| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,426,748 | G→T | 53.7% | V202V (GTC→GTA) | rffD ← | UDP‑N‑acetyl‑D‑mannosaminuronic acid dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,426,748 | 0 | G | T | 53.7% | ‑1.0 / 94.0 | 80 | V202V (GTC→GTA) | rffD | UDP‑N‑acetyl‑D‑mannosaminuronic acid dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base G (23/14); new base T (23/20); total (46/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.00e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AAAGCGATATTCACATCGCGGAAGCTGTTTTCGGTGAGCTTACACATTTCCGCCGTCCGCGAGTTAGTGACGACACACTCACCTTCGAGGAAAATTTTGTACAGTTCGCTGGCGCGGGCCGAACAAACCGGCGTCA > minE/2426680‑2426815 | aaaGCGATATTCACATCGCGGAAGCTGTTTTCGGTGAGCTTACGCATTTCCGCCGTCCGCGAGTTAGTg < 1:486532/69‑1 (MQ=255)aaaGCGATATTCACATCGCGGAAGCTGTTTTCGGTGAGCTTACACATTTCCGCCGTCCGCGAGTTAGTg < 1:2939573/69‑1 (MQ=255)aaaGCGATATTCACATCGCGGAAGCTGTTTTCGGTGAGCTTACACATTTCCGCCGTCCGCGAGTTAGTg < 1:1194890/69‑1 (MQ=255) tatTCACATCGCGGAAGCTGTTTTCGGTGAGCTTACACATTTCCGCCGTCCGCTAGTTAGTGACGacac < 1:2247128/69‑1 (MQ=255) tatTCACATCGCGGAAGCTGTTTTCGGTGAGCTTACACATTTCCGCCGTCCGCGAGTTAGTGACGacac < 1:1089064/69‑1 (MQ=255) tatTCACATCGCGGAAGCTGTTTTCGGTGAGCTTACACATTTCCGCCGTCCGCGAGTTAGTGACGacac < 1:3220461/69‑1 (MQ=255) tatTCACATCGCGGAAGCTGTTTTCGGTGAGCTTACACATTTCCGCCGTCCGCGAGTTAGTGACGacac < 1:1449380/69‑1 (MQ=255) atTCACATCGCGGAAGCTGTTTTCGGTGAGCTTACACATTTCCGCCGTCCGCGAGTTAGTGACGacaca > 1:3175733/1‑69 (MQ=255) atTCACATCGCGGAAGCTGTTTTCGGTGAGCTTACACATTTCCGCCGTCCGCGAGTTAGTGACGacaca > 1:1331753/1‑69 (MQ=255) aTCGCGGAAGCTGTTTTCGGTGAGCTTACACATTTCCGCCGTCCGCGAGTTAGTGACGACACACTCAcc < 1:1018041/69‑1 (MQ=255) gcgGAAGCTGTTTTCGGTGAGCTTACACATTTCCGCCGTCCGCGAGTTAGTGACGACACACTCAcc > 1:423131/1‑66 (MQ=255) gcgGAAGCTGTTTTCGGTGAGCTTACACATTTCCGCCGTCCGCGAGTTAGTGACGACACACTCACCt > 1:3129849/1‑67 (MQ=255) gcgGAAGCTGTTTTCGGTGAGCTTACACATTTCCGCCGTCCGCGAGTTAGTGACGACACACTCACCt > 1:2567484/1‑67 (MQ=255) gcgAAAGCTGTTTTCGGTGAGCTTACACATTTCCGCCGTCCGCGAGTTAGTTACGACACACTCACCTTc < 1:134930/69‑1 (MQ=255) gcgAAAGCTGTTTTCGGTGAGCTTACACATTTCCGCCGTCCGCGAGTTAGTTACGACACACTCACCTTc < 1:2422215/69‑1 (MQ=255) gcgAAAGCTGTTTTCGGTGAGCTTACACATTTCCGCCGTCCGCGAGTTAGTTACGACACACTCACCTTc < 1:2206865/69‑1 (MQ=255) gcgAAAGCTGTTTTCGGTGAGCTTACACATTTCCGCCGTCCGCGAGTTAGTTACGACACACTCACCTTc < 1:3174300/69‑1 (MQ=255) gcgAAAGCTGTTTTCGGTGAGCTTACACATTTCCGCCGTCCGCGAGTTAGTTACGACACACTCACCTTc < 1:2065018/69‑1 (MQ=255) gcgAAAGCTGTTTTCGGTGAGCTTACACATTTCCGCCGTCCGCGAGTTAGTTACGACACACTCACCTTc < 1:965287/69‑1 (MQ=255) gcgAAAGCTGTTTTCGGTGAGCTTACACATTTCCGCCGTCCGCGAGTTAGTTACGACACACTCACCTTc < 1:1917525/69‑1 (MQ=255) gcgAAAGCTGTTTTCGGTGAGCTTACACATTTCCGCCGTCCGCGAGTTAGTTACGACACACTCACCTTc < 1:2727738/69‑1 (MQ=255) gcgAAAGCTGTTTTCGGTGAGCTTACACATTTCCGCCGTCCGCGAGTTAGTTACGACACACTCACCTTc < 1:1849944/69‑1 (MQ=255) gcgAAAGCTGTTTTCGGTGAGCTTACACATTTCCGCCGTCCGCGAGTTAGTTACGACACACTCACCTTc < 1:842324/69‑1 (MQ=255) gcgAAAGCTGTTTTCGGTGAGCTTACACATTTCCGCCGTCCGCGAGTTAGTTACGACACACTCACCTTc < 1:2514059/69‑1 (MQ=255) gcgAAAGCTGTTTTCGGTGAGCTTACACATTTCCGCCGTCCGCGAGTTAGTTACGACACACTCACCTTc < 1:161028/69‑1 (MQ=255) gcgAAAGCTGTTTTCGGTGAGCTTACACATTTCCGCCGTCCGCGAGTTAGTTACGACACACTCACCTTc < 1:1602778/69‑1 (MQ=255) gcgAAAGCTGTTTTCGGTGAGCTTACACATTTCCGCCGTCCGCGAGTTAGTTACGACACACTCACCTTc < 1:1520799/69‑1 (MQ=255) gcgAAAGCTGTTTTCGGTGAGCTTACACATTTCCGCCGTCCGCGAGTTAGTTACGACACACTCACCTTc < 1:724267/69‑1 (MQ=255) gcgAAAGCTGTTTTCGGTGAGCTTACACATTTCCGCCGTCCGCGAGTTAGTTACGACACACTCACCTTc < 1:1375217/69‑1 (MQ=255) gcgAAAGCTGTTTTCGGTGAGCTTACACATTTCCGCCGTCCGCGAGTTAGTTACGACACACTCACCTTc < 1:599854/69‑1 (MQ=255) cgaaaGCTGTTTTCGGTGAGCTTACACATTTCCGCCGTCCGCGAGTTAGTTACGACACACTCACCTTc < 1:1616904/65‑1 (MQ=255) cgaaaGCTGTTTTCGGTGAGCTTACACATTTCCGCCGTCCGCGAGTTAGTTACGACACACTCACCTTc < 1:1336414/65‑1 (MQ=255) aGCTGTTTTCGGTGAGCTTACACATTTCCGCCGTCCGCGAGTTAGTGACGACACACTCACCTTCGAgg > 1:3028332/1‑68 (MQ=255) cTTACACATTTCCGCCGTCCGCGAGTTAGTGACGACACACTCACCTTCGAGGAAAATTTTGTACAGTTc < 1:484296/69‑1 (MQ=255) cTTACACATTTCCGCCGTCCGCGAGTTAGTGACGACACACTCACCTTCGAGGAAAATTTTGTACAGTTc < 1:626522/69‑1 (MQ=255) cTTACACATTTCCGCCGTCCGCGAGTTAGTGACGACACACTCACCTTCGAGGAAAATTTTGTACAGTTc < 1:2284286/69‑1 (MQ=255) cTTACACATTTCCGCCGTCCGCGAGTTAGTGACGACACACTCACCTTCGACGAAAATTTTGTACAGTTc < 1:1405791/69‑1 (MQ=255) ttACACATTTCCGCCGTCCGCGAGTTAGTTACGAGACACTCACCTTc < 1:2939328/47‑1 (MQ=255) cgcgAGTTAGTGACGACACACTCACCTTCGAGGAAAATTTTGTACAGTTCGCTGGCGCGGGCCGAACaa < 1:2369394/69‑1 (MQ=255) cgcgAGTTAGTGACGACACACTCACCTTCGAGGAAAATTTTGTACAGTTCGCTGGCGCGGGCCGAACaa < 1:172818/69‑1 (MQ=255) cgcgAGTTAGTGACGACACACTCACCTTCGAGGAAAATTTTGTACAGTTCGCTGGCGCGGGCCGAACaa < 1:3192520/69‑1 (MQ=255) cgcgAGTTAGTGACGACACACTCACCTTCGAGGAAAATTTTGTACAGTTCGCTGGCGCGGGCCGAACaa < 1:939963/69‑1 (MQ=255) cgAGTTAGTTACGACACACTCACCTTCGAGGAAAATTTTGTACAGTTCGCTGGCGCGGGCCGAACa > 1:3036938/1‑66 (MQ=255) cgAGTTAGTTACGACACACTCACCTTCGAGGAAAATTTTGTACAGTTCGCTGGCGCGGGCCGAACa > 1:3054042/1‑66 (MQ=255) cgAGTTAGTTACGACACACTCACCTTCGAGGAAAATTTTGTACAGTTCGCTGGCGCGGGCCGAACa > 1:3094785/1‑66 (MQ=255) cgAGTTAGTTACGACACACTCACCTTCGAGGAAAATTTTGTACAGTTCGCTGGCGCGGGCCGAACa > 1:3107999/1‑66 (MQ=255) cgAGTTAGTTACGACACACTCACCTTCGAGGAAAATTTTGTACAGTTCGCTGGCGCGGGCCGAACa > 1:3153833/1‑66 (MQ=255) cgAGTTAGTTACGACACACTCACCTTCGAGGAAAATTTTGTACAGTTCGCTGGCGCGGGCCGAACa > 1:990370/1‑66 (MQ=255) cgAGTTAGTTACGACACACTCACCTTCGAGGAAAATTTTGTACAGTTCGCTGGCGCGGGCCGAACa > 1:3404658/1‑66 (MQ=255) cgAGTTAGTTACGACACACTCACCTTCGAGGAAAATTTTGTACAGTTCGCTGGCGCGGGCCGAACa > 1:483570/1‑66 (MQ=255) cgAGTTAGTTACGACACACTCACCTTCGAGGAAAATTTTGTACAGTTCGCTGGCGCGGGCCGAACa > 1:2873512/1‑66 (MQ=255) cgAGTTAGTTACGACACACTCACCTTCGAGGAAAATTTTGTACAGTTCGCTGGCGCGGGCCGAACa > 1:265363/1‑66 (MQ=255) cgAGTTAGTTACGACACACTCACCTTCGAGGAAAATTTTGTACAGTTCGCTGGCGCGGGCCGAACa > 1:1034526/1‑66 (MQ=255) cgAGTTAGTTACGACACACTCACCTTCGAGGAAAATTTTGTACAGTTCGCTGGCGCGGGCCGAACa > 1:1062167/1‑66 (MQ=255) cgAGTTAGTTACGACACACTCACCTTCGAGGAAAATTTTGTACAGTTCGCTGGCGCGGGCCGAACa > 1:1110499/1‑66 (MQ=255) cgAGTTAGTTACGACACACTCACCTTCGAGGAAAATTTTGTACAGTTCGCTGGCGCGGGCCGAACa > 1:1160303/1‑66 (MQ=255) cgAGTTAGTTACGACACACTCACCTTCGAGGAAAATTTTGTACAGTTCGCTGGCGCGGGCCGAACa > 1:1378194/1‑66 (MQ=255) cgAGTTAGTTACGACACACTCACCTTCGAGGAAAATTTTGTACAGTTCGCTGGCGCGGGCCGAACa > 1:1574166/1‑66 (MQ=255) cgAGTTAGTTACGACACACTCACCTTCGAGGAAAATTTTGTACAGTTCGCTGGCGCGGGCCGAACa > 1:1617646/1‑66 (MQ=255) cgAGTTAGTTACGACACACTCACCTTCGAGGAAAATTTTGTACAGTTCGCTGGCGCGGGCCGAACa > 1:1868304/1‑66 (MQ=255) cgAGTTAGTTACGACACACTCACCTTCGAGGAAAATTTTGTACAGTTCGCTGGCGCGGGCCGAACa > 1:2163195/1‑66 (MQ=255) cgAGTTAGTTACGACACACTCACCTTCGAGGAAAATTTTGTACAGTTCGCTGGCGCGGGCCGAACa > 1:2205099/1‑66 (MQ=255) cgAGTTAGTTACGACACACTCACCTTCGAGGAAAATTTTGTACAGTTCGCTGGCGCGGGCCGAACa > 1:2458715/1‑66 (MQ=255) cgAGTTAGTTACGACACACTCACCTTCGAGGAAAATTTTGTACAGTTCGCTGGCGCGGGCCGAACa > 1:2244338/1‑66 (MQ=255) cgAGTTAGTTACGACACACTCACCTTCGAGGAAAACTTTGTACAGTTCGCTGGCGCGGGCCGAACa > 1:1775029/1‑66 (MQ=255) cgAGTTAGTGACGACACACTCACCTTCGAGGAAAATTTTGTACAGTTCGCTGGCGCGGGCCGAACaa > 1:2220905/1‑67 (MQ=255) cgAGTTAGTGACGACACACTCACCTTCGAGGAAAATTTTGTACAGTTCGCTGGCGCGGGCCGAACaa > 1:3204896/1‑67 (MQ=255) cgAGTTAGTGACGACACACTCACCTTCGAGGAAAATTTTGTACAGTTCGCTGGCGCGGGCCGAACaa > 1:1974909/1‑67 (MQ=255) cgAGTTAGTGACGACACACTCACCTTCGAGGAAAATTTTGTACAGTTCGCTGGCGCGGGCCGAACaa > 1:3225725/1‑67 (MQ=255) cgAGTTAGTGACGACACACTCACCTTCGAGGAAAATTTTGTACAGTTCGCTGGCGCGGGCCGAACaa > 1:2460991/1‑67 (MQ=255) cgAGTTAGTGACGACACACTCACCTTCGAGGAAAATTTTGTACAGTTCGCTGGCGCGGGCCGAACaa > 1:3385655/1‑67 (MQ=255) cgAGTTAGTGACGACACACTCACCTTCGAGGAAAATTTTGTACAGTTCGCTGGCGCGGGCCGAACaa > 1:954798/1‑67 (MQ=255) cgAGTTAGTGACGACACACTCACCTTCGAGGAAAATTTTGTACAGTTCGCTGGCGCGGGCCGAACaa < 1:941785/67‑1 (MQ=255) cgAGTTAGTGACGACACACTCACCTTCGAGGAAAATTTTGTACAGTTCGCTGGCGCGGGCCGAACaa > 1:3087490/1‑67 (MQ=255) cgAGTTAGTGACGACACACTCACCTTCGAGGAAAATTTTGTACAGTTCGCTGGCGCGGGCCGAACaa > 1:860212/1‑67 (MQ=255) cgAGTTAGTGACGACACACTCACCTTCGAGGAAAATTTTGTACAGTTCGCTGGCGCGGGCCGAACAAAc > 1:2794998/1‑69 (MQ=255) cgAGTTAGTGACGACACACTCACCTTCGAGGAAAATTTTGTACAGTTCGCTGGCGCGGGCCGAACAAAc > 1:3152852/1‑69 (MQ=255) cgAGTTAGTGACGACACACTCACCTTCGAGGAAAATTTTGTACAGTTCGCTGGCGCGGGCCGAACAAAc > 1:2258602/1‑69 (MQ=255) cgAGTTAGTGACGACACACTCACCTTCGAGGAAAATTTTGTACAGTTCGCTGGCGCGGGCCGAACAAAc > 1:828821/1‑69 (MQ=255) cgAGTTAGTGACGACACACTCACCTTCGAGGAAAATTTTGTACAGTTCGCTGGCGCGGGCCGAACAAAc > 1:705226/1‑69 (MQ=255) cgAGTTAGTGACGACACACTCACCTTCGAGGAAAATTTTGTACAGTTCGCTGGCGCGGGCCGAACAAAc > 1:136367/1‑69 (MQ=255) cgAGTTAGTGACGACACACTCACCTTCGAGGAAAATTTTGTACAGTTCGCTGGCGCGGGCCGAACAAAc > 1:54535/1‑69 (MQ=255) cgAGTTAGTGACGACACACTCACCTTCGAGGAAAATTTTGTACAGTTCGCTGGCGCGGGCCGAACAAAc > 1:3287187/1‑69 (MQ=255) gacgacACACTCACCTTCGAGGAAAATTTTGTACAGTTCGCTGGCGTGGGCCGAACaa > 1:1468462/1‑58 (MQ=255) gacgacACACTCACCTTCGAGGAAAATTTTGTACAGTTCGCTGGCGCGGGCCGAACaa > 1:1343999/1‑58 (MQ=255) gacgacACACTCACCTTCGAGGAAAATTTTGTACAGTTCGCTGGCGCGGGCCGAACaa > 1:126554/1‑58 (MQ=255) gacgacACACTCACCTTCGAGGAAAATTTTGTACAGTTCGCTGGCGCGGGCCGAACaa > 1:79969/1‑58 (MQ=255) gacgacACACTCACCTTCGAGGAAAATTTTGTACAGTTCGCTGGCGCGGGCCGAACaa > 1:2228241/1‑58 (MQ=255) gacgacACACTCACCTTCGAGGAAAATTTTGTACAGTTCGCTGGCGCGGGCCGAACAAACCGGCGTCa < 1:85962/68‑1 (MQ=255) gacgacACACTCACCTTCGAGGAAAATTTTGTACAGTTCGCTGGCGCGGGCCGAACAAACCGGCGTCa < 1:2145502/68‑1 (MQ=255) gacgacACACTCACCTTCGAGGAAAATTTTGTACAGTTCGCTGGCGCGGGCCGAACAAACCGGCGTCa < 1:3215212/68‑1 (MQ=255) gacgacACACTCACCTTCGAGGAAAATTTTGTACAGTTCGCTGGCGCGGGCCGAACAAACCGACGTCa < 1:1622536/68‑1 (MQ=255) | AAAGCGATATTCACATCGCGGAAGCTGTTTTCGGTGAGCTTACACATTTCCGCCGTCCGCGAGTTAGTGACGACACACTCACCTTCGAGGAAAATTTTGTACAGTTCGCTGGCGCGGGCCGAACAAACCGGCGTCA > minE/2426680‑2426815 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |