| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,412,631 | G→T | 27.8% | R9L (CGT→CTT) | aslA → | acrylsulfatase‑like enzyme |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,412,631 | 0 | G | T | 27.8% | 154.1 / 55.0 | 104 | R9L (CGT→CTT) | aslA | acrylsulfatase‑like enzyme |
| Reads supporting (aligned to +/- strand): ref base G (24/51); new base T (19/10); total (43/61) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.45e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01 | |||||||||||
Only 100 of 104 total aligned reads displayed.
ATCATTTTGATGACATGAAACCGGAGCATTTTGCATGGAATTTTCGTTTTCACCCAAACGTCTTGTTGTTGCTGTCGCCGCCGCTCTTCCTCTCATGGCCAGCGCAGCAGATACCCCGTCAACTGCC > minE/2412572‑2412698 | atcatTTTGATGACATGAAACCGGAGCATTTTGCATGGAATTTTCGTTTTCACCCAAACGTCttgttgt > 1:268897/1‑69 (MQ=255)atcatTTTGACGACATGAAACCGGATCATTTTGCATGGAATTTTCGTTTTCACCCAAACTTCttgttgt > 1:1078812/1‑69 (MQ=255)atcatTTTGACGACATGAAACCGGATCATTTTGCATGGAATTTTCGTTTTCACCCAAACTTCttgttgt > 1:702036/1‑69 (MQ=255)atcatTTTGACGACATGAAACCGGATCATTTTGCATGGAATTTTCGTTTTCACCCAAACTTCttgttgt > 1:474355/1‑69 (MQ=255)atcatTTTGACGACATGAAACCGGATCATTTTGCATGGAATTTTCGTTTTCACCCAAACTTCttgttgt > 1:125414/1‑69 (MQ=255)atcatTTTGACGACATGAAACCGGATCATTTTGCATGGAATTTTCGTTTTCACCCAAACTTCttgttgt > 1:407231/1‑69 (MQ=255)atcatTTTGACGACATGAAACCGGATCATTTTGCATGGAATTTTCGTTTTCACCCAAACTTCttgttgt > 1:1273810/1‑69 (MQ=255)atcatTTTGACGACATGAAACCGGATCATTTTGCATGGAATTTTCGTTTTCACCCAAACTTCttgttgt > 1:1290853/1‑69 (MQ=255)atcatTTTGACGACATGAAACCGGATCATTTTGCATGGAATTTTCGTTTTCACCCAAACTTCttgttgt > 1:3225222/1‑69 (MQ=255)atcatTTTGACGACATGAAACCGGATCATTTTGCATGGAATTTTCGTTTTCACCCAAACTTCttgttgt > 1:2750468/1‑69 (MQ=255)atcatTTTGACGACATGAAACCGGATCATTTTGCATGGAATTTTCGTTTTCACCCAAACTTCttgttgt > 1:2676818/1‑69 (MQ=255)atcatTTTGACGACATGAAACCGGATCATTTTGCATGGAATTTTCGTTTTCACCCAAACTTCttgttgt > 1:1856823/1‑69 (MQ=255)atcatTTTGACGACATGAAACCCGATCATTTTGCATGGAATTTTCGTTTTCACCCAAACTTCttgttgt > 1:3321087/1‑69 (MQ=255) catTTTGATGACATGAAACCGGAGCATTTTGCATGGAATTTTCGTTTTCACCCAAACGTCttgttgtt < 1:2598946/68‑1 (MQ=255) catTTTGATGACATGAAACCGGAGCATTTTGCATGGAATTTTCGTTTTCACCCAAACGTCttgttgtt < 1:548301/68‑1 (MQ=255) catTTTGATGACATGAAACCGGAGCATTTTGCATGGAATTTTCGTTTTCACCCAAACGTCttgttgtt < 1:2216981/68‑1 (MQ=255) atTTTGATGACATGAAACCGGAGCATTTTGCATGGAATTTTCGTTTTCACCCAAACGTCttgttgtt > 1:2465973/1‑67 (MQ=255) atTTTGATGACATGAAACCGGAGCATTTTGCATGGAATTTTCGTTTTCACCCAAACGTCttgttgtt > 1:2187271/1‑67 (MQ=255) atTTTGATGACATGAAACCGGAGCATTTTGCATGGAATTTTCGTTTTCACCCAAACGTCttgttgtt > 1:1871497/1‑67 (MQ=255) atTTTGATGACATGAAACCGGAGCATTTTGCATGGAATTTTCGTTTTCACCCAAACGTCttgttgtt > 1:184298/1‑67 (MQ=255) atTTTGATGACATGAAACCGGAGCATTTTGCATGGAATTTTCGTTTTCACCCAAACGTCttgttgtt > 1:1730421/1‑67 (MQ=255) atTTTGATGACATGAAACCGGAGCATTTTGCATGGAATTTTCGTTTTCACCCAAACGTCttgttgtt > 1:1706868/1‑67 (MQ=255) atTTTGATGACATGAAACCGGAGCATTTTGCATGGAATTTTCGTTTTCACCCAAACGTCttgttgtt > 1:1432886/1‑67 (MQ=255) atTTTGATGACATGAAACCGGAGCATTTTGCATGGAATTTTCGTTTTCACCCAAACGTCttgttgtt > 1:855162/1‑67 (MQ=255) atTTTGATGACATGAAACCGGAGCATTTTGCATGGAATTTTCGTTTTCACCCAAACGTCttgttgtt > 1:1562884/1‑67 (MQ=255) tttGATGACATGAAACCGGAGCATTTTGCATGGAATTTTCGTTTTCACCCAAGCGTCTTGTTGTTGCt < 1:2949888/68‑1 (MQ=255) tttGATGACATGAAACCGGAGCATTTTGCATGGAATTTTCGTTTTCACCCAAACGTCTTGTTGTTGCt < 1:267874/68‑1 (MQ=255) tttGATGACATGAAACCGGAGCATTTTGCATGGAATTTTCGTTTTCACCCAAACGTCTTGTTGTTGCt < 1:2516751/68‑1 (MQ=255) tttGATGACATGAAACCGGAGCATTTTGCATGGAATTTTCGTTTTCACCCAAACGTCTTGTTGTTGCt < 1:2097736/68‑1 (MQ=255) tttGATGACATGAAACCGGAGCATTTTGCATGGAATTTTCGTTTTCACCCAAACGTCTTGTTGTTGCt < 1:2381186/68‑1 (MQ=255) tttGATGACATGAAACCGGAGCATTTTGCATGGAATTTTCGTTTTCACCCAAACGTCTTGTTGTTGCt < 1:2311928/68‑1 (MQ=255) tttGATGACATGAAACCGGAGCATTTTGCATGGAATTTTCGTTTTCACCCAAACGTCTTGTTGTTGCt < 1:2954771/68‑1 (MQ=255) tttGATGACATGAAACCGGAGCATTTTGCATGGAATTTTCGTTTTCACCCAAACGTCTTGTTGTTGCt < 1:1486912/68‑1 (MQ=255) tttGATGACATGAAACCGGAGCATTTTGCATGGAATTTTCGTTTTCACCCAAACGTCTTGTTGTTGCt < 1:488686/68‑1 (MQ=255) tttGATGACATGAAACCGGAGCATTTTGCATGGAATTTTCGTTTTCACCCAAACGTCTTGTTGTTGCt < 1:584050/68‑1 (MQ=255) ttGATGACATGAAACCGGAGCATTTTGCATGGAATTTTCGTTTTCACCCAAACGTCTTGTTGTTGCTGt > 1:3055280/1‑69 (MQ=255) gaCATGAAACCGGAGCATTTTGCATGGAATTTTCGTTTTCACCCAAACGTCTTGTTGTTGCTGTcgcc < 1:438942/68‑1 (MQ=255) cATGAAACCGGAGCATTTTGCATGGAATTTTCGTTTTCACCCAAACTTCTTGTTGTTGCTGTcgccgca > 1:3252984/1‑68 (MQ=255) cATGAAACCGGAGCATTTTGCATGGAATTTTCGTTTTCACCCAAACTTCTTGTTGTTGCTGTcgccgca > 1:542405/1‑68 (MQ=255) cATGAAACCGGAGCATTTTGCATGGAATTTTCGTTTTCACCCAAACTTCTTGTTGTTGCTGTcgccgca > 1:1190030/1‑68 (MQ=255) cATGAAACCGGAGCATTTTGCATGGAATTTTCGTTTTCACCCAAACTTCTTGTTGTTGCTGTcgccgca > 1:2535913/1‑68 (MQ=255) cATGAAACCGGAGCATTTTGCATGGAATTTTCGTTTTCACCCAAACTTCTTGTTGTTGCTGTcgccgc > 1:842133/1‑68 (MQ=255) cATGAAACCGGAGCATTTTGCATGGAATTTTCGTTTTCACCCAAACTTCTTGTTGTTGCTGTcgccgc > 1:277790/1‑68 (MQ=255) aaaCCGGAGCATTTTGCATGGAATTTTCGTTTTCACCCAAACGTCTTGTTGTTGCTGTCGCCGCCGctc > 1:2641741/1‑69 (MQ=255) aaaCCGGAGCATTTTGCATGGAATTTTCGTTTTCACCCAAACGTCTTGTTGTTGCTGTCGCCGCCGctc > 1:3398492/1‑69 (MQ=255) aaaCCGGAGCATTTTGCATGGAATTTTCGTTTTCACCCAAACGTCTTGTTGTTGCTGTCGCCGCCGctc > 1:2645670/1‑69 (MQ=255) aaaCCGGAGCATTTTGCATGGAATTTTCGTTTTCACCCAAACGTCTTGTTGTTGCTGTCGCCGCCGctc > 1:2351872/1‑69 (MQ=255) ccGGAGCATTTTGCATGGAATTTTCGTTTTCACCCAAACGTCTTGTTGTTGCTGTCGCCGCCGCTCTTc < 1:1398450/69‑1 (MQ=255) aGCATTTTGCATGGAATTTTCGTTTTCACCCAAACGTCTTGTTGTTGCTGTCGCCGCCGCTCTTCctct < 1:2819101/69‑1 (MQ=255) aGCATTTTGCATGGAATTTTCGTTTTCACCCAAACGTCTTGTTGTTGCTGTCGCCGCCGCTCTTCctct < 1:1106162/69‑1 (MQ=255) aGCATTTTGCATGGAATTTTCGTTTTCACCCAAACGTCTTGTTGTTGCTGTCGCCGCCGCTCTTCctct < 1:46255/69‑1 (MQ=255) aGCATTTTGCATGGAATTTTCGTTTTCACCCAAACGTCTTGTTGTTGCTGTCGCCGCCGCTCTTCctct < 1:426584/69‑1 (MQ=255) aGCATTTTGCATGGAATTTTCGTTTTCACCCAAACGTCTTGTTGTTGCTGTCGCCGCCGCTCTTCctct < 1:1408950/69‑1 (MQ=255) aGCATTTTGCATGGAATTTTCGTTTTCACCCAAACGTCTTGTTGTTGCTGTCGCCGCCGCTCTTCctct < 1:2304140/69‑1 (MQ=255) ttGCATGGAATTTTCGTTTTCACCCAAACGTCttgttgtt < 1:2621450/40‑1 (MQ=255) ttGCATGGAATTTTCGTTTTCACCCAAACGTCttgttgtt < 1:1697442/40‑1 (MQ=255) ttGCATGGAATTTTCGTTTTCACCCAAACGTCttgttgtt < 1:1194324/40‑1 (MQ=255) ttGCATGGAATTTTCGTTTTCACCCAAACGTCttgttgtt < 1:374733/40‑1 (MQ=255) ttGCATGGAATTTTCGTTTTCACCCAAACGTCTTGTTGTTGCt < 1:610179/43‑1 (MQ=255) ttGCATGGAATTTTCGTTTTCACCCAAACGTCTTGTTGTTGCTGTCGCCGCCGCTCTTCCTCTCATg > 1:1362441/1‑67 (MQ=255) ttttCGTTTTCACCCAAACGTCTTGTTGTTGCTGTCGCCGCCGCTCTTCCTCTCATGGCCAGCgcagca < 1:1980313/69‑1 (MQ=255) tttCGTTTTCACCCAAACGTCTTGTTGTTGCTGTCGCCGCCGCTCTTCCTCTCATGGCCAGCgcagca < 1:1429789/68‑1 (MQ=255) ttCGTTTTCACCCAAACGTCTTGTTGTTGCTGTCGCCGCCGCTCTTCCTCTCATGGCCAGCgcagca < 1:890427/67‑1 (MQ=255) ttCGTTTTCACCCAAACGTCTTGTTGTTGCTGTCGCCGCCGCTCTTCCTCTCATGGCCAGCgcagca < 1:584432/67‑1 (MQ=255) ttCGTTTTCACCCAAACGTCTTGTTGTTGCTGTCGCCGCCGCTCTTCCTCTCATGGCCAGCgcagca < 1:1898195/67‑1 (MQ=255) ttCGTTTTCACCCAAACGTCTTGTTGTTGCTGTCGCCGCCGCTCTTCCTCTCATGGCCAGCgcagca < 1:3087348/67‑1 (MQ=255) ttCGTTTTCACCCAAACGTCTTGTTGTTGCTGTCGCCGCCGCTCTTCCTCTCATGGCCAGCgcagca < 1:2371832/67‑1 (MQ=255) ttCGTTTTCACCCAAACGTCTTGTTGTTGCTGTCGCCGCCGCTCTTCCTCTCATGGCCAGCGCAGCAGa < 1:465792/69‑1 (MQ=255) ttCGTTTTCACCCAAACGTCTTGTTGTTGCTGTCGCCGCCGCTCTTCCTCTCATGGCCAGCGCAGCAGa < 1:275135/69‑1 (MQ=255) ttCGTTTTCACCCAAACGTCTTGTTGTTGCTGTCGCCGCCGCTCTTCCTCTCATGGCCAGCGCAGCAGa < 1:1909196/69‑1 (MQ=255) ttCGTTTTCACCCAAACGTCTTGTTGTTGCTGTCGCCGCCGCTCTTCCTCTCATGGCCAGCGCAGCAGa > 1:2786528/1‑69 (MQ=255) ttCGTTTTCACCCAAACGTCTTGTTGTTGCTGTCGCCGCCGCTCTTCCTCTCATGGCCAGCGCAGCAGa < 1:355039/69‑1 (MQ=255) ttCGTTTTCACCCAAACGTCTTGTTGTTGCTGTCGCCGCCGCTCTTCCTCTCATGGCCAGCGCAGCAGa > 1:2790555/1‑69 (MQ=255) ttCGTTTTCACCCAAACGTCTTGTTGTTGCTGTCGCCGCCGCTCTTCCTCTCATGGCCAGCGCAGCAGa > 1:3262029/1‑69 (MQ=255) ttCGTTTTCACCCAAACGTCTTGTTGTTGCTGTCGCCGCCGCTCTTCCTCTCATGGCCAGCGCAGCAGa < 1:1822228/69‑1 (MQ=255) ttCGTTTTCACCCAAACGTCTTGTTGTTGCTGTCGCCGCCGCTCTTCCTCTCATGGCCAGCGCAGCAGa < 1:1767687/69‑1 (MQ=255) ttCGTTTTCACCCAAACGTCTTGTTGTTGCTGTCGCCGCCGCTCTTCCTCTCATGGCCAGCGCAGCAGa < 1:1755522/69‑1 (MQ=255) ttCGTTTTCACCCAAACGTCTTGTTGTTGCTGTCGCCGCCGCTCTTCCTCTCATGGCCAGCGCAGCAGa < 1:605748/69‑1 (MQ=255) ttCGTTTTCACCCAAACGTCTTGTTGTTGCTGTCGCCGCCGCTCTTCCTCTCATGGCCAGCGCAGCAGa > 1:1073180/1‑69 (MQ=255) ttCGTTTTCACCCAAACGTCTTGTTGTTGCTGTCGCCGCCGCTCTTCCTCTCATGGCCAGCGCAGCAGa < 1:2312476/69‑1 (MQ=255) ttCGTTTTCACCCAAACGTCTTGTTGTTGCTGTCGCCGCCGCGCTTCCTCTCATGGCCAGCGCAGCAGa < 1:2538550/69‑1 (MQ=255) tCGTTTTCACCCAAACGTCTTGTTGTTGCTGTCGCCGCCGCTCTTCCTCTCATGGCCAGCGCAGCAGa < 1:2092804/68‑1 (MQ=255) tCGTTTTCACCCAAACGTCTTGTTGTTGCTGTCGCCGCCGCTCTTCCTCTCATGGCCAGCGCAGCAGa < 1:1264356/68‑1 (MQ=255) ttttCACCCAAACGTCTTGTTGTTGCTGTCGCCGCCGCTCTTCCTCTCATGGCCAGCgcagca < 1:2599382/63‑1 (MQ=255) ttCACCCAAACGTCTTGTTGTTGCTGTCGCCGCCGCTCTTCCTCTCATGGCCAGCGCAGCAGATAcccc > 1:3100164/1‑69 (MQ=255) ttCACCCAAACGTCTTGTTGTTGCTGTCGCCGCCGCTCTTCCTCTCATGGCCAGCGCAGCAGATAccc > 1:1521256/1‑68 (MQ=255) ttCACCCAAACGTCTTGTTGTTGCTGTCGCCGCCGCTCTTCCTCTCATGGCCAGCGCAGCAGATAcc > 1:326179/1‑67 (MQ=255) cACCCAAACTTCTTGTTGTTGCTGTCGCCGCAGCTCTTCCTCTCATAGCCAGCGCAGCAGATACCCCg < 1:2195057/68‑1 (MQ=255) cACCCAAACTTCTTGTTGTTGCTGTCGCCGCAGCTCTTCCTCTCATAGCCAGCGCAGCAGATACCCCg < 1:3205990/68‑1 (MQ=255) cACCCAAACTTCTTGTTGTTGCTGTCGCCGCAGCTCTTCCTCTCATAGCCAGCGCAGCAGATACCCCg < 1:1550988/68‑1 (MQ=255) cACCCAAACTTCTTGTTGTTGCTGTCGCCGCAGCTCTTCCTCTCATAGCCAGCGCAGCAGATACCCCg < 1:2539798/68‑1 (MQ=255) cACCCAAACTTCTTGTTGTTGCTGTCGCCGCAGCTCTTCCTCTCATAGCCAGCGCAGCAGATACCCCg < 1:660999/68‑1 (MQ=255) cACCCAAACTTCTTGTTGTTGCTGTCGCCGCAGCTCTTCCTCTCATAGCCAGCGCAGCAGATACCCCg < 1:1734001/68‑1 (MQ=255) cACCCAAACTTCTTGTTGTTGCTGTCGCCGCAGCTCTTCCTCTCATAGCCAGCGCAGCAGATACCCCg < 1:1678766/68‑1 (MQ=255) cACCCAAACTTCTTGTTGTTGCTGTCGCCGCAGCTCTTCCTCTCATAGCCAGCGCAGCAGATACCCCg < 1:873795/68‑1 (MQ=255) cACCCAAACTTCTTGTTGTTGCTGTCGCCGCAGCTCTTCCTCTCATAGCCAGCGCAGCAGATACCCCg < 1:2257438/68‑1 (MQ=255) aCCCAAACTTCTTGTTGTTGCTGTCGCCGCAGCTCTTCCTCTCATAGCCAGCGCAGCAGATACCCCg < 1:1907313/67‑1 (MQ=255) ccAAACGTCTTGTTGTTGCTGTCGCCGCCGCTCTTCCTCTCATGGCCAGCGCAGCAGATACCCCGTCa < 1:570526/68‑1 (MQ=255) cAAACGTCTTGTTGTTGCTGTCGCCGCCGCTCTTACTCTCATGGCCAGCgcagca < 1:2856553/55‑1 (MQ=255) cGTCTTGTTGTTGCTGTCGCCGCCGCTCTTCCTCTCATGGCCAGCGCAGCAGATACCCCGTCAACTGcc < 1:596100/69‑1 (MQ=255) | ATCATTTTGATGACATGAAACCGGAGCATTTTGCATGGAATTTTCGTTTTCACCCAAACGTCTTGTTGTTGCTGTCGCCGCCGCTCTTCCTCTCATGGCCAGCGCAGCAGATACCCCGTCAACTGCC > minE/2412572‑2412698 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |