| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,413,154 | T→C | 100% | T183T (ACT→ACC) | aslA → | acrylsulfatase‑like enzyme |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,413,154 | 0 | T | C | 76.6% | 41.5 / 7.2 | 34 | T183T (ACT→ACC) | aslA | acrylsulfatase‑like enzyme |
| Reads supporting (aligned to +/- strand): ref base T (5/3); new base C (26/0); total (31/3) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.36e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GGCTGCAAGGGTTAACCACGCTGCCGCAGTTGCTGCACGATCAGGGCTACGTCACTCAGGCCATCGGAAAATGGCATATGGGGGAAAACAAAGAGTCGCAGCCGCAGAACG > minE/2413099‑2413209 | ggcTGCAAGGGTTAACCACGCTGCCGCAGTTGCTGCACGATCAGGGCTACGTCACTCAGGCCATCGGaa < 1:589210/69‑1 (MQ=255) gTTAACCACGCTGCCGCAGTTGCTGCACGATCAGGGCTACGTCACTCAGGCCATCGGAAAATGGCa < 1:3121766/66‑1 (MQ=255) gTTAACCACGCTGCCGCAGTTGCTGCACGATCAGGGCTACGTCACTCAGGCCATCGGAAAATGGCa < 1:213376/66‑1 (MQ=255) gctgcACGATCAGGGCTACGTCACTCAGGCCATCGGAAAATGGCATATGGGGGAAAACAAAGAGTCGCa > 1:1077421/1‑69 (MQ=255) gctgcACGATCAGGGCTACGTCACTCAGGCCATCGGAAAATGGCATATGGGGGAAAACAAAGAGTCGCa > 1:2678672/1‑69 (MQ=255) gctgcACGATCAGGGCTACGTCACTCAGGCCATCGGAAAATGGCATATGGGGGAAAACAAAGAGTCGCa > 1:2506609/1‑69 (MQ=255) gctgcACGATCAGGGCTACGTCACTCAGGCCATCGGAAAATGGCATATGGGGGAAAACAAAGAGTCGCa > 1:1801093/1‑69 (MQ=255) gctgcACGATCAGGGCTACGTCACTCAGGCCATCGGAAAATGGCATATGGGGGAAAACAAAGAGTCGCa > 1:1638567/1‑69 (MQ=255) gctgcACGATCAGGGCTACGTCACCCAGGCTATCGGGAAATGGCATATGGGGGAAAACAAag > 1:1672778/1‑62 (MQ=255) gctgcACGATCAGGGCTACGTCACCCAGGCTATCGGGAAATGGCATATGGGGGAAAACAAAGAGTCGc > 1:3382792/1‑68 (MQ=255) gctgcACGATCAGGGCTACGTCACCCAGGCTATCGGGAAATGGCATATGGGGGAAAACAAAGAGTCGCa > 1:847383/1‑69 (MQ=255) gctgcACGATCAGGGCTACGTCACCCAGGCTATCGGGAAATGGCATATGGGGGAAAACAAAGAGTCGCa > 1:294642/1‑69 (MQ=255) gctgcACGATCAGGGCTACGTCACCCAGGCTATCGGGAAATGGCATATGGGGGAAAACAAAGAGTCGCa > 1:272549/1‑69 (MQ=255) gctgcACGATCAGGGCTACGTCACCCAGGCTATCGGGAAATGGCATATGGGGGAAAACAAAGAGTCGCa > 1:1194699/1‑69 (MQ=255) gctgcACGATCAGGGCTACGTCACCCAGGCTATCGGGAAATGGCATATGGGGGAAAACAAAGAGTCGCa > 1:891100/1‑69 (MQ=255) gctgcACGATCAGGGCTACGTCACCCAGGCTATCGGGAAATGGCATATGGGGGAAAACAAAGAGTCGCa > 1:2299279/1‑69 (MQ=255) gctgcACGATCAGGGCTACGTCACCCAGGCTATCGGGAAATGGCATATGGGGGAAAACAAAGAGTCGCa > 1:1454061/1‑69 (MQ=255) gctgcACGATCAGGGCTACGTCACCCAGGCTATCGGGAAATGGCATATGGGGGAAAACAAAGAGTCGCa > 1:94524/1‑69 (MQ=255) gctgcACGATCAGGGCTACGTCACCCAGGCTATAGGGAAATGGCATATGGGGGAAAACAAAGAGTCGCa > 1:1004574/1‑69 (MQ=255) gggCTACGTCACCCAGGCTATCGGGAAATGGCATATGGGGGAAAACAAAGAGTCGCAGCCGCAGAAc > 1:3247156/1‑67 (MQ=255) gggCTACGTCACCCAGGCTATCGGGAAATGGCATATGGGGGAAAACAAAGAGTCGCAGCCGCAGAACg > 1:992970/1‑68 (MQ=255) gggCTACGTCACCCAGGCTATCGGGAAATGGCATATGGGGGAAAACAAAGAGTCGCAGCCGCAGAACg > 1:612323/1‑68 (MQ=255) gggCTACGTCACCCAGGCTATCGGGAAATGGCATATGGGGGAAAACAAAGAGTCGCAGCCGCAGAACg > 1:905297/1‑68 (MQ=255) gggCTACGTCACCCAGGCTATCGGGAAATGGCATATGGGGGAAAACAAAGAGTCGCAGCCGCAGAACg > 1:1660303/1‑68 (MQ=255) gggCTACGTCACCCAGGCTATCGGGAAATGGCATATGGGGGAAAACAAAGAGTCGCAGCCGCAGAACg > 1:3370576/1‑68 (MQ=255) gggCTACGTCACCCAGGCTATCGGGAAATGGCATATGGGGGAAAACAAAGAGTCGCAGCCGCAGAACg > 1:1115448/1‑68 (MQ=255) gggCTACGTCACCCAGGCTATCGGGAAATGGCATATGGGGGAAAACAAAGAGTCGCAGCCGCAGAACg > 1:2778847/1‑68 (MQ=255) gggCTACGTCACCCAGGCTATCGGGAAATGGCATATGGGGGAAAACAAAGAGTCGCAGCCGCAGAACg > 1:2743091/1‑68 (MQ=255) gggCTACGTCACCCAGGCTATCGGGAAATGGCATATGGGGGAAAACAAAGAGTCGCAGCCGCAGAACg > 1:1324756/1‑68 (MQ=255) gggCTACGTCACCCAGGCTATCGGGAAATGGCATATGGGGGAAAACAAAGAGTCGCAGCCGCAGAACg > 1:2474120/1‑68 (MQ=255) gggCTACGTCACCCAGGCTATCGGGAAATGGCATATGGGGGAAAACAAAGAGTCGCAGCCGCAGAACg > 1:2358662/1‑68 (MQ=255) gggCTACGTCACCCAGGCTATCGGGAAATGGCATATGGGGGAAAACAAAGAGTCGCAGCCGCAGAACg > 1:2096962/1‑68 (MQ=255) gggCTACGTCACCCAGGCTATCGGGAAATGGCATATGGGGGAAAACAAAGAGTCGCAGCCGCAGAACg > 1:1883361/1‑68 (MQ=255) gggCTACGTCACCCAGGCTATCGGGAAATGGCATATGGGGGAAAACAAAGAGTCGCAGCCGCAGAACg > 1:1803709/1‑68 (MQ=255) | GGCTGCAAGGGTTAACCACGCTGCCGCAGTTGCTGCACGATCAGGGCTACGTCACTCAGGCCATCGGAAAATGGCATATGGGGGAAAACAAAGAGTCGCAGCCGCAGAACG > minE/2413099‑2413209 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |