| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,412,668 | G→A | 31.8% | M21I (ATG→ATA) | aslA → | acrylsulfatase‑like enzyme |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,412,668 | 0 | G | A | 31.8% | 59.4 / 32.6 | 69 | M21I (ATG→ATA) | aslA | acrylsulfatase‑like enzyme |
| Reads supporting (aligned to +/- strand): ref base G (8/39); new base A (12/10); total (20/49) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.47e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.28e-01 | |||||||||||
TTGCATGGAATTTTCGTTTTCACCCAAACGTCTTGTTGTTGCTGTCGCCGCCGCTCTTCCTCTCATGGCCAGCGCAGCAGATACCCCGTCAACTGCCACCGCACGCAAAGGCTTTGCCGGATACG > minE/2412602‑2412726 | ttGCATGGAATTTTCGTTTTCACCCAAACGTCTTGTTGTTGCTGTCGCCGCCGCTCTTCCTCTCATg > 1:1362441/1‑67 (MQ=255) ttttCGTTTTCACCCAAACGTCTTGTTGTTGCTGTCGCCGCCGCTCTTCCTCTCATGGCCAGCgcagca < 1:1980313/69‑1 (MQ=255) tttCGTTTTCACCCAAACGTCTTGTTGTTGCTGTCGCCGCCGCTCTTCCTCTCATGGCCAGCgcagca < 1:1429789/68‑1 (MQ=255) ttCGTTTTCACCCAAACGTCTTGTTGTTGCTGTCGCCGCCGCTCTTCCTCTCATGGCCAGCgcagca < 1:2371832/67‑1 (MQ=255) ttCGTTTTCACCCAAACGTCTTGTTGTTGCTGTCGCCGCCGCTCTTCCTCTCATGGCCAGCgcagca < 1:959744/67‑1 (MQ=255) ttCGTTTTCACCCAAACGTCTTGTTGTTGCTGTCGCCGCCGCTCTTCCTCTCATGGCCAGCgcagca < 1:890427/67‑1 (MQ=255) ttCGTTTTCACCCAAACGTCTTGTTGTTGCTGTCGCCGCCGCTCTTCCTCTCATGGCCAGCgcagca < 1:584432/67‑1 (MQ=255) ttCGTTTTCACCCAAACGTCTTGTTGTTGCTGTCGCCGCCGCTCTTCCTCTCATGGCCAGCgcagca < 1:3087348/67‑1 (MQ=255) ttCGTTTTCACCCAAACGTCTTGTTGTTGCTGTCGCCGCCGCTCTTCCTCTCATGGCCAGCgcagca < 1:1898195/67‑1 (MQ=255) ttCGTTTTCACCCAAACGTCTTGTTGTTGCTGTCGCCGCCGCTCTTCCTCTCATGGCCAGCGCAGCAGa < 1:1822228/69‑1 (MQ=255) ttCGTTTTCACCCAAACGTCTTGTTGTTGCTGTCGCCGCCGCTCTTCCTCTCATGGCCAGCGCAGCAGa < 1:2312476/69‑1 (MQ=255) ttCGTTTTCACCCAAACGTCTTGTTGTTGCTGTCGCCGCCGCTCTTCCTCTCATGGCCAGCGCAGCAGa > 1:3262029/1‑69 (MQ=255) ttCGTTTTCACCCAAACGTCTTGTTGTTGCTGTCGCCGCCGCTCTTCCTCTCATGGCCAGCGCAGCAGa < 1:355039/69‑1 (MQ=255) ttCGTTTTCACCCAAACGTCTTGTTGTTGCTGTCGCCGCCGCTCTTCCTCTCATGGCCAGCGCAGCAGa < 1:465792/69‑1 (MQ=255) ttCGTTTTCACCCAAACGTCTTGTTGTTGCTGTCGCCGCCGCTCTTCCTCTCATGGCCAGCGCAGCAGa > 1:1073180/1‑69 (MQ=255) ttCGTTTTCACCCAAACGTCTTGTTGTTGCTGTCGCCGCCGCTCTTCCTCTCATGGCCAGCGCAGCAGa < 1:1909196/69‑1 (MQ=255) ttCGTTTTCACCCAAACGTCTTGTTGTTGCTGTCGCCGCCGCTCTTCCTCTCATGGCCAGCGCAGCAGa < 1:1767687/69‑1 (MQ=255) ttCGTTTTCACCCAAACGTCTTGTTGTTGCTGTCGCCGCCGCTCTTCCTCTCATGGCCAGCGCAGCAGa < 1:1755522/69‑1 (MQ=255) ttCGTTTTCACCCAAACGTCTTGTTGTTGCTGTCGCCGCCGCTCTTCCTCTCATGGCCAGCGCAGCAGa < 1:275135/69‑1 (MQ=255) ttCGTTTTCACCCAAACGTCTTGTTGTTGCTGTCGCCGCCGCTCTTCCTCTCATGGCCAGCGCAGCAGa < 1:605748/69‑1 (MQ=255) ttCGTTTTCACCCAAACGTCTTGTTGTTGCTGTCGCCGCCGCTCTTCCTCTCATGGCCAGCGCAGCAGa > 1:2786528/1‑69 (MQ=255) ttCGTTTTCACCCAAACGTCTTGTTGTTGCTGTCGCCGCCGCTCTTCCTCTCATGGCCAGCGCAGCAGa > 1:2790555/1‑69 (MQ=255) ttCGTTTTCACCCAAACGTCTTGTTGTTGCTGTCGCCGCCGCGCTTCCTCTCATGGCCAGCGCAGCAGa < 1:2538550/69‑1 (MQ=255) tCGTTTTCACCCAAACGTCTTGTTGTTGCTGTCGCCGCCGCTCTTCCTCTCATGGCCAGCGCAGCAGa < 1:1264356/68‑1 (MQ=255) tCGTTTTCACCCAAACGTCTTGTTGTTGCTGTCGCCGCCGCTCTTCCTCTCATGGCCAGCGCAGCAGa < 1:2092804/68‑1 (MQ=255) ttttCACCCAAACGTCTTGTTGTTGCTGTCGCCGCCGCTCTTCCTCTCATGGCCAGCgcagca < 1:2599382/63‑1 (MQ=255) ttCACCCAAACGTCTTGTTGTTGCTGTCGCCGCCGCTCTTCCTCTCATGGCCAGCGCAGCAGATAcccc > 1:1818228/1‑69 (MQ=255) ttCACCCAAACGTCTTGTTGTTGCTGTCGCCGCCGCTCTTCCTCTCATGGCCAGCGCAGCAGATAcccc > 1:3100164/1‑69 (MQ=255) ttCACCCAAACGTCTTGTTGTTGCTGTCGCCGCCGCTCTTCCTCTCATGGCCAGCGCAGCAGATAccc > 1:1521256/1‑68 (MQ=255) ttCACCCAAACGTCTTGTTGTTGCTGTCGCCGCCGCTCTTCCTCTCATGGCCAGCGCAGCAGATAcc > 1:326179/1‑67 (MQ=255) cACCCAAACTTCTTGTTGTTGCTGTCGCCGCAGCTCTTCCTCTCATAGCCAGCGCAGCAGATACCCCg < 1:3205990/68‑1 (MQ=255) cACCCAAACTTCTTGTTGTTGCTGTCGCCGCAGCTCTTCCTCTCATAGCCAGCGCAGCAGATACCCCg < 1:873795/68‑1 (MQ=255) cACCCAAACTTCTTGTTGTTGCTGTCGCCGCAGCTCTTCCTCTCATAGCCAGCGCAGCAGATACCCCg < 1:2539798/68‑1 (MQ=255) cACCCAAACTTCTTGTTGTTGCTGTCGCCGCAGCTCTTCCTCTCATAGCCAGCGCAGCAGATACCCCg < 1:2195057/68‑1 (MQ=255) cACCCAAACTTCTTGTTGTTGCTGTCGCCGCAGCTCTTCCTCTCATAGCCAGCGCAGCAGATACCCCg < 1:660999/68‑1 (MQ=255) cACCCAAACTTCTTGTTGTTGCTGTCGCCGCAGCTCTTCCTCTCATAGCCAGCGCAGCAGATACCCCg < 1:1550988/68‑1 (MQ=255) cACCCAAACTTCTTGTTGTTGCTGTCGCCGCAGCTCTTCCTCTCATAGCCAGCGCAGCAGATACCCCg < 1:1678766/68‑1 (MQ=255) cACCCAAACTTCTTGTTGTTGCTGTCGCCGCAGCTCTTCCTCTCATAGCCAGCGCAGCAGATACCCCg < 1:2257438/68‑1 (MQ=255) cACCCAAACTTCTTGTTGTTGCTGTCGCCGCAGCTCTTCCTCTCATAGCCAGCGCAGCAGATACCCCg < 1:1734001/68‑1 (MQ=255) aCCCAAACTTCTTGTTGTTGCTGTCGCCGCAGCTCTTCCTCTCATAGCCAGCGCAGCAGATACCCCg < 1:1907313/67‑1 (MQ=255) ccAAACGTCTTGTTGTTGCTGTCGCCGCCGCTCTTCCTCTCATGGCCAGCGCAGCAGATACCCCGTCa < 1:570526/68‑1 (MQ=255) cAAACGTCTTGTTGTTGCTGTCGCCGCCGCTCTTACTCTCATGGCCAGCgcagca < 1:2856553/55‑1 (MQ=255) cGTCTTGTTGTTGCTGTCGCCGCCGCTCTTCCTCTCATGGCCAGCGCAGCAGATACCCCGTCAACTGcc < 1:596100/69‑1 (MQ=255) tgCTGTCGCCGCCGCTCTTCCTCTCATGGCCAGCGCAGCAGATACCCCGTCAACTGCCACcgcacgca < 1:2595533/68‑1 (MQ=255) tgCTGTCGCCGCCGCTCTTCCTCTCATGGCCAGCGCAGCAGATACCCCGTCAACTGCCACcgcacgca < 1:2571554/68‑1 (MQ=255) tgCTGTCGCCGCCGCTCTTCCTCTCATGGCCAGCGCAGCAGATACCCCGTCAACTGCCACcgcacgca < 1:1288156/68‑1 (MQ=255) tgCTGTCGCCGCCGCTCTTCCTCTCATGGCCAGCGCAGCAGATACCCCGTCAACTGCCACcgcacgca < 1:194377/68‑1 (MQ=255) tgCTGTCGCCGCCGCTCTTCCTCTCATGGCCAGCGCAGCAGATACCCCGTCAACTGCCACcgcacgca < 1:2428542/68‑1 (MQ=255) tgCTGTCGCCGCCGCTCTTCCTCTCATGGCCAGCGCAGCAGATACCCCGTCAACTGCCACcgcacgca < 1:2836132/68‑1 (MQ=255) gCTGTCGCCGCCGCTCTTCCTCTCATGGCCAGCGCAGCAGa < 1:1391478/41‑1 (MQ=255) ctctTCCTCTCATGGCCAGCGCAGCAGATACCCCGTCAACTGCCACCGCACGCAAAGGCTTTGCCGGAt < 1:109176/69‑1 (MQ=255) ttCCTCTCATGGCCAGCGCAGCAGATACCCCGTCAACTGCCACcgcacgca < 1:1078471/51‑1 (MQ=255) ccTCTCATGGCCAGCGCAGCAGATACCCCGTCAACTGCCACCGCACGCAAAGGCTTTGCCGGATACg < 1:1098227/67‑1 (MQ=255) ccTCTCATGGCCAGCGCAGCAGATACCCCGTCAACTGCCACCGCACGCAAAGGCTTTGCCGGATACg < 1:895225/67‑1 (MQ=255) ccTCTCATGGCCAGCGCAGCAGATACCCCGTCAACTGCCACCGCACGCAAAGGCTTTGCCGGATACg < 1:1373760/67‑1 (MQ=255) ccTCTCATGGCCAGCGCAGCAGATACCCCGTCAACTGCCACCGCACGCAAAGGCTTTGCCGGATACg < 1:847897/67‑1 (MQ=255) ccTCTCATGGCCAGCGCAGCAGATACCCCGTCAACTGCCACCGCACGCAAAGGCTTTGCCGGATACg < 1:2479877/67‑1 (MQ=255) ccTCTCATGGCCAGCGCAGCAGATACCCCGTCAACTGCCACCGCACGCAAAGGCTTTGCCGGATACg < 1:2572331/67‑1 (MQ=255) cATAGCCAGCGCAGCAGATACCCCGTCAACCGCCACcgca > 1:2327553/1‑40 (MQ=38) cATAGCCAGCGCAGCAGATACCCCGTCAACCGCCACcgca > 1:2167918/1‑40 (MQ=38) cATAGCCAGCGCAGCAGATACCCCGTCAACCGCCACcgca > 1:1964561/1‑40 (MQ=38) cATAGCCAGCGCAGCAGATACCCCGTCAACCGCCACcgca > 1:1945796/1‑40 (MQ=38) cATAGCCAGCGCAGCAGATACCCCGTCAACCGCCACcgca > 1:2824330/1‑40 (MQ=38) cATAGCCAGCGCAGCAGATACCCCGTCAACCGCCACcgca > 1:1785677/1‑40 (MQ=38) cATAGCCAGCGCAGCAGATACCCCGTCAACCGCCACcgca > 1:1621358/1‑40 (MQ=38) cATAGCCAGCGCAGCAGATACCCCGTCAACCGCCACcgca > 1:1336678/1‑40 (MQ=38) cATAGCCAGCGCAGCAGATACCCCGTCAACCGCCACcgca > 1:3223864/1‑40 (MQ=38) cATAGCCAGCGCAGCAGATACCCCGTCAACCGCCACcgca > 1:277152/1‑40 (MQ=38) cATAGCCAGCGCAGCAGATACCCCGTCAACCGCCACcgca > 1:1164192/1‑40 (MQ=38) cATAGCCAGCGCAGCAGATACCCCGTCAACCGCCACcgc > 1:893928/1‑39 (MQ=38) | TTGCATGGAATTTTCGTTTTCACCCAAACGTCTTGTTGTTGCTGTCGCCGCCGCTCTTCCTCTCATGGCCAGCGCAGCAGATACCCCGTCAACTGCCACCGCACGCAAAGGCTTTGCCGGATACG > minE/2412602‑2412726 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |