| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,418,876 | T→C | 24.2% | K379K (AAA→AAG) | wzyE ← | predicted Wzy protein involved in ECA polysaccharide chain elongation |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,418,876 | 0 | T | C | 24.2% | 132.0 / 37.7 | 107 | K379K (AAA→AAG) | wzyE | predicted Wzy protein involved in ECA polysaccharide chain elongation |
| Reads supporting (aligned to +/- strand): ref base T (37/44); new base C (18/8); total (55/52) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.40e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.59e-01 | |||||||||||
Only 100 of 111 total aligned reads displayed.
GCCCTTCACGCGCCAGCACGATCATATTGAAGATCGCCCCAAAGCAGAAACTGTGCAATATCGCAGCTTTATAGCGATTAGGCTCGCGGTTGCCCAGCTCATACAGCCAGTCGAACCATTTGATGATCAGACCA > minE/2418809‑2418942 | gCCCTTCACGCGCCAGCACGATCATATTGAAGATCGCCCCAAAGCAGAAACTGTGCAATATCGCAGCtt < 1:2147073/69‑1 (MQ=255) ccTTCACGCGCCAGCACGATCATATTGAAGATCGCCCCAAAGCAGAAACTGTGCAATATCGCAGCtt > 1:705346/1‑67 (MQ=255) ccTTCACGCGCCAGCACGATCATATTGAAGATCGCCCCAAAGCAGAAACTGTGCAATATCGCAGCtt > 1:213412/1‑67 (MQ=255) ccTTCACGCGCCAGCACGATCATATTGAAGATCGCCCCAAAGCAGAAACTGTGCAATATCGCAGCtt > 1:1910767/1‑67 (MQ=255) cACGCGCCAGCACGATCATATTGAAGATCGCCCCAAAGCAGAAACTGTGCAATATCGCAGCTTTATAGc > 1:578266/1‑69 (MQ=255) cACGCGCCAGCACGATCATATTGAAGATCGCCCCAAAGCAGAAACTGTGCAATATCGCAGCTTTATAGc > 1:53123/1‑69 (MQ=255) gcgcCAGCACGATCATATTGAAGATCGCCCCAAAGCAGAAACTGTGCAATATCGCAGCTTTATAGCGAt < 1:1030816/69‑1 (MQ=255) gcgcCAGCACGATCATATTGAAGATCGCCCCAAAGCAGAAACTGTGCAATATCGCAGCTTTATAGCGAt < 1:1713981/69‑1 (MQ=255) gcgcCAGCACGATCATATTGAAGATCGCCCCAAAGCAGAAACTGTGCAATATCGCAGCTTTATAGCGAt < 1:2963127/69‑1 (MQ=255) gcgcCAGCACGATCATATTGAAGATCGCCCCAAAGCAGAAACTGTGCAATATCGCAGCTTTATAGCGAt < 1:1823713/69‑1 (MQ=255) gcgcCAGCACGATCATATTGAAGATCGCCCCAAAGCAGAAACTGTGCAATATCGCAGCTTTATAGCGAt < 1:1560283/69‑1 (MQ=255) gcgcCAGCACGATCATATTGAAGATCGCCCCAAAGCAGAAACTGTGCAATATCGCAGCTTTATAGCGAt < 1:2482366/69‑1 (MQ=255) gcgcCAGCACGATCATATTGAAGATCGCCCCAAAGCAGAAACTGTGCAATATCGCAGCTTTATAGCGAt < 1:124713/69‑1 (MQ=255) gcgcCAGCACGATCATATTGAAGATCGCCCCAAAGCAGAAACTGTGCAATATCGCAGCTTTATAGCGAt < 1:2369906/69‑1 (MQ=255) cAGCACGATCATATTGAAGATCGCCCCAAAGCAGAAACTGTGCAATATCGCAGCTTTATAGCGATTAgg < 1:3324369/69‑1 (MQ=255) cAGCACGATCATATTGAAGATCGCCCCAAAGCAGAAACTGTGCAATATCGCAGCTTTATAGCGATTAgg < 1:2432164/69‑1 (MQ=255) cAGCACGATCATATTGAAGATCGCCCCAAAGCAGAAACTGTGCAATATCGCAGCTTTATAGCGATTAgg < 1:2717515/69‑1 (MQ=255) aGCACGATCATATTGAAGATCGCCCCAAAGCAGAAACTGTGCAATATCGCAGCTTTATAGCGATTAGGc > 1:2460556/1‑69 (MQ=255) aGCACGATCATATTGAAGATCGCCCCAAAGCAGAAACTGTGCAATATCGCAGCTTTATAGCGATTAGGc > 1:2861628/1‑69 (MQ=255) cATATTGAAGATCGCCGCAAAGCAGAAACTGTGCAATATCGCAGCTTta < 1:31780/49‑1 (MQ=255) cATATTGAAGATCGCCCCAAAGCAGAAACTGTGCAATATCGCAGCTTta < 1:2458322/49‑1 (MQ=255) cATATTGAAGATCGCCCCAAAGCAGAAACTGTGCAATATCGCAGCTTta < 1:1898364/49‑1 (MQ=255) cATATTGAAGATCGCCCCAAAGCAGAAACTGTGCAATATCGCAGCTTta < 1:1869662/49‑1 (MQ=255) cATATTGAAGATCGCCCCAAAGCAGAAACTGTGCAATATCGCAGCTTta < 1:2103309/49‑1 (MQ=255) cATATTGAAGATCGCCCCAAAGCAGAAACTGTGCAATATCGCAGCTTta < 1:2178073/49‑1 (MQ=255) cATATTGAAGATCGCCCCAAAGCAGAAACTGTGCAATATCGCAGCTTta < 1:3062788/49‑1 (MQ=255) cATATTGAAGATCGCCCCAAAGCAGAAACTGTGCAATATCGCAGCTTta < 1:2927222/49‑1 (MQ=255) cATATTGAAGATCGCCCCAAAGCAGAAACTGTGCAATATCGCAGCTTta < 1:1786531/49‑1 (MQ=255) cATATTGAAGATCGCCCCAAAGCAGAAACTGTGCAATATCGCAGCTTta < 1:3246833/49‑1 (MQ=255) cATATTGAAGATCGCCCCAAAGCAGAAACTGTGCAATATCGCAGCTTta < 1:1401118/49‑1 (MQ=255) cATATTGAAGATCGCCCCAAAGCAGAAACTGTGCAATATCGCAGCTTta < 1:1137647/49‑1 (MQ=255) cATATTGAAGATCGCCCCAAAGCAGAAACTGTGCAATATCGCAGCTTta < 1:1122923/49‑1 (MQ=255) atTGAAGATCGCCCCAAAGCAGAAACTGTGCAATATCGCAGCTTTATAGCGATTAGGCTCGCGGTTGc < 1:1370078/68‑1 (MQ=255) atTGAAGATCGCCCCAAAGCAGAAACTGTGCAATATCGCAGCTTTATAGCGATTAGGCTCGCGGTTGc < 1:1317808/68‑1 (MQ=255) atTGAAGATCGCCCCAAAGCAGAAACTGTGCAATATCGCAGCTTTATAGCGATTAGGCTCGCGGTTGc < 1:1186567/68‑1 (MQ=255) atTGAAGATCGCCCCAAAGCAGAAACTGTGCAATATCGCAGCTTTATAGCGATTAGGCTCGCGGTTGc < 1:694908/68‑1 (MQ=255) ttGAAGATCGCCCCAAAGCAGAAACTGTGCAATATCGCAGCTTTATAg > 1:1499886/1‑48 (MQ=255) ttGAAGATCGCCCCAAAGCAGAAACTGTGCAATATCGCAGCTTTATAGc > 1:2328238/1‑49 (MQ=255) ttGAAGATCGCCCCAAAGCAGAAACTGTGCAATATCGCAGCTTTATAGc > 1:832739/1‑49 (MQ=255) ttGAAGATCGCCCCAAAGCAGAAACTGTGCAATATCGCAGCTTTATAGc > 1:2359501/1‑49 (MQ=255) ttGAAGATCGCCCCAAAGCAGAAACTGTGCAATATCGCAGCTTTATAGc > 1:728818/1‑49 (MQ=255) ttGAAGATCGCCCCAAAGCAGAAACTGTGCAATATCGCAGCTTTATAGc > 1:556194/1‑49 (MQ=255) ttGAAGATCGCCCCAAAGCAGAAACTGTGCAATATCGCAGCTTTATAGc > 1:3197775/1‑49 (MQ=255) ttGAAGATCGCCCCAAAGCAGAAACTGTGCAATATCGCAGCTTTATAGc > 1:913718/1‑49 (MQ=255) ttGAAGATCGCCCCAAAGCAGAAACTGTGCAATATCGCAGCTTTATAGc > 1:2198559/1‑49 (MQ=255) ttGAAGATCGCCCCAAAGCAGAAACTGTGCAATATCGCAGCTTTATAGc > 1:1058103/1‑49 (MQ=255) ttGAAGATCGCCCCAAAGCAGAAACTGTGCAATATCGCAGCTTTATAGc > 1:1914798/1‑49 (MQ=255) ttGAAGATCGCCCCAAAGCAGAAACTGTGCAATATCGCAGCTTTATAGc > 1:1431290/1‑49 (MQ=255) ttGAAGATCGCCCCAAAGCAGAAACTGTGCAATATCGCAGCTTTATAGc > 1:1603950/1‑49 (MQ=255) ttGAAGATCGCCCCAAAGCAGAAACTGTGCAATATCGCAGCTTTATAGc > 1:1678668/1‑49 (MQ=255) tGAAGATCGCCCCGAAGCAGAAACTGTGCAATATCGCAGCCTTATAGCGATTAGTCTcg > 1:1462487/1‑59 (MQ=255) tGAAGATCGCCCCGAAGCAGAAACTGTGCAATATCGCAGCCTTATAGCGATTAGTCTCGCGGTTGccc > 1:896083/1‑68 (MQ=255) tGAAGATCGCCCCGAAGCAGAAACTGTGCAATATCGCAGCCTTATAGCGATTAGTCTCGCGGTTGccc > 1:1223014/1‑68 (MQ=255) tGAAGATCGCCCCGAAGCAGAAACTGTGCAATATCGCAGCCTTATAGCGATTAGTCTCGCGGTTGccc > 1:541787/1‑68 (MQ=255) tGAAGATCGCCCCGAAGCAGAAACTGTGCAATATCGCAGCCTTATAGCGATTAGTCTCGCGGTTGccc > 1:2671785/1‑68 (MQ=255) aGATCGCCCCGAAGCAGAAACTGTGCAATATCGCAGCCTTATAGCGATTAGTCTCGCGGTTGCCCAGc < 1:101314/68‑1 (MQ=255) aGATCGCCCCGAAGCAGAAACTGTGCAATATCGCAGCCTTATAGCGATTAGTCTCGCGGTTGCCCAGc < 1:840325/68‑1 (MQ=255) aGATCGCCCCGAAGCAGAAACTGTGCAATATCGCAGCCTTATAGCGATTAGTCTCGCGGTTGCCCAGc < 1:3149250/68‑1 (MQ=255) aGATCGCCCCGAAGCAGAAACTGTGCAATATCGCAGCCTTATAGCGATTAGTCTCGCGGTTGCCCAGc < 1:2701189/68‑1 (MQ=255) aGATCGCCCCGAAGCAGAAACTGTGCAATATCGCAGCCTTATAGCGATTAGTCTCGCGGTTGCCCAGc < 1:2378216/68‑1 (MQ=255) aGATCGCCCCGAAGCAGAAACTGTGCAATATCGCAGCCTTATAGCGATTAGTCTCGCGGTTGCCCAGc < 1:2672245/68‑1 (MQ=255) aGATCGCCCCGAAGCAGAAACTGTGCAATATCGCAGCCTTATAGCGATTAGTCTCGCGGTTGCCCAGc < 1:2498539/68‑1 (MQ=255) aTCGCCCCAAAGCAGAAACTGTGCAATATCGCAGCTTta < 1:3023527/39‑1 (MQ=255) aTCGCCCCAAAGCAGAAACTGTGCAATATCGCAGCTTTATAGCGATTAGGCTCGCGGTTGCCCAGCTCa < 1:244504/69‑1 (MQ=255) aTCGCCCCAAAGCAGAAACTGTGCAATATCGCAGCTTTATAGCGATTAGGCTCGCGGTTGCCCAGCTCa < 1:1036896/69‑1 (MQ=255) aTCGCCCCAAAGCAGAAACTGTGCAATATCGCAGCTTTATAGCGATTAGGCTCGCGGTTGCCCAGCTCa < 1:3255101/69‑1 (MQ=255) aTCGCCCCAAAGCAGAAACTGTGCAATATCGCAGCTTTATAGCGATTAGGCTCGCGGTTGCCCAGCTCa < 1:2587716/69‑1 (MQ=255) cGCCCCGAAGCAGAAACTGTGCAATATCGCAGCCTTATAGCGATTAGTCTCGCGGTTGCCCAGCTCa > 1:320916/1‑67 (MQ=255) cGCCCCGAAGCAGAAACTGTGCAATATCGCAGCCTTATAGCGATTAGTCTCGCGGTTGCCCAGCTCa > 1:1103389/1‑67 (MQ=255) cGCCCCGAAGCAGAAACTGTGCAATATCGCAGCCTTATAGCGATTAGTCTCGCGGTTGCCCAGCTCa > 1:1921868/1‑67 (MQ=255) cGCCCCGAAGCAGAAACTGTGCAATATCGCAGCCTTATAGCGATTAGTCTCGCGGTTGCCCAGCTCa > 1:1258592/1‑67 (MQ=255) cGCCCCGAAGCAGAAACTGTGCAATATCGCAGCCTTATAGCGATTAGTCTCGCGGTTGCCCAGCTCa > 1:352404/1‑67 (MQ=255) cGCCCCGAAGCAGAAACTGTGCAATATCGCAGCCTTATAGCGATTAGTCTCGCGGTTGCCCAGCTCa > 1:1898872/1‑67 (MQ=255) cGCCCCGAAGCAGAAACTGTGCAATATCGCAGCCTTATAGCGATTAGTCTCGCGGTTGCCCAGCTCa > 1:769780/1‑67 (MQ=255) cGCCCCGAAGCAGAAACTGTGCAATATCGCAGCCTTATAGCGATTAGTCTCGCGGTTGCCCAGCTCa > 1:3243062/1‑67 (MQ=255) cGCCCCGAAGCAGAAACTGTGCAATATCGCAGCCTTATAGCGATTAGTCTCGCGGTTGCCCAGCTCa > 1:3073821/1‑67 (MQ=255) cGCCCCGAAGCAGAAACTGTGCAATATCGCAGCCTTATAGCGATTAGTCTCGCGGTTGCCCAGCTCa > 1:1463813/1‑67 (MQ=255) cAGAAACTGTGCAATATCGCAGCTTTATAGCGAtt > 1:111030/1‑35 (MQ=255) cAGAAACTGTGCAATATCGCAGCTTTATAGCGATTa < 1:371811/36‑1 (MQ=255) cAGAAACTGTGCAATATCGCAGCTTTATAGCGATTa > 1:893014/1‑36 (MQ=255) cAGAAACTGTGCAATATCGCAGCTTTATAGCGATTa > 1:1791135/1‑36 (MQ=255) cAGAAACTGTGCAATATCGCAGCTTTATAGCGATTa < 1:2873551/36‑1 (MQ=255) cAGAAACTGTGCAATATCGCAGCTTTATAGCGATTa > 1:1741160/1‑36 (MQ=255) cAGAAACTGTGCAATATCGCAGCTTTATAGCGATTa < 1:2930695/36‑1 (MQ=255) cAGAAACTGTGCAATATCGCAGCTTTATAGCGATTa > 1:3031214/1‑36 (MQ=255) cAGAAACTGTGCAATATCGCAGCTTTATAGCGATTa > 1:162790/1‑36 (MQ=255) cAGAAACTGTGCAATATCGCAGCTTTATAGCGATTa < 1:3138830/36‑1 (MQ=255) aGAAACTGTGCAATATCGCAGCTTTATAGCGATTa < 1:779266/35‑1 (MQ=255) tCGCAGCTTTATAGCGATTAGGCTCGCGGTTGCCCAGCTCATACa > 1:350332/1‑45 (MQ=255) tCGCAGCTTTATAGCGATTAGGCTCGCGGTTGCCCAGCTCATACAGCCAGTCGAACCa < 1:501810/58‑1 (MQ=255) tCGCAGCTTTATAGCGATTAGGCTCGCGGTTGCCCAGCTCATACAGCCAGTCGAACCa < 1:2098932/58‑1 (MQ=255) tCGCAGCTTTATAGCGATTAGGCTCGCGGTTGCCCAGCTCATACAGCCAGTCGAACCa < 1:1178281/58‑1 (MQ=255) tCGCAGCTTTATAGCGATTAGGCTCGCGGTTGCCCAGCTCATACAGCCAGTCGAACCATTTGATGATCa > 1:1838767/1‑69 (MQ=255) tCGCAGCTTTATAGCGATTAGGCTCGCGGTTGCCCAGCTCATACAGCCAGTCGAACCATTTGATGATCa > 1:3280457/1‑69 (MQ=255) tCGCAGCTTTATAGCGATTAGGCTCGCGGTTGCCCAGCTCATACAGCCAGTCGAACCATTTGATGATCa > 1:1876685/1‑69 (MQ=255) tCGCAGCTTTATAGCGATTAGGCTCGCGGTTGCCCAGCTCATACAGCCAGTCGAACCATTTGATGATCa > 1:2687182/1‑69 (MQ=255) gCAGCTTTATAGCGATTAGGCTCGCGGTTGCCCAGCTCa > 1:2983203/1‑39 (MQ=255) gCAGCTTTATAGCGATTAGGCTCGCGGTTGCCCAGCTCa > 1:2163576/1‑39 (MQ=255) gCTTTATAGCGATTAGGCTCGCGGTTGCCCAGCTCATACAGCCAGTCGAACCATTTGATGATCAGacca > 1:2889336/1‑69 (MQ=255) gCTTTATAGCGATTAGGCTCGCGGTTGCCCAGCTCATACAGCCAGTCGAACCATTTGATGATCAGacca > 1:2212473/1‑69 (MQ=255) | GCCCTTCACGCGCCAGCACGATCATATTGAAGATCGCCCCAAAGCAGAAACTGTGCAATATCGCAGCTTTATAGCGATTAGGCTCGCGGTTGCCCAGCTCATACAGCCAGTCGAACCATTTGATGATCAGACCA > minE/2418809‑2418942 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |