| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,418,505 | A→G | 22.0% | T51T (ACT→ACC) | rffM ← | UDP‑N‑acetyl‑D‑mannosaminuronic acid transferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,418,505 | 0 | A | G | 22.0% | 151.1 / 34.9 | 113 | T51T (ACT→ACC) | rffM | UDP‑N‑acetyl‑D‑mannosaminuronic acid transferase |
| Reads supporting (aligned to +/- strand): ref base A (39/49); new base G (2/23); total (41/72) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.34e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.07e-01 | |||||||||||
Only 100 of 115 total aligned reads displayed.
CCATCCGCATATTTAAATTCGGCAGCGTTAATTAACTCCCTGACCTCGGCGTTATCTTCAATAGTCAGCATTTTTTCAGCATTAATGGCAACCAGCGTTCCCTGCTTAAGCTGCCCGTCAGCAAACAGAT > minE/2418443‑2418572 | ccATCCGCATATTTAAATTCGGCAGCGTTAATTAACTCCCTGACCTCGGCGTTATCTTCAATGGTCAGc > 1:933894/1‑69 (MQ=255)ccATCCGCATATTTAAATTCGGCAGCGTTAATTAACTCCCTGACCTCGGCGTTATCTTCAATGGTCAGc > 1:663108/1‑69 (MQ=255) cATATTTAAATTCGTCAGCGTTAATTAACTCCCTGACCTCGGCGTTATCTTCAATGGTCAGCAttttt < 1:2986579/68‑1 (MQ=255) cATATTTAAATTCGTCAGCGTTAATTAACTCCCTGACCTCGGCGTTATCTTCAATGGTCAGCAttttt < 1:3005373/68‑1 (MQ=255) cATATTTAAATTCGGCAGCGTTAATTAAGTCCCTGACCTCGGCGTTATCTTCAATGGTCAGCAttttt < 1:567346/68‑1 (MQ=255) cATATTTAAATTCGGCAGCGTTAATTAACTCCCTGACCTCGGCGTTATCTTCAATGGTCAGCAttttt < 1:43349/68‑1 (MQ=255) cATATTTAAATTCGGCAGCGTTAATTAACTCCCTGACCTCGGCGTTATCTTCAATGGTCAGCAttttt < 1:3426823/68‑1 (MQ=255) cATATTTAAATTCGGCAGCGTTAATTAACTCCCTGACCTCGGCGTTATCTTCAATGGTCAGCAttttt < 1:926498/68‑1 (MQ=255) cATATTTAAATTCGGCAGCGTTAATTAACTCCCTGACCTCGGCGTTATCTTCAATGGTCAGCAttttt < 1:2246773/68‑1 (MQ=255) cATATTTAAATTCGGCAGCGTTAATTAACTCCCTGACCTCGGCGTTATCTTCAATGGTCAGCAttttt < 1:2284450/68‑1 (MQ=255) cATATTTAAATTCGGCAGCGTTAATTAACTCCCTGACCTCGGCGTTATCTTCAATGGTCAGCAttttt < 1:56586/68‑1 (MQ=255) cATATTTAAATTCGGCAGCGTTAATTAACTCCCTGACCTCGGCGTTATCTTCAATGGTCAGCAttttt < 1:2544717/68‑1 (MQ=255) cATATTTAAATTCGGCAGCGTTAATTAACTCCCTGACCTCGGCGTTATCTTCAATGGTCAGCAttttt < 1:2561353/68‑1 (MQ=255) cATATTTAAATTCGGCAGCGTTAATTAACTCCCTGACCTCGGCGTTATCTTCAATGGTCAGCAttttt < 1:2653229/68‑1 (MQ=255) cATATTTAAATTCGGCAGCGTTAATTAACTCCCTGACCTCGGCGTTATCTTCAATGGTCAGCAttttt < 1:1002172/68‑1 (MQ=255) cATATTTAAATTCGGCAGCGTTAATTAACTCCCTGACCTCGGCGTTATCTTCAATGGTCAGCAttttt < 1:2844879/68‑1 (MQ=255) cATATTTAAATTCGGCAGCGTTAATTAACTCCCTGACCTCGGCGTTATCTTCAATGGTCAGCAttttt < 1:96907/68‑1 (MQ=255) cATATTTAAATTCGGCAGCGTTAATTAACTCCCTGACCTCGGCGTTATCTTCAATGGTCAGCAttttt < 1:2988628/68‑1 (MQ=255) cATATTTAAATTCGGCAGCGTTAATTAACTCCCTGACCTCGGCGTTATCTTCAATGGTCAGCAttttt < 1:413997/68‑1 (MQ=255) cATATTTAAATTCGGCAGCGTTAATTAACTCCCTGACCTCGGCGTTATCTTCAATGGTCAGCAttttt < 1:3011884/68‑1 (MQ=255) cATATTTAAATTCGGCAGCGTTAATTAACTCCCTGACCTCGGCGTTATCTTCAATGGTCAGCAttttt < 1:3311586/68‑1 (MQ=255) cATATTTAAATTCGGCAGCGTTAATTAACTCCCTGACCTCGGCGTTATCTTCAATGGTCAGCAttttt < 1:1708929/68‑1 (MQ=255) cATATTTAAATTCGGCAGCGTTAATTAACTCCCTGACCTCGGCGTTATCTTCAATGGTCAGCAttttt < 1:96766/68‑1 (MQ=255) atatTTAAATTCGGCAGCGTTAATTAACTCCCTGACCTCGGCGTTATCTTCAATAGTCAGCATTTTTTc < 1:1778917/69‑1 (MQ=255) atatTTAAATTCGGCAGCGTTAATTAACTCCCTGACCTCGGCGTTATCTTCAATAGTCAGCATTTTTTc < 1:646633/69‑1 (MQ=255) atatTTAAATTCGGCAGCGTTAATTAACTCCCTGACCTCGGCGTTATCTTCAATAGTCAGCATTTTTTc < 1:59127/69‑1 (MQ=255) tatTTAAATTCGGCAGCGTTAATTAACTCCCTGACCTCGGCGTTATCTTCAATAGTCAGCATTTTTTc < 1:3296931/68‑1 (MQ=255) tttAAATTCGGCAGCGTTAATTAACTCCCTGACCTCGGCGTTATCTTCAATAGTCAGCATTTTTTCAGc > 1:2694618/1‑69 (MQ=255) tttAAATTCGGCAGCGTTAATTAACTCCCTGACCTCGGCGTTATCTTCAATAGTCAGCATTTTTTCAGc > 1:2765758/1‑69 (MQ=255) tttAAATTCGGCAGCGTTAATTAACTCCCTGACCTCGGCGTTATCTTCAATAGTCAGCATTTTTTCAGc > 1:490171/1‑69 (MQ=255) tttAAATTCGGCAGCGTTAATTAACTCCCTGACCTCGGCGTTATCTTCAATAGTCAGCATTTTTTCAGc > 1:2491635/1‑69 (MQ=255) tttAAATTCGGCAGCGTTAATTAACTCCCTGACCTCGGCGTTATCTTCAATAGTCAGCATTTTTTCAGc > 1:3101624/1‑69 (MQ=255) tttAAATTCGGCAGCGTTAATTAACTCCCTGACCTCGGCGTTATCTTCAATAGTCAGCATTTTTTCAGc > 1:1395011/1‑69 (MQ=255) tttAAATTCGGCAGCGTTAATTAACTCCCTGACCTCGGCGTTATCTTCAATAGTCAGCATTTTTTCAGc > 1:288325/1‑69 (MQ=255) gCAGCGTTAATTAACTCCCTGACCTCGGCGTTATCTTCAATAGTCAGCATTTTTTCAGCATTAATGGc < 1:121269/68‑1 (MQ=255) gCAGCGTTAATTAACTCCCTGACCTCGGCGTTATCTTCAATAGTCAGCATTTTTTCAGCATTAATGGc < 1:2841683/68‑1 (MQ=255) gCAGCGTTAATTAACTCCCTGACCTCGGCGTTATCTTCAATAGTCAGCATTTTTTCAGCATTAATGGc < 1:2896349/68‑1 (MQ=255) gCAGCGTTAATTAACTCCCTGACCTCGGCGTTATCTTCAATAGTCAGCATTTTTTCAGCATTAATGGc < 1:2975041/68‑1 (MQ=255) gCAGCGTTAATTAACTCCCTGACCTCGGCGTTATCTTCAATAGTCAGCATTTTTTCAGCATTAATGGc < 1:1342060/68‑1 (MQ=255) gCAGCGTTAATTAACTCCCTGACCTCGGCGTTATCTTCAATAGTCAGCATTTTTTCAGCATTAATGGc < 1:227515/68‑1 (MQ=255) cAGCGTTAATTAACTCCCTGACCTCGGCGTTATCTTCAATAGTCAGCATTTTTTCAGCATTAATGGCa < 1:3319420/68‑1 (MQ=255) cAGCGTTAATTAACTCCCTGACCTCGGCGTTATCTTCAATAGTCAGCATTTTTTCAGCATTAATGGCa < 1:2733181/68‑1 (MQ=255) cAGCGTTAATTAACTCCCTGACCTCGGCGTTATCTTCAATAGTCAGCATTTTTTCAGCATTAATGGCa < 1:1181173/68‑1 (MQ=255) cAGCGTTAATTAACTCCCTGACCTCGGCGTTATCTTCAATAGTCAGCATTTTTTCAGCATTAATGGCa < 1:1899119/68‑1 (MQ=255) cAGCGTTAATTAACTCCCTGACCTCGGCGTTATCTTCAATAGTCAGCATTTTTTCAGCATTAATGGCa < 1:2191724/68‑1 (MQ=255) cAGCGTTAATTAACTCCCTGACCTCGGCGTTATCTTCAATAGTCAGCATTTTTTCAGCATTAATGGCa < 1:2471198/68‑1 (MQ=255) ttaattaaCTCCCTGACCTCGGCGTTATCTTCAATAGTCAGCATTTTTTCAGCAtt < 1:1385670/56‑1 (MQ=255) ttaattaaCTCCCTGACCTCGGCGTTATCTTCAATAGTCAGCATTTTTTCAGCAtt < 1:1469917/56‑1 (MQ=255) ttaattaaCTCCCTGACCTCGGCGTTATCTTCAATAGTCAGCATTTTTTCAGCAtt < 1:1511542/56‑1 (MQ=255) ttaattaaCTCCCTGACCTCGGCGTTATCTTCAATAGTCAGCATTTTTTCAGCAtt < 1:1908096/56‑1 (MQ=255) ttaattaaCTCCCTGACCTCGGCGTTATCTTCAATAGTCAGCATTTTTTCAGCAtt < 1:522271/56‑1 (MQ=255) ttaattaaCTCCCTGACCTCGGCGTTATCTTCAATAGTCAGCATTTTTTCAGCAtt < 1:2040716/56‑1 (MQ=255) ttaattaaCTCCCTGACCTCGGCGTTATCTTCAATAGTCAGCATTTTTTCAGCAtt < 1:2388114/56‑1 (MQ=255) ttaattaaCTCCCTGACCTCGGCGTTATCTTCAATAGTCAGCATTTTTTCAGCAtt < 1:2785622/56‑1 (MQ=255) ttaattaaCTCCCTGACCTCGGCGTTATCTTCAATAGTCAGCATTTTTTCAGCAtt < 1:1418314/56‑1 (MQ=255) ttaattaaCTCCCTGACCTCGGCGTTATCTTCAATAGTCAGCATTTTTTCAGCAtt < 1:2148199/56‑1 (MQ=255) ttaattaaCTCCCTGACCTCGGCGTTATCTTCAATAGTCAGCATTTTTTCAGCAtt < 1:2879860/56‑1 (MQ=255) ttaattaaCTCCCTGACCTCGGCGTTATCTTCAATAGTCAGCATTTTTTCAGCAtt < 1:3320017/56‑1 (MQ=255) ttaattaaCTCCCTGACCTCGGCGTTATCTTCAATAGACAGCATTTTTTCAGCAtt < 1:3123700/56‑1 (MQ=255) aaCTCCCTGACCTCGGCGTTATCTTCAATAGTCAGCATTTTTTCAGCATTAATGGCAACCAGCGTTccc > 1:2628349/1‑69 (MQ=255) aaCTCCCTGACCTCGGCGTTATCTTCAATAGTCAGCATTTTTTCAGCATTAATGGCAACCAGCGTTccc > 1:2482469/1‑69 (MQ=255) tCCCTGACCTCGGCGTTATCTTCAATAGTCAGCATTTTTTCAGCATTAATGGCa > 1:3426755/1‑54 (MQ=255) tCCCTGACCTCGGCGTTATCTTCAATAGTCAGCATTTTTTCAGCATTAATGGCa > 1:1691635/1‑54 (MQ=255) tCCCTGACCTCGGCGTTATCTTCAATAGTCAGCATTTTTTCAGCATTAATGGCa > 1:1130526/1‑54 (MQ=255) tCCCTGACCTCGGCGTTATCTTCAATAGTCAGCATTTTTTCAGCATTAATGGCa > 1:2637847/1‑54 (MQ=255) tCCCTGACCTCGGCGTTATCTTCAATAGTCAGCATTTTTTCAGCATTAATGGCa > 1:1565646/1‑54 (MQ=255) tCCCTGACCTCGGCGTTATCTTCAATAGTCAGCATTTTTTCAGCATTAATGGCa > 1:13899/1‑54 (MQ=255) tCCCTGACCTCGGCGTTATCTTCAATAGTCAGCATTTTTTCAGCATTAATGGCa > 1:2698136/1‑54 (MQ=255) tCCCTGACCTCGGCGTTATCTTCAATAGTCAGCATTTTTTCAGCATTAATGGCa > 1:1518319/1‑54 (MQ=255) tCCCTGACCTCGGCGTTATCTTCAATAGTCAGCATTTTTTCAGCATTAATGGCa > 1:2957471/1‑54 (MQ=255) tCCCTGACCTCGGCGTTATCTTCAATAGTCAGCATTTTTTCAGCATTAATGGCa > 1:154445/1‑54 (MQ=255) tCCCTGACCTCGGCGTTATCTTCAATAGTCAGCATTTTTTCAGCATTAATGGCa > 1:2869216/1‑54 (MQ=255) tCCCTGACCTCGGCGTTATCTTCAATAGTCAGCATTTTTTCAGCATTAATGGCa > 1:596280/1‑54 (MQ=255) tCCCTGACCTCGGCGTTATCTTCAATAGTCAGAATTTTTTCAGCATTAATGGCa > 1:205888/1‑54 (MQ=255) cccTGACCTCGGCGTTATCTTCAATAGTCAGCATTTTTTCAGCATTAATGGc < 1:949367/52‑1 (MQ=255) cTCGGCGTTATCTTCAATAGTCAGCATTTTTTCAGCATTAATGGCAACCAGCGTTCCCTGCTTAAGCTg < 1:504886/69‑1 (MQ=255) cTCGGCGTTATCTTCAATAGTCAGCATTTTTTCAGCATTAATGGCAACCAGCGTTCCCTGCTTAAGCTg > 1:452390/1‑69 (MQ=255) cTCGGCGTTATCTTCAATAGTCAGCATTTTTTCAGCATTAATGGCAACCAGCGTTCCCTGCTTAAGCTg > 1:942750/1‑69 (MQ=255) cTCGGCGTTATCTTCAATAGTCAGCATTTTTTCAGCATTAATGGCAACCAGCGTTCCCTGCTTAAGCTg > 1:699633/1‑69 (MQ=255) tCGGCGTTATCTTCAATAGTCAGCATTTTTTCAGCATTAATGGCAACCAGCGTTCCCTGCTTAAGCTg < 1:65078/68‑1 (MQ=255) tCGGCGTTATCTTCAATAGTCAGCATTTTTTCAGCATTAATGGCAACCAGCGTTCCCTGCTTAAGCTg < 1:2286837/68‑1 (MQ=255) cAATAGTCAGCATTTTTTCAGCATTAATGGCAACCAGCGTTCCCTGCTTa < 1:2738205/50‑1 (MQ=255) cAATAGTCAGCATTTTTTCAGCATTAATGGCAACCAGCGTTCCCTGCTTa < 1:680236/50‑1 (MQ=255) cAATAGTCAGCATTTTTTCAGCATTAATGGCAACCAGCGTTCCCTGCTTa < 1:144009/50‑1 (MQ=255) cAATAGTCAGCATTTTTTCAGCATTAATGGCAACCAGCGTTCCCTGCTTa < 1:2627651/50‑1 (MQ=255) cAATAGTCAGCATTTTTTCAGCATTAATGGCAACCAGCGTTCCCTGCTTa < 1:2527041/50‑1 (MQ=255) cAATAGTCAGCATTTTTTCAGCATTAATGGCAACCAGCGTTCCCTGCTTa < 1:2470202/50‑1 (MQ=255) cAATAGTCAGCATTTTTTCAGCATTAATGGCAACCAGCGTTCCCTGCTTa < 1:179929/50‑1 (MQ=255) tAGTCAGCATTTTTTCAGCATTAATGGCAACCAGCGTTCCCTGCTTAAGCTGCCCGTCAGCAAACAGa < 1:3371381/68‑1 (MQ=255) tAGTCAGCATTTTTTCAGCATTAATGGCAACCAGCGTTCCCTGCTTAAGCTGCCCGTCAGCAAACAGa < 1:2253291/68‑1 (MQ=255) tAGTCAGCATTTTTTCAGCATTAATGGCAACCAGCGTTCCCTGCTTAAGCTGCCCGTCAGCAAACAGa < 1:2031578/68‑1 (MQ=255) tAGTCAGCATTTTTTCAGCATTAATGGCAACCAGCGTTCCCTGCTTAAGCTGCCCGTCAGCAAACAGAt > 1:1119612/1‑69 (MQ=255) tAGTCAGCATTTTTTCAGCATTAATGGCAACCAGCGTTCCCTGCTTAAGCTGCCCGTCAGCAAACAGAt > 1:474920/1‑69 (MQ=255) tAGTCAGCATTTTTTCAGCATTAATGGCAACCAGCGTTCCCTGCTTAAGCTGCCCGTCAGCAAACAGAt > 1:1137657/1‑69 (MQ=255) tAGTCAGCATTTTTTCAGCATTAATGGCAACCAGCGTTCCCTGCTTAAGCTGCCCGTCAGCAAACAGAt > 1:1252000/1‑69 (MQ=255) tAGTCAGCATTTTTTCAGCATTAATGGCAACCAGCGTTCCCTGCTTAAGCTGCCCGTCAGCAAACAGAt > 1:2387966/1‑69 (MQ=255) tAGTCAGCATTTTTTCAGCATTAATGGCAACCAGCGTTCCCTGCTTAAGCTGCCCGTCAGCAAACAGAt > 1:2755616/1‑69 (MQ=255) tAGTCAGCATTTTTTCAGCATTAATGGCAACCAGCGTTCCCTGCTTAAGCTGCCCGTCAGCAAACAGAt > 1:564525/1‑69 (MQ=255) aGTCAGCATTTTTTCAGCATTAATGGCAACCAGCGTTCCCTGCTTAAGCTGCCCGTCAGCAAACAGa < 1:3180696/67‑1 (MQ=255) aGTCAGCATTTTTTCAGCATTAATGGCAACCAGCGTTCCCTGCTTAAGCTGCCCGTCAGCAAACAGa < 1:431656/67‑1 (MQ=255) | CCATCCGCATATTTAAATTCGGCAGCGTTAATTAACTCCCTGACCTCGGCGTTATCTTCAATAGTCAGCATTTTTTCAGCATTAATGGCAACCAGCGTTCCCTGCTTAAGCTGCCCGTCAGCAAACAGAT > minE/2418443‑2418572 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |