| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,418,890 | G→T | 47.2% | P375T (CCT→ACT) | wzyE ← | predicted Wzy protein involved in ECA polysaccharide chain elongation |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,418,890 | 0 | G | T | 47.2% | 12.4 / 56.0 | 55 | P375T (CCT→ACT) | wzyE | predicted Wzy protein involved in ECA polysaccharide chain elongation |
| Reads supporting (aligned to +/- strand): ref base G (16/13); new base T (18/8); total (34/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.05e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01 | |||||||||||
CAGCACGATCATATTGAAGATCGCCCCAAAGCAGAAACTGTGCAATATCGCAGCTTTATAGCGATTAGGCTCGCGGTTGCCCAGCTCATACAGCCAGTCGAACCATTTGATGATCAGACCAACCACGATCGCCCCG > minE/2418822‑2418957 | cAGCACGATCATATTGAAGATCGCCCCAAAGCAGAAACTGTGCAATATCGCAGCTTTATAGCGATTAgg < 1:2432164/69‑1 (MQ=255)cAGCACGATCATATTGAAGATCGCCCCAAAGCAGAAACTGTGCAATATCGCAGCTTTATAGCGATTAgg < 1:2717515/69‑1 (MQ=255)cAGCACGATCATATTGAAGATCGCCCCAAAGCAGAAACTGTGCAATATCGCAGCTTTATAGCGATTAgg < 1:3324369/69‑1 (MQ=255) aGCACGATCATATTGAAGATCGCCCCAAAGCAGAAACTGTGCAATATCGCAGCTTTATAGCGATTAgg < 1:953096/68‑1 (MQ=255) aGCACGATCATATTGAAGATCGCCCCAAAGCAGAAACTGTGCAATATCGCAGCTTTATAGCGATTAGGc > 1:1217242/1‑69 (MQ=255) aGCACGATCATATTGAAGATCGCCCCAAAGCAGAAACTGTGCAATATCGCAGCTTTATAGCGATTAGGc > 1:2861628/1‑69 (MQ=255) aGCACGATCATATTGAAGATCGCCCCAAAGCAGAAACTGTGCAATATCGCAGCTTTATAGCGATTAGGc > 1:2460556/1‑69 (MQ=255) atTGAAGATCGCCCCAAAGCAGAAACTGTGCAATATCGCAGCTTTATAGCGATTAGGCTCGCGGTTGc < 1:1186567/68‑1 (MQ=255) atTGAAGATCGCCCCAAAGCAGAAACTGTGCAATATCGCAGCTTTATAGCGATTAGGCTCGCGGTTGc < 1:1317808/68‑1 (MQ=255) atTGAAGATCGCCCCAAAGCAGAAACTGTGCAATATCGCAGCTTTATAGCGATTAGGCTCGCGGTTGc < 1:1370078/68‑1 (MQ=255) atTGAAGATCGCCCCAAAGCAGAAACTGTGCAATATCGCAGCTTTATAGCGATTAGGCTCGCGGTTGc < 1:694908/68‑1 (MQ=255) tGAAGATCGCCCCGAAGCAGAAACTGTGCAATATCGCAGCCTTATAGCGATTAGTCTcg > 1:1462487/1‑59 (MQ=255) tGAAGATCGCCCCGAAGCAGAAACTGTGCAATATCGCAGCCTTATAGCGATTAGTCTCGCGGTTGccc > 1:541787/1‑68 (MQ=255) tGAAGATCGCCCCGAAGCAGAAACTGTGCAATATCGCAGCCTTATAGCGATTAGTCTCGCGGTTGccc > 1:2905603/1‑68 (MQ=255) tGAAGATCGCCCCGAAGCAGAAACTGTGCAATATCGCAGCCTTATAGCGATTAGTCTCGCGGTTGccc > 1:1223014/1‑68 (MQ=255) tGAAGATCGCCCCGAAGCAGAAACTGTGCAATATCGCAGCCTTATAGCGATTAGTCTCGCGGTTGccc > 1:896083/1‑68 (MQ=255) tGAAGATCGCCCCGAAGCAGAAACTGTGCAATATCGCAGCCTTATAGCGATTAGTCTCGCGGTTGccc > 1:2671785/1‑68 (MQ=255) tGAAGATCGCCCCGAAGCAGAAACTGTGCAATATCGCAGCCTTATAGCGATTAGTCTCGCGGTTGccc > 1:202159/1‑68 (MQ=255) aGATCGCCCCGAAGCAGAAACTGTGCAATATCGCAGCCTTATAGCGATTAGTCTCGCGGTTGCCCAGc < 1:101314/68‑1 (MQ=255) aGATCGCCCCGAAGCAGAAACTGTGCAATATCGCAGCCTTATAGCGATTAGTCTCGCGGTTGCCCAGc < 1:2378216/68‑1 (MQ=255) aGATCGCCCCGAAGCAGAAACTGTGCAATATCGCAGCCTTATAGCGATTAGTCTCGCGGTTGCCCAGc < 1:2498539/68‑1 (MQ=255) aGATCGCCCCGAAGCAGAAACTGTGCAATATCGCAGCCTTATAGCGATTAGTCTCGCGGTTGCCCAGc < 1:2672245/68‑1 (MQ=255) aGATCGCCCCGAAGCAGAAACTGTGCAATATCGCAGCCTTATAGCGATTAGTCTCGCGGTTGCCCAGc < 1:2701189/68‑1 (MQ=255) aGATCGCCCCGAAGCAGAAACTGTGCAATATCGCAGCCTTATAGCGATTAGTCTCGCGGTTGCCCAGc < 1:3149250/68‑1 (MQ=255) aGATCGCCCCGAAGCAGAAACTGTGCAATATCGCAGCCTTATAGCGATTAGTCTCGCGGTTGCCCAGc < 1:840325/68‑1 (MQ=255) aTCGCCCCAAAGCAGAAACTGTGCAATATCGCAGCTTTATAGCGATTAGGCTCGCGGTTGCCCAGCTCa < 1:2587716/69‑1 (MQ=255) aTCGCCCCAAAGCAGAAACTGTGCAATATCGCAGCTTTATAGCGATTAGGCTCGCGGTTGCCCAGCTCa < 1:3255101/69‑1 (MQ=255) aTCGCCCCAAAGCAGAAACTGTGCAATATCGCAGCTTTATAGCGATTAGGCTCGCGGTTGCCCAGCTCa < 1:1036896/69‑1 (MQ=255) aTCGCCCCAAAGCAGAAACTGTGCAATATCGCAGCTTTATAGCGATTAGGCTCGCGGTTGCCCAGCTCa < 1:244504/69‑1 (MQ=255) cGCCCCGAAGCAGAAACTGTGCAATATCGCAGCCTTATAGCGATTAGTCTCGCGGTTGCCCAGCTCa > 1:320916/1‑67 (MQ=255) cGCCCCGAAGCAGAAACTGTGCAATATCGCAGCCTTATAGCGATTAGTCTCGCGGTTGCCCAGCTCa > 1:1103389/1‑67 (MQ=255) cGCCCCGAAGCAGAAACTGTGCAATATCGCAGCCTTATAGCGATTAGTCTCGCGGTTGCCCAGCTCa > 1:1258592/1‑67 (MQ=255) cGCCCCGAAGCAGAAACTGTGCAATATCGCAGCCTTATAGCGATTAGTCTCGCGGTTGCCCAGCTCa > 1:769780/1‑67 (MQ=255) cGCCCCGAAGCAGAAACTGTGCAATATCGCAGCCTTATAGCGATTAGTCTCGCGGTTGCCCAGCTCa > 1:1463813/1‑67 (MQ=255) cGCCCCGAAGCAGAAACTGTGCAATATCGCAGCCTTATAGCGATTAGTCTCGCGGTTGCCCAGCTCa > 1:352404/1‑67 (MQ=255) cGCCCCGAAGCAGAAACTGTGCAATATCGCAGCCTTATAGCGATTAGTCTCGCGGTTGCCCAGCTCa > 1:3243062/1‑67 (MQ=255) cGCCCCGAAGCAGAAACTGTGCAATATCGCAGCCTTATAGCGATTAGTCTCGCGGTTGCCCAGCTCa > 1:3073821/1‑67 (MQ=255) cGCCCCGAAGCAGAAACTGTGCAATATCGCAGCCTTATAGCGATTAGTCTCGCGGTTGCCCAGCTCa > 1:1898872/1‑67 (MQ=255) cGCCCCGAAGCAGAAACTGTGCAATATCGCAGCCTTATAGCGATTAGTCTCGCGGTTGCCCAGCTCa > 1:1921868/1‑67 (MQ=255) cGCCCCGAAGCAGAAACTGTGAAATATCGCAGCCTTATAGCGATTAGTCTCGCGGTTGCCCAGCTCa > 1:1473443/1‑67 (MQ=255) cAGAAACTGTGCAATATCGCAGCCTTATAGCGATTAGTCTCGCGGTTGCCCAGc < 1:2329470/54‑1 (MQ=255) tCGCAGCTTTATAGCGATTAGGCTCGCGGTTGCCCAGCTCATACa > 1:350332/1‑45 (MQ=255) tCGCAGCTTTATAGCGATTAGGCTCGCGGTTGCCCAGCTCATACAGCCAGTCGAACCa < 1:1178281/58‑1 (MQ=255) tCGCAGCTTTATAGCGATTAGGCTCGCGGTTGCCCAGCTCATACAGCCAGTCGAACCa < 1:501810/58‑1 (MQ=255) tCGCAGCTTTATAGCGATTAGGCTCGCGGTTGCCCAGCTCATACAGCCAGTCGAACCa < 1:2098932/58‑1 (MQ=255) tCGCAGCTTTATAGCGATTAGGCTCGCGGTTGCCCAGCTCATACAGCCAGTCGAACCATTTGATGATCa > 1:3430465/1‑69 (MQ=255) tCGCAGCTTTATAGCGATTAGGCTCGCGGTTGCCCAGCTCATACAGCCAGTCGAACCATTTGATGATCa > 1:2505234/1‑69 (MQ=255) tCGCAGCTTTATAGCGATTAGGCTCGCGGTTGCCCAGCTCATACAGCCAGTCGAACCATTTGATGATCa > 1:3280457/1‑69 (MQ=255) tCGCAGCTTTATAGCGATTAGGCTCGCGGTTGCCCAGCTCATACAGCCAGTCGAACCATTTGATGATCa > 1:1838767/1‑69 (MQ=255) tCGCAGCTTTATAGCGATTAGGCTCGCGGTTGCCCAGCTCATACAGCCAGTCGAACCATTTGATGATCa > 1:2687182/1‑69 (MQ=255) tCGCAGCTTTATAGCGATTAGGCTCGCGGTTGCCCAGCTCATACAGCCAGTCGAACCATTTGATGATCa > 1:1876685/1‑69 (MQ=255) gCAGCTTTATAGCGATTAGGCTCGCGGTTGCCCAGCTCa > 1:2163576/1‑39 (MQ=255) gCAGCTTTATAGCGATTAGGCTCGCGGTTGCCCAGCTCa > 1:2983203/1‑39 (MQ=255) gCTTTATAGCGATTAGGCTCGCGGTTGCCCAGCTCATACAGCCAGTCGAACCATTTGATGATCAGacca > 1:1800501/1‑69 (MQ=255) gCTTTATAGCGATTAGGCTCGCGGTTGCCCAGCTCATACAGCCAGTCGAACCATTTGATGATCAGacca > 1:2212473/1‑69 (MQ=255) gCTTTATAGCGATTAGGCTCGCGGTTGCCCAGCTCATACAGCCAGTCGAACCATTTGATGATCAGacca > 1:2889336/1‑69 (MQ=255) ttATAGCGATTAGGCTCGCGGTTGCCCAGCTCATACAGCCAGTCGAACCATTTGATGATCAGaccaac > 1:1936404/1‑68 (MQ=255) taGCGATTAGGCTCGCGGTTGCCCAGCTCAT‑‑AGCCAGTCGAACCATTTGATGATCAGACCAACCACg < 1:562782/67‑1 (MQ=255) aGCGATTAGGCTCGCGGTTGCCCAGCTCATACAGCCAGTCGAACCATTTGATGATCAGACCAACCACg < 1:2167724/68‑1 (MQ=255) ggCTCGCGGTTGCCCAGCTCATACAGCCAGTCGAACCATTTGATGATCAGACCAACCACGATCGcccc > 1:196023/1‑68 (MQ=255) ggCTCGCGGTTGCCCAGCTCATACAGCCAGTCGAACCATTTGATGATCAGACCAACCACGATCGCCCCg > 1:2734896/1‑69 (MQ=255) ggCTCGCGGTTGCCCAGCTCATACAGCCAGTCGAACCATTTGATGATCAGACCAACCACGATCGCCACg > 1:831931/1‑69 (MQ=255) | CAGCACGATCATATTGAAGATCGCCCCAAAGCAGAAACTGTGCAATATCGCAGCTTTATAGCGATTAGGCTCGCGGTTGCCCAGCTCATACAGCCAGTCGAACCATTTGATGATCAGACCAACCACGATCGCCCCG > minE/2418822‑2418957 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |