCGCATGATATAGGTCTTTTCCACTTTGTTa < 1:729958/42‑1 (MQ=255) tGCTCGGCGGCTCGCATGATATAGGTCTTTTCCACTTTGTTa < 1:202777/42‑1 (MQ=255) tGCTCGGCGGCGCGCTTGATATAGGTCTTTTCCACTTTGTTa < 1:2860530/42‑1 (MQ=255) tGCTCGGCGGCGCGCATGATATAGGTCTTTTCCACTTTGTTa < 1:3283970/42‑1 (MQ=38) tGCTCGGCGGCGCGCATGATATAGGTCTTTTCCACTTTGTTa < 1:1640199/42‑1 (MQ=38) tGCTCGGCGGCGCGCATGATATAGGTCTTTTCCACTTTGTTa < 1:1189465/42‑1 (MQ=38) tGCTCGGCGGCGCGCATGATATAGGTCTTTTCCACTTTGTTa < 1:1152570/42‑1 (MQ=38) tGCTCGGCGGCGCGCATGATATAGGTCTTTTCCACTTTGTTa < 1:1562143/42‑1 (MQ=38) tGCTCGGCGGCGCGCATGATATAGGTCTTTTCCACTTTGTTa < 1:1275393/42‑1 (MQ=38) tGCTCGGCGGCGCGCATGATATAGGTCTTTTCCACTTTGTTa < 1:1812577/42‑1 (MQ=38) tGCTCGGCGGCGCGCATGATATAGGTCTTTTCCACTTTGTTa < 1:1903481/42‑1 (MQ=38) tGCTCGGCGGCGCGCATGATATAGGTCTTTTCCACTTTGTTa < 1:1953314/42‑1 (MQ=38) tGCTCGGCGGCGCGCATGATATAGGTCTTTTCCACTTTGTTa < 1:2025570/42‑1 (MQ=38) tGCTCGGCGGCGCGCATGATATAGGTCTTTTCCACTTTGTTa < 1:585684/42‑1 (MQ=38) tGCTCGGCGGCGCGCATGATATAGGTCTTTTCCACTTTGTTa < 1:2075113/42‑1 (MQ=38) tGCTCGGCGGCGCGCATGATATAGGTCTTTTCCACTTTGTTa < 1:1075029/42‑1 (MQ=38) gCTCGGCGGCGCGCATGATATAGGTCTTTTCCACTTTGTTa < 1:1901532/41‑1 (MQ=38) cTCGGCGGCGCGCATGATATAGGTCTTTTCCACTTTGTTa < 1:3274241/40‑1 (MQ=38) cTCGGCGGCGCGCATAATATAGGTCTTTTCCGCTTTGTTAGCGCGAAACGGCACGGCGGAAGGTTCGaa > 1:1791726/1‑69 (MQ=255) cTCGGCGGCGCGCATAATATAGGTCTTTTCCGCTTTGTTAGCGCGAAACGGCACGGCGGAAGGTTCGaa > 1:3421459/1‑69 (MQ=255) cTCGGCGGCGCGCATAATATAGGTCTTTTCCGCTTTGTTAGCGCGAAACGGCACGGCGGAAGGTTCGaa > 1:396122/1‑69 (MQ=255) cATAATATAGGTCTTTTCCGCTTTGTTAGCGCGAAACGGCACGGCGGAAGGTTCGAAGTTGGGTTCGcc < 1:1752164/69‑1 (MQ=255) cATAATATAGGTCTTTTCCGCTTTGTTAGCGCGAAACGGCACGGCGGAAGGTTCGAAGTTGGGTTCGcc < 1:3268897/69‑1 (MQ=255) cATAATATAGGTCTTTTCCGCTTTGTTAGCGCGAAACGGCACGGCGGAAGGTTCGAAGTTGGGTTCGcc < 1:3181556/69‑1 (MQ=255) tataGGTCTTTTCCGCTTTGTTAGCGCGAAACGGCACGGCGGAAGGTTCGAAGTTGGGTTCGCCCata > 1:2168000/1‑68 (MQ=255) tataGGTCTTTTCCGCTTTGTTAGCGCGAAACGGCACGGCGGAAGGTTCGAAGTTGGGTTCGCCCata > 1:3173202/1‑68 (MQ=255) tataGGTCTTTTCCGCTTTGTTAGCGCGAAACGGCACGGCGGAAGGTTCGAAGTTGGGTTCGCCCata > 1:2164129/1‑68 (MQ=255) tataGGTCTTTTCCGCTTTGTTAGCGCGAAACGGCACGGCGGAAGGTTCGAAGTTGGGTTCGCCCata > 1:2516388/1‑68 (MQ=255) tCTTTTCCGCTTTGTTAGCGCGAAACGGCACGGCGGAAGGTTCGAAGTTGGGTTCGCCCATATTTAcg > 1:1481972/1‑68 (MQ=255) tCTTTTCCGCTTTGTTAGCGCGAAACGGCACGGCGGAAGGTTCGAAGTTGGGTTCGCCCATATTTAcg > 1:2939712/1‑68 (MQ=255) ttttCCGCTTTGTTAGCGCGAAACGGCACGGCGGAAGGTTCGAAGTTGGGTTCGCCCATATTTACGCgg > 1:1007983/1‑69 (MQ=255) ttttCCGCTTTGTTAGCGCGAAACGGCACGGCGGAAGGTTCGAAGTTGGGTTCGCCCATATTTACGCgg > 1:1445259/1‑69 (MQ=255) ttttCCGCTTTGTTAGCGCGAAACGGCACGGCGGAAGGTTCGAAGTTGGGTTCGCCCATATTTACGCgg > 1:1078330/1‑69 (MQ=255) ttttCCGCTTTGTTAGCGCGAAACGGCACGGCGGAAGGTTCGAAGTTGGGTTCGCCCATATTTACGCgg > 1:1680935/1‑69 (MQ=255) ttCCGCTTTGTTAGCGCGAAACGGCACGGCGGAAGGTTCGAAGTTGGGTTCGCCCATATTTACGCgg > 1:1484303/1‑67 (MQ=255) ttCCGCTTTGTTAGCGCGAAACGGCACGGCGGAAGGTTCGAAGTTGGGTTCGCCCATATTTACGCgg > 1:2476589/1‑67 (MQ=255) ttCCGCTTTGTTAGCGCGAAACGGCACGGCGGAAGGTTCGAAGTTGGGTTCGCCCATATTTACGCgg > 1:405998/1‑67 (MQ=255) ttCCGCTTTGTTAGCGCGAAACGGCACGGCGGAAGGTTCGAAGTTGGGTTCGCCCATATTTACGCgg > 1:2323351/1‑67 (MQ=255) ttCCGCTTTGTTAGCGCGAAACGGCACGGCGGAAGGTTCGAAGTTGGGTTCGCCCATATTTACGCgg > 1:2047085/1‑67 (MQ=255) ttCCGCTTTGTTAGCGCGAAACGGCACGGCGGAAGGTTCGAAGTTGGGTTCGCCCATATTTACGCgg > 1:617605/1‑67 (MQ=255) ttCCGCTTTGTTAGCGCGAAACGGCACGGCGGAAGGTTCGAAGTTGGGTTCGCCCATATTTACGCgg > 1:1104739/1‑67 (MQ=255) ttCCGCTTTGTTAGCGCGAAACGGCACGGCGGAAGGTTCGAAGTTGGGTTCGCCCATATTTACGCgg > 1:6570/1‑67 (MQ=255) ccGCTTTGTTAGCGCGAAACGGCACGGCGGAAGGTTCGAAGTTGGGTTCGCCCATATTTACGCGGAc < 1:1756511/67‑1 (MQ=255) ccGCTTTGTTAGCGCGAAACGGCACGGCGGAAGGTTCGAAGTTGGGTTCGCCCATATTTACGCGGAc < 1:803092/67‑1 (MQ=255) ccGCTTTGTTAGCGCGAAACGGCACGGCGGAAGGTTCGAAGTTGGGTTCGCCCATATTTACGCGGAc < 1:2154288/67‑1 (MQ=255) ccGCTTTGTTAGCGCGAAACGGCACGGCGGAAGGTTCGAAGTTGGGTTCGCCCATATTTACGCGGAc < 1:1589659/67‑1 (MQ=255) ccGCTTTGTTAGCGCGAAACGGCACGGCGGAAGGTTCGAAGTTGGGTTCGCCCATATTTACGCGGAc < 1:1600244/67‑1 (MQ=255) ccGCTTTGTTAGCGCGAAACGGCACGGCGGAAGGTTCGAAGTTGGGTTCGCCCATATTTACGCGGAc < 1:1781455/67‑1 (MQ=255) gCTTTGTTAGCGCGAAACGGCACGGCGGAAGGTTCGAAGTTGGGTTCGCCCATATTTACGCGGACCAg < 1:504263/68‑1 (MQ=255) gCTTTGTTAGCGCGAAACGGCACGGCGGAAGGTTCGAAGTTGGGTTCGCCCATATTTACGCGGACCAg < 1:1111092/68‑1 (MQ=255) gCTTTGTTAGCGCGAAACGGCACGGCGGAAGGTTCGAAGTTGGGTTCGCCCATATTTACGCGGACCAg < 1:3310183/68‑1 (MQ=255) gCTTTGTTAGCGCGAAACGGCACGGCGGAAGGTTCGAAGTTGGGTTCGCCCATATTTACGCGGACCAg < 1:1420688/68‑1 (MQ=255) | TTCCCATCGACACCACGCCGCATAAGATTGTCTGCTCGGCGGCGCGCATAATATAGGTCTTTTCCGCTTTGTTAGCGCGAAACGGCACGGCGGAAGGTTCGAAGTTGGGTTCGCCCATATTTACGCGGACCAG > minE/2403383‑2403515 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |