| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,423,180 | A→G | 31.2% | V96V (GTT→GTC) | rffA ← | TDP‑4‑oxo‑6‑deoxy‑D‑glucose transaminase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,423,180 | 0 | A | G | 31.2% | 100.1 / 58.0 | 115 | V96V (GTT→GTC) | rffA | TDP‑4‑oxo‑6‑deoxy‑D‑glucose transaminase |
| Reads supporting (aligned to +/- strand): ref base A (19/60); new base G (14/22); total (33/82) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.22e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 138 total aligned reads displayed.
TCGGTGATCGCCGCTTCAATCAGCGTTTCGTCGATGTTCATGGTGTCCGGGCGAACATCCACAAAAACGATTTTTGCGCCACGCAGCACAAAGGCATTGGCGGTGGAGACAAAGGTGTAGCTCGGCATGATC > minE/2423115‑2423246 | tCGGTGATCGCCGCTTCTATCAGCGTTTCGTCGATGTTCATGGTGTCCGGGCGAACATCCACAAAGAc < 1:2037309/68‑1 (MQ=255)tCGGTGATCGCCGCTTCAATCAGCGTTTCGTCGATGTTCATGGTGTCCGGGCGAACATCCACAAAGAc < 1:485679/68‑1 (MQ=255)tCGGTGATCGCCGCTTCAATCAGCGTTTCGTCGATGTTCATGGTGTCCGGGCGAACATCCACAAAGAc < 1:3274193/68‑1 (MQ=255)tCGGTGATCGCCGCTTCAATCAGCGTTTCGTCGATGTTCATGGTGTCCGGGCGAACATCCACAAAGAc < 1:2191329/68‑1 (MQ=255)tCGGTGATCGCCGCTTCAATCAGCGTTTCGTCGATGTTCATGGTGTCCGGGCGAACATCCACAAAGAc < 1:3154617/68‑1 (MQ=255)tCGGTGATCGCCGCTTCAATCAGCGTTTCGTCGATGTTCATGGTGTCCGGGCGAACATCCACAAAGAc < 1:2434957/68‑1 (MQ=255)tCGGTGATCGCCGCTTCAATCAGCGTTTCGTCGATGTTCATGGTGTCCGGGCGAACATCCACAAAGAc < 1:1289215/68‑1 (MQ=255)tCGGTGATCGCCGCTTCAATCAGCGTTTCGTCGATGTTCATGGTGTCCGGGCGAACATCCACAAAGAc < 1:2764571/68‑1 (MQ=255)tCGGTGATCGCCGCTTCAATCAGCGTTTCGTCGATGTTCATGGTGTCCGGGCGAACATCCACAAAGAc < 1:53998/68‑1 (MQ=255)tCGGTGATCGCCGCTTCAATCAGCGTTTCGTCGATGTTCATGGTGTCCGGGCGAACATCCACAAAGAc < 1:2905107/68‑1 (MQ=255)tCGGTGATCGCCGCTTCAATCAGCGTTTCGTCGATGTTCATGGTGTCCGGGCGAACATCCACAAAGAc < 1:1866077/68‑1 (MQ=255)tCGGTGATCGCCGCTTCAATCAGCGTTTCGTCGATGTTCATGGTGTCCGGGCGAACATCCACAAAGAc < 1:190561/68‑1 (MQ=255)tCGGTGATCGCCGCTTCAATCAGCGTTTCGTCGATGTTCATGGTGTCCGGGCGAACATCCACAAAGAc < 1:110037/68‑1 (MQ=255)tCGGGGATCGCCGCTTCAATCAGCGTTTCGTCGATGTTCATGGTGTCCGGGCGAACATCCACAAAGAc < 1:1920828/68‑1 (MQ=255) cGGTGATCGCCGCTTCAATCAGCGTTTCGTCGATGTTCATGGTGTACGGGCGAACATCCACAAAGAc < 1:1056429/67‑1 (MQ=255) aTCGCCGCTTCAATCAGCGTTTCGTCGATGTTCATGGTGTCCGGGCGAACATCCACAAAGACGAttttc > 1:3155446/1‑68 (MQ=255) aTCGCCGCTTCAATCAGCGTTTCGTCGATGTTCATGGTGTCCGGGCGAACATCCACAAAGACGAttttc > 1:2943531/1‑68 (MQ=255) aTCGCCGCTTCAATCAGCGTTTCGTCGATGTTCATGGTGTCCGGGCGAACATCCACAAAGACGAttttc > 1:1669744/1‑68 (MQ=255) aTCGCCGCTTCAATCAGCGTTTCGTCGATGTTCATGGTGTCCGGGCGAACATCCACAAAGACGAttttc > 1:2344830/1‑68 (MQ=255) aTCGCCGCTTCAATCAGCGTTTCGTCGATGTTCATGGTGTCCGGGCGAACATCCACAAAGACGAttttc > 1:111385/1‑68 (MQ=255) aTCGCCGCTTCAATCAGCGTTTCGTCGATGTTCATGGTGTCCGGGCGAACATCCACAAAGACGAttttc > 1:236292/1‑68 (MQ=255) aTCGCCGCTTCAATCAGCGTTTCGTCGATGTTCATGGTGTCCGGGCGAACATCCACAAAGACGAttttc > 1:745179/1‑68 (MQ=255) aTCGCCGCTTCAATCAGCGTTTCGTCGATGTTCATGGTGTCCGGGCGAACATCCACAAAGACGAttttc > 1:475280/1‑68 (MQ=255) aTCGCCGCTTCAATCAGCGTTTCGTCGATGTTCATGGTGTCCGGGCGAACATCCACAAAGACGAtttt > 1:2216038/1‑68 (MQ=255) cgccgcTTCAATCAGCGTTTCGTCGATGTTCATGGTGTCCGGGCGAACATCCACGAAAACGATTTTTgc > 1:3004382/1‑69 (MQ=255) cgccgcTTCAATCAGCGTTTCGTCGATGTTCATGGTGTCCGGGCGAACATCCACAAAAACGATTTTTgc > 1:1390094/1‑69 (MQ=255) gcTTCAATCAGCGTTTCGTCGATGTTCATGGTGTCCGGGCGAACATCCACAAAAACGATTTTTGCGCCa < 1:1558812/69‑1 (MQ=255) aTCAGCGTTTCGTCGATGTTCATGGTGTCCGGGCGAACATCCACAAAAACGATTTTTGCGCCACgca < 1:1645098/67‑1 (MQ=255) aTCAGCGTTTCGTCGATGTTCATGGTGTCCGGGCGAACATCCACAAAAACGATTTTTGCGCCACgca < 1:1230736/67‑1 (MQ=255) tttCGTCTATGTTCATGGTGTCCGGGCGAACATCCACAAAAACGATTTTTGCGCCACGCAGCACAAAgg < 1:1282096/69‑1 (MQ=255) tttCGTCGATGTTCATGGTGTCCGGGCGAACATCCACAAAAACGATTTTTGCGCCACGCAGCACAAAgg < 1:2523802/69‑1 (MQ=255) tttCGTCGATGTTCATGGTGTCCGGGCGAACATCCACAAAAACGATTTTTGCGCCACGCAGCACAAAgg < 1:264349/69‑1 (MQ=255) tttCGTCGATGTTCATGGTGTCCGGGCGAACATCCACAAAAACGATTTTTGCGCCACGCAGCACAAAgg < 1:2794493/69‑1 (MQ=255) tttCGTCGATGTTCATGGTGTCCGGGCGAACATCCACAAAAACGATTTTTGCGCCACGCAGCACAAAgg < 1:2264360/69‑1 (MQ=255) tttCGTCGATGTTCATGGTGTCCGGGCGAACATCCACAAAAACGATTTTTGCGCCACGCAGCACAAAgg < 1:2458031/69‑1 (MQ=255) tttCGTCGATGTTCATGGTGTCCGGGCGAACATCCACAAAAACGATTTTTGCGCCACGCAGCACAAAgg < 1:3080815/69‑1 (MQ=255) tttCGTCGATGTTCATGGTGTCCGGGCGAACATCCACAAAAACGATTTTTGCGCCACGCAGCACAAAgg < 1:2820695/69‑1 (MQ=255) tttCGTCGATGTTCATGGTGTCCGGGCGAACATCCACAAAAACGATTTTTGCGCCACGCAGCACAAAgg < 1:1870891/69‑1 (MQ=255) tttCGTCGATGTTCATGGTGTCCGGGCGAACATCCACAAAAACGATTTTTGCGCCACGCAGCACAAAgg < 1:1789903/69‑1 (MQ=255) tttCGTCGATGTTCATGGTGTCCGGGCGAACATCCACAAAAACGATTTTTGCGCCACGCAGCACAAAgg < 1:1749461/69‑1 (MQ=255) tttCGTCGATGTTCATGGTGTCCGGGCGAACATCCACAAAAACGATTTTTGCGCCACGCAGCACAAAgg < 1:1576930/69‑1 (MQ=255) tttCGTCGATGTTCATGGTGTCCGGGCGAACATCCACAAAAACGATTTTTGCGCCACGCAGCACAAAgg < 1:1532289/69‑1 (MQ=255) tttCGTCGATGTTCATGGTGTCCGGGCGAACATCCACAAAAACGATTTTTGCGCCACGCAGCACAAAgg < 1:3340795/69‑1 (MQ=255) tttCGTCGATGTTCATGGTGTCCGGGCGAACATCCACAAAAACGATTTTTGCGCCACGCAGCACAAAgg < 1:1085662/69‑1 (MQ=255) ttCGTCGATGTTCATGGTGTCCGGGCGAACATCCACAAAAACGATTTTTGCGCCACGCAGCACAAAgg < 1:2920749/68‑1 (MQ=255) ttCGTCGATGTTCATGGTGTCCGGGCGAACATCCACAAAAACGATTTTTGCGCCACGCAGCACAAAgg < 1:891804/68‑1 (MQ=255) ttCGTCGATGTTCATGGTGTCCGGGCGAACATCCACAAAAACGATTTTTGCGCCACGCAGCACAAATGc < 1:1305593/69‑1 (MQ=255) ttCGTCGATGTTCATGGTGTCCGGGCGAACATCCACAAAAACGATTTTTGCGCCACGCAGCACAAAGGc < 1:2362752/69‑1 (MQ=255) ttCGTCGATGTTCATGGTGTCCGGGCGAACATCCACAAAAACGATTTTTGCGCCACGCAGCACAAAGGc < 1:545416/69‑1 (MQ=255) ttCGTCGATGTTCATGGTGTCCGGGCGAACATCCACAAAAACGATTTTTGCGCCACGCAGCACAAAGGc < 1:968028/69‑1 (MQ=255) ttCGTCGATGTTCATGGTGTCCGGGCGAACATCCACAAAAACGATTTTTGCGCCACGCAGCACAAAGGc < 1:1639231/69‑1 (MQ=255) tcgtcgATGTTCATGGTGTCCGGGCGAACATCCACAAAAACGATTTTTGCGCCACGCAGCACAAAGGc < 1:1179264/68‑1 (MQ=255) gATGTTCATGGTGTCCGGGCGAACATCCACAAAGAc < 1:1680524/36‑1 (MQ=255) aTGTTCATGGTGTCCGGGCGAACATCCACAAAGAc < 1:2144308/35‑1 (MQ=255) cATGGTGTCCGGGCGAACATCCACAAAAACGATTTTTGCGCCACGCAGCACAAAgg < 1:1487431/56‑1 (MQ=255) cATGGTGTCCGGGCGAACATCCACAAAAACGATTTTTGCGCCACGCAGCACAAAgg < 1:2373493/56‑1 (MQ=255) cATGGTGTCCGGGCGAACATCCACAAAAACGATTTTTGCGCCACGCAGCACAAAgg < 1:2261204/56‑1 (MQ=255) cATGGTGTCCGGGCGAACATCCACAAAAACGATTTTTGCGCCACGCAGCACAAAgg < 1:362586/56‑1 (MQ=255) cATGGTGTCCGGGCGAACATCCACAAAAACGATTTTTGCGCCACGCAGCACAAAgg < 1:1112546/56‑1 (MQ=255) cATGGTGTCCGGGCGAACATCCACAAAAACGATTTTTGCGCCACGCAGCACAAAgg < 1:2128332/56‑1 (MQ=255) cATGGTGTCCGGGCGAACATCCACAAAAACGATTTTTGCGCCACGCAGCACAAAgg < 1:1490891/56‑1 (MQ=255) cATGGTGTCCGGGCGAACATCCACAAAAACGATTTTTGCGCCACGCAGCACAAAgg < 1:1170720/56‑1 (MQ=255) cATGGTGTCCGGGCGAACATCCACAAAAACGATTTTTGCGCCACGCAGCACAAAGGCATTGGCGGTGga > 1:544029/1‑69 (MQ=255) cATGGTGTCCGGGCGAACATCCACAAAAACGATTTTTGCGCCACGCAGCACAAAGGCATTGGCGGTGga > 1:3221971/1‑69 (MQ=255) cATGGTGTCCGGGCGAACATCCACAAAAACGATTTTTGCGCCACGCAGCACAAAGGCATTGGCGGTGga > 1:1694425/1‑69 (MQ=255) cATGGTGTCCGGGCGAACATCCACAAAAACGATTTTTGCGCCACGCAGCACAAAGGCATTGGCGGTGga > 1:717246/1‑69 (MQ=255) cATGGTGTCCGGGCGAACATCCACAAAAACGATTTTTGCGCCACGCAGCACAAAGGCATTGGCGGTGga > 1:2720320/1‑69 (MQ=255) cATGGTGTCCGGGCGAACATCCACAAAAACGATTTTTGCGCCACGCAGCACAAAGGCATTGGCGGTGga > 1:2456518/1‑69 (MQ=255) gtgtCCGGGCGAACATCCACAAAAACGATTTTTGCGCCACGCAGCACAAAGGCATTGGCGGTGgaga > 1:3052311/1‑67 (MQ=255) cGGGCGAACATCCACAAAAACGATTTTTGCGCCACGCAGCACAAAGGCATTGGCGGTGGAGACa < 1:2943744/64‑1 (MQ=255) cGGGCGAACATCCACAAAAACGATTTTTGCGCCACGCAGCACAAAGGCATTGGCGGTGGAGACa < 1:2063147/64‑1 (MQ=255) ggCGAACATCCACAAAAACGATTTTTGCGCCACGCAGCACAAAGGCATTGGCGGTGGAGACAAAGgtg < 1:450771/68‑1 (MQ=255) ggCGAACATCCACAAAAACGATTTTTGCGCCACGCAGCACAAAGGCATTGGCGGTGGAGACAAAGgtg < 1:2828139/68‑1 (MQ=255) ggCGAACATCCACAAAAACGATTTTTGCGCCACGCAGCACAAAGGCATTGGCGGTGGAGACAAAGgtg < 1:893653/68‑1 (MQ=255) gCGAACATCCACAAAAACGATTTTTGCGCCACGCAGCACAAAGGCATTGGCGGTGGAGACAAAGgtg < 1:434659/67‑1 (MQ=255) cATCCACAAAAACGATTTTTGCGCCACGCAGCACAAAGGCATTGGCGGTGGAGACa < 1:2516211/56‑1 (MQ=255) cacaAAAACGATTTTTGCGCCACGCAGCACAAAGGCATTg > 1:987502/1‑40 (MQ=255) cacaAAAACGATTTTTGCGCCACGCAGCACAAAGGCATTGGCGGTGGAGACAAAGGTGTAGCTCGGCAt > 1:1449194/1‑69 (MQ=255) cacaAAAACGATTTTTGCGCCACGCAGCACAAAGGCATTGGCGGTGGAGACAAAGGTGTAGCTCGGCAt > 1:74441/1‑69 (MQ=255) cacaAAAACGATTTTTGCGCCACGCAGCACAAAGGCATTGGCGGTGGAGACAAAGGTGTAGCTCGGCAt > 1:1129222/1‑69 (MQ=255) cacaAAAACGATTTTTGCGCCACGCAGCACAAAGGCATTGGCGGTGGAGACAAAGGTGTAGCTCGGCAt > 1:1798801/1‑69 (MQ=255) cacaAAAACGATTTTTGCGCCACGCAGCACAAAGGCATTGGCGGTGGAGACAAAGGTGTAGCTCGGCAt > 1:1209665/1‑69 (MQ=255) caAAAACGATTTTTGCGCCACGCAGCACAAAGGCATTGGCGGTGGAGACAAAGGTGTAGCTCGGCATg < 1:3427535/68‑1 (MQ=255) caAAAACGATTTTTGCGCCACGCAGCACAAAGGCATTGGCGGTGGAGACAAAGGTGTAGCTCGGCATg < 1:232948/68‑1 (MQ=255) caAAAACGATTTTTGCGCCACGCAGCACAAAGGCATTGGCGGTGGAGACAAAGGTGTAGCTCGGCATg < 1:447913/68‑1 (MQ=255) caAAAACGATTTTTGCGCCACGCAGCACAAAGGCATTGGCGGTGGAGACAAAGGTGTAGCTCGGCATg < 1:2891119/68‑1 (MQ=255) ataaaCGATTTTTGCGCCACGAAGCACAAAGGCATTGGCGGTGGAGACAAAGGTGTAGCTCGGCATg < 1:2083252/65‑1 (MQ=255) aaaaaCGATTTTTGCGCCACGCAGCACAAAGGCATTGGCGGTGGAGACAAAGGTGTAGCTCGGCATGAt < 1:3263417/69‑1 (MQ=255) aaaaaCGATTTTTGCGCCACGCAGCACAAAGGCATTGGCGGTGGAGACAAAGGTGTAGCTCGGCATGAt < 1:1370307/69‑1 (MQ=255) aaaaaCGATTTTTGCGCCACGCAGCACAAAGGCATTGGCGGTGGAGACAAAGGTGTAGCTCGGCATGAt < 1:1985187/69‑1 (MQ=255) aaaaaCGATTTTTGCGCCACGCAGCACAAAGGCATTGGCGGTGGAGACAAAGGTGTAGCTCGGCATGAt < 1:2292792/69‑1 (MQ=255) aaaaCGATTTTTGCGCCACGCAGCACAAAGGCATTGGCGGTGGAGACAAAGGTGTAGCTCGGCATGATc < 1:1230708/69‑1 (MQ=255) aaaaCGATTTTTGCGCCACGCAGCACAAAGGCATTGGCGGTGGAGACAAAGGTGTAGCTCGGCATGATc < 1:662782/69‑1 (MQ=255) aaaaCGATTTTTGCGCCACGCAGCACAAAGGCATTGGCGGTGGAGACAAAGGTGTAGCTCGGCATGATc < 1:666800/69‑1 (MQ=255) aaaaCGATTTTTGCGCCACGCAGCACAAAGGCATTGGCGGTGGAGACAAAGGTGTAGCTCGGCATGATc < 1:394027/69‑1 (MQ=255) aaaaCGATTTTTGCGCCACGCAGCACAAAGGCATTGGCGGTGGAGACAAAGGTGTAGCTCGGCATGATc < 1:354024/69‑1 (MQ=255) aaaaCGATTTTTGCGCCACGCAGCACAAAGGCATTGGCGGTGGAGACAAAGGTGTAGCTCGGCATGATc < 1:1751545/69‑1 (MQ=255) aaaaCGATTTTTGCGCCACGCAGCACAAAGGCATTGGCGGTGGAGACAAAGGTGTAGCTCGGCATGATc < 1:806606/69‑1 (MQ=255) aaaaCGATTTTTGCGCCACGCAGCACAAAGGCATTGGCGGTGGAGACAAAGGTGTAGCTCGGCATGATc < 1:1772359/69‑1 (MQ=255) aaaaCGATTTTTGCGCCACGCAGCACAAAGGCATTGGCGGTGGAGACAAAGGTGTAGCTCGGCATGATc < 1:2253164/69‑1 (MQ=255) | TCGGTGATCGCCGCTTCAATCAGCGTTTCGTCGATGTTCATGGTGTCCGGGCGAACATCCACAAAAACGATTTTTGCGCCACGCAGCACAAAGGCATTGGCGGTGGAGACAAAGGTGTAGCTCGGCATGATC > minE/2423115‑2423246 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |