| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,418,565 | A→G | 50.0% | F31F (TTT→TTC) | rffM ← | UDP‑N‑acetyl‑D‑mannosaminuronic acid transferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,418,565 | 0 | A | G | 50.0% | ‑3.8 / 66.7 | 74 | F31F (TTT→TTC) | rffM | UDP‑N‑acetyl‑D‑mannosaminuronic acid transferase |
| Reads supporting (aligned to +/- strand): ref base A (25/12); new base G (20/17); total (45/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.41e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.49e-01 | |||||||||||
TAGTCAGCATTTTTTCAGCATTAATGGCAACCAGCGTTCCCTGCTTAAGCTGCCCGTCAGCAAACAGATAATCGAGGGCGTGCTGCATATCACGCCAACCAATCAACTGTAAGCCACGCAGCGTA > minE/2418504‑2418628 | tAGTCAGCATTTTTTCAGCATTAATGGCAACCAGCGTTCCCTGCTTAAGCTGCCCGTCAGCAAACAGa < 1:2253291/68‑1 (MQ=255)tAGTCAGCATTTTTTCAGCATTAATGGCAACCAGCGTTCCCTGCTTAAGCTGCCCGTCAGCAAACAGa < 1:3415906/68‑1 (MQ=255)tAGTCAGCATTTTTTCAGCATTAATGGCAACCAGCGTTCCCTGCTTAAGCTGCCCGTCAGCAAACAGa > 1:999924/1‑68 (MQ=255)tAGTCAGCATTTTTTCAGCATTAATGGCAACCAGCGTTCCCTGCTTAAGCTGCCCGTCAGCAAACAGa < 1:3371381/68‑1 (MQ=255)tAGTCAGCATTTTTTCAGCATTAATGGCAACCAGCGTTCCCTGCTTAAGCTGCCCGTCAGCAAACAGa < 1:2031578/68‑1 (MQ=255)tAGTCAGCATTTTTTCAGCATTAATGGCAACCAGCGTTCCCTGCTTAAGCTGCCCGTCAGCAAACAGAt > 1:474920/1‑69 (MQ=255)tAGTCAGCATTTTTTCAGCATTAATGGCAACCAGCGTTCCCTGCTTAAGCTGCCCGTCAGCAAACAGAt > 1:1486916/1‑69 (MQ=255)tAGTCAGCATTTTTTCAGCATTAATGGCAACCAGCGTTCCCTGCTTAAGCTGCCCGTCAGCAAACAGAt > 1:564525/1‑69 (MQ=255)tAGTCAGCATTTTTTCAGCATTAATGGCAACCAGCGTTCCCTGCTTAAGCTGCCCGTCAGCAAACAGAt > 1:2755616/1‑69 (MQ=255)tAGTCAGCATTTTTTCAGCATTAATGGCAACCAGCGTTCCCTGCTTAAGCTGCCCGTCAGCAAACAGAt > 1:1252000/1‑69 (MQ=255)tAGTCAGCATTTTTTCAGCATTAATGGCAACCAGCGTTCCCTGCTTAAGCTGCCCGTCAGCAAACAGAt > 1:2387966/1‑69 (MQ=255)tAGTCAGCATTTTTTCAGCATTAATGGCAACCAGCGTTCCCTGCTTAAGCTGCCCGTCAGCAAACAGAt > 1:1137657/1‑69 (MQ=255)tAGTCAGCATTTTTTCAGCATTAATGGCAACCAGCGTTCCCTGCTTAAGCTGCCCGTCAGCAAACAGAt > 1:1119612/1‑69 (MQ=255) aGTCAGCATTTTTTCAGCATTAATGGCAACCAGCGTTCCCTGCTTAAGCTGCCCGTCAGCAAACAGa < 1:431656/67‑1 (MQ=255) aGTCAGCATTTTTTCAGCATTAATGGCAACCAGCGTTCCCTGCTTAAGCTGCCCGTCAGCAAACAGa < 1:3180696/67‑1 (MQ=255) gTCAGCATTTTTTCAGCATTAATGGCAACCAGCGTTCCCTGCTTAAGCTGCCCGTCAGCa < 1:2108566/60‑1 (MQ=255) gTCAGCATTTTTTCAGCATTAATGGCAACCAGCGTTCCCTGCTTAAGCTGCCCGTCAGCa < 1:2181808/60‑1 (MQ=255) gTCAGCATTTTTTCAGCATTAATGGCAACCAGCGTTCCCTGCTTAAGCTGCCCGTCAGCa < 1:3433388/60‑1 (MQ=255) gTCAGCATTTTTTCAGCATTAATGGCAACCAGCGTTCCCTGCTTAAGCTGCCCGTCAGCa < 1:1100669/60‑1 (MQ=255) gTCAGCATTTTTTCAGCATTAATGGCAACCAGCGTTCCCTGCTTAAGCTGCCCGTCAGCa < 1:1452171/60‑1 (MQ=255) gTCAGCATTTTTTCAGCATTAATGGCAACCAGCGTTCCCTGCTTAAGCTGCCCGTCAGCa < 1:2861989/60‑1 (MQ=255) gTCAGCATTTTTTCAGCATTAATGGCAACCAGCGTTCCCTGCTTAAGCTGCCCGTCAGCa < 1:1396472/60‑1 (MQ=255) aGCATTTTTTCAGCATTAATGGCAACCAGCGTTCCCTGCTTAAGCTGCCCGTCAGCAAACAGa < 1:3167988/63‑1 (MQ=255) cATTTTTTCGGCTTTAATGGCAACCAGCGTTCCCTGCTTAAGCTGCCCGTCAGCGAACAGATAATCGa < 1:1194556/68‑1 (MQ=255) cATTTTTTCGGCATTAATGGCAACCAGCGTTCCCTGCTTAAGCTGCTCGTCAGCGAACAGATAATCGa < 1:1525369/68‑1 (MQ=255) cATTTTTTCGGCATTAATGGCAACCAGCGTTCCCTGCTTAAGCTGCCCGTCAGCGAACAGATAATCGa < 1:2471559/68‑1 (MQ=255) cATTTTTTCGGCATTAATGGCAACCAGCGTTCCCTGCTTAAGCTGCCCGTCAGCGAACAGATAATCGa < 1:2262926/68‑1 (MQ=255) cATTTTTTCGGCATTAATGGCAACCAGCGTTCCCTGCTTAAGCTGCCCGTCAGCGAACAGATAATCGa < 1:3311040/68‑1 (MQ=255) cATTTTTTCGGCATTAATGGCAACCAGCGTTCCCTGCTTAAGCTGCCCGTCAGCGAACAGATAATCGa < 1:1117336/68‑1 (MQ=255) cATTTTTTCGGCATTAATGGCAACCAGCGTTCCCTGCTTAAGCTGCCCGTCAGCGAACAGATAATCGa < 1:982024/68‑1 (MQ=255) cATTTTTTCGGCATTAATGGCAACCAGCGTTCCCTGCTTAAGCTGCCCGTCAGCGAACAGATAATCGa < 1:931500/68‑1 (MQ=255) cATTTTTTCGGCATTAATGGCAACCAGCGTTCCCTGCTTAAGCTGCCCGTCAGCGAACAGATAATCGa < 1:837177/68‑1 (MQ=255) cATTTTTTCGGCATTAATGGCAACCAGCGTTCCCTGCTTAAGCTGCCCGTCAGCGAACAGATAATCGa < 1:1990799/68‑1 (MQ=255) cATTTTTTCGGCATTAATGGCAACCAGCGTTCCCTGCTTAAGCTGCCCGTCAGCGAACAGATAATCGa < 1:192060/68‑1 (MQ=255) cATTTTTTCGGCATTAATGGCAACCAGCGTTCCCTGCTTAAGCTGCCCGTCAGCGAACAGATAATCGa < 1:2883425/68‑1 (MQ=255) cATTTTTTCGGCATTAATGGCAACCAGCGTTCCCTGCTTAAGCTGCCCGTCAGCGAACAGATAATCGa < 1:1461487/68‑1 (MQ=255) cATTTTTTCGGCATTAATGGCAACCAGCGTTCCCTGCTTAAGCTGCCCGTCAGCGAACAGATAATCGa < 1:168006/68‑1 (MQ=255) aTTTTTTCGGCATTAATGGCAACCAGCGTTCCCTGCTTAAGCTGCCCGTCAGCGAACAGATAATCGa < 1:2755636/67‑1 (MQ=255) aTTTTTTCGGCATTAATGGCAACCAGCGTTCCCTGCTTAAGCTGCCCGTCAGCGAACAGATAATCGa < 1:1905300/67‑1 (MQ=255) cAGCATTAATGGCAACCAGCGTTCCCTGCTTAAGCTGCCCGTCAGCa < 1:1857253/47‑1 (MQ=255) ttAATGGCAACCAGCGTTCCCTGCTTAAGCTGCCCGTCAGCAAACAGATAATCGAGGGCGTGCTGCat < 1:605338/68‑1 (MQ=255) aaTGGCAACCAGCGTTCCCTGCTTAAGCTGCCCGTCAGCa < 1:3308797/40‑1 (MQ=255) aaTGGCAACCAGCGTTCCCTGCTTAAGCTGCCCGTCAGCa < 1:3355867/40‑1 (MQ=255) aaTGGCAACCAGCGTTCCCTGCTTAAGCTGCCCGTCAGCa < 1:3409518/40‑1 (MQ=255) aaTGGCAACCAGCGTTCCCTGCTTAAGCTGCCCGTCAGCa < 1:3413295/40‑1 (MQ=255) aaTGGCAACCAGCGTTCCCTGCTTAAGCTGCCCGTCAGCa < 1:3126288/40‑1 (MQ=255) aaTGGCAACCAGCGTTCCCTGCTTAAGCTGCCCGTCAGCa < 1:2999384/40‑1 (MQ=255) aaTGGCAACCAGCGTTCCCTGCTTAAGCTGCCCGTCAGCa < 1:649807/40‑1 (MQ=255) aaTGGCAACCAGCGTTCCCTGCTTAAGCTGCCCGTCAGCa < 1:1107329/40‑1 (MQ=255) aaTGGCAACCAGCGTTCCCTGCTTAAGCTGCCCGTCAGCa < 1:2187484/40‑1 (MQ=255) aaTGGCAACCAGCGTTCCCTGCTTAAGCTGCCCGTCAGCa < 1:1240957/40‑1 (MQ=255) aaTGGCAACCAGCGTTCCCTGCTTAAGCTGCCCGTCAGCa < 1:2382034/40‑1 (MQ=255) aaTGGCAACCAGCGTTCCCTGCTTAAGCTGCCCGTCAGCAATCAGATAATCGAGGGCGTGCTGCATATc > 1:1139179/1‑69 (MQ=255) aaTGGCAACCAGCGTTCCCTGCTTAAGCTGCCCGTCAGCAAACAGATAATCGAGGGCGt > 1:7176/1‑59 (MQ=255) aaTGGCAACCAGCGTTCCCTGCTTAAGCTGCCCGTCAGCAAACAGATAATCGAGGGCGt > 1:1866076/1‑59 (MQ=255) aaTGGCAACCAGCGTTCCCTGCTTAAGCTGCCCGTCAGCAAACAGATAATCGAGGGCGt > 1:1407153/1‑59 (MQ=255) aaTGGCAACCAGCGTTCCCTGCTTAAGCTGCCCGTCAGCAAACAGATAATCGAGGGCGt > 1:894390/1‑59 (MQ=255) aaTGGCAACCAGCGTTCCCTGCTTAAGCTGCCCGTCAGCAAACAGATAATCGAGGGCGt > 1:928711/1‑59 (MQ=255) aaTGGCAACCAGCGTTCCCTGCTTAAGCTGCCCGTCAGCAAACAGATAATCGAGGGCGTGCTGCatat > 1:659420/1‑68 (MQ=255) aaTGGCAACCAGCGTTCCCTGCTTAAGCTGCCCGTCAGCAAACAGATAATCGAGGGCGTGCTGCATATc > 1:1777372/1‑69 (MQ=255) aaTGGCAACCAGCGTTCCCTGCTTAAGCTGCCCGTCAGCAAACAGATAATCGAGGGCGTGCTGCATATc > 1:2349674/1‑69 (MQ=255) aaTGGCAACCAGCGTTCCCTGCTTAAGCTGCCCGTCAGCAAACAGATAATCGAGGGCGTGCTGCATATc > 1:2236567/1‑69 (MQ=255) aaTGGCAACCAGCGTTCCCTGCTTAAGCTGCCCGTCAGCAAACAGATAATCGAGGGCGTGCTGCATATc > 1:403860/1‑69 (MQ=255) aaTGGCAACCAGCGTTCCCTGCTTAAGCTGCCCGTCAGCAAACAGATAATCGAGGGCGTGCTGCATATc > 1:3320247/1‑69 (MQ=255) aaTGGCAACCAGCGTTCCCTGCTTAAGCTGCCCGTCAGCAAACAGATAATCGAGGGCGTGCTGCATATc > 1:3360875/1‑69 (MQ=255) aaTGGCAACCAGCGTTCCCTGCTTAAGCTGCCCGTCAGCAAACAGATAATCGAGGGCGTGCTGCATATc > 1:2998012/1‑69 (MQ=255) aaTGGCAACCAGCGTTCCCTGCTTAAGCTGCCCGTCAGCAAACAGATAATCGAGGGCGTGCTGCATATc > 1:266261/1‑69 (MQ=255) aaTGGCAACCAGCGTTCCCTGCTTAAGCAGCCCGTCAGCAAACAGATAATCGAGGGCGt > 1:1201702/1‑59 (MQ=255) aaCCAGCGTTCACTGCTTAAGCTGCCCGTCAGCAAACAGATAATCGAGGGCGTGCTGCATATCACGcca < 1:3004889/69‑1 (MQ=255) ccTGCTTAAGCTGACCGTCAGCGAACAGATAATCGa < 1:1919994/36‑1 (MQ=37) gCTGCCCGTCAGCGAACAGATAATCGAGGGCATGCTGCATATCACGCCAACCAATCAACTGTAAGCCa > 1:547206/1‑68 (MQ=255) gCTGCCCGTCAGCGAACAGATAATCGAGGGCATGCTGCATATCACGCCAACCAATCAACTGTAAGCCa > 1:549724/1‑68 (MQ=255) gCTGCCCGTCAGCGAACAGATAATCGAGGGCATGCTGCATATCACGCCAACCAATCAACTGTAAGCCa > 1:1408595/1‑68 (MQ=255) gCTGCCCGTCAGCGAACAGATAATCGAGGGCATGCTGCATATCACGCCAACCAATCAACTGTAAGCCa > 1:1344216/1‑68 (MQ=255) gCTGCCCGTCAGCGAACAGATAATCGAGGGCATGCTGCATATCACGCCAACCAATCAACTGTAAGCCa > 1:2028770/1‑68 (MQ=255) gCTGCCCGTCAGCGAACAGATAATCGAGGGCATGCTGCATATCACGCCAACCAATCAACTGTAAGCCa > 1:1233909/1‑68 (MQ=255) gCTGCCCGTCAGCGAACAGATAATCGAGGGCATGCTGCATATCACGCCAACCAATCAACTGTAAGCCa > 1:871707/1‑68 (MQ=255) gCTGCCCGTCAGCGAACAGATAATCGAGGGCATGCTGCATATCACGCCAACCAATCAACTGTAAGCCa > 1:1643450/1‑68 (MQ=255) gCTGCCCGTCAGCGAACAGATAATCGAGGGCATGCTGCATATCACGCCAACCAATCAACTGTAAGCCa > 1:2861585/1‑68 (MQ=255) gCTGCCCGTCAGCGAACAGATAATCGAGGGCATGCTGCATATCACGCCAACCAATCAACTGTAAGCCa > 1:2116992/1‑68 (MQ=255) gCTGCCCGTCAGCGAACAGATAATCGAGGGCATGCTGCATATCACGCCAACCAATCAACTGTAAGCCa > 1:3365236/1‑68 (MQ=255) gCTGCCCGTCAGCGAACAGATAATCGAGGGCATGCTGCATATCACGCCAACCAATCAACTGTAAGCCa > 1:2233342/1‑68 (MQ=255) gCTGCCCGTCAGCGAACAGATAATCGAGGGCATGCTGCATATCACGCCAACCAATCAACTGTAAGCCa > 1:3353058/1‑68 (MQ=255) gCTGCCCGTCAGCGAACAGATAATCGAGGGCATGCTGCATATCACGCCAACCAATCAACTGTAAGCCa > 1:3315657/1‑68 (MQ=255) gCTGCCCGTCAGCGAACAGATAATCGAGGGCATGCTGCATATCACGCCAACCAATCAACTGTAAGCCa > 1:3203072/1‑68 (MQ=255) gCTGCCCGTCAGCGAACAGATAATCGAGGGCATGCTGCATATCACGCCAACCAATCAACTGTAAGCCa > 1:2514188/1‑68 (MQ=255) gCTGCCCGTCAGCGAACAGATAATCGAGGGCATGCTGCATATCACGCCAACCAATCAACTGTAAGCCa > 1:3068562/1‑68 (MQ=255) gCTGCCCGTCAGCGAACAGATAATCGAGGGCATGCTGCATATCACGCCAACCAATCAACTGTAAGCCa > 1:2656064/1‑68 (MQ=255) gCTGCCCGTCAGCGAACAGATAATCGAGGGCATGCTGCATATCACGCCAACCAATCAACTGTAAGCCa > 1:2873701/1‑68 (MQ=255) gCTGCCCGTCAGCGAACAGATAATCGAGGGCATGCTGCATATCACGCCAACCAATCAACTGTAAGCCa > 1:2693614/1‑68 (MQ=255) tCAGCAAACAGATAATCGAGGGCGTGCTGCATATCACGCCAACCAATCAACTGTAAGCCACGCAGCGta < 1:654126/69‑1 (MQ=255) tCAGCAAACAGATAATCGAGGGCGTGCTGCATATCACGCCAACCAATCAACTGTAAGCCACGCAGCGta < 1:7555/69‑1 (MQ=255) tCAGCAAACAGATAATCGAGGGCGTGCTGCATATCACGCCAACCAATCAACTGTAAGCCACGCAGCGta < 1:2477772/69‑1 (MQ=255) | TAGTCAGCATTTTTTCAGCATTAATGGCAACCAGCGTTCCCTGCTTAAGCTGCCCGTCAGCAAACAGATAATCGAGGGCGTGCTGCATATCACGCCAACCAATCAACTGTAAGCCACGCAGCGTA > minE/2418504‑2418628 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |