| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 201,711 | C→T | 32.0% | V18V (GTC→GTT) | gmhB → | D,D‑heptose 1,7‑bisphosphate phosphatase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 201,711 | 0 | C | T | 32.0% | 76.1 / 40.4 | 84 | V18V (GTC→GTT) | gmhB | D,D‑heptose 1,7‑bisphosphate phosphatase |
| Reads supporting (aligned to +/- strand): ref base C (29/28); new base T (21/6); total (50/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.12e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.06e-01 | |||||||||||
GGAGCTATAAAAGGTGGCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGC > minE/201645‑201775 | ggAGCTATAAAAGGTGGCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCg < 1:409917/68‑1 (MQ=255)ggAGCTATAAAAGGTGGCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCg < 1:3380534/68‑1 (MQ=255)ggAGCTATAAAAGGTGGCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCg < 1:2096753/68‑1 (MQ=255) gAGCTATAAAAGGTGGCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCg < 1:758572/67‑1 (MQ=255) gAGCTATAAAAGGTGGCGAAGAGAGTACCCGCAATTTTTCTTGAGCGTGATGGCACCATTAATGTCg < 1:193742/67‑1 (MQ=255) ataAAAGGTGGCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACg > 1:1747947/1‑68 (MQ=255) ataAAAGGTGGCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACg > 1:1243783/1‑68 (MQ=255) ataAAAGGTGGCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACg > 1:1593486/1‑68 (MQ=255) ataAAAGGTGGCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACg > 1:1288178/1‑68 (MQ=255) aaaGGTGGCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGc < 1:1731457/67‑1 (MQ=255) aaaGGTGGCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGc < 1:2735871/67‑1 (MQ=255) aaaGGTGGCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGc < 1:1768720/67‑1 (MQ=255) aaaGGTGGCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGc < 1:262895/67‑1 (MQ=255) aaaGGTGGCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGc < 1:2853224/67‑1 (MQ=255) aaaGGTGGCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGc < 1:3164361/67‑1 (MQ=255) aaaGGTGGCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGc < 1:646348/67‑1 (MQ=255) aaaGGTGGCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGc < 1:862791/67‑1 (MQ=255) aaaGGTGGCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGc < 1:915095/67‑1 (MQ=255) aaaGGTGGCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGc < 1:967716/67‑1 (MQ=255) ggTTGCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTTGATCACGGCTATg > 1:106372/1‑68 (MQ=255) ggTGGCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTTGATCACGGCTAt > 1:1469153/1‑67 (MQ=255) ggTGGCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTTGATCACGGCTATg > 1:303313/1‑68 (MQ=255) ggTGGCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTTGATCACGGCTATg > 1:936707/1‑68 (MQ=255) ggTGGCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTTGATCACGGCTATg > 1:1147991/1‑68 (MQ=255) ggTGGCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTTGATCACGGCTATg > 1:864294/1‑68 (MQ=255) ggTGGCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTTGATCACGGCTATg > 1:805308/1‑68 (MQ=255) ggTGGCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTTGATCACGGCTATg > 1:726222/1‑68 (MQ=255) ggTGGCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTTGATCACGGCTATg > 1:456696/1‑68 (MQ=255) ggTGGCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTTGATCACGGCTATg > 1:3002658/1‑68 (MQ=255) ggTGGCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTTGATCACGGCTATg > 1:2920028/1‑68 (MQ=255) ggTGGCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTTGATCACGGCTATg > 1:2841483/1‑68 (MQ=255) ggTGGCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTTGATCACGGCTATg > 1:1018849/1‑68 (MQ=255) ggTGGCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTTGATCACGGCTATg > 1:2733261/1‑68 (MQ=255) ggTGGCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTTGATCACGGCTATg > 1:2324645/1‑68 (MQ=255) ggTGGCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTTGATCACGGCTATg > 1:219567/1‑68 (MQ=255) ggTGGCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTTGATCACGGCTATg > 1:2003379/1‑68 (MQ=255) ggTGGCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTTGATCACGGCTATg > 1:1800797/1‑68 (MQ=255) ggTGGCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTTGATCACGGCTATg > 1:1996438/1‑68 (MQ=255) ggTGGCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTTGATCACGGCTATg > 1:1744421/1‑68 (MQ=255) ggTGGCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTTGATAACGGCTAt > 1:1218725/1‑67 (MQ=255) gCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTcc > 1:1287318/1‑67 (MQ=255) gCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTcc > 1:3430468/1‑67 (MQ=255) aaGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGc < 1:3359509/57‑1 (MQ=255) gTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGAc < 1:2001256/69‑1 (MQ=255) cccGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGc < 1:525369/48‑1 (MQ=255) cccGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGc < 1:1266939/48‑1 (MQ=255) cccGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGc < 1:1981056/48‑1 (MQ=255) cccGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGc < 1:2665577/48‑1 (MQ=255) cccGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGc < 1:2786565/48‑1 (MQ=255) cccGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAAc > 1:2073087/1‑69 (MQ=255) cccGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAAc > 1:1434083/1‑69 (MQ=255) cGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATc > 1:2335693/1‑41 (MQ=255) tttCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAAttt > 1:380135/1‑69 (MQ=255) tttCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAAttt > 1:3333710/1‑69 (MQ=255) tttCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAAttt > 1:169585/1‑69 (MQ=255) tttCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAAttt > 1:60441/1‑69 (MQ=255) tttCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAAttt > 1:314381/1‑69 (MQ=255) tttCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAAttt > 1:1231992/1‑69 (MQ=255) tttCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAAttt > 1:3047224/1‑69 (MQ=255) tttCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAAttt > 1:1976424/1‑69 (MQ=255) tttCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAAttt > 1:93757/1‑69 (MQ=255) tttCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAAtt > 1:1646654/1‑68 (MQ=255) tttCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAAtt > 1:1414502/1‑68 (MQ=255) tttCTTGACCGTGATGGCACCATTAATGTCGATAACGGCTATGTCCATGAGATCGACAACTTTGAAtt > 1:837669/1‑68 (MQ=255) ttGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATc < 1:2929374/68‑1 (MQ=255) ttGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATc < 1:1314247/68‑1 (MQ=255) ttGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATc < 1:170017/68‑1 (MQ=255) tGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGgtgt < 1:2103242/69‑1 (MQ=255) tGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGgtgt < 1:4543/69‑1 (MQ=255) gATGGCACCATTAATGTTGATCACGGCTATGTCCATTAGATCGACAACTTTGAATTTATCGACGGTGtt < 1:1755677/69‑1 (MQ=255) gATGGCACCATTAATGTTGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGtt < 1:1399799/69‑1 (MQ=255) gATGGCACCATTAATGTTGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGtt < 1:3157754/69‑1 (MQ=255) gATGGCACCATTAATGTTGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGtt < 1:3121822/69‑1 (MQ=255) gATGGCACCATTAATGTTGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGtt < 1:845096/69‑1 (MQ=255) gATGGCACCATTAATGTTGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGtt < 1:3056942/69‑1 (MQ=255) gATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGtt < 1:3275772/69‑1 (MQ=255) aTGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACgg > 1:1451538/1‑64 (MQ=255) aTGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACgg > 1:763855/1‑64 (MQ=255) aTGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACgg > 1:592056/1‑64 (MQ=255) aTGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACgg > 1:1715807/1‑64 (MQ=255) aTGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACgg > 1:2968946/1‑64 (MQ=255) aTGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACg > 1:1884847/1‑63 (MQ=255) aaTGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATg > 1:3313921/1‑69 (MQ=255) aTGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATgc > 1:298345/1‑69 (MQ=255) | GGAGCTATAAAAGGTGGCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGC > minE/201645‑201775 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |