| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,423,039 | T→C | 30.2% | K143K (AAA→AAG) | rffA ← | TDP‑4‑oxo‑6‑deoxy‑D‑glucose transaminase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,423,039 | 0 | T | C | 30.2% | 100.6 / 52.4 | 109 | K143K (AAA→AAG) | rffA | TDP‑4‑oxo‑6‑deoxy‑D‑glucose transaminase |
| Reads supporting (aligned to +/- strand): ref base T (57/19); new base C (21/12); total (78/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.53e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 109 total aligned reads displayed.
CACGCCCTTTGTAAGTGGACATCACGCCCTGAGCGGCATCTTCTACCACAAACAAATTATGCTTTTTCGCCAACGCCATAATGGTGTCCATTTCGCAGGCCACACCCGCGTAATGGACCGGCACGAT > minE/2422975‑2423101 | cACGCCCTTTGTAAGTGGACATCACGCCCTGAGCGGCATCTTCTACCACAAACAAATTATGCTTTTTc > 1:1211402/1‑68 (MQ=255)cACGCCCTTTGTAAGTGGACATCACGCCCTGAGCGGCATCTTCTACCACAAACAAATTATGCTTTTTc > 1:539027/1‑68 (MQ=255)cACGCCCTTTGTAAGTGGACATCACGCCCTGAGCGGCATCTTCTACCACAAACAAATTATGCTTTTTc > 1:2111415/1‑68 (MQ=255)cACGCCCTTTGTAAGTGGACATCACGCCCTGAGCGGCATCTTCTACCACAAACAAATTATGCTTTTTc > 1:1764314/1‑68 (MQ=255) cGCCCTTTGTAAGTGGACATCACGCCCTGAGCGGCATCTTCTACCACAAACAAATTATGCTTTTTCGcc < 1:1176065/69‑1 (MQ=255) cGCCCTTTGTAAGTGGACATCACGCCCTGAGCGGCATCTTCTACCACAAACAAATTATGCTTTTTCGcc < 1:4850/69‑1 (MQ=255) tGTAAGTGGACATCACGCCCTGAGCGGCATCTTCTAGCACAAACAAATTATGCTTTTTCGCCAACGcc > 1:274272/1‑68 (MQ=255) tGTAAGTGGACATCACGCCCTGAGCGGCATCTTCTACCACAAACAAATTATGCTTTTTCGCCAACGcc > 1:66881/1‑68 (MQ=255) tGTAAGTGGACATCACGCCCTGAGCGGCATCTTCTACCACAAACAAATTATGCTTTTTCGCCAACGcc > 1:3137818/1‑68 (MQ=255) tGTAAGTGGACATCACGCCCTGAGCGGCATCTTCTACCACAAACAAATTATGCTTTTTCGCCAACGcc > 1:3187709/1‑68 (MQ=255) tGTAAGTGGACATCACGCCCTGAGCGGCATCTTCTACCACAAACAAATTATGCTTTTTCGCCAACGcc > 1:3290485/1‑68 (MQ=255) tGTAAGTGGACATCACGCCCTGAGCGGCATCTTCTACCACAAACAAATTATGCTTTTTCGCCAACGcc > 1:339776/1‑68 (MQ=255) tGTAAGTGGACATCACGCCCTGAGCGGCATCTTCTACCACAAACAAATTATGCTTTTTCGCCAACGcc > 1:1994463/1‑68 (MQ=255) tGTAAGTGGACATCACGCCCTGAGCGGCATCTTCTACCACAAACAAATTATGCTTTTTCGCCAACGcc > 1:1717055/1‑68 (MQ=255) tGTAAGTGGACATCACGCCCTGAGCGGCATCTTCTACCACAAACAAATTATGCTTTTTCGCCAACGcc > 1:905166/1‑68 (MQ=255) tGTAAGTGGACATCACGCCCTGAGCGGCATCTTCTACCACAAACAAATTATGCTTTTTCGCCAACGcc > 1:1381713/1‑68 (MQ=255) tGTAAGTGGACATCACGCCCTGAGCGGCATCTTCTACCACAAACAAATTATGCTTTTTCGCCAACGcc > 1:1272724/1‑68 (MQ=255) tGTAAGTGGACATCACGCCCTGAGCGGCATCTTCTACCACAAACAAATTATGCTTTTTCGCCAACGc > 1:1769593/1‑67 (MQ=255) aCGCCCTGAGCGGCATCTTCTACCACAAACAAATTATGCTTTTTCGCCAACGCCATAATg > 1:3332781/1‑60 (MQ=255) aCGCCCTGAGCGGCATCTTCTACCACAAACAAATTATGCTTTTTCGCCAACGCCATAATGGTGTcc > 1:505294/1‑66 (MQ=255) aCGCCCTGAGCGGCATCTTCTACCACAAACAAATTATGCTTTTTCGCCAACGCCATAATGGTGTCCAtt > 1:1686733/1‑69 (MQ=255) aCGCCCTGAGCGGCATCTTCTACCACAAACAAATTATGCTTTTTCGCCAACGCCATAATGGTGTCCAtt > 1:1166078/1‑69 (MQ=255) aCGCCCTGAGCGGCATCTTCTACCACAAACAAATTATGCTTTTTCGCCAACGCCATAATGGTGTCCAtt > 1:3314673/1‑69 (MQ=255) aCGCCCTGAGCGGCATCTTCTACCACAAACAAATTATGCTTTTTCGCCAACGCCATAATGGTGTCCAtt > 1:2813826/1‑69 (MQ=255) aCGCCCTGAGCGGCATCTTCTACCACAAACAAATTATGCTTTTTCGCCAACGCCATAATGGTGTCCAtt > 1:278187/1‑69 (MQ=255) aCGCCCTGAGCGGCATCTTCTACCACAAACAAATTATGCTTTTTCGCCAACGCCATAATGGTGTCCAtt > 1:93615/1‑69 (MQ=255) aCGCCCTGAGCGGCATCTTCTACCACAAACAAATTATGCTTTTTCGCCAACGCCATAATGGTGTCCAtt > 1:353391/1‑69 (MQ=255) aCGCCCTGAGCGGCATCTTCTACCACAAACAAATTATGCTTTTTCGCCAACGCCATAATGGTGTCCAtt > 1:1207029/1‑69 (MQ=255) aCGCCCTGAGCGGCATCTTCTACCACAAACAAATTATGCTTTTTCGCCAACGCCATAATGGTGTCCAtt > 1:503963/1‑69 (MQ=255) aCGCCCTGAGCGGCATCTTCTACCACAAACAAATTATGCTTTTTCGCCAACGCCATAATGGTGTCCAtt > 1:2293252/1‑69 (MQ=255) aCGCCCTGAGCGGCATCTTCTACCACAAACAAATTATGCTTTTTCGCCAACGCCATAATGGTGTCCAtt > 1:1508614/1‑69 (MQ=255) aCGCCCTGAGCGGCATCTTCTACCACAAACAAATTATGCTTTTTCGCCAACGCCATAATGGTGTCCAtt > 1:2170466/1‑69 (MQ=255) aCGCCCTGAGCGGCATCTTCTACCACAAACAAATTATGCTTTTTCGCCAACGCCATAATGGTGTCCAtt > 1:2157774/1‑69 (MQ=255) aCGCCCTGAGCGGCATCTTCTACCACAAACAAATTATGCTTTTTCGCCAACGCCATAATGGTGTCCAtt > 1:1337/1‑69 (MQ=255) aCGCCCTGAGCGGCATCTTCTACCACAAACAAATTATGCTTTTTCGCCAACGCCATAATGGTGTCCAtt > 1:653926/1‑69 (MQ=255) aCGCCCTGAGCGGCATCTTCTACCACAAACAAATTATGCTTTTTCGCCAACGCCATAATGGTGTCCAtt > 1:18104/1‑69 (MQ=255) aCGCCCTGAGCGGCATCTTCTACCACAAACAAATTATGCTTTTTCGCCAACGCCATAATGGTGTCCAtt > 1:2049150/1‑69 (MQ=255) aCGCCCTGAGCGGCATCTTCTACCACAAACAAATTATGCTTTTTCGCCAACGCCATAATGGTGTCCAtt > 1:755120/1‑69 (MQ=255) aCGCCCTGAGCGGCATCTTCTACCACAAACAAATTATGCTTTTTCGCCAACGCCATAATGGTGTCCAtt > 1:805178/1‑69 (MQ=255) aCGCCCTGAGCGGCATCTTCTACCACAAACAAATTATGCTTTTT‑‑CCAACGCCATAATGGTGTCCAttt > 1:2777412/1‑68 (MQ=255) tcttccACCACAAACAGATTATGCTTCTTCGCCAACGCCATAATGGTGTCCATTTCGCAGGCCACAccc < 1:377186/69‑1 (MQ=255) tcttccACCACAAACAGATTATGCTTCTTCGCCAACGCCATAATGGTGTCCATTTCGCAGGCCACAccc < 1:1618799/69‑1 (MQ=255) tcttccACCACAAACAGATTATGCTTCTTCGCCAACGCCATAATGGTGTCCATTTCGCAGGCCACAccc < 1:252742/69‑1 (MQ=255) tcttccACCACAAACAGATTATGCTTCTTCGCCAACGCCATAATGGTGTCCATTTCGCAGGCCACAccc < 1:690119/69‑1 (MQ=255) tcttccACCACAAACAGATTATGCTTCTTCGCCAACGCCATAATGGTGTCCATTTCGCAGGCCACAccc < 1:1628371/69‑1 (MQ=255) tcttccACCACAAACAGATTATGCTTCTTCGCCAACGCCATAATGGTGTCCATTTCGCAGGCCACAccc < 1:2040246/69‑1 (MQ=255) tcttccACCACAAACAGATTATGCTTCTTCGCCAACGCCATAATGGTGTCCATTTCGCAGGCCACAccc < 1:971768/69‑1 (MQ=255) tcttccACCACAAACAGATTATGCTTCTTCGCCAACGCCATAATGGTGTCCATTTCGCAGGCCACAccc < 1:2193848/69‑1 (MQ=255) tcttccACCACAAACAGATTATGCTTCTTCGCCAACGCCATAATGGTGTCCATTTCGCAGGCCACAccc < 1:3143337/69‑1 (MQ=255) tcttccACCACAAACAGATTATGCTTCTTCGCCAACGCCATAATGGTGTCCATTTCGCAGGCCACAccc < 1:1053439/69‑1 (MQ=255) ttctACCACAAACAAATTATGCTTTTTCGCCAACGCCAt > 1:953619/1‑39 (MQ=255) ttctACCACAAACAAATTATGCTTTTTCGCCAACGCCAt < 1:3208362/39‑1 (MQ=255) ttctACCACAAACAAATTATGCTTTTTCGCCAACGCCAt > 1:1355435/1‑39 (MQ=255) ttctACCACAAACAAATTATGCTTTTTCGCCAACGCCAt < 1:3321104/39‑1 (MQ=255) ttctACCACAAACAAATTATGCTTTTTCGCCAACGCCAt > 1:3363545/1‑39 (MQ=255) ttctACCACAAACAAATTATGCTTTTTCGCCAACGCCAt < 1:2875357/39‑1 (MQ=255) ttctACCACAAACAAATTATGCTTTTTCGCCAACGCCAt < 1:1644178/39‑1 (MQ=255) ttctACCACAAACAAATTATGCTTTTTCGCCAACGCCAt > 1:529160/1‑39 (MQ=255) ttctACCACAAACAAATTATGCTTTTTCGCCAACGCCAt > 1:1237383/1‑39 (MQ=255) ttctACCACAAACAAATTATGCTTTTTCGCCAACGCCAt < 1:1247600/39‑1 (MQ=255) ttctACCACAAACAAATTATGCTTTTTCGCCAACGCCATAATGGTGTCCATTTCGCAGGc > 1:3308836/1‑60 (MQ=255) ttctACCACAAACAAATTATGCTTTTTCGCCAACGCCATAATGGTGTCCATTTCGCAGGc > 1:362685/1‑60 (MQ=255) ttctACCACAAACAAATTATGCTTTTTCGCCAACGCCATAATGGTGTCCATTTCGCAGGc > 1:983425/1‑60 (MQ=255) ttctACCACAAACAAATTATGCTTTTTCGCCAACGCCATAATGGTGTCCATTTCGCAGGc > 1:3022067/1‑60 (MQ=255) ttctACCACAAACAAATTATGCTTTTTCGCCAACGCCATAATGGTGTCCATTTCGCAGGc > 1:213131/1‑60 (MQ=255) ttctACCACAAACAAATTATGCTTTTTCGCCAACGCCATAATGGTGTCCATTTCGCAGGc > 1:2908217/1‑60 (MQ=255) ttctACCACAAACAAATTATGCTTTTTCGCCAACGCCATAATGGTGTCCATTTCGCAGGc > 1:2895987/1‑60 (MQ=255) ttctACCACAAACAAATTATGCTTTTTCGCCAACGCCATAATGGTGTCCATTTCGCAGGc > 1:2209969/1‑60 (MQ=255) ttctACCACAAACAAATTATGCTTTTTCGCCAACGCCATAATGGTGTCCATTTCGCAGGCCACACCcg > 1:3201/1‑68 (MQ=255) ttctACCACAAACAAATTATGCTTTTTCGCCAACGCCATAATGGTGTCCATTTCGCAGGCCACACCcg > 1:459149/1‑68 (MQ=255) tctACCACAAACAAATTATGCTTTTTCGCCAACGCCAt < 1:876370/38‑1 (MQ=255) aCCACAAACAAATTATGCTTTTTCGCCAACGCCATAATGGTGTCCATTTCGCAGGCCACACCCGCGt < 1:22639/67‑1 (MQ=255) aCCACAAACAAATTATGCTTTTTCGCCAACGCCATAATGGTGTCCATTTCGCAGGCCACACCCGCGt < 1:1121945/67‑1 (MQ=255) aCCACAAACAAATTATGCTTTTTCGCCAACGCCATAATGGTGTCCATTTCGCAGGCCACACCCGCGt < 1:1125989/67‑1 (MQ=255) aCCACAAACAAATTATGCTTTTTCGCCAACGCCATAATGGTGTCCATTTCGCAGGCCACACCCGCGt < 1:1609921/67‑1 (MQ=255) aCCACAAACAAATTATGCTTTTTCGCCAACGCCATAATGGTGTCCATTTCGCAGGCCACACCCGCGt < 1:1660643/67‑1 (MQ=255) aCCACAAACAAATTATGCTTTTTCGCCAACGCCATAATGGTGTCCATTTCGCAGGCCACACCCGCGt < 1:619325/67‑1 (MQ=255) aCCACAAACAAATTATGCTTTTTCGCCAACGCCATAATGGTGTCCATTTCGCAGGCCACACCCGCGt < 1:3132725/67‑1 (MQ=255) aCCACAAACAAATTATGCTTTTTCGCCAACGCCATAATGGTGTCCATTTCGCAGGCCACACCCGCGt < 1:2379723/67‑1 (MQ=255) acaaacagATTATGCTTCTTCGCCAACGCCATAATGGTGTCCAttta > 1:3047233/1‑46 (MQ=38) acaaacagATTATGCTTCTTCGCCAACGCCATAATGGTGTCCATTTc > 1:1836275/1‑47 (MQ=38) acaaacagATTATGCTTCTTCGCCAACGCCATAATGGTGTCCATTTc > 1:310048/1‑47 (MQ=38) acaaacagATTATGCTTCTTCGCCAACGCCATAATGGTGTCCATTTc > 1:3051308/1‑47 (MQ=38) acaaacagATTATGCTTCTTCGCCAACGCCATAATGGTGTCCATTTc > 1:1176338/1‑47 (MQ=38) acaaacagATTATGCTTCTTCGCCAACGCCATAATGGTGTCCATTTc > 1:1204012/1‑47 (MQ=38) acaaacagATTATGCTTCTTCGCCAACGCCATAATGGTGTCCATTTc > 1:3233388/1‑47 (MQ=38) acaaacagATTATGCTTCTTCGCCAACGCCATAATGGTGTCCATTTc > 1:302216/1‑47 (MQ=38) acaaacagATTATGCTTCTTCGCCAACGCCATAATGGTGTCCATTTc > 1:791382/1‑47 (MQ=38) acaaacagATTATGCTTCTTCGCCAACGCCATAATGGTGTCCATTTc > 1:786595/1‑47 (MQ=38) acaaacagATTATGCTTCTTCGCCAACGCCATAATGGTGTCCATTTc > 1:24693/1‑47 (MQ=38) acaaacagATTATGCTTCTTCGCCAACGCCATAATGGTGTCCATTTc > 1:2098145/1‑47 (MQ=38) acaaacagATTATGCTTCTTCGCCAACGCCATAATGGTGTCCATTTc > 1:2936762/1‑47 (MQ=38) acaaacagATTATGCTTCTTCGCCAACGCCATAATGGTGTCCATTTc > 1:2591945/1‑47 (MQ=38) acaaacagATTATGCTTCTTCGCCAACGCCATAATGGTGTCCATTTc > 1:592294/1‑47 (MQ=38) acaaacagATTATGCTTCTTCGCCAACGCCATAATGGTGTCCATTTc > 1:2198694/1‑47 (MQ=38) acaaacagATTATGCTTCTTCGCCAACGCCATAATGGTGTCCATTTc > 1:2554846/1‑47 (MQ=38) acaaacagATTATGCTTCTTCGCCAACGCCATAATGGTGTCCATTTc > 1:2548327/1‑47 (MQ=38) acaaacagATTATGCTTCTTCGCCAACGCCATAATGGTGTCCATTTc > 1:2502222/1‑47 (MQ=38) aTGCTTTTTCGCCAACGCCATAATGGTGTCCATTTCGCAGGCCACACCCGCGTAATGGACCGTCACGAt < 1:852/69‑1 (MQ=255) aTGCTTTTTCGCCAACGCCATAATGGTGTCCATTTCGCAGGCCACACCCGCGTAATGGACCGGCACGAt < 1:1914637/69‑1 (MQ=255) | CACGCCCTTTGTAAGTGGACATCACGCCCTGAGCGGCATCTTCTACCACAAACAAATTATGCTTTTTCGCCAACGCCATAATGGTGTCCATTTCGCAGGCCACACCCGCGTAATGGACCGGCACGAT > minE/2422975‑2423101 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |