| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,417,935 | G→A | 35.2% | H241H (CAC→CAT) | rffM ← | UDP‑N‑acetyl‑D‑mannosaminuronic acid transferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,417,935 | 0 | G | A | 35.2% | 33.2 / 26.4 | 51 | H241H (CAC→CAT) | rffM | UDP‑N‑acetyl‑D‑mannosaminuronic acid transferase |
| Reads supporting (aligned to +/- strand): ref base G (30/3); new base A (12/6); total (42/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.20e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.10e-01 | |||||||||||
CAGACAGGCGACGGAGTGACCACTCCGTCGCTTTACAAAGAGAGGAAAATCATAGGTTGCCGGTGTAGTGCCAGCGTAAATAACGCAGCAAACGAAGCTGACGCTTAATGCGGCTCGGCTGCGAAAGCAGG > minE/2417868‑2417998 | cAGACAGGCGACGGAGTGACCTCTCCGTCGCTTTACAAAGAGAGGAAAATCATAGGTTGCCGGTGTAGt > 1:3156516/1‑69 (MQ=255)cAGACAGGCGACGGAGTGACCACTCCGTCGCTTTACAAAGAGAGGAAAATCATAGGTTGCCGGTGTAg > 1:3212101/1‑68 (MQ=255)cAGACAGGCGACGGAGTGACCACTCCGTCGCTTTACAAAGAGAGGAAAATCATAGGTTGCCGGTGTAGt > 1:3135647/1‑69 (MQ=255) ggAGTGACCACTCCGTCGCTTTACAAAGAGAGGAAAATCATAGGTTGCCGGTGTAGTGCCAGCGTAAAt > 1:2549279/1‑69 (MQ=255) ggAGTGACCACTCCGTCGCTTTACAAAGAGAGGAAAATCATAGGTTGCCGGTGTAGTGCCAGCGTAAAt > 1:2069203/1‑69 (MQ=255) ggAGTGACCACTCCGTCGCTTTACAAAGAGAGGAAAATCATAGGTTGCCGGTGTAGTGCCAGCGTAAAt > 1:52787/1‑69 (MQ=255) ggAGTGACCACTCCGTCGCTTTACAAAGAGAGGAAAATCATAGGTTGCCGGTGTAGTGCCAGCGTAAAt > 1:2747097/1‑69 (MQ=255) ggAGTGACCACTCCGTCGCTTTACAAAGAGAGGAAAATCATAGGTTGCCGGTGTAGTGCCAGCGTAAAt > 1:1965933/1‑69 (MQ=255) aCCACTCCGTCGCTTTACAAAGAGAGGAAAATCATAGGTTGCCGGTGTAGTGCCAGCGTAAATAACgc > 1:59971/1‑68 (MQ=255) aCCACTCCGTCGCTTTACAAAGAGAGGAAAATCATAGGTTGCCGGTGTAGTGCCAGCGTAAATAACgc > 1:1618617/1‑68 (MQ=255) ccACTCCGTCGCTTTACAAAGAGAGGAAAATCATAGGTTGCCGGTGTAGTGCCAGCGTAAATAACgca > 1:753011/1‑68 (MQ=255) ccACTCCGTCGCTTTACAAAGAGAGGAAAATCATAGGTTGCCGGTGTAGTGCCAGCGTAAATAACgca > 1:515936/1‑68 (MQ=255) ccACTCCGTCGCTTTACAAAGAGAGGAAAATCATAGGTTGCCGGTGTAGTGCCAGCGTAAATAACgca > 1:1677762/1‑68 (MQ=255) ccACTCCGTCGCTTTACAAAGAGAGGAAAATCATAGGTTGCCGGTGTAGTGCCAGCGTAAATAACgca > 1:1998000/1‑68 (MQ=255) cACTCCGTCGCTTTACAAAGAGAGGAAAATCATAGGTTGCCGGTGTAGTGCCAGCGTAAATAACgcag > 1:2218177/1‑68 (MQ=255) ccGTCGCTTTACAAAGAGAGGAAAATCATAGGTTGCCGGTGTAGt > 1:1207917/1‑45 (MQ=255) ccGTCGCTTTACAAAGAGAGGAAAATCATAGGTTGCCGGTGTAGTGCCAGCGTa > 1:2916927/1‑54 (MQ=255) ccGTCGCTTTACAAAGAGAGGAAAATCATAGGTTGCCGGTGTAGTGCCAGCGTa > 1:3389017/1‑54 (MQ=255) ccGTCGCTTTACAAAGAGAGGAAAATCATAGGTTGCCGGTGTAGTGCCAGCGTa > 1:933892/1‑54 (MQ=255) ccGTCGCTTTACAAAGAGAGGAAAATCATAGGTTGCCGGTGTAGTGCCAGCGTa > 1:2660724/1‑54 (MQ=255) ccGTCGCTTTACAAAGAGAGGAAAATCATAGGTTGCCGGTGTAGTGCCAGCGTa > 1:1521956/1‑54 (MQ=255) cGCTTTACAAAGAGAGGAAAATCATAGGTTGCCGGTGTAGTGCCAGCGTAAATAACGCAGCAAACGAAg < 1:2298369/69‑1 (MQ=255) cGCTTTACAAAGAGAGGAAAATCATAGGTTGCCGGTGTAGTGCCAGCGTAAATAACGCAGCAAACGAAg < 1:2010830/69‑1 (MQ=255) gCTTTACAAAGAGAGGAAAATCATAGGTTGCCGGTGTAGTGCCAGCGTAAATAACGCAGCAAACGAAg < 1:589842/68‑1 (MQ=255) cAAAGAGAGGAAAATCAGAGGTTGCCGGTGTAATGCCAGCGTAAATAACGCAGCAAACGAAGCTGAc < 1:974385/67‑1 (MQ=255) cAAAGAGAGGAAAATCAGAGGTTGCCGGTGTAATGCCAGCGTAAATAACGCAGCAAACGAAGCTGAc < 1:943031/67‑1 (MQ=255) cAAAGAGAGGAAAATCAGAGGTTGCCGGTGTAATGCCAGCGTAAATAACGCAGCAAACGAAGCTGAc < 1:1806321/67‑1 (MQ=255) cAAAGAGAGGAAAATCAGAGGTTGCCGGTGTAATGCCAGCGTAAATAACGCAGCAAACGAAGCTGAc < 1:2754115/67‑1 (MQ=255) cAAAGAGAGGAAAATCAGAGGTTGCCGGTGTAATGCCAGCGTAAATAACGCAGCAAACGAAGCTGAc < 1:2019878/67‑1 (MQ=255) aaaaTCATAGGTTGCCGGTGTAGTGCCAGCGTAAATAACGCAGCAAACGAAGCTGACGCTTAATGcggc > 1:26694/1‑69 (MQ=255) aaaaTCATAGGTTGCCGGTGTAGTGCCAGCGTAAATAACGCAGCAAACGAAGCTGACGCTTAATGcggc > 1:2251683/1‑69 (MQ=255) aaaaTCATAGGTTGCCGGTGTAGTGCCAGCGTAAATAACGCAGCAAACGAAGCTGACGCTTAATGcggc > 1:894094/1‑69 (MQ=255) aaaaTCATAGGTTGCCGGTGTAGTGCCAGCGTAAATAACGCAGCAAACGAAGCTGACGCTTAATGcggc > 1:793544/1‑69 (MQ=255) aaaaTCATAGGTTGCCGGTGTAGTGCCAGCGTAAATAACGCAGCAAACGAAGCTGACGCTTAATGcggc > 1:1347614/1‑69 (MQ=255) aaaaTCATAGGTTGCCGGTGTAGTGCCAGCGTAAATAACGCAGCAAACGAAGCTGACGCTTAATGcggc > 1:70037/1‑69 (MQ=255) aaaaTCATAGGTTGCCGGTGTAGTGCCAGCGTAAATAACGCAGCAAACGAAGCTGACGCTTAATGcggc > 1:1592821/1‑69 (MQ=255) aaaaTCATAGGTTGCCGGTGTAGTGCCAGCGTAAATAACGCAGCAAACGAAGCTGACGCTTAATGcggc > 1:1943476/1‑69 (MQ=255) aaaTCAGAGGTTGCCGGTGTAATGCCAGCGTAAATAACGCAGCAAACGAAGCTGAc < 1:501812/56‑1 (MQ=255) cATAGGTTGCCGGTGTAGTGCCAGCGTAAATAACGCAGCAAACGAAGCTGACGCTTAATGcggctcggc > 1:2176625/1‑69 (MQ=255) cATAGGTTGCCGGTGTAGTGCCAGCGTAAATAACGCAGCAAACGAAGCTGACGCTTAATGcggctcggc > 1:3125353/1‑69 (MQ=255) gtgtAATGCCAGCGTAAATAAGGCAGCAAACGAAGCTGACGCTTAATGCGGCTCGGCTGCGAAAGCAgg > 1:707335/1‑69 (MQ=255) gtgtAATGCCAGCGTAAATAACGCAGCAAACGAAGCTGACGCTTAATGCGGCTCGGCTGCGAAAGCAgg > 1:426663/1‑69 (MQ=255) gtgtAATGCCAGCGTAAATAACGCAGCAAACGAAGCTGACGCTTAATGCGGCTCGGCTGCGAAAGCAgg > 1:3301014/1‑69 (MQ=255) gtgtAATGCCAGCGTAAATAACGCAGCAAACGAAGCTGACGCTTAATGCGGCTCGGCTGCGAAAGCAgg > 1:2549192/1‑69 (MQ=255) gtgtAATGCCAGCGTAAATAACGCAGCAAACGAAGCTGACGCTTAATGCGGCTCGGCTGCGAAAGCAgg > 1:2521741/1‑69 (MQ=255) gtgtAATGCCAGCGTAAATAACGCAGCAAACGAAGCTGACGCTTAATGCGGCTCGGCTGCGAAAGCAgg > 1:1381560/1‑69 (MQ=255) gtgtAATGCCAGCGTAAATAACGCAGCAAACGAAGCTGACGCTTAATGCGGCTCGGCTGCGAAAGCAgg > 1:1932278/1‑69 (MQ=255) gtgtAATGCCAGCGTAAATAACGCAGCAAACGAAGCTGACGCTTAATGCGGCTCGGCTGCGAAAGCAgg > 1:1198591/1‑69 (MQ=255) gtgtAATGCCAGCGTAAATAACGCAGCAAACGAAGCTGACGCTTAATGCGGCTCGGCTGCGAAAGCAgg > 1:821455/1‑69 (MQ=255) gtgtAATGCCAGCGTAAATAACGCAGCAAACGAAGCTGACGCTTAATGCGGCTCGGCTGCGAAAGCAgg > 1:1223670/1‑69 (MQ=255) gtgtAATGCCAGCGTAAATAACGCAGCAAACGAAGCTGACGCTTAATGCGGCTCGGCTGCGAAAGCAgg > 1:2429694/1‑69 (MQ=255) gtgtAATGCCAGCGTAAATAACGCAGCAAACGAAGCTGACGCTTAATGCGGCTCGGCTGCGAAAGCAgg > 1:2017966/1‑69 (MQ=255) | CAGACAGGCGACGGAGTGACCACTCCGTCGCTTTACAAAGAGAGGAAAATCATAGGTTGCCGGTGTAGTGCCAGCGTAAATAACGCAGCAAACGAAGCTGACGCTTAATGCGGCTCGGCTGCGAAAGCAGG > minE/2417868‑2417998 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |