BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,416,428	A→G	40.5%	V433V (GTT→GTC)	yifK ←	predicted transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,416,428	0	A	G	40.5%	43.2 / 76.7	106	V433V (GTT→GTC)	yifK	predicted transporter
Reads supporting (aligned to +/- strand): ref base A (29/34); new base G (18/25); total (47/59)
Fisher's exact test for biased strand distribution p-value = 6.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.12e-01

Only 100 of 107 total aligned reads displayed.

GCGATTAAGGCCAAAAACTTTATAAATCGCCGTCACCGCCAGCATAAAGATGATGCCAACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCGATCAAAACGCAAATCAGGAATGCC  >  minE/2416371‑2416494

gCGATTAAGGCCAAAAACTTTATAAATCGCCGTCACCGCCAGCATAAAGATGATGCcaacaa                                                                >  1:2737078/1‑62 (MQ=255)

gCGATTAAGGCCAAAAACTTTATAAATCGCCGTCACCGCCAGCATAAAGATGATGCCAACAAACAGCg                                                          >  1:3173859/1‑68 (MQ=255)

gCGATTAAGGCCAAAAACTTTATAAATCGCCGTCACCGCCAGCATAAAGATGATGCCAACAAACAGCGa                                                         >  1:708907/1‑69 (MQ=255)

gCGATTAAGGCCAAAAACTTTATAAATCGCCGTCACCGCCAGCATAAAGATGATGCCAACAAACAGCGa                                                         >  1:2996895/1‑69 (MQ=255)

gCGATTAAGGCCAAAAACTTTATAAATCGCCGTCACCGCCAGCATAAAGATGATGCCAACAAACAGCGa                                                         >  1:1067114/1‑69 (MQ=255)

gCGATTAAGGCCAAAAACTTTATAAATCGCCGTCACCGCCAGCATAAAGATGATGCCAACAAACAGCGa                                                         >  1:2302054/1‑69 (MQ=255)

gCGATTAAGGCCAAAAACTTTATAAATCGCCGTCACCGCCAGCATAAAGATGATGCCAACAAACAGCGa                                                         >  1:3317196/1‑69 (MQ=255)

         gCCAAAAACTTTATAAATCGCCGTCACCGCCAGCATAAAGATGATGCCAACAAACAGCGACATACGCGt                                                <  1:1108180/69‑1 (MQ=255)

         gCCAAAAACTTTATAAATCGCCGTCACCGCCAGCATAAAGATGATGCCAACAAACAGCGACATACGCGt                                                <  1:3230078/69‑1 (MQ=255)

         gCCAAAAACTTTATAAATCGCCGTCACCGCCAGCATAAAGATGATGCCAACAAACAGCGACATACGCGt                                                <  1:3207909/69‑1 (MQ=255)

         gCCAAAAACTTTATAAATCGCCGTCACCGCCAGCATAAAGATGATGCCAACAAACAGCGACATACGCGt                                                <  1:2293208/69‑1 (MQ=255)

                        aaTCGCCGTCACCGCCAGCATAAAGATGATGCCAACAAACAGCGACATACGCGTATCTTCATTAAAGt                                  <  1:2174302/68‑1 (MQ=255)

                        aaTCGCCGTCACCGCCAGCATAAAGATGATGCCAACAAACAGCGACATACGCGTATCTTCATTAAAGt                                  <  1:3161529/68‑1 (MQ=255)

                        aaTCGCCGTCACCGCCAGCATAAAGATGATGCCAACAAACAGCGACATACGCGTATCTTCATTAAAGt                                  <  1:504283/68‑1 (MQ=255)

                        aaTCGCCGTCACCGCCAGCATAAAGATGATGCCAACAAACAGCGACATACGCGTATCTTCATTAAAGTa                                 <  1:1142914/69‑1 (MQ=255)

                        aaTCGCCGTCACCGCCAGCATAAAGATGATGCCAACAAACAGCGACATACGCGTATCTTCATTAAAGTa                                 <  1:588787/69‑1 (MQ=255)

                        aaTCGCCGTCACCGCCAGCATAAAGATGATGCCAACAAACAGCGACATACGCGTATCTTCATTAAAGTa                                 <  1:2038280/69‑1 (MQ=255)

                        aaTCGCCGTCACCGCCAGCATAAAGATGATGCCAACAAACAGCGACATACGCGTATCTTCATTAAAGTa                                 <  1:3039090/69‑1 (MQ=255)

                        aaTCGCCGTCACCGCCAGCATAAAGATGATGCCAACAAACAGCGACATACGCGTATCTTCATTAAAGTa                                 <  1:2175219/69‑1 (MQ=255)

                        aaTCGCCGTCACCGCCAGCATAAAGATGATGCCAACAAACAGCGACATACGCGTATCTTCATTAAAGTa                                 <  1:2430118/69‑1 (MQ=255)

                               gTCACCACCAGCATGAAGATGATGCCGACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCg                          >  1:3199654/1‑69 (MQ=38)

                               gTCACCACCAGCATAAAGATGATGCCGACAAACAGCGACATGCGCGTATCTTCATTAAAGTACATGCCg                          >  1:1231890/1‑69 (MQ=255)

                               gTCACCACCAGCATAAAGATGATGCCGACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCg                          >  1:2011772/1‑69 (MQ=255)

                               gTCACCACCAGCATAAAGATGATGCCGACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCg                          >  1:1803714/1‑69 (MQ=255)

                               gTCACCACCAGCATAAAGATGATGCCGACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCg                          >  1:1998110/1‑69 (MQ=255)

                               gTCACCACCAGCATAAAGATGATGCCGACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCg                          >  1:1853960/1‑69 (MQ=255)

                               gTCACCACCAGCATAAAGATGATGCCGACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCg                          >  1:1041032/1‑69 (MQ=255)

                               gTCACCACCAGCATAAAGATGATGCCGACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCg                          >  1:227917/1‑69 (MQ=255)

                               gTCACCACCAGCATAAAGATGATGCCGACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCg                          >  1:2413419/1‑69 (MQ=255)

                               gTCACCACCAGCATAAAGATGATGCCGACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCg                          >  1:685925/1‑69 (MQ=255)

                               gTCACCACCAGCATAAAGATGATGCCGACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCg                          >  1:700242/1‑69 (MQ=255)

                               gTCACCACCAGCATAAAGATGATGCCGACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCg                          >  1:744235/1‑69 (MQ=255)

                               gTCACCACCAGCATAAAGATGATGCCGACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCg                          >  1:1100072/1‑69 (MQ=255)

                               gTCACCACCAGCATAAAGATGATGCCGACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCg                          >  1:1393483/1‑69 (MQ=255)

                               gTCACCACCAGCATAAAGATGATGCCGACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCg                          >  1:1246316/1‑69 (MQ=255)

                               gTCACCACCAGCATAAAGATGATGCCGACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCg                          >  1:1122509/1‑69 (MQ=255)

                               gTCACCACCAGCATAAAGATGATGCCGACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCg                          >  1:1080373/1‑69 (MQ=255)

                                  aCCGCCAGCATAAAGATGATGCCAACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCGa                         >  1:326739/1‑67 (MQ=255)

                                  aCCGCCAGCATAAAGATGATGCCAACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCGAt                        >  1:3011583/1‑68 (MQ=255)

                                  aCCGCCAGCATAAAGATGATGCCAACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCGAt                        >  1:1119876/1‑68 (MQ=255)

                                  aCCGCCAGCATAAAGATGATGCCAACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCGAt                        >  1:2993186/1‑68 (MQ=255)

                                  aCCGCCAGCATAAAGATGATGCCAACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCGAt                        >  1:1522842/1‑68 (MQ=255)

                                  aCCGCCAGCATAAAGATGATGCCAACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCGAt                        >  1:3204486/1‑68 (MQ=255)

                                  aCCGCCAGCATAAAGATGATGCCAACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCGAt                        >  1:100299/1‑68 (MQ=255)

                                  aCCGCCAGCATAAAGATGATGCCAACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCGAt                        >  1:1491723/1‑68 (MQ=255)

                                              aaGATTATGCCAACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCGATCAAAACGCAAATc           >  1:1390209/1‑69 (MQ=255)

                                              aaGATGATGCCGACAAACAGCGACATACGCGTATCTTCTTTAAAGTACATGCCGATCAAAACg                 <  1:450425/63‑1 (MQ=39)

                                              aaGATGATGCCGACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCGATCTAAACg                 <  1:1830597/63‑1 (MQ=255)

                                              aaGATGATGCCGACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCGATCAAAACg                 <  1:1237926/63‑1 (MQ=255)

                                              aaGATGATGCCGACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCGATCAAAACg                 <  1:2868114/63‑1 (MQ=255)

                                              aaGATGATGCCGACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCGATCAAAACg                 <  1:3036308/63‑1 (MQ=255)

                                              aaGATGATGCCGACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCGATCAAAACg                 <  1:1789447/63‑1 (MQ=255)

                                              aaGATGATGCCGACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCGATCAAAACg                 <  1:3197595/63‑1 (MQ=255)

                                              aaGATGATGCCGACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCGATCAAAACg                 <  1:1182170/63‑1 (MQ=255)

                                              aaGATGATGCCGACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCGATCAAAACg                 <  1:566603/63‑1 (MQ=255)

                                              aaGATGATGCCGACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCGATCAAAACg                 <  1:1079254/63‑1 (MQ=255)

                                              aaGATGATGCCGACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCGATCAAAACg                 <  1:1045552/63‑1 (MQ=255)

                                              aaGATGATGCCGACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCGATCAAAACg                 <  1:766707/63‑1 (MQ=255)

                                              aaGATGATGCCGACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCGATCAAAACg                 <  1:1785537/63‑1 (MQ=255)

                                              aaGATGATGCCGACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCGATCAAAACg                 <  1:2643713/63‑1 (MQ=255)

                                              aaGATGATGCCGACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCGATCAAAACg                 <  1:180851/63‑1 (MQ=255)

                                              aaGATGATGCCGACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCGATCAAAACg                 <  1:2605561/63‑1 (MQ=255)

                                              aaGATGATGCCGACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCGATCAAAACg                 <  1:2590122/63‑1 (MQ=255)

                                              aaGATGATGCCGACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCGATCAAAACg                 <  1:2453466/63‑1 (MQ=255)

                                              aaGATGATGCCGACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCGATCAAAACg                 <  1:1973274/63‑1 (MQ=255)

                                              aaGATGATGCCGACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCGATCAAAACg                 <  1:1291306/63‑1 (MQ=255)

                                              aaGATGATGCCAACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCGATCAAAACGCAAATc           >  1:2805997/1‑69 (MQ=255)

                                              aaGATGATGCCAACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCGATCAAAACGCAAATc           >  1:1050796/1‑69 (MQ=255)

                                              aaGATGATGCCAACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCGATCAAAACGCAAATc           >  1:489685/1‑69 (MQ=255)

                                              aaGATGATGCCAACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCGATCAAAACGCAAATc           >  1:447042/1‑69 (MQ=255)

                                              aaGATGATGCCAACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCGATCAAAACGCAAATc           >  1:424220/1‑69 (MQ=255)

                                              aaGATGATGCCAACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCGATCAAAACGCAAATc           >  1:3430242/1‑69 (MQ=255)

                                              aaGATGATGCCAACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCGATCAAAACGCAAATc           >  1:3235285/1‑69 (MQ=255)

                                              aaGATGATGCCAACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCGATCAAAACGCAAATc           >  1:136145/1‑69 (MQ=255)

                                              aaGATGATGCCAACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCGATCAAAACGCAAATc           >  1:1266432/1‑69 (MQ=255)

                                              aaGATGATGCCAACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCGATCAAAACGCAAATc           >  1:3081684/1‑69 (MQ=255)

                                              aaGATGATGCCAACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCGATCAAAACGCAAATc           >  1:3080231/1‑69 (MQ=255)

                                              aaGATGATGCCAACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCGATCAAAACGCAAATc           >  1:1259949/1‑69 (MQ=255)

                                              aaGATGATGCCAACAAACAGCGACATACGCGAATCTTCATTAAAGTACATGCCGATCa                      >  1:2913244/1‑58 (MQ=255)

                                                 atgatgCCAACAAACAGCGACATACGCTTATCTTCATTAAAGTACATGCCGATCAAAACGCAAATCAgg        <  1:1587065/69‑1 (MQ=255)

                                                 atgatgCCAACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCGATCAAAACGCAAATCAgg        <  1:2266265/69‑1 (MQ=255)

                                                 atgatgCCAACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCGATCAAAACGCAAATCAgg        <  1:850154/69‑1 (MQ=255)

                                                 atgatgCCAACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCGATCAAAACGCAAATCAgg        <  1:1739540/69‑1 (MQ=255)

                                                 atgatgCCAACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCGATCAAAACGCAAATCAgg        <  1:1074572/69‑1 (MQ=255)

                                                 atgatgCCAACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCGATCAAAACGCAAATCAgg        <  1:647289/69‑1 (MQ=255)

                                                 atgatgCCAACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCGATCAAAACGCAAATCAgg        <  1:61070/69‑1 (MQ=255)

                                                 atgatgCCAACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCGATCAAAACGCAAATCAgg        <  1:1665772/69‑1 (MQ=255)

                                                 atgatgCCAACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCGATCAAAACGCAAATCAgg        <  1:2986575/69‑1 (MQ=255)

                                                 atgatgCCAACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCGATCAAAACGCAAATCAgg        <  1:3432373/69‑1 (MQ=255)

                                                 atgatgCCAACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCGATCAAAACGCAAATCAgg        <  1:3302414/69‑1 (MQ=255)

                                                 atgatgCCAACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCGATCAAAACGCAAATCAgg        <  1:1654498/69‑1 (MQ=255)

                                                 atgatgCCAACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCGATCAAAACGCAAATCAgg        <  1:3114590/69‑1 (MQ=255)

                                                 atgatgCCAACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCGATCAAAACGCAAATCAgg        <  1:1527794/69‑1 (MQ=255)

                                                 atgatgCCAACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCGATCAAAACGCAAATCAgg        <  1:1496909/69‑1 (MQ=255)

                                                 atgatgCCAACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCGATCAAAACGCAAATCAgg        <  1:1216765/69‑1 (MQ=255)

                                                  tgatgCCAACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCGATCAAAACGCAAATCAgg        <  1:2929216/68‑1 (MQ=255)

                                                   gatgCCGACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCGATCAAAACg                 <  1:194919/58‑1 (MQ=255)

                                                      gCCAACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCGATCAAAACGCAAATCAGGAATGc   <  1:1879162/69‑1 (MQ=255)

                                                      gCCAACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCGATCAAAACGCAAATCAGGAATGc   <  1:1837761/69‑1 (MQ=255)

                                                       ccAACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCGATCAAAACGCAAATCAGGAATGcc  >  1:2558072/1‑69 (MQ=255)

GCGATTAAGGCCAAAAACTTTATAAATCGCCGTCACCGCCAGCATAAAGATGATGCCAACAAACAGCGACATACGCGTATCTTCATTAAAGTACATGCCGATCAAAACGCAAATCAGGAATGCC  >  minE/2416371‑2416494

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`