| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 200,183 | A→T | 25.6% | G88G (GGT→GGA) | metI ← | DL‑methionine transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 200,183 | 0 | A | T | 25.6% | 162.5 / 60.4 | 113 | G88G (GGT→GGA) | metI | DL‑methionine transporter subunit |
| Reads supporting (aligned to +/- strand): ref base A (45/39); new base T (13/16); total (58/55) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.19e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.44e-01 | |||||||||||
Only 100 of 118 total aligned reads displayed.
CCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATCCATACAAGCAAGATAATGAACGG > minE/200125‑200248 | ccATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGa < 1:3214049/67‑1 (MQ=255)ccATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGa < 1:454383/67‑1 (MQ=255)ccATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATg < 1:2320318/69‑1 (MQ=255)ccATACGGGCAATAAACGGTGCTGCACCAACGGTTAAAGGAACAATCGCTGCCTGCAAACCAATCGATg < 1:3380867/69‑1 (MQ=255) cATACGGGCAATAAACGGTTCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGa < 1:1844182/66‑1 (MQ=255) cGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTAcc > 1:3125384/1‑68 (MQ=255) cGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTAcc > 1:2662390/1‑68 (MQ=255) cGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTAcc > 1:2445890/1‑68 (MQ=255) ggCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTG‑‑‑GCAAACCAATCGATGTACCGACAAt < 1:3017627/69‑1 (MQ=255) aaTAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAAt < 1:1123501/69‑1 (MQ=255) aaTAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAAt < 1:712778/69‑1 (MQ=255) aaaCGGTGCTGCACTAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGa > 1:2815068/1‑54 (MQ=255) aaaCGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGa > 1:1461093/1‑54 (MQ=255) aaaCGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGa > 1:446263/1‑54 (MQ=255) aaaCGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGa > 1:345130/1‑54 (MQ=255) aaaCGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGa > 1:1145661/1‑54 (MQ=255) aaaCGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGa > 1:1347336/1‑54 (MQ=255) aaaCGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGa > 1:733147/1‑54 (MQ=255) aaCGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGt > 1:2341084/1‑56 (MQ=255) aaCGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGt > 1:2096489/1‑56 (MQ=255) aaCGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGt > 1:2609779/1‑56 (MQ=255) aaCGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGt > 1:2757215/1‑56 (MQ=255) aaCGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGt > 1:779453/1‑56 (MQ=255) aaCGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGt > 1:848012/1‑56 (MQ=255) aaCGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGt > 1:975278/1‑56 (MQ=255) aaCGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAAcg > 1:535060/1‑69 (MQ=255) aaCGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAAcg > 1:2147814/1‑69 (MQ=255) aaCGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAAcg > 1:939316/1‑69 (MQ=255) aaCGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAAcg > 1:2990593/1‑69 (MQ=255) aaCGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCCGCAAACCAATCGATGTACCGACAATAAcg > 1:3272196/1‑69 (MQ=255) aCCAACGGTTAACGGAACAATCGCTGCCTGCAATCCAATCGATGTACCGACAATAACGCGGGTAAACgg < 1:2910722/69‑1 (MQ=255) ccAACGGTTAACGGAACAATCGCTGCCTGCAATCCAATCGATGTACCGACAATAACGCGGGTAAACgg < 1:3350985/68‑1 (MQ=255) ccAACGGTTAACGGAACAATCGCTGCCTGCAATCCAATCGATGTACCGACAATAACGCGGGTAAACgg < 1:2772438/68‑1 (MQ=255) ccAACGGTTAACGGAACAATCGCTGCCTGCAATCCAATCGATGTACCGACAATAACGCGGGTAAACgg < 1:3048347/68‑1 (MQ=255) ccAACGGTTAACGGAACAATCGCTGCCTGCAATCCAATCGATGTACCGACAATAACGCGGGTAAACgg < 1:283613/68‑1 (MQ=255) ccAACGGTTAACGGAACAATCGCTGCCTGCAATCCAATCGATGTACCGACAATAACGCGGGTAAACgg < 1:31016/68‑1 (MQ=255) ccAACGGTTAACGGAACAATCGCTGCCTGCAATCCAATCGATGTACCGACAATAACGCGGGTAAACgg < 1:2513036/68‑1 (MQ=255) ccAACGGTTAACGGAACAATCGCTGCCTGCAATCCAATCGATGTACCGACAATAACGCGGGTAAACgg < 1:3016413/68‑1 (MQ=255) ccAACGGTTAACGGAACAATCGCTGCCTGCAATCCAATCGATGTACCGACAATAACGCGGGTAAACgg < 1:1042138/68‑1 (MQ=255) ccAACGGTTAACGGAACAATCGCTGCCTGCAATCCAATCGATGTACCGACAATAACGCGGGTAAACgg < 1:3259580/68‑1 (MQ=255) ccAACGGTTAACGGAACAATCGCTGCCTGCAATCCAATCGATGTACCGACAATAACGCGGGTAAACgg < 1:2193388/68‑1 (MQ=255) ccAACGGTTAACGGAACAATCGCTGCCTGCAATCCAATCGATGTACCGACAATAACGCGGGTAAACgg < 1:1113317/68‑1 (MQ=255) ccAACGGTTAACGGAACAATCGCTGCCTGCAATCCAATCGATGTACCGACAATAACGCGGGTAAACgg < 1:678401/68‑1 (MQ=255) aaCGGTTAACGGAACAATCGCTGCCTGCAATCCAATCGATGTACCGACAATAACGCGGGTAAACgg < 1:152229/66‑1 (MQ=255) aaCGGTTAACGGAACAATCGCTGCCTGCAATCCAATCGATGTACCGACAATAACGCGGGTAAACgg < 1:1983819/66‑1 (MQ=255) aaCGGTTAACGGAACAATCGCTGCCTGCAATCCAATCGATGTACCGACAATAACGCGGGTAAACGGaa > 1:59427/1‑68 (MQ=255) aaCGGTTAACGGAACAATCGCTGCCTGCAATCCAATCGATGTACCGACAATAACGCGGGTAAACGGAat > 1:2742998/1‑69 (MQ=255) aaCGGTTAACGGAACAATCGCTGCCTGCAATCCAATCGATGTACCGACAATAACGCGGGTAAACGGAat > 1:1742325/1‑69 (MQ=255) aaCGGTTAACGGAACAATCGCTGCCTGCAATCCAATCGATGTACCGACAATAACGCGGGTAAACGGAat > 1:2409258/1‑69 (MQ=255) aaCGGTTAACGGAACAATCGCTGCCTGCAATCCAATCGATGTACCGACAATAACGCGGGTAAACGGAat > 1:180123/1‑69 (MQ=255) aaCGGTTAACGGAACAATCGCTGCCTGCAATCCAATCGATGTACCGACAATAACGCGGGTAAACGGAat > 1:1567067/1‑69 (MQ=255) aaCGGTTAACGGAACAATCGCTGCCTGCAATCCAATCGATGTACCGACAATAACGCGGGTAAACGGAat > 1:1412970/1‑69 (MQ=255) aaCGGTTAACGGAACAATCGCTGCCTGCAATCCAATCGATGTACCGACAATAACGCGGGTAAACGGAat > 1:1310443/1‑69 (MQ=255) aaCGGTTAACGGAACAATCGCTGCCTGCAATCCAATCGATGTACCGACAATAACGCGGGTAAACGGAat > 1:294124/1‑69 (MQ=255) aaCGGTTAACGGAACAATCGCTGCCTGCAATCCAATCGATGTACCGACAATAACGCGGGTAAACGGAat > 1:2970909/1‑69 (MQ=255) aaCGGTTAACGGAACAATCGCTGCCTGCAATCCAATCGATGTACCGACAATAACGCGGGTAAACGGAat > 1:3213155/1‑69 (MQ=255) aaCGGATAACGGAACAATCGCTGCCTGCAATCCAATCGATGTACCGACAATAACGCGGCTAAACGGaa > 1:2566647/1‑68 (MQ=255) aCGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAAt < 1:1061729/50‑1 (MQ=255) ggTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAatca > 1:1932395/1‑68 (MQ=255) aCAATCGCTGCCTTCAAACCAATCGATGTACCGACAAt > 1:892570/1‑38 (MQ=255) aCAATCGCTGCCTGCAAACCAATCGATGTGCCGACAATAACGCGGGTAAACGGAATCATCCATAcaa > 1:468270/1‑67 (MQ=255) aCAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATCCATAcaa > 1:3203826/1‑67 (MQ=255) aCAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATCCATAcaa > 1:3333688/1‑67 (MQ=255) aCAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATCCATAcaa > 1:2234253/1‑67 (MQ=255) aCAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATCCATAca > 1:1715366/1‑66 (MQ=255) cGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATCCATACAAGCAAGAt < 1:1319605/69‑1 (MQ=255) cGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATCCATACAAGCAAGAt < 1:2804898/69‑1 (MQ=255) cGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATCCATACAAGCAAGAt < 1:3028680/69‑1 (MQ=255) cGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATCCATACAAGCAAGAt < 1:811864/69‑1 (MQ=255) cGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATCCATACAAGCAAGAt < 1:3076880/69‑1 (MQ=255) gCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATCCATACAAGCAAGATa > 1:2502479/1‑69 (MQ=255) gCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATCCATACAAGCAAGATa > 1:2111651/1‑69 (MQ=255) gCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATCCATACAAGCAAGATa > 1:190748/1‑69 (MQ=255) gCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATCCATACAAGCAAGATa > 1:3257404/1‑69 (MQ=255) gCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATCCATACAAGCAAGATa > 1:458907/1‑69 (MQ=255) gCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATCCATACAAGCAAGATa > 1:1870155/1‑69 (MQ=255) gCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATCCATACAAGCAAGATa > 1:1157682/1‑69 (MQ=255) gCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATCCATACAAGCAAGATa > 1:155563/1‑69 (MQ=255) gcctgcAAACCAATCGATGTACCGACAATAAGGCGGGTAAACGGAATCATCCATACAAGCAAGATAATg < 1:846677/69‑1 (MQ=255) gcctgcAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATCCATACAAGCAAGATAATg < 1:3324819/69‑1 (MQ=255) gcctgcAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATCCATACAAGCAAGATAATg < 1:424226/69‑1 (MQ=255) gcctgcAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATCCATACAAGCAAGATAATg < 1:419201/69‑1 (MQ=255) gcctgcAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATCCATACAAGCAAGATAATg < 1:1310115/69‑1 (MQ=255) gcctgcAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATCCATACAAGCAAGATAATg < 1:3340911/69‑1 (MQ=255) gcctgcAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATCCATACAAGCAAGATAATg < 1:2430266/69‑1 (MQ=255) gcctgcAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATCCATACAAGCAAGATAATg < 1:3194099/69‑1 (MQ=255) gcctgcAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATCCATACAAGCAAGATAATg < 1:2550180/69‑1 (MQ=255) gcctgcAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATCCATACAAGCAAGATAATg < 1:1242451/69‑1 (MQ=255) gcctgcAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATCCATACAAGCAAGATAATg < 1:2915814/69‑1 (MQ=255) cctgcAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATCCATACAAGCAAGATAATg < 1:1688350/68‑1 (MQ=255) tgcAAACCAATCGATGTACCGACAATAACGCGGGTAAACgg < 1:3406579/41‑1 (MQ=255) tgcAAACCAATCGATGTACCGACAATAACGCGGGTAAACgg < 1:1960929/41‑1 (MQ=255) tgcAAACCAATCGATGTACCGACAATAACGCGGGTAAACgg < 1:2993368/41‑1 (MQ=255) tgcAAACCAATCGATGTACCGACAATAACGCGGGTAAACgg < 1:2141722/41‑1 (MQ=255) cAAACCAATCGATGTACCGACAATAACGCGGGTAAACgg < 1:1089364/39‑1 (MQ=255) aaCCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATCCATACAAGCAAGATAATGAACgg < 1:781056/67‑1 (MQ=255) aaCCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATCCATACAAGCAAGATAATGAACgg < 1:1982049/67‑1 (MQ=255) aaCCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATCCATACAAGCAAGATAATGAACgg < 1:2612001/67‑1 (MQ=255) aaCCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATCCATACAAGCAAGATAATGAACgg < 1:2785172/67‑1 (MQ=255) aaCCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATCCATACAAGCAAGATAATGAACgg < 1:3048247/67‑1 (MQ=255) | CCATACGGGCAATAAACGGTGCTGCACCAACGGTTAACGGAACAATCGCTGCCTGCAAACCAATCGATGTACCGACAATAACGCGGGTAAACGGAATCATCCATACAAGCAAGATAATGAACGG > minE/200125‑200248 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |