| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,425,198 | A→G | 47.3% | D298D (GAT→GAC) | rffG ← | dTDP‑glucose 4,6‑dehydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,425,198 | 0 | A | G | 47.3% | 5.8 / 98.3 | 110 | D298D (GAT→GAC) | rffG | dTDP‑glucose 4,6‑dehydratase |
| Reads supporting (aligned to +/- strand): ref base A (36/22); new base G (37/15); total (73/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.19e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.07e-01 | |||||||||||
Only 100 of 111 total aligned reads displayed.
AAAGGTTTCCTGCGGCAGCCAGCCAAGTTCACGGGCAATTTTCGAAGCATCAATGGCATAACGCAGATCATGCCCCGGACGGTCAGCGACAAAGGTGATCAGGTCACGATAGTGCGCCACGCCGTGCGG > minE/2425132‑2425260 | aaaGGTTTCCTGCGGCAGCCAGCCAAGTTCACGGGCAATTTTCGAAGCATCAATGGCATAACGCAGAt < 1:1933172/68‑1 (MQ=255)aaaGGTTTCCTGCGGCAGCCAGCCAAGTTCACGGGCAATTTTCGAAGCATCAATGGCATAACGCAGAt < 1:2431154/68‑1 (MQ=255)aaaGGTTTCCTGCGGCAGCCAGCCAAGTTCACGGGCAATTTTCGAAGCATCAATGGCATAACGCAGAt < 1:1393761/68‑1 (MQ=255)aaaGGTTTCCTGCGGCAGCCAGCCAAGTTCACGGGCAATTTTCGAAGCATCAATGGCATAACGCAGAt < 1:2238063/68‑1 (MQ=255) tCACGGGCAATTTTCGAAGCATCAATGGCATAGCGTAGGTCATGc > 1:3174250/1‑45 (MQ=25) tCACGGGCAATTTTCGAAGCATCAATGGCATAGCGTAGGTCATGCCCTGGACGGTCAGCGACAAAGGt > 1:2149608/1‑68 (MQ=255) tCACGGGCAATTTTCGAAGCATCAATGGCATAGCGTAGGTCATGCCCTGGACGGTCAGCGACAAAGGTg > 1:3259737/1‑69 (MQ=255) tCACGGGCAATTTTCGAAGCATCAATGGCATAGCGTAGGTCATGCCCTGGACGGTCAGCGACAAAGGTg > 1:963546/1‑69 (MQ=255) tCACGGGCAATTTTCGAAGCATCAATGGCATAGCGTAGGTCATGCCCTGGACGGTCAGCGACAAAGGTg > 1:3238583/1‑69 (MQ=255) tCACGGGCAATTTTCGAAGCATCAATGGCATAGCGTAGGTCATGCCCTGGACGGTCAGCGACAAAGGTg > 1:3191956/1‑69 (MQ=255) tCACGGGCAATTTTCGAAGCATCAATGGCATAGCGTAGGTCATGCCCTGGACGGTCAGCGACAAAGGTg > 1:2103076/1‑69 (MQ=255) tCACGGGCAATTTTCGAAGCATCAATGGCATAGCGTAGGTCATGCCCTGGACGGTCAGCGACAAAGGTg > 1:2106400/1‑69 (MQ=255) tCACGGGCAATTTTCGAAGCATCAATGGCATAGCGTAGGTCATGCCCTGGACGGTCAGCGACAAAGGTg > 1:2109846/1‑69 (MQ=255) tCACGGGCAATTTTCGAAGCATCAATGGCATAGCGTAGGTCATGCCCTGGACGGTCAGCGACAAAGGTg > 1:3096813/1‑69 (MQ=255) tCACGGGCAATTTTCGAAGCATCAATGGCATAGCGTAGGTCATGCCCTGGACGGTCAGCGACAAAGGTg > 1:301394/1‑69 (MQ=255) tCACGGGCAATTTTCGAAGCATCAATGGCATAGCGTAGGTCATGCCCTGGACGGTCAGCGACAAAGGTg > 1:2998530/1‑69 (MQ=255) tCACGGGCAATTTTCGAAGCATCAATGGCATAGCGTAGGTCATGCCCTGGACGGTCAGCGACAAAGGTg > 1:2548801/1‑69 (MQ=255) tCACGGGCAATTTTCGAAGCATCAATGGCATAGCGTAGGTCATGCCCTGGACGGTCAGCGACAAAGGTg > 1:2742933/1‑69 (MQ=255) tCACGGGCAATTTTCGAAGCATCAATGGCATAGCGTAGGTCATGCCCTGGACGGTCAGCGACAAAGGTg > 1:2698946/1‑69 (MQ=255) tCACGGGCAATTTTCGAAGCATCAATGGCATAGCGTAGGTCATGCCCTGGACGGTCAGCGACAAAGGTg > 1:2723860/1‑69 (MQ=255) tCACGGGCAATTTTCGAAGCATCAATGGCATAGCGTAGGTCATGCCCTGGACGGTCAGCGACAAAGGTg > 1:1822067/1‑69 (MQ=255) tCACGGGCAATTTTCGAAGCATCAATGGCATAGCGTAGGTCATGCCCTGGACGGTCAGCGACAAAGGTg > 1:1153642/1‑69 (MQ=255) tCACGGGCAATTTTCGAAGCATCAATGGCATAGCGTAGGTCATGCCCTGGACGGTCAGCGACAAAGGTg > 1:1202312/1‑69 (MQ=255) tCACGGGCAATTTTCGAAGCATCAATGGCATAGCGTAGGTCATGCCCTGGACGGTCAGCGACAAAGGTg > 1:754019/1‑69 (MQ=255) tCACGGGCAATTTTCGAAGCATCAATGGCATAGCGTAGGTCATGCCCTGGACGGTCAGCGACAAAGGTg > 1:708868/1‑69 (MQ=255) tCACGGGCAATTTTCGAAGCATCAATGGCATAGCGTAGGTCATGCCCTGGACGGTCAGCGACAAAGGTg > 1:3371803/1‑69 (MQ=255) tCACGGGCAATTTTCGAAGCATCAATGGCATAGCGTAGGTCATGCCCTGGACGGTCAGCGACAAAGGTg > 1:474757/1‑69 (MQ=255) tCACGGGCAATTTTCGAAGCATCAATGGCATAACGCAGATCATGCCCCGGACGGTCAGCGACAAAGGTg < 1:2729314/69‑1 (MQ=255) tCACGGGCAATTTTCGAAGCATCAATGGCATAACGCAGATCATGCCCCGGACGGTCAGCGACAAAGGTg < 1:2278307/69‑1 (MQ=255) tCACGGGCAATTTTCGAAGCATCAATGGCATAACGCAGATCATGCCCCGGACGGTCAGCGACAAAGGTg < 1:3102146/69‑1 (MQ=255) tCACGGG‑AATTTTCGAAGCATCAATGGCATAGCGTAGGTCATGCCCTGGACGGTCAGCGACAAAGGTg > 1:225904/1‑68 (MQ=255) gggCAATTTTCGAAGCATCAATGGCATAACGCAGa < 1:76191/35‑1 (MQ=255) cAATTTTCGAAGCATCAATGGCATAACGCAGATCATGCCCCGGACGGTCAGCGACAAAGGTGATCAGGt > 1:2904257/1‑69 (MQ=255) cAATTTTCGAAGCATCAATGGCATAACGCAGATCATGCCCCGGACGGTCAGCGACAAAGGTGATCAGGt > 1:1116580/1‑69 (MQ=255) aaTTTTCGAAGCATCAATGGCATAACGCAGATCATGCCCCGGACGGTCAGCGACAAAgg > 1:1870463/1‑59 (MQ=255) aaTTTTCGAAGCATCAATGGCATAACGCAGATCATGCCCCGGACGGTCAGCGACAAAGGt < 1:3007018/60‑1 (MQ=255) aaTTTTCGAAGCATCAATGGCATAACGCAGATCATGCCCCGGACGGTCAGCGACAAAGGt > 1:3312132/1‑60 (MQ=255) aaTTTTCGAAGCATCAATGGCATAACGCAGATCATGCCCCGGACGGTCAGCGACAAAGGt > 1:2717823/1‑60 (MQ=255) aaTTTTCGAAGCATCAATGGCATAACGCAGATCATGCCCCGGACGGTCAGCGACAAAGGt < 1:825403/60‑1 (MQ=255) aaTTTTCGAAGCATCAATGGCATAACGCAGATCATGCCCCGGACGGTCAGCGACAAAGGt < 1:1109555/60‑1 (MQ=255) aaTTTTCGAAGCATCAATGGCATAACGCAGATCATGCCCCGGACGGTCAGCGACAAAGGt > 1:2771915/1‑60 (MQ=255) aaTTTTCGAAGCATCAATGGCATAACGCAGATCATGCCCCGGACGGTCAGCGACAAAGGt > 1:1701144/1‑60 (MQ=255) aaTTTTCGAAGCATCAATGGCATAACGCAGATCATGCCCCGGACGGTCAGCGACAAAGGt > 1:2345012/1‑60 (MQ=255) aaTTTTCGAAGCATCAATGGCATAACGCAGATCATGCCCCGGACGGTCAGCGACAAAGGt > 1:3136484/1‑60 (MQ=255) aaTTTTCGAAGCATCAATGGCATAACGCAGATCATGCCCCGGACGGTCAGCGACAAAGGt > 1:1177112/1‑60 (MQ=255) aaTTTTCGAAGCATCAATGGCATAACGCAGATCATGCCCCGGACGGTCAGCGACAAAGGt < 1:1884875/60‑1 (MQ=255) aaTTTTCGAAGCATCAATGGCATAACGCAGATCATGCCCCGGACGGTCAGCGACAAAGGt > 1:1660492/1‑60 (MQ=255) aaTTTTCGAAGCATCAATGGCATAACGCAGATCATGCCCCGGACGGTCAGCGACAAAGGt < 1:605739/60‑1 (MQ=255) aaTTTTCGAAGCATCAATGGCATAACGCAGATCATGCCCCGGACGGTCAGCGACAAAGGt < 1:1360250/60‑1 (MQ=255) aaTTTTCGAAGCATCAATGGCATAACGCAGATCATGCCCCGGACGGTCAGCGACAAAGGt < 1:588489/60‑1 (MQ=255) aaTTTTCGAAGCATCAATGGCATAACGCAGATCATGCCCCGGACGGTCAGCGACAAAGGt < 1:3148413/60‑1 (MQ=255) aaTTTTCGAAGCATCAATGGCATAACGCAGATCATGCAACGGACGGTCAGCGACAAAGGt > 1:1484186/1‑60 (MQ=255) aaTTTTCGAAGCATCAATGGCATAACGAAGATCATGCCCCGGACGGTCAGCGACAAAGGt > 1:1042511/1‑60 (MQ=255) aaTTTTCGAAGCATCAATGCCATAACGCAGATCATGCCCCGGACGGTCAGCGACa > 1:1213317/1‑55 (MQ=255) aaTTTTCCAAGCATCAATGGCATAACGCAGATCATGCCCCGGACGGTCAGCGACAAAGGt > 1:3379313/1‑60 (MQ=255) aaGCATCAATGGCATAACGCAGATCATGCCCCGGACGGTCAGCGACAAAGGTGATCAGGTCACGATAg < 1:1833509/68‑1 (MQ=255) aaGCATCAATGGCATAACGCAGATCATGCCCCGGACGGTCAGCGACAAAGGTGATCAGGTCACGATAg < 1:2096901/68‑1 (MQ=255) aaGCATCAATGGCATAACGCAGATCATGCCCCGGACGGTCAGCGACAAAGGTGATCAGGTCACGATAg < 1:2100792/68‑1 (MQ=255) tCAATGGCATAGCGTAGGTCATGCCCTGGACGGTCAGCGACAAAGGTGATCAGATCGCGATAGTGCGcc < 1:964384/69‑1 (MQ=255) tCAATGGCATAGCGTAGGTCATGCCCTGGACGGTCAGCGACAAAGGTGATCAGATCGCGATAGTGCGcc < 1:2733188/69‑1 (MQ=255) tCAATGGCATAGCGTAGGTCATGCCCTGGACGGTCAGCGACAAAGGTGATCAGATCGCGATAGTGCGcc < 1:3325217/69‑1 (MQ=255) tCAATGGCATAGCGTAGGTCATGCCCTGGACGGTCAGCGACAAAGGTGATCAGATCGCGATAGTGCGcc < 1:1850690/69‑1 (MQ=255) tCAATGGCATAGCGTAGGTCATGCCCTGGACGGTCAGCGACAAAGGTGATCAGATCGCGATAGTGCGcc < 1:1246653/69‑1 (MQ=255) tCAATGGCATAGCGTAGGTCATGCCCTGGACGGTCAGCGACAAAGGTGATCAGATCGCGATAGTGCGcc < 1:1057565/69‑1 (MQ=255) tCAATGGCATAGCGTAGGTCATGCCCTGGACGGTCAGCGACAAAGGTGATCAGATCGCGATAGTGCGcc < 1:3092678/69‑1 (MQ=255) tCAATGGCATAGCGTAGGTCATGCCCTGGACGGTCAGCGACAAAGGTGATCAGATCGCGATAGTGCGcc < 1:23131/69‑1 (MQ=255) tCAATGGCATAGCGTAGGTCATGCCCTGGACGGTCAGCGACAAAGGTGATCAGATCGCGATAGTGCGcc < 1:2322592/69‑1 (MQ=255) tCAATGGCATAGCGTAGGTCATGCCCTGGACGGTCAGCGACAAAGGTGATCAGATCGCGATAGTGCGcc < 1:2980844/69‑1 (MQ=255) tCAATGGCATAGCGTAGGTCATGCCCTGGACGGTCAGCGACAAAGGTGATCAGATCGCGATAGTGCGcc < 1:2620529/69‑1 (MQ=255) cAATGGCATAGCGTAGGTCATGCCCTGGACGGTCAGCGACAAAGGTGATCAGATCGCGATAGTGCGcc < 1:579176/68‑1 (MQ=255) aaTGGCATAGCGTAGGTCATGCCCTGGACGGTCAGCGACAAAGGTGATCAGATCGCGATAGTGCGcc < 1:2880467/67‑1 (MQ=255) aaTGGCATAACGCAGATCATGCCCCGGACGGTCAGCGACAAAGGTGATCAGGTCACGATAGTGCGCCAc > 1:1118714/1‑69 (MQ=255) aaTGGCATAACGCAGATCATGCCCCGGACGGTCAGCGACAAAGGTGATCAGGTCACGATAGTGCGCCAc > 1:855390/1‑69 (MQ=255) aaTGGCATAACGCAGATCATGCCCCGGACGGTCAGCGACAAAGGTGATCAGGTCACGATAGTGCGCCAc > 1:386862/1‑69 (MQ=255) tGGCATAGCGTAGGTCATGCCCTGGACGGTCAGCGACAAAGGTGATCAGATCGCGATAGTGCGCCACGc > 1:853196/1‑69 (MQ=255) tGGCATAGCGTAGGTCATGCCCTGGACGGTCAGCGACAAAGGTGATCAGATCGCGATAGTGCGCCACGc > 1:877865/1‑69 (MQ=255) tGGCATAGCGTAGGTCATGCCCTGGACGGTCAGCGACAAAGGTGATCAGATCGCGATAGTGCGCCACGc > 1:1003508/1‑69 (MQ=255) tGGCATAGCGTAGGTCATGCCCTGGACGGTCAGCGACAAAGGTGATCAGATCGCGATAGTGCGCCACGc > 1:340253/1‑69 (MQ=255) tGGCATAGCGTAGGTCATGCCCTGGACGGTCAGCGACAAAGGTGATCAGATCGCGATAGTGCGCCACGc > 1:3325856/1‑69 (MQ=255) tGGCATAGCGTAGGTCATGCCCTGGACGGTCAGCGACAAAGGTGATCAGATCGCGATAGTGCGCCACGc > 1:2664793/1‑69 (MQ=255) tGGCATAGCGTAGGTCATGCCCTGGACGGTCAGCGACAAAGGTGATCAGATCGCGATAGTGCGCCACGc > 1:330932/1‑69 (MQ=255) tGGCATAGCGTAGGTCATGCCCTGGACGGTCAGCGACAAAGGTGATCAGATCGCGATAGTGCGCCACGc > 1:1963554/1‑69 (MQ=255) tGGCATAGCGTAGGTCATGCCCTGGACGGTCAGCGACAAAGGTGATCAGATCGCGATAGTGCGCCACGc > 1:2868515/1‑69 (MQ=255) tGGCATAGCGTAGGTCATGCCCTGGACGGTCAGCGACAAAGGTGATCAGATCGCGATAGTGCGCCACGc > 1:2978560/1‑69 (MQ=255) aCGCAGGTCATGCCCCGGACGGTCAGCGACAAAGGTGATCTGGTCACGATAGTGCGCCACGCCGTGCgg > 1:438073/1‑69 (MQ=255) aCGCAGATCATGCCCCGGGCGGTCAGCGACAAAGGTGATCAGGTCACGATAGAGCGCCACGCCGTGCgg > 1:911101/1‑69 (MQ=255) aCGCAGATCATGCCCCGGACGGTCAGCGACAAAGGTGATCAGGTCACGATa > 1:1406410/1‑51 (MQ=255) aCGCAGATCATGCCCCGGACGGTCAGCGACAAAGGTGATCAGGTCACGATAGTGCGCCACGCCGTGCgg > 1:2080346/1‑69 (MQ=255) aCGCAGATCATGCCCCGGACGGTCAGCGACAAAGGTGATCAGGTCACGATAGTGCGCCACGCCGTGCgg > 1:2661973/1‑69 (MQ=255) aCGCAGATCATGCCCCGGACGGTCAGCGACAAAGGTGATCAGGTCACGATAGTGCGCCACGCCGTGCgg > 1:937040/1‑69 (MQ=255) aCGCAGATCATGCCCCGGACGGTCAGCGACAAAGGTGATCAGGTCACGATAGTGCGCCACGCCGTGCgg > 1:2547414/1‑69 (MQ=255) aCGCAGATCATGCCCCGGACGGTCAGCGACAAAGGTGATCAGGTCACGATAGTGCGCCACGCCGTGCgg > 1:2201858/1‑69 (MQ=255) aCGCAGATCATGCCCCGGACGGTCAGCGACAAAGGTGATCAGGTCACGATAGTGCGCCACGCCGTGCgg > 1:2102754/1‑69 (MQ=255) aCGCAGATCATGCCCCGGACGGTCAGCGACAAAGGTGATCAGGTCACGATAGTGCGCCACGCCGTGCgg > 1:1413839/1‑69 (MQ=255) aCGCAGATCATGCCCCGGACGGTCAGCGACAAAGGTGATCAGGTCACGATAGTGCGCCACGCCGTGCgg > 1:662708/1‑69 (MQ=255) aCGCAGATCATGCCCCGGACGGTCAGCGACAAAGGTGATCAGGTCACGATAGTGCGCCACGCCGTGCgg > 1:1337762/1‑69 (MQ=255) aCGCAGATCATGCCCCGGACGGTCAGCGACAAAGGTGATCAGGTCACGATAGTGCGCCACGCCGTGCgg > 1:1385443/1‑69 (MQ=255) aCGCAGATCATGCCCCGGACGGTCAGCGACAAAGGTGATCAGGTCACGATAGTGCGCCACGCCGTGCgg > 1:500377/1‑69 (MQ=255) aCGCAGATCATGCCCCGGACGGTCAGCGACAAAGGTGATCAGGTCACGATAGTGCGCCACGCCGTGCgg > 1:3333551/1‑69 (MQ=255) aCGCAGATCATGCCCCGGACGGTCAGCGACAAAGGTGATCAGGTCACGATAGTGCGCCACGCAGTGCgg > 1:2096719/1‑69 (MQ=255) | AAAGGTTTCCTGCGGCAGCCAGCCAAGTTCACGGGCAATTTTCGAAGCATCAATGGCATAACGCAGATCATGCCCCGGACGGTCAGCGACAAAGGTGATCAGGTCACGATAGTGCGCCACGCCGTGCGG > minE/2425132‑2425260 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |