| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,418,849 | A→G | 38.6% | F388F (TTT→TTC) | wzyE ← | predicted Wzy protein involved in ECA polysaccharide chain elongation |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,418,849 | 0 | A | G | 38.6% | 55.9 / 77.8 | 114 | F388F (TTT→TTC) | wzyE | predicted Wzy protein involved in ECA polysaccharide chain elongation |
| Reads supporting (aligned to +/- strand): ref base A (22/48); new base G (18/26); total (40/74) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.20e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01 | |||||||||||
Only 100 of 127 total aligned reads displayed.
AGACCACGCGTGAGACAAACGAATCCAGCCCTTCACGCGCCAGCACGATCATATTGAAGATCGCCCCAAAGCAGAAACTGTGCAATATCGCAGCTTTATAGCGATTAGGCTCGCGGTTGCCCAGCTCA > minE/2418782‑2418909 | aggccACGCGTGAGACAAACGAATCCAGCCCTTCACGCGCCAGCACGATCATATTGAAGATCGCCCCaa > 1:2455915/4‑69 (MQ=255)aGACCACGCGTGAGACAAACGAATCCAGCCCTTCACGCGCCAGCACGATCATATTGAAGATCGCCCCaa > 1:1004192/1‑69 (MQ=255)aGACCACGCGTGAGACAAACGAATCCAGCCCTTCACGCGCCAGCACGATCATATTGAAGATCGCCCCaa > 1:10279/1‑69 (MQ=255)aGACCACGCGTGAGACAAACGAATCCAGCCCTTCACGCGCCAGCACGATCATATTGAAGATCGCCCCaa > 1:858640/1‑69 (MQ=255)aGACCACGCGTGAGACAAACGAATCCAGCCCTTCACGCGCCAGCACGATCATATTGAAGATCGCCCCaa > 1:1094149/1‑69 (MQ=255)aGACCACGCGTGAGACAAACGAATCCAGCCCTTCACGCGCCAGCACGATCATATTGAAGATCGCCCCaa > 1:2201054/1‑69 (MQ=255)aGACCACGCGTGAGACAAACGAATCCAGCCCTTCACGCGCCAGCACGATCATATTGAAGATCGCCCCaa > 1:2292263/1‑69 (MQ=255)aGACCACGCGTGAGACAAACGAATCCAGCCCTTCACGCGCCAGCACGATCATATTGAAGATCGCCCCaa > 1:2952833/1‑69 (MQ=255)aGACCACGCGTGAGACAAACGAATCCAGCCCTTCACGCGCCAGCACGATCATATTGAAGATCGCCCCaa > 1:2912690/1‑69 (MQ=255)aGACCACGCGTGAGACAAACGAATCCAGCCCTTCACGCGCCAGCACGATCATATTGAAGATCGCCCCaa > 1:14508/1‑69 (MQ=255) gcgTGAGACAAACGAATCCATCCCTTCACGCGCCAGCACGATCATATTGAAGATCGCCCCGAAGCAGa < 1:2611573/68‑1 (MQ=255) gcgTGAGACAAACGAATCCAGCCCTTCACGCGCCAGCACGATCATATTGAAGATCGCCCCGAAGCAGa < 1:2083405/68‑1 (MQ=255) gcgTGAGACAAACGAATCCAGCCCTTCACGCGCCAGCACGATCATATTGAAGATCGCCCCGAAGCAGa < 1:211468/68‑1 (MQ=255) gcgTGAGACAAACGAATCCAGCCCTTCACGCGCCAGCACGATCATATTGAAGATCGCCCCGAAGCAGa < 1:2367072/68‑1 (MQ=255) gcgTGAGACAAACGAATCCAGCCCTTCACGCGCCAGCACGATCATATTGAAGATCGCCCCGAAGCAGa < 1:2498887/68‑1 (MQ=255) gcgTGAGACAAACGAATCCAGCCCTTCACGCGCCAGCACGATCATATTGAAGATCGCCCCGAAGCAGa < 1:2700946/68‑1 (MQ=255) gcgTGAGACAAACGAATCCAGCCCTTCACGCGCCAGCACGATCATATTGAAGATCGCCCCGAAGCAGa < 1:2758712/68‑1 (MQ=255) gcgTGAGACAAACGAATCCAGCCCTTCACGCGCCAGCACGATCATATTGAAGATCGCCCCGAAGCAGa < 1:298572/68‑1 (MQ=255) gcgTGAGACAAACGAATCCAGCCCTTCACGCGCCAGCACGATCATATTGAAGATCGCCCCGAAGCAGa < 1:300952/68‑1 (MQ=255) gcgTGAGACAAACGAATCCAGCCCTTCACGCGCCAGCACGATCATATTGAAGATCGCCCCGAAGCAGa < 1:331740/68‑1 (MQ=255) gcgTGAGACAAACGAATCCAGCCCTTCACGCGCCAGCACGATCATATTGAAGATCGCCCCGAAGCAGa < 1:3339499/68‑1 (MQ=255) gcgTGAGACAAACGAATCCAGCCCTTCACGCGCCAGCACGATCATATTGAAGATCGCCCCAAAGCAGaa < 1:252243/69‑1 (MQ=255) gcgTGAGACAAACGAATCCAGCCCTTCACGCGCCAGCACGATCATATTGAAGATCGCCCCAAAGCAGaa < 1:2891688/69‑1 (MQ=255) gcgTGAGACAAACGAATCCAGCCCTTCACGCGCCAGCACGATCATATTGAAGATCGCCCCAAAGCAGaa < 1:2484761/69‑1 (MQ=255) gcgTGAGACAAACGAATCCAGCCCTTCACGCGCCAGCACGATCATATTGAAGATCGCCCCAAAGCAGaa < 1:2911481/69‑1 (MQ=255) gcgTGAGACAAACGAATCCAGCCCTTCACGCGCCAGCACGATCATATTGAAGATCGCCCCAAAGCAGaa < 1:215203/69‑1 (MQ=255) gcgTGAGACAAACGAATCCAGCCCTTCACGCGCCAGCACGATCATATTGAAGATCGCCCCAAAGCAGaa < 1:970376/69‑1 (MQ=255) gcgTGAGACAAACGAATCCAGCCCTTCACGCGCCAGCACGATCATATTGAAGATCGCCACAAAGCAGaa < 1:816521/69‑1 (MQ=255) gcgTGAGACAAACGAATCCAGCCCTTCACGCGC‑‑‑CACGATCATATTGAAGATCGCCCCGAAGCAGa < 1:2587393/65‑1 (MQ=255) gTGAGACAAACGAATCCAGCCCTTCACGCGCCAGCACGATCATATTGAAGATCGCCCCGAAGCAGa < 1:1154592/66‑1 (MQ=255) gCCCTTCACGCGCCAGCACGATCATATTGAAGATCGCCCCAAAGCAGAAACTGTGCAATATCGCAGCtt < 1:2147073/69‑1 (MQ=255) ccTTCACGCGCCAGCACGATCATATTGAAGATCGCCCCAAAGCAGAAACTGTGCAATATCGCAGCtt > 1:1910767/1‑67 (MQ=255) ccTTCACGCGCCAGCACGATCATATTGAAGATCGCCCCAAAGCAGAAACTGTGCAATATCGCAGCtt > 1:705346/1‑67 (MQ=255) ccTTCACGCGCCAGCACGATCATATTGAAGATCGCCCCAAAGCAGAAACTGTGCAATATCGCAGCtt > 1:213412/1‑67 (MQ=255) cTTCACGCGCCAGCACGATCATATTGAAGATCGCCCCGAAGCAGa < 1:1598488/45‑1 (MQ=255) cACGCGCCAGCACGATCATATTGAAGATCGCCCCGAAGCAGa < 1:2380343/42‑1 (MQ=255) cACGCGCCAGCACGATCATATTGAAGATCGCCCCAAAGCAGa < 1:1649780/42‑1 (MQ=255) cACGCGCCAGCACGATCATATTGAAGATCGCCCCAAAGCAGAAACTGTGCAATATCGCAGCTTTATAGc > 1:578266/1‑69 (MQ=255) gcgcCAGCACGATCATATTGAAGATCGCCCCAAAGCAGAAACTGTGCAATATCGCAGCTTTATAGCGAt < 1:1823713/69‑1 (MQ=255) gcgcCAGCACGATCATATTGAAGATCGCCCCAAAGCAGAAACTGTGCAATATCGCAGCTTTATAGCGAt < 1:1713981/69‑1 (MQ=255) gcgcCAGCACGATCATATTGAAGATCGCCCCAAAGCAGAAACTGTGCAATATCGCAGCTTTATAGCGAt < 1:2482366/69‑1 (MQ=255) gcgcCAGCACGATCATATTGAAGATCGCCCCAAAGCAGAAACTGTGCAATATCGCAGCTTTATAGCGAt < 1:1030816/69‑1 (MQ=255) gcgcCAGCACGATCATATTGAAGATCGCCCCAAAGCAGAAACTGTGCAATATCGCAGCTTTATAGCGAt < 1:2369906/69‑1 (MQ=255) gcgcCAGCACGATCATATTGAAGATCGCCCCAAAGCAGAAACTGTGCAATATCGCAGCTTTATAGCGAt < 1:1560283/69‑1 (MQ=255) cAGCACGATCATATTGAAGATCGCCCCAAAGCAGAAACTGTGCAATATCGCAGCTTTATAGCGATTAgg < 1:2717515/69‑1 (MQ=255) cAGCACGATCATATTGAAGATCGCCCCAAAGCAGAAACTGTGCAATATCGCAGCTTTATAGCGATTAgg < 1:3324369/69‑1 (MQ=255) aGCACGATCATATTGAAGATCGCCCCAAAGCAGAAACTGTGCAATATCGCAGCTTTATAGCGATTAgg < 1:953096/68‑1 (MQ=255) aGCACGATCATATTGAAGATCGCCCCAAAGCAGAAACTGTGCAATATCGCAGCTTTATAGCGATTAGGc > 1:1217242/1‑69 (MQ=255) aGCACGATCATATTGAAGATCGCCCCAAAGCAGAAACTGTGCAATATCGCAGCTTTATAGCGATTAGGc > 1:2861628/1‑69 (MQ=255) cATATTGAAGATCGCCGCAAAGCAGAAACTGTGCAATATCGCAGCTTta < 1:31780/49‑1 (MQ=255) cATATTGAAGATCGCCCCAAAGCAGAAACTGTGCAATATCGCAGCTTta < 1:3139590/49‑1 (MQ=255) cATATTGAAGATCGCCCCAAAGCAGAAACTGTGCAATATCGCAGCTTta < 1:70118/49‑1 (MQ=255) cATATTGAAGATCGCCCCAAAGCAGAAACTGTGCAATATCGCAGCTTta < 1:1869662/49‑1 (MQ=255) cATATTGAAGATCGCCCCAAAGCAGAAACTGTGCAATATCGCAGCTTta < 1:3062788/49‑1 (MQ=255) cATATTGAAGATCGCCCCAAAGCAGAAACTGTGCAATATCGCAGCTTta < 1:1122923/49‑1 (MQ=255) cATATTGAAGATCGCCCCAAAGCAGAAACTGTGCAATATCGCAGCTTta < 1:2927222/49‑1 (MQ=255) cATATTGAAGATCGCCCCAAAGCAGAAACTGTGCAATATCGCAGCTTta < 1:3246833/49‑1 (MQ=255) cATATTGAAGATCGCCCCAAAGCAGAAACTGTGCAATATCGCAGCTTta < 1:1401118/49‑1 (MQ=255) cATATTGAAGATCGCCCCAAAGCAGAAACTGTGCAATATCGCAGCTTta < 1:1898364/49‑1 (MQ=255) cATATTGAAGATCGCCCCAAAGCAGAAACTGTGCAATATCGCAGCTTta < 1:2178073/49‑1 (MQ=255) cATATTGAAGATCGCCCCAAAGCAGAAACTGTGCAATATCGCAGCTTta < 1:2103309/49‑1 (MQ=255) cATATTGAAGATCGCCCCAAAGCAGAAACTGTGCAATATCGCAGCTTta < 1:1786531/49‑1 (MQ=255) cATATTGAAGATCGCCCCAAAGCAGAAACTGTGCAATATCGCAGCTTta < 1:2458322/49‑1 (MQ=255) atTGAAGATCGCCCCAAAGCAGAAACTGTGCAATATCGCAGCTTTATAGCGATTAGGCTCGCGGTTGc < 1:1370078/68‑1 (MQ=255) atTGAAGATCGCCCCAAAGCAGAAACTGTGCAATATCGCAGCTTTATAGCGATTAGGCTCGCGGTTGc < 1:1317808/68‑1 (MQ=255) atTGAAGATCGCCCCAAAGCAGAAACTGTGCAATATCGCAGCTTTATAGCGATTAGGCTCGCGGTTGc < 1:694908/68‑1 (MQ=255) atTGAAGATCGCCCCAAAGCAGAAACTGTGCAATATCGCAGCTTTATAGCGATTAGGCTCGCGGTTGc < 1:1186567/68‑1 (MQ=255) ttGAAGATCGCCCCAAAGCAGAAACTGTGCAATATCGCAGCTTTATAg > 1:1499886/1‑48 (MQ=255) ttGAAGATCGCCCCAAAGCAGAAACTGTGCAATATCGCAGCTTTATAGc > 1:1058103/1‑49 (MQ=255) ttGAAGATCGCCCCAAAGCAGAAACTGTGCAATATCGCAGCTTTATAGc > 1:1678668/1‑49 (MQ=255) ttGAAGATCGCCCCAAAGCAGAAACTGTGCAATATCGCAGCTTTATAGc > 1:556194/1‑49 (MQ=255) ttGAAGATCGCCCCAAAGCAGAAACTGTGCAATATCGCAGCTTTATAGc > 1:2198559/1‑49 (MQ=255) ttGAAGATCGCCCCAAAGCAGAAACTGTGCAATATCGCAGCTTTATAGc > 1:2328238/1‑49 (MQ=255) ttGAAGATCGCCCCAAAGCAGAAACTGTGCAATATCGCAGCTTTATAGc > 1:728818/1‑49 (MQ=255) ttGAAGATCGCCCCAAAGCAGAAACTGTGCAATATCGCAGCTTTATAGc > 1:832739/1‑49 (MQ=255) ttGAAGATCGCCCCAAAGCAGAAACTGTGCAATATCGCAGCTTTATAGc > 1:1914798/1‑49 (MQ=255) ttGAAGATCGCCCCAAAGCAGAAACTGTGCAATATCGCAGCTTTATAGc > 1:913718/1‑49 (MQ=255) ttGAAGATCGCCCCAAAGCAGAAACTGTGCAATATCGCAGCTTTATAGc > 1:1431290/1‑49 (MQ=255) tGAAGATCGCCCCGAAGCAGAAACTGTGCAATATCGCAGCCTTATAGCGATTAGTCTcg > 1:1462487/1‑59 (MQ=255) tGAAGATCGCCCCGAAGCAGAAACTGTGCAATATCGCAGCCTTATAGCGATTAGTCTCGCGGTTGccc > 1:202159/1‑68 (MQ=255) tGAAGATCGCCCCGAAGCAGAAACTGTGCAATATCGCAGCCTTATAGCGATTAGTCTCGCGGTTGccc > 1:1223014/1‑68 (MQ=255) tGAAGATCGCCCCGAAGCAGAAACTGTGCAATATCGCAGCCTTATAGCGATTAGTCTCGCGGTTGccc > 1:2671785/1‑68 (MQ=255) tGAAGATCGCCCCGAAGCAGAAACTGTGCAATATCGCAGCCTTATAGCGATTAGTCTCGCGGTTGccc > 1:541787/1‑68 (MQ=255) aGATCGCCCCGAAGCAGAAACTGTGCAATATCGCAGCCTTATAGCGATTAGTCTCGCGGTTGCCCAGc < 1:840325/68‑1 (MQ=255) aGATCGCCCCGAAGCAGAAACTGTGCAATATCGCAGCCTTATAGCGATTAGTCTCGCGGTTGCCCAGc < 1:101314/68‑1 (MQ=255) aGATCGCCCCGAAGCAGAAACTGTGCAATATCGCAGCCTTATAGCGATTAGTCTCGCGGTTGCCCAGc < 1:3149250/68‑1 (MQ=255) aGATCGCCCCGAAGCAGAAACTGTGCAATATCGCAGCCTTATAGCGATTAGTCTCGCGGTTGCCCAGc < 1:2378216/68‑1 (MQ=255) aGATCGCCCCGAAGCAGAAACTGTGCAATATCGCAGCCTTATAGCGATTAGTCTCGCGGTTGCCCAGc < 1:2498539/68‑1 (MQ=255) aGATCGCCCCGAAGCAGAAACTGTGCAATATCGCAGCCTTATAGCGATTAGTCTCGCGGTTGCCCAGc < 1:2672245/68‑1 (MQ=255) aTCGCCCCAAAGCAGAAACTGTGCAATATCGCAGCTTta < 1:3023527/39‑1 (MQ=255) aTCGCCCCAAAGCAGAAACTGTGCAATATCGCAGCTTTATAGCGATTAGGCTCGCGGTTGCCCAGCTCa < 1:3255101/69‑1 (MQ=255) aTCGCCCCAAAGCAGAAACTGTGCAATATCGCAGCTTTATAGCGATTAGGCTCGCGGTTGCCCAGCTCa < 1:244504/69‑1 (MQ=255) aTCGCCCCAAAGCAGAAACTGTGCAATATCGCAGCTTTATAGCGATTAGGCTCGCGGTTGCCCAGCTCa < 1:2587716/69‑1 (MQ=255) cGCCCCGAAGCAGAAACTGTGCAATATCGCAGCCTTATAGCGATTAGTCTCGCGGTTGCCCAGCTCa > 1:352404/1‑67 (MQ=255) cGCCCCGAAGCAGAAACTGTGCAATATCGCAGCCTTATAGCGATTAGTCTCGCGGTTGCCCAGCTCa > 1:1103389/1‑67 (MQ=255) cGCCCCGAAGCAGAAACTGTGCAATATCGCAGCCTTATAGCGATTAGTCTCGCGGTTGCCCAGCTCa > 1:3243062/1‑67 (MQ=255) cGCCCCGAAGCAGAAACTGTGCAATATCGCAGCCTTATAGCGATTAGTCTCGCGGTTGCCCAGCTCa > 1:320916/1‑67 (MQ=255) cGCCCCGAAGCAGAAACTGTGCAATATCGCAGCCTTATAGCGATTAGTCTCGCGGTTGCCCAGCTCa > 1:1921868/1‑67 (MQ=255) cGCCCCGAAGCAGAAACTGTGCAATATCGCAGCCTTATAGCGATTAGTCTCGCGGTTGCCCAGCTCa > 1:1258592/1‑67 (MQ=255) cGCCCCGAAGCAGAAACTGTGAAATATCGCAGCCTTATAGCGATTAGTCTCGCGGTTGCCCAGCTCa > 1:1473443/1‑67 (MQ=255) | AGACCACGCGTGAGACAAACGAATCCAGCCCTTCACGCGCCAGCACGATCATATTGAAGATCGCCCCAAAGCAGAAACTGTGCAATATCGCAGCTTTATAGCGATTAGGCTCGCGGTTGCCCAGCTCA > minE/2418782‑2418909 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |