| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,420,852 | A→T | 54.5% | R79R (CGT→CGA) | rffT ← | TDP‑Fuc4NAc:lipidII transferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,420,852 | 0 | A | T | 54.5% | 4.7 / 48.5 | 44 | R79R (CGT→CGA) | rffT | TDP‑Fuc4NAc:lipidII transferase |
| Reads supporting (aligned to +/- strand): ref base A (11/9); new base T (11/13); total (22/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.63e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.63e-01 | |||||||||||
TGGGCTTAATGCCACCACTCAGCAGAGCCAGCCACAGTGTGGGATTGAACTGACCGTGGAAGAAAAAA‑CGCTGCTGACGGTTAGCTTTTGCTTTCGCGATGACCGCTTCCGCCAGCGATTTTTTCCCA > minE/2420785‑2420912 | tggGCTTAATGCCACCACTCAGCAGAGCCAGCCACAGTGTGGGATTGAACTGACCGTGGAAGAAAAAA‑c < 1:1067098/69‑1 (MQ=255)tggGCTTAATGCCACCACTCAGCAGAGCCAGCCACAGTGTGGGATTGAACTGACCGTGGAAGAAAAAA‑c < 1:2122997/69‑1 (MQ=255)tggGCTTAATGCCACCACTCAGCAGAGCCAGCCACAGTGTGGGATTGAACTGACCGTGGAAGAAAAAA‑c < 1:1136496/69‑1 (MQ=255)tggGCTTAATGCCACCACTCAGCAGAGCCAGCCACAGTGTGGGATTGAACTGACCGTGGAAGAAAAAA‑c < 1:1728389/69‑1 (MQ=255)tggGCTTAATGCCACCACTCAGCAGAGCCAGCCACAGTGTGGGATTGAACTGACCGTGGAAGAAAAAA‑c < 1:478020/69‑1 (MQ=255) gggCTTAATGCCACCACTCAGCAGAGCCAGCCACAGTGTGGGATTGAACTGACCGTGGAAGAAAAAA‑c < 1:154240/68‑1 (MQ=255) tAATGCCACCACTCAGCAGAGCCAGCCACAGTGTGGGATTGAACTGACCGTGGAAGAAAAAA‑Cgctgct < 1:1612380/69‑1 (MQ=255) caccacTCAGCAGAGCCAGCCACAGTTTGGGATTGAACTGACCGTGGAAAAAAAAT‑CGCTGCTGACg > 1:712307/1‑67 (MQ=255) caccacTCAGCAGAGCCAGCCACAGTTTGGGATTGAACTGACCGTGGAAAAAAAAT‑CGCTGCTGACg > 1:1511360/1‑67 (MQ=255) caccacTCAGCAGAGCCAGCCACAGTTTGGGATTGAACTGACCGTGGAAAAAAAAT‑CGCTGCTGACg > 1:152866/1‑67 (MQ=255) caccacTCAGCAGAGCCAGCCACAGTTTGGGATTGAACTGACCGTGGAAAAAAAAT‑CGCTGCTGACg > 1:1608112/1‑67 (MQ=255) caccacTCAGCAGAGCCAGCCACAGTTTGGGATTGAACTGACCGTGGAAAAAAAAT‑CGCTGCTGACg > 1:3228948/1‑67 (MQ=255) caccacTCAGCAGAGCCAGCCACAGTTTGGGATTGAACTGACCGTGGAAAAAAAAT‑CGCTGCTGACg > 1:166700/1‑67 (MQ=255) caccacTCAGCAGAGCCAGCCACAGTTTGGGATTGAACTGACCGTGGAAAAAAAAT‑CGCTGCTGACg > 1:2630389/1‑67 (MQ=255) caccacTCAGCAGAGCCAGCCACAGTTTGGGATTGAACTGACCGTGGAAAAAAAAT‑CGCTGCTGACg > 1:806730/1‑67 (MQ=255) caccacTCAGCAGAGCCAGCCACAGTTTGGGATTGAACTGACCGTGGAAAAAAAAT‑CGCTGCTGACg > 1:829179/1‑67 (MQ=255) caccacTCAGCAGAGCCAGCCACAGTTTGGGATTGAACTGACCGTGGAAAAAAAAT‑CGCTGCTGACg > 1:1877132/1‑67 (MQ=255) caccacTCAGCAGAGCCAGCCACAGTTTGGGATTGAACTGACCGTGGAAAAAAAAT‑CGCTGCTGACg > 1:1993513/1‑67 (MQ=255) caccacTCAGCAGAGCCAGCCACAGTTTGGGATTGAACTGACCGTGGAAAAAAAAATCGCTGCTGACg > 1:975028/1‑68 (MQ=255) agcagAGCCAGCCACAGTTTTGGATTGAACTGACCGTGGAAAAAAAAT‑CGCTGCTGACgg < 1:1029834/60‑1 (MQ=255) agcagAGCCAGCCACAGTTTGGGATTGAACTGACCGTGGAAAAAAAAT‑CGCTGCTGACgg < 1:2073240/60‑1 (MQ=255) agcagAGCCAGCCACAGTTTGGGATTGAACTGACCGTGGAAAAAAAAT‑CGCTGCTGACgg < 1:1285890/60‑1 (MQ=255) agcagAGCCAGCCACAGTTTGGGATTGAACTGACCGTGGAAAAAAAAT‑CGCTGCTGACgg < 1:420154/60‑1 (MQ=255) agcagAGCCAGCCACAGTTTGGGATTGAACTGACCGTGGAAAAAAAAT‑CGCTGCTGACgg < 1:352843/60‑1 (MQ=255) agcagAGCCAGCCACAGTTTGGGATTGAACTGACCGTGGAAAAAAAAT‑CGCTGCTGACgg < 1:3253527/60‑1 (MQ=255) agcagAGCCAGCCACAGTTTGGGATTGAACTGACCGTGGAAAAAAAAT‑CGCTGCTGACgg < 1:3064301/60‑1 (MQ=255) agcagAGCCAGCCACAGTTTGGGATTGAACTGACCGTGGAAAAAAAAT‑CGCTGCTGACgg < 1:2995508/60‑1 (MQ=255) agcagAGCCAGCCACAGTTTGGGATTGAACTGACCGTGGAAAAAAAAT‑CGCTGCTGACgg < 1:2810000/60‑1 (MQ=255) agcagAGCCAGCCACAGTTTGGGATTGAACTGACCGTGGAAAAAAAAT‑CGCTGCTGACgg < 1:2697143/60‑1 (MQ=255) agcagAGCCAGCCACAGTTTGGGATTGAACTGACCGTGGAAAAAAAAT‑CGCTGCTGACgg < 1:1683996/60‑1 (MQ=255) agcagAGCCAGCCACAGTTTGGGATTGAACTGACCGTGGAAAAAAAAT‑CGCTGCTGACgg < 1:2018374/60‑1 (MQ=255) agcagAGCCAGCCACAGTGTGGGATTGAACTGACCGTGGAAGAAAAAA‑CGCTGCTGACGGTTAGCtttt > 1:2299151/1‑69 (MQ=255) agcagAGCCAGCCACAGTGTGGGATTGAACTGACCGTGGAAGAAAAAA‑CGCTGCTGACGGTTAGCtttt > 1:493543/1‑69 (MQ=255) agcagAGCCAGCCACAGTGTGGGATTGAACTGACCGTGGAAGAAAAAA‑CGCTGCTGACGGTTAGCtttt > 1:1344158/1‑69 (MQ=255) agccaCAGTGTGGGATTGAACTGACCGTGGAAGAAAAAA‑CGCTGCTGACGGTTAGCTTTTGCTTTcgcg < 1:1395158/69‑1 (MQ=255) agccaCAGTGTGGGATTGAACTGACCGTGGAAGAAAAAA‑CGCTGCTGACGGTTAGCTTTTGCTTTcgcg < 1:833211/69‑1 (MQ=255) ccaCAGTTTGGGATTGAACTGACCGTGGAAAAAAAAT‑CGCTGCTGACgg < 1:2506587/49‑1 (MQ=38) gtgtGGGATTGAACTGACCGTGGAAGAAAAAA‑CGCTGCTGACGGTTAGCTTTTGCTTTCGCGATGACCg > 1:928875/1‑69 (MQ=255) ttGAACTGACCGTGGAAGAAAAAA‑CGCTGCTGACGGTTAGCTTTTGCTTTCGCGATGACCGCTTCCGc > 1:1651355/1‑68 (MQ=255) tGAACTGACCGTGGAAGAAAAAA‑CGCTGCTGACGGTTAGCTTTTGCTTTCGCGATGACCGCTTCCGCCa > 1:2565093/1‑69 (MQ=255) tGAACTGACCGTGGAAGAAAAAA‑CGCTGCTGACGGTTAGCTTTTGCTTTCGCGATGACCGCTTCCGCCa > 1:1738152/1‑69 (MQ=255) gACCGTGGAAGAAAAAA‑CGCTGCTGACGGTTAGCTTTTGCTTTCGCGATGACCGCTTCCGCCAGCGAtt > 1:2498870/1‑69 (MQ=255) gACCGTGGAAGAAAAAA‑CGCTGCTGACGGTTAGCTTTTGCTTTCGCGATGACCGCTTCCGCCAGCGAtt > 1:1132094/1‑69 (MQ=255) agaaAAAA‑CGCTGCTGACGGTTAGCTTTTGCTTTCGCGATGACCGCTTCCGCCAGCGATTTTTTCCCa > 1:353960/1‑68 (MQ=255) | TGGGCTTAATGCCACCACTCAGCAGAGCCAGCCACAGTGTGGGATTGAACTGACCGTGGAAGAAAAAA‑CGCTGCTGACGGTTAGCTTTTGCTTTCGCGATGACCGCTTCCGCCAGCGATTTTTTCCCA > minE/2420785‑2420912 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |