| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 27,559 | A→C | 36.5% | A77A (GCA→GCC) | carA → | carbamoyl phosphate synthetase small subunit, glutamine amidotransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 27,559 | 0 | A | C | 36.5% | 73.8 / 111.7 | 126 | A77A (GCA→GCC) | carA | carbamoyl phosphate synthetase small subunit, glutamine amidotransferase |
| Reads supporting (aligned to +/- strand): ref base A (50/30); new base C (21/25); total (71/55) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.28e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.77e-01 | |||||||||||
Only 100 of 147 total aligned reads displayed.
ACTTATCCCCATATTGGCAATGTCGGCACCAATGACGCCGATGAAGAATCTTCTCAGGTACATGCACAAGGTCTGGTGATTCGCGACCTGCCGCTGATTGCCAGCAACTTCCGTAATACCGAAGACCT > minE/27494‑27621 | acttaTCCCCATATTGGCAATGTCGGCACCAATGACGCCGATGAAGAATCTTCTCAGGTACATGcaca > 1:3356755/1‑68 (MQ=255)acttaTCCCCATATTGGCAATGTCGGCACCAATGACGCCGATGAAGAATCTTCTCAGGTACATGCACaa > 1:1024426/1‑69 (MQ=255) cATATTGGCAATTTCGGCACTAATGACGCCGATGAAGAATCTTCTCAGGTTCATGCCCAAGGTCTGGt < 1:1455524/68‑1 (MQ=255) cATATTGGCAATGTCGGCACTAATGACGCCGATGAAGAATCTTCTCAGGTTCATGCCCAAGGTCTGGt < 1:810251/68‑1 (MQ=255) cATATTGGCAATGTCGGCACTAATGACGCCGATGAAGAATCTTCTCAGGTTCATGCCCAAGGTCTGGt < 1:1621229/68‑1 (MQ=255) cATATTGGCAATGTCGGCACTAATGACGCCGATGAAGAATCTTCTCAGGTTCATGCCCAAGGTCTGGt < 1:88791/68‑1 (MQ=255) cATATTGGCAATGTCGGCACTAATGACGCCGATGAAGAATCTTCTCAGGTTCATGCCCAAGGTCTGGt < 1:264938/68‑1 (MQ=255) cATATTGGCAATGTCGGCACTAATGACGCCGATGAAGAATCTTCTCAGGTTCATGCCCAAGGTCTGGt < 1:2937648/68‑1 (MQ=255) cATATTGGCAATGTCGGCACTAATGACGCCGATGAAGAATCTTCTCAGGTTCATGCCCAAGGTCTGGt < 1:2607729/68‑1 (MQ=255) cATATTGGCAATGTCGGCACTAATGACGCCGATGAAGAATCTTCTCAGGTTCATGCCCAAGGTCTGGt < 1:245105/68‑1 (MQ=255) cATATTGGCAATGTCGGCACTAATGACGCCGATGAAGAATCTTCTCAGGTTCATGCCCAAGGTCTGGt < 1:1212293/68‑1 (MQ=255) cATATTGGCAATGTCGGCACTAATGACGCCGATGAAGAATCTTCTCAGGTTCATGCCCAAGGTCTGGt < 1:2090354/68‑1 (MQ=255) cATATTGGCAATGTCGGCACTAATGACGCCGATGAAGAATCTTCTCAGGTTCATGCCCAAGGTCTGGt < 1:2061845/68‑1 (MQ=255) cATATTGGCAATGTCGGCACTAATGACGCCGATGAAGAATCTTCTCAGGTTCATGCCCAAGGTCTGGt < 1:413961/68‑1 (MQ=255) cATATTGGAAATGTCGGCACTAATGACGCCGATGAAGAATCTTCTCAGGTTCATGCCCAAGGTCTGGt < 1:2629200/68‑1 (MQ=255) ttGGCAATGTCGGCACCAATGACGCCGATGAAGAATCTTCTCAGGTACATGCACAAGGTCTGGTGAtta > 1:1291641/1‑68 (MQ=255) ttGGCAATGTCGGCACCAATGACGCCGATGAAGAATCTTCTCAGGTACATGCACAAGGTCTGGTGAt > 1:1534009/1‑67 (MQ=255) ttGGCAATGTCGGCACCAATGACGCCGATGAAGAATCTTCTCAGGTACATGCACAAGGTCTGGTGATTc > 1:2681245/1‑69 (MQ=255) ttGGCAATGTCGGCACCAATGACGCCGATGAAGAATCTTCTCAGGTACATGCACAAGGTCTGGTGATTc > 1:2881136/1‑69 (MQ=255) ttGGCAATGTCGGCACCAATGACGCCGATGAAGAATCTTCTCAGGTACATGCACAAGGTCTGGTGATTc > 1:1438525/1‑69 (MQ=255) ttGGCAATGTCGGCACCAATGACGCCGATGAAGAATCTTCTCAGGTACATGCACAAGGTCTGGTGATTc > 1:889666/1‑69 (MQ=255) ttGGCAATGTCGGCACCAATGACGCCGATGAAGAATCTTCTCAGGTACATGCACAAGGTCTGGTGATTc > 1:1000881/1‑69 (MQ=255) ttGGCAATGTCGGCACCAATGACGCCGATGAACAATCTTCTCAGGTACATGCACAAGGTCTTGTGATTc > 1:662870/1‑69 (MQ=255) ggCATTGTCGGCACTAATGACGCCGATGAAGAATCTTCACAGGTTCATGCCCAAGGTCTGGt < 1:782233/62‑1 (MQ=255) cAATGTCGGCACCAATGACGCCGATGAAGAATCTTCTCAGGTACATGCACAAGGTCTGGTGATTcgcg < 1:424802/68‑1 (MQ=255) cAATGTCGGCACCAATGACGCCGATGAAGAATCTTCTCAGGTACATGCACAAGGTCTGGTGATTcgcg < 1:1190458/68‑1 (MQ=255) cAATGTCGGCACCAATGACGCCGATGAAGAATCTTCTCAGGTACATGCACAAGGTCTGGTGATTcgcg < 1:198714/68‑1 (MQ=255) tCGGCACCAATGACGCCGATGAAGAATCTTCTCAGGTACATGcac > 1:2333408/1‑45 (MQ=255) tCGGCACCAATGACGCCGATGAAGAATCTTCTCAGGTACATGcac > 1:480373/1‑45 (MQ=255) tCGGCACCAATGACGCCGATGAAGAATCTTCTCAGGTACATGcac > 1:3112588/1‑45 (MQ=255) tCGGCACCAATGACGCCGATGAAGAATCTTCTCAGGTACATGcac > 1:740235/1‑45 (MQ=255) tCGGCACCAATGACGCCGATGAAGAATCTTCTCAGGTACATGcac > 1:1323986/1‑45 (MQ=255) tCGGCACCAATGACGCCGATGAAGAATCTTCTCAGGTACATGCACAAGGTCTGGTGATTCGCGACCTGc > 1:1883140/1‑69 (MQ=255) tCGGCACCAATGACGCCGATGAAGAATCTTCTCAGGTACATGCACAAGGTCTGGTGATTCGCGACCTGc > 1:2104704/1‑69 (MQ=255) tCGGCACCAATGACGCCGATGAAGAATCTTCTCAGGTACATGCACAAGGTCTGGTGATTCGCGACCTGc > 1:788851/1‑69 (MQ=255) tCGGCACCAATGACGCCGATGAAGAATCTTCTCAGGTACATGCACAAGGTCTGGTGATTCGCGACCTGc > 1:1237738/1‑69 (MQ=255) tCGGCACCAATGACGCCGATGAAGAATCTTCTCAGGTACATGCACAAGGTCTGGTGATTCGCGACCTGc > 1:1367647/1‑69 (MQ=255) tCGGCACCAATGACGCCGATGAAGAATCTTCTCAGGTACATGCACAAGGTCTGGTGATTCGCGACCTGc > 1:2927853/1‑69 (MQ=255) tCGGCACCAATGACGCCGATGAAGAATCTTCTCAGGTACATGCACAAGGTCTGGTGATTCGCGACCTGc > 1:1566534/1‑69 (MQ=255) tCGGCACCAAAGACGCCGATGAAGAATCTTCTCAGGTACATGcac > 1:1850671/1‑45 (MQ=255) aaTGACGCCGATGAAGAATCTTCTCAGGTACATGCACAAGGTCTGGTGATTCGCGACCTGCCGCTGAtt > 1:406178/1‑69 (MQ=255) aaTGACGCCGATGAAGAATCTTCTCAGGTACATGCACAAGGTCTGGTGATTCGCGACCTGCCGCTGAtt > 1:3164024/1‑69 (MQ=255) aaTGACGCCGATGAAGAATCTTCTCAGGTACATGCACAAGGTCTGGTGATTCGCGACCTGCCGCTGAtt > 1:1083177/1‑69 (MQ=255) aaTGACGCCGATGAAGAATCTTCTCAGGTACATGCACAAGGTCTGGTGATTCGCGACCTGCCGCTGAtt > 1:1509567/1‑69 (MQ=255) gaagaaTCTTCTCAGGTACATGCACAAGGTCTGGTGATTcgc < 1:1793335/42‑1 (MQ=255) gaagaaTCTTCTCAGGTACATGCACAAGGTCTGGTGATTcgc < 1:199105/42‑1 (MQ=255) gaagaaTCTTCTCAGGTACATGCACAAGGTCTGGTGATTcgc < 1:3247754/42‑1 (MQ=255) gaagaaTCTTCTCAGGTACATGCACAAGGTCTGGTGATTcgc < 1:2713489/42‑1 (MQ=255) gaagaaTCTTCTCAGGTACATGCACAAGGTCTGGTGATTcgc < 1:2256993/42‑1 (MQ=255) gaagaaTCTTCTCAGGTACATGCACAAGGTCTGGTGATTcgc < 1:2291299/42‑1 (MQ=255) gaagaaTCTTCTCAGGTACATGCACAAGGTCTGGTGATTcgc < 1:2999797/42‑1 (MQ=255) agaaTCTTCTCAGGTTCATGCCCAAGGTCTGGTGATTCGCGACCTGCCGCTGATTGCCAGCAACTTcc < 1:671613/68‑1 (MQ=255) agaaTCTTCTCAGGTTCATGCCCAAGGTCTGGTGATTCGCGACCTGCCGCTGATTGCCAGCAACTTcc < 1:3293338/68‑1 (MQ=255) agaaTCTTCTCAGGTTCATGCCCAAGGTCTGGTGATTCGCGACCTGCCGCTGATTGCCAGCAACTTcc < 1:660370/68‑1 (MQ=255) agaaTCTTCTCAGGTTCATGCCCAAGGTCTGGTGATTCGCGACCTGCCGCTGATTGCCAGCAACTTcc < 1:1703999/68‑1 (MQ=255) agaaTCTTCTCAGGTTCATGCCCAAGGTCTGGTGATTCGCGACCTGCCGCTGATTGCCAGCAACTTcc < 1:1691830/68‑1 (MQ=255) gaaTCTTCTCAGGTACATGCACAAGGTCTGGTGATTCGCGACCTGCCGCTGATTGCCAGCAACTTCCGt < 1:2845930/69‑1 (MQ=255) gaaTCTTCTCAGGTACATGCACAAGGTCTGGTGATTCGCGACCTGCCGCTGATTGCCAGCAACTTCCGt < 1:971288/69‑1 (MQ=255) gaaTCTTCTCAGGTACATGCACAAGGTCTGGTGATTCGCGACCTGCCGCTGATTGCCAGCAACTTCCGt < 1:2679654/69‑1 (MQ=255) gaaTCTTCTCAGGTACATGCACAAGGTCTGGTGATTCGCGACCTGCCGCTGATTGCCAGCAACTTCCGt < 1:3195027/69‑1 (MQ=255) gaaTCTTCTCAGGTACATGCACAAGGTCTGGTGATTCGCGACCTGCCGCTGATTGCCAGCAACTTCCGt < 1:850958/69‑1 (MQ=255) gaaTCTTCTCAGGTACATGCACAAGGTCTGGTGATTCGCGACCTGCCGCTGATTGCCAGCAACTTCCGt < 1:2735713/69‑1 (MQ=255) gaaTCTTCTCAGGTACATGCACAAGGTCTGGTGATTCGCGACCTGCCGCTGATTGCCAGCAACTTCCGt < 1:524675/69‑1 (MQ=255) gaaTCTTCTCAGGTACATGCACAAGGTCTGGTGATTCGCGACCTGCCGCTGATTGCCAGCAACTTCCGt < 1:536920/69‑1 (MQ=255) gaaTCTTCTCAGGTACATGCACAAGGTCTGGTGATTCGCGACCTGCCGCTGATTGCCAGCAACTTCCGt < 1:2917671/69‑1 (MQ=255) gaaTCTTCTCAGGTACATGCACAAGGTCTGGTGATTCGCGACCTGCCGCTGATTGCCAGCAACTTCCGt < 1:1804755/69‑1 (MQ=255) gaaTCTTCTCAGGTACATGCACAAGGTCTGGTGATTCGCGACCTGCCGCTGATTGACAGCAACTTCCGt < 1:513675/69‑1 (MQ=255) aaTCTTCTCAGGTACATGCACAAGGTCTGGTGATTCGCGACCTGCCGCTGATTGCCAGCAACTTCCGt < 1:1373749/68‑1 (MQ=255) aaTCTTCTCAGGTACATGCACAAGGTCTGGTGATTCGCGACCTGCCGCTGATTGCCAGCAACTTCCGt < 1:751258/68‑1 (MQ=255) tcttctCAGGTACATGCACAAGGTCTGGTGATTCGCGACCTGCCGCTGATTGCCAGCAACTTCCGTAAt > 1:85359/1‑69 (MQ=255) tcttctCAGGTACATGCACAAGGTCTGGTGATTCGCGACCTGCCGCTGATTGCCAGCAACTTCCGTAAt > 1:310183/1‑69 (MQ=255) ttctCAGGTACATGCACAAGGTCTGGTGATTCGCGACCTGCCGCTGATTGCCAGCAACTTCCGTAATa > 1:2731752/1‑68 (MQ=255) cAGGTACATGCACAAGGTCTGGTGATTCGCGACCTGCCGCTGATTGCCAGCAACTTCCGTAATACCg < 1:2625123/67‑1 (MQ=255) aGGTTCATGCCCAAGGTCTGGTGATTCGCGACCTGCCGCTGATTGCCAGCAACTTCCGTAATACCGAAg > 1:1019236/1‑69 (MQ=255) aGGTTCATGCCCAAGGTCTGGTGATTCGCGACCTGCCGCTGATTGCCAGCAACTTCCGTAATACCGAAg > 1:3050468/1‑69 (MQ=255) aGGTTCATGCCCAAGGTCTGGTGATTCGCGACCTGCCGCTGATTGCCAGCAACTTCCGTAATACCGAAg > 1:164051/1‑69 (MQ=255) aGGTTCATGCCCAAGGTCTGGTGATTCGCGACCTGCCGCTGATTGCCAGCAACTTCCGTAATACCGAAg > 1:2049620/1‑69 (MQ=255) aGGTTCATGACCAAGGTCTGGTGATTCGCGACCTGc > 1:966532/1‑36 (MQ=25) aGGTTCATGACCAAGGTCTGGTGATTCGCGACCTGCCGCTGATTGCCAGCAACTTCCGTAATACCGAAg > 1:3381901/1‑69 (MQ=255) aGGTTCATGACCAAGGTCTGGTGATTCGCGACCTGCCGCTGATTGCCAGCAACTTCCGTAATACCGAAg > 1:3161882/1‑69 (MQ=255) aGGTTCATGACCAAGGTCTGGTGATTCGCGACCTGCCGCTGATTGCCAGCAACTTCCGTAATACCGAAg > 1:2990667/1‑69 (MQ=255) aGGTTCATGACCAAGGTCTGGTGATTCGCGACCTGCCGCTGATTGCCAGCAACTTCCGTAATACCGAAg > 1:937106/1‑69 (MQ=255) aGGTTCATGACCAAGGTCTGGTGATTCGCGACCTGCCGCTGATTGCCAGCAACTTCCGTAATACCGAAg > 1:272553/1‑69 (MQ=255) aGGTTCATGACCAAGGTCTGGTGATTCGCGACCTGCCGCTGATTGCCAGCAACTTCCGTAATACCGAAg > 1:2599810/1‑69 (MQ=255) aGGTTCATGACCAAGGTCTGGTGATTCGCGACCTGCCGCTGATTGCCAGCAACTTCCGTAATACCGAAg > 1:3422321/1‑69 (MQ=255) aGGTTCATGACCAAGATCTGGTGATTCGCGACCTGCCGCTGATTGCCAGCAACTTCCGTAATACCGa > 1:2323676/1‑67 (MQ=255) aCATGCACAAGGTCTGGTGATTCGCGACCTGCCGCTGATTGCCAGCAACTTCCGTAATACCGAAGAcc > 1:813066/1‑68 (MQ=255) aCATGCACAAGGTCTGGTGATTCGCGACCTGCCGCTGATTGCCAGCAACTTCCGTAATACCGAAGAcc > 1:2738685/1‑68 (MQ=255) aCATGCACAAGGTCTGGTGATTCGCGACCTGCCGCTGATTGCCAGCAACTTCCGTAATACCGAAGAc > 1:2456634/1‑67 (MQ=255) aCATGCACAAGGTCTGGTGATTCGCGACCTGCCGCTGATTGCCAGCAACTTCCGTAATACCGAAGACct > 1:2364396/1‑69 (MQ=255) aCATGCACAAGGTCTGGTGATTCGCGACCTGCCGCTGATTGCCAGCAACTTCCGTAATACCGAAGACct > 1:610367/1‑69 (MQ=255) aCATGCACAAGGTCTGGTGATTCGCGACCTGCCGCTGATTGCCAGCAACTTCCGTAATACCGAAGACct > 1:1853110/1‑69 (MQ=255) aCATGCACAAGGTCTGGTGATTCGCGACCTGCCGCTGATTGCCAGCAACTTCCGTAATACCGAAGACct > 1:2932274/1‑69 (MQ=255) aCATGCACAAGGTCTGGTGATTCGCGACCTGCCGCTGATTGCCAGCAACTTCCGTAATACCGAAGACct > 1:298433/1‑69 (MQ=255) cacaAGGTCTGGTGATTCGCGACCTGCCGCTGATTGCCAGCAACTTCCGTAATACCGaa < 1:263902/59‑1 (MQ=255) cacaAGGTCTGGTGATTCGCGACCTGCCGCTGATTGCCAGCAACTTCCGTAATACCGaa < 1:1872894/59‑1 (MQ=255) cacaAGGTCTGGTGATTCGCGACCTGCCGCTGATTGCCAGCAACTTCCGTAATACCGaa < 1:1174215/59‑1 (MQ=255) cacaAGGTCTGGTGATTCGCGACCTGCCGCTGATTGCCAGCAACTTCCGTAATACCGaa < 1:922883/59‑1 (MQ=255) cacaAGGTCTGGTGATTCGCGACCTGCCGCTGATTGCCAGCAACTTCCGTAATACCGaa < 1:3140959/59‑1 (MQ=255) cacaAGGTCTGGTGATTCGCGACCTGCCGCTGATTGCCAGCAACTTCCGTAATACCGaa < 1:1081495/59‑1 (MQ=255) | ACTTATCCCCATATTGGCAATGTCGGCACCAATGACGCCGATGAAGAATCTTCTCAGGTACATGCACAAGGTCTGGTGATTCGCGACCTGCCGCTGATTGCCAGCAACTTCCGTAATACCGAAGACCT > minE/27494‑27621 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |