| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,428,430 | C→T | 35.8% | A17A (GCG→GCA) | rffE ← | UDP‑N‑acetyl glucosamine‑2‑epimerase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,428,430 | 0 | C | T | 35.8% | 70.4 / 61.4 | 103 | A17A (GCG→GCA) | rffE | UDP‑N‑acetyl glucosamine‑2‑epimerase |
| Reads supporting (aligned to +/- strand): ref base C (25/41); new base T (17/20); total (42/61) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.31e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 103 total aligned reads displayed.
TGCTGCGCAGTGACGCAAACTTTAGCCTCAAAAAAAGGATCTTTTGCCAACGCATGCACCAACGGCGCCATCTTGATGGCTTCCGGGCGCGTACCAAATACAGTCAGTACTTTCACATCGATTCTCTT > minE/2428365‑2428492 | tgctgcGCAGTGACGCAAACTTTAGCCTCAAAAAAAGGATCTTTTGCCAACGCATGCACCAACGGCGcc < 1:2176683/69‑1 (MQ=255)tgctgcGCAGTGACGCAAACTTTAGCCTCAAAAAAAGGATCTTTTGCCAACGCATGCACCAACGGCGcc < 1:1157413/69‑1 (MQ=255)tgctgcGCAGTGACGCAAACTTTAGCCTCAAAAAAAGGATCTTTTGCCAACGCATGCACCAACGGCGcc < 1:2760505/69‑1 (MQ=255)tgctgcGCAGTGACGCAAACTTTAGCCTCAAAAAAAGGATCTTTTGCCAACGCATGCACCAACGGCGcc < 1:2756353/69‑1 (MQ=255)tgctgcGCAGTGACGCAAACTTTAGCCTCAAAAAAAGGATCTTTTGCCAACGCATGCACCAACGGCGcc < 1:159554/69‑1 (MQ=255) gctgcGCAGTGACGCAAACTTTAGCCTCAAAAAAAGGATCTTTTGCCAACGCATGCACCAACGGCGcc < 1:51616/68‑1 (MQ=255) ctgcGCAGTGACGCAAACTTTAGCCTCAAAAAAAGGATCTTTTGCCAACGCATGCACCAACGGCGCCAt > 1:2212879/1‑69 (MQ=255) ctgcGCAGTGACGCAAACTTTAGCCTCAAAAAAAGGATCTTTTGCCAACGCATGCACCAACGGCGCCAt > 1:3161472/1‑69 (MQ=255) ctgcGCAGTGACGCAAACTTTAGCCTCAAAAAAAGGATCTTTTGCCAACGCATGCACCAACGGCGCCAt > 1:323552/1‑69 (MQ=255) cAGTGACGCAAACTTTAGCCTCAAAAAAAGGATCTTTTGCCAACGCATGCACCAACTGCGCCATCtt < 1:1777005/67‑1 (MQ=255) cAGTGACGCAAACTTTAGCCTCAAAAAAAGGATCTTTTGCCAACGCATGCACCAACGGCGCCATCtt < 1:2750344/67‑1 (MQ=255) cAGTGACGCAAACTTTAGCCTCAAAAAAAGGATCTTTTGCCAACGCATGCACCAACGGCGCCATCtt < 1:1067181/67‑1 (MQ=255) cAGTGACGCAAACTTTAGCCTCAAAAAAAGGATCTTTTGCCAACGCATGCACCAACGGCGCCATCtt < 1:1951925/67‑1 (MQ=255) cAGTGACGCAAACTTTAGCCTCAAAAAAAGGATCTTTTGCCAACGCATGCACCAACGGCGCCATCtt < 1:2603943/67‑1 (MQ=255) cAGTGACGCAAACTTTAGCCTCAAAAAAAGGATCTTTTGCCAACGCATGCACCAACGGCGCCATCtt < 1:2493944/67‑1 (MQ=255) cAGTGACGCAAACTTTAGCCTCAAAAAAAGGATCTTTTGCCAACGCATGCACCAACGGCGCCATCtt < 1:239166/67‑1 (MQ=255) cAGTGACGCAAACTTTAGCCTCAAAAAAAGGATCTTTTGCCAACGCATGCACCAACGGCGCCATCtt < 1:3100443/67‑1 (MQ=255) cAGTGACGCAAACTTTAGCCTCAAAAAAAGGATCTTTTGCCAACGCATGCACCAACGGCGCCATCtt < 1:2874521/67‑1 (MQ=255) cAGTGACGCAAACTTTAGCCTCAAAAAAAGGATCTTTTGCCAACGCATGCACCAACGGCGCCATCtt < 1:3011713/67‑1 (MQ=255) cAGTGACGCAAACTTTAGCCTCAAAAAAAGGATCTTTTGCCAACGCATGCACCAACGGCGCCATCtt < 1:3333727/67‑1 (MQ=255) cAGTGACGCAAACTTTAGCCTCAAAAAAAGGATCTTTTGCCAACGCATGCACCAACGGCGCCATCtt < 1:356076/67‑1 (MQ=255) cAGTGACGCAAACTTTAGCCTCAAAAAAAGGATCTTTTGCCAACGCATGCACCAACGGCGCCATCtt < 1:486791/67‑1 (MQ=255) cAGTGACGCAAACTTTAGCCTCAAAAAAAGGATCTTTTGCCAACGCATGCACCAACGGCGCCATCtt < 1:1252640/67‑1 (MQ=255) cAGTGACGCAAACTTTAGCCTCAAAAAAAGGATCTTTTGCCAACGCATGCACCAACGGCGCCATCtt < 1:846045/67‑1 (MQ=255) cAGTGACGCAAACTTTAGCCTCAAAAAAAGGATCTTTTGCCAACGCATGCACCAACGGCGCCATCtt < 1:1217014/67‑1 (MQ=255) cAGTGACGCAAACTTTAGCCTCAAAAAAAGGATCTTTTGCCAACGCATGCACCAACGGCGCCATCtt < 1:1096646/67‑1 (MQ=255) cAGTGACGCAAACTTTAGCCTCAAAAAAAGGATCTTTTGCCAACGCATGCACCAACGGCGCCATCtt < 1:106719/67‑1 (MQ=255) aGTGACGCAAACTTTAGCCTCAAAAAAAGGATCTTTTGCCAACGCATGCACCAACGGCGCCATCtt < 1:2690510/66‑1 (MQ=255) gACGCAAACTTTAGCCTCTAAAAAAGGATCTTTTGCCAACGCATGCACCAACGGCGCCATCTTGATGGc > 1:2690547/1‑69 (MQ=255) gACGCAAACTTTAGCCTCAAAAAAAGGATCTTTTGCCAACGCATGCACCAACGGTGCCATCTTGATGGc > 1:2009179/1‑69 (MQ=255) gACGCAAACTTTAGCCTCAAAAAAAGGATCTTTTGCCAACGCATGCACCAACGGTGCCATCTTGATGGc > 1:1675666/1‑69 (MQ=255) gACGCAAACTTTAGCCTCAAAAAAAGGATCTTTTGCCAACGCATGCACCAACGGTGCCATCTTGATGGc > 1:1640728/1‑69 (MQ=255) gACGCAAACTTTAGCCTCAAAAAAAGGATCTTTTGCCAACGCATGCACCAACGGTGCCATCTTGATGGc > 1:1968937/1‑69 (MQ=255) gACGCAAACTTTAGCCTCAAAAAAAGGATCTTTTGCCAACGCATGCACCAACGGTGCCATCTTGATGGc > 1:2513561/1‑69 (MQ=255) gACGCAAACTTTAGCCTCAAAAAAAGGATCTTTTGCCAACGCATGCACCAACGGTGCCATCTTGATGGc > 1:1984969/1‑69 (MQ=255) gACGCAAACTTTAGCCTCAAAAAAAGGATCTTTTGCCAACGCATGCACCAACGGTGCCATCTTGATGGc > 1:1630472/1‑69 (MQ=255) gACGCAAACTTTAGCCTCAAAAAAAGGATCTTTTGCCAACGCATGCACCAACGGTGCCATCTTGATGGc > 1:2934224/1‑69 (MQ=255) gACGCAAACTTTAGCCTCAAAAAAAGGATCTTTTGCCAACGCATGCACCAACGGTGCCATCTTGATGGc > 1:2480760/1‑69 (MQ=255) gACGCAAACTTTAGCCTCAAAAAAAGGATCTTTTGCCAACGCATGCACCAACGGTGCCATCTTGATGGc > 1:1444089/1‑69 (MQ=255) gACGCAAACTTTAGCCTCAAAAAAAGGATCTTTTGCCAACGCATGCACCAACGGTGCCATCTTGATGGc > 1:2368368/1‑69 (MQ=255) gACGCAAACTTTAGCCTCAAAAAAAGGATCTTTTGCCAACGCATGCACCAACGGTGCCATCTTGATGGc > 1:64876/1‑69 (MQ=255) gACGCAAACTTTAGCCTCAAAAAAAGGATCTTTTGCCAACGCATGCACCAACGGTGCCATCTTGATGGc > 1:767938/1‑69 (MQ=255) gACGCAAACTTTAGCCTCAAAAAAAGGATCTTTTGCCAACGCATGCACCAACGGTGCCATCTTGATGGc > 1:2182244/1‑69 (MQ=255) gACGCAAACTTTAGCCTCAAAAAAAGGATCTTTTGCCAACGCATGCACCAACGGTGCCATCTTGATGGc > 1:2803249/1‑69 (MQ=255) gACGCAAACTTTAGCCTCAAAAAAAGGATCTTTTGCCAACGCATGCACCAACGGTGCCATCTTGATGGc > 1:2170230/1‑69 (MQ=255) gACGCAAACTTTAGCCTCAAAAAAAGGATCTTTTGCCAACGCATGCACCAACGGTGCCATATTGATGGc > 1:594644/1‑69 (MQ=255) gACGCAAACTTTAGCCTCAAAAAAAGGATCTTTTGCCAACGCATGCACCAACGGCGCCATCtt > 1:288964/1‑63 (MQ=255) gACGCAAACTTTAGCCTCAAAAAAAGGATCTTTTGCCAACGCATGCACCAACGGCGCCATCtt > 1:1921923/1‑63 (MQ=255) gACGCAAACTTTAGCCTCAAAAAAAGGATCTTTTGCCAACGCATGCACCAACGGCGCCATCtt > 1:2162607/1‑63 (MQ=255) gACGCAAACTTTAGCCTCAAAAAAAGGATCTTTTGCCAACGCATGCACCAACGGCGCCATCtt > 1:3163440/1‑63 (MQ=255) gACGCAAACTTTAGCCTCAAAAAAAGGATCTTTTGCCAACGCATGCACCAACGGCGCCATCtt > 1:527258/1‑63 (MQ=255) gACGCAAACTTTAGCCTCAAAAAAAGGATCTTTTGCCAACGCATGCACCAACGGCGCCATCtt > 1:656417/1‑63 (MQ=255) gACGCAAACTTTAGCCTCAAAAAAAGGATCTTTTGCCAACGCATGCACCAACGGCGCCATCtt > 1:843464/1‑63 (MQ=255) gACGCAAACTTTAGCCTCAAAAAAAGGATCTTTTGCCAACGCATGCACCAACGGCGCCATCtt > 1:883178/1‑63 (MQ=255) gACGCAAACTTTAGCCTCAAAAAAAGGATCTTTTGCCAACGCATGCACCAACGGCGCCATCtt > 1:2675232/1‑63 (MQ=255) aCGCAAACTTTAGCCTCAAAAAAAGTATCTTTTGCCAACGCATGCACCAACGGCGCCATCTTGATGGCt > 1:1468573/1‑69 (MQ=255) aCGCAAACTTTAGCCTCAAAAAAAGGATCTTTTGCCAACGCATGCACCAACGGCGCCATCTTGATGGCt < 1:2241851/69‑1 (MQ=255) aCGCAAACTTTAGCCTCAAAAAAAGGATCTTTTGCCAACGCATGCACCAACGGCGCCATCTTGATGGCt < 1:2141046/69‑1 (MQ=255) aCGCAAACTTTAGCCTCAAAAAAAGGATCTTTTGCCAACGCATGCACCAACGGCGCCATCTTGATGGCt > 1:2342375/1‑69 (MQ=255) aCGCAAACTTTAGCCTCAAAAAAAGGATCTTTTGCCAACGCATGCACCAACGGCGCCATCTTGATGGCt < 1:139221/69‑1 (MQ=255) aCGCAAACTTTAGCCTCAAAAAAAGGATCTTTTGCCAACGCATGCACCAACGGCGCCATCTTGATGGCt > 1:2899054/1‑69 (MQ=255) aCGCAAACTTTAGCCTCAAAAAAAGGATCTTTTGCCAACGCATGCACCAACGGCGCCATCTTGATGGCt > 1:1250355/1‑69 (MQ=255) aCGCAAACTTTAGCCTCAAAAAAAGGATCTTTTGCCAACGCATGCACCAACGGCGCCATCTTGATGGCt < 1:2641750/69‑1 (MQ=255) aCGCAAACTTTAGCCTCAAAAAAAGGATCTTTTGCCAACGCATGCACCAACGGCGCCATCTTGATGGCt < 1:3249934/69‑1 (MQ=255) aCGCAAACTTTAGCCTCAAAAAAAGGATCTTTTG‑CAACGCATGCACCAACGGTGCCATCTTGATGGCtt < 1:276662/69‑1 (MQ=255) gCAAACTTTAGCCTCAAAAAAAGGATCTTTTGCCAACGCATGCACCAACGGTGCCATCTTGATGGCtt < 1:1922313/68‑1 (MQ=255) gCAAACTTTAGCCTCAAAAAAAGGATCTTTTGCCAACGCATGCACCAACGGTGCCATCTTGATGGCtt < 1:2405436/68‑1 (MQ=255) gCAAACTTTAGCCTCAAAAAAAGGATCTTTTGCCAACGCATGCACCAACGGTGCCATCTTGATGGCtt < 1:436509/68‑1 (MQ=255) gCAAACTTTAGCCTCAAAAAAAGGATCTTTTGCCAACGCATGCACCAACGGTGCCATCTTGATGGCtt < 1:3396399/68‑1 (MQ=255) gCAAACTTTAGCCTCAAAAAAAGGATCTTTTGCCAACGCATGCACCAACGGTGCCATCTTGATGGCtt < 1:792106/68‑1 (MQ=255) gCAAACTTTAGCCTCAAAAAAAGGATCTTTTGCCAACGCATGCACCAACGGTGCCATCTTGATGGCtt < 1:850573/68‑1 (MQ=255) gCAAACTTTAGCCTCAAAAAAAGGATCTTTTGCCAACGCATGCACCAACGGTGCCATCTTGATGGCtt < 1:2085245/68‑1 (MQ=255) gCAAACTTTAGCCTCAAAAAAAGGATCTTTTGCCAACGCATGCACCAACGGTGCCATCTTGATGGCtt < 1:1927900/68‑1 (MQ=255) gCAAACTTTAGCCTCAAAAAAAGGAGCTTTTGCCAACGCATGCACCAACGGTGCCATCTTGATGGCtt < 1:2444449/68‑1 (MQ=255) cTTTAGCCTCAAAAAAAGGATCTTTTGCCAACGCATGCACCAACGGCGCCATCtt < 1:2862063/55‑1 (MQ=255) aaaaaGGATCTTTTGCCAACGCATGCACCAACGGCGCCATCTTGATGGCTTCCGGGCGCGTACCaaa < 1:1769376/67‑1 (MQ=255) ttttGCCAACGCATGCACCAACGGCGCCATCTTGATGGCTTCCGGGCGCGTACCaaa < 1:699705/57‑1 (MQ=255) gCATGCACCAACGGTGCCATCTTGATGGCTTCCGGGCGCGTACCAAATACAGTCAGTACTTTcaca < 1:2483290/66‑1 (MQ=255) gCATGCACCAACGGTGCCATCTTGATGGCTTCCGGGCGCGTACCAAATACAGTCAGTACTTTcaca < 1:2870447/66‑1 (MQ=255) gCATGCACCAACGGTGCCATCTTGATGGCTTCCGGGCGCGTACCAAATACAGTCAGTACTTTcaca < 1:452179/66‑1 (MQ=255) gCATGCACCAACGGTGCCATCTTGATGGCTTCCGGGCGCGTACCAAATACAGTCAGTACTTTcaca < 1:425453/66‑1 (MQ=255) gCATGCACCAACGGTGCCATCTTGATGGCTTCCGGGCGCGTACCAAATACAGTCAGTACTTTcaca < 1:3420340/66‑1 (MQ=255) gCATGCACCAACGGTGCCATCTTGATGGCTTCCGGGCGCGTACCAAATACAGTCAGTACTTTcaca < 1:2939015/66‑1 (MQ=255) gCATGCACCAACGGTGCCATCTTGATGGCTTCCGGGCGCGTACCAAATACAGTCAGTACTTTcaca < 1:72776/66‑1 (MQ=255) gCATGCACCAACGGTGCCATCTTGATGGCTTCCGGGCGCGTACCAAATACAGTCAGTACTTTcaca < 1:3321342/66‑1 (MQ=255) gCATGCACCAACGGTGCCATCTTGATGGCTTCCGGGCGCGTACCAAATACAGTCAGTACTTTcaca < 1:2067848/66‑1 (MQ=255) gCATGCACCAACGGTGCCATCTTGATGGCTTCCGGGCGCGTACCAAATACAGTCAGTACGTTcaca < 1:7626/66‑1 (MQ=255) tGCACCAACGGCGCCATCTTGATGGCTTCCGGGCGCGTACCAAATACAGTCAGTACTTTCACATCGAtt > 1:1830743/1‑69 (MQ=255) gCACCAACGGCGCCATCTTGATGGCTTCCGGGCGCGTACCAAATACAGTCAGTACTTTCACATCGATtc < 1:597965/69‑1 (MQ=255) gCACCAACGGCGCCATCTTGATGGCTTCCGGGCGCGTACCAAATACAGTCAGTACTTTCACATCGATtc < 1:1522271/69‑1 (MQ=255) gCACCAACGGCGCCATCTTGATGGCTTCCGGGCGCGTACCAAATACAGTCAGTACTTTCACATCGATtc < 1:312665/69‑1 (MQ=255) gCACCAACGGCGCCATCTTGATGGCTTCCGGGCGCGTACCAAATACAGTCAGTACTTTCACATCGATtc < 1:2737246/69‑1 (MQ=255) gCACCAACGGCGCCACCTTGATGGCTTCCGGGCGCGTACCAAATACAGTCAGTACTTTCACATCGATtc < 1:1554951/69‑1 (MQ=255) cACCAACGGCGCCATCTTGATGGCTTCCGGGCGCGTACCAAATACAGTCAGTACTTTCACATCGATtct > 1:2828643/1‑69 (MQ=255) cACCAACGGCGCCATCTTGATGGCTTCCGGGCGCGTACCAAATACAGTCAGTACTTTCACATCGATtc < 1:2646720/68‑1 (MQ=255) aaCGGCGCCATCTTGATGGCTTCCGGGCGCGTACCAAATAcagtc > 1:2335086/1‑45 (MQ=255) aaCGGCGCCATCTTGATGGCTTCCGGGCGCGTACCAAATAcagtc > 1:481066/1‑45 (MQ=255) aaCGGCGCCATCTTGATGGCTTCCGGGCGCGTACCAAATAcagtc > 1:3197136/1‑45 (MQ=255) aaCGGCGCCATCTTGATGGCTTCCGGGCGCGTACCAAATAcagtc > 1:968688/1‑45 (MQ=255) aaCGGCGCCATCTTGATGGCTTCCGGGCGCGTACCAAATACAGTCAGTACTTTCACATCGATTCTCtt > 1:2485462/1‑68 (MQ=255) | TGCTGCGCAGTGACGCAAACTTTAGCCTCAAAAAAAGGATCTTTTGCCAACGCATGCACCAACGGCGCCATCTTGATGGCTTCCGGGCGCGTACCAAATACAGTCAGTACTTTCACATCGATTCTCTT > minE/2428365‑2428492 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |