| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,431,447 | G→C | 49.3% | A250A (GCC→GCG) | rho ← | transcription termination factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,431,447 | 0 | G | C | 49.3% | ‑1.5 / 88.5 | 71 | A250A (GCC→GCG) | rho | transcription termination factor |
| Reads supporting (aligned to +/- strand): ref base G (26/10); new base C (13/22); total (39/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.20e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-04 | |||||||||||
CGCGCGCCAGACGAGTGATGGAGTCGAGCAGAATGATAACGTCTTTCTTGTGCTCAACCAGGCGTTTGGCCTTCTCGATCACCATTTCCGCAACCTGAACGTGGCGAGATGCGGGTTCGTCAAAGGTAGAAGCA > minE/2431380‑2431513 | cgcgcgcCAGACGAGTGATGGAGTCGAGCAGAATGATAACGTCTTTCTTGTGCTCAACCAGGCGTTTg < 1:2285803/68‑1 (MQ=255)cgcgcgcCAGACGAGTGATGGAGTCGAGCAGAATGATAACGTCTTTCTTGTGCTCAACCAGGCGTTTg < 1:819680/68‑1 (MQ=255)cgcgcgcCAGACGAGTGATGGAGTCGAGCAGAATGATAACGTCTTTCTTGTGCTCAACCAGGCGTTTg < 1:111217/68‑1 (MQ=255)cgcgcgcCAGACGAGTGATGGAGTCGAGCAGAATGATAACGTCTTTCTTGTGCTCAACCAGGCGTTTg < 1:2764129/68‑1 (MQ=255)cgcgcgcCAGACGAGTGATGGAGTCGAGCAGAATGATAACGTCTTTCTTGTGCTCAACCAGGCGTTTg < 1:1946368/68‑1 (MQ=255)cgcgcgcCAGACGAGTGATGGAGTCGAGCAGAATGATAACGTCTTTCTTGTGCTCAACCAGGCGTTTg < 1:3281599/68‑1 (MQ=255)cgcgcgcCAGACGAGTGATGGAGTCGAGCAGAATGATAACGTCTTTCTTGTGCTCAACCAGGCGTTTg < 1:161956/68‑1 (MQ=255)cgcgcgcCAGACGAGTGATGGAGTCGAGCAGAATGAAAACGTCTTTCTTGTGCTCAACCAGGCGTTTg < 1:898733/68‑1 (MQ=255) gcgcgcCAGACGAGTGATGGAGTCGAGCAGAATGATAACGTCTTTCTTGTGCTCAACCAGGCGTTTGGc < 1:403137/69‑1 (MQ=255) cgcgcCAGACGAGTGATGGAGTCGAGCAGAATGATAACGTCTTTCTTGTGCTCAACCAGGCGTTTCGcc < 1:983704/69‑1 (MQ=255) cgcgcCAGACGAGTGATGGAGTCGAGCAGAATGATAACGTCTTTCTTGTGCTCAACCAGGCGTTTCGcc < 1:2468170/69‑1 (MQ=255) cgcgcCAGACGAGTGATGGAGTCGAGCAGAATGATAACGTCTTTCTTGTGCTCAACCAGGCGTTTCGcc < 1:2350951/69‑1 (MQ=255) cgcgcCAGACGAGTGATGGAGTCGAGCAGAATGATAACGTCTTTCTTGTGCTCAACCAGGCGTTTCGcc < 1:2702986/69‑1 (MQ=255) cgcgcCAGACGAGTGATGGAGTCGAGCAGAATGATAACGTCTTTCTTGTGCTCAACCAGGCGTTTCGcc < 1:2734469/69‑1 (MQ=255) cgcgcCAGACGAGTGATGGAGTCGAGCAGAATGATAACGTCTTTCTTGTGCTCAACCAGGCGTTTCGcc < 1:3012656/69‑1 (MQ=255) cgcgcCAGACGAGTGATGGAGTCGAGCAGAATGATAACGTCTTTCTTGTGCTCAACCAGGCGTTTCGcc < 1:84072/69‑1 (MQ=255) cgcgcCAGACGAGTGATGGAGTCGAGCAGAATGATAACGTCTTTCTTGTGCTCAACCAGGCGTTTCGcc < 1:560695/69‑1 (MQ=255) gcgcCAGACGAGTGATGGAGTCGAGCAGAATGATAACGTCTTTCTTGTGCTCAACCAGGCGTTTCGcc < 1:53454/68‑1 (MQ=255) gcgcCAGACGAGTGATGGAGTCGAGCAGAATGATAACGTCTTTCTTGTGCTCAACCAGGCGTTTCGcc < 1:640872/68‑1 (MQ=255) gcgcCAGACGAGTGATGGAGTCGAGCAGAATGATAACGTCTTTCTTGTGCTCAACCAGGCGTTTCGcc < 1:2119389/68‑1 (MQ=255) gcgcCAGACGAGTGATGGAGTCGAGCAGAATGATAACGTCTTTCTTGTGCTCAACCAGGCGTTTCGcc < 1:3418831/68‑1 (MQ=255) gcgcCAGACGAGTGATGGAGTCGAGCAGAATGATAACGTCTTTCTTGTGCTCAACCAGGCGTTTCGcc < 1:1768078/68‑1 (MQ=255) gcgcCAGACGAGTGATGGAGTCGAGCAGAATGATAACGTCTTTCTTGTGCTCAACCAGGCGTTTCGcc < 1:1634499/68‑1 (MQ=255) cgcCAGACGAGTGATGGAGTCGAGCAGAATGATAACGTCTTTCTTGTGCTCAACCAGGCGTTTCGcc < 1:1281360/67‑1 (MQ=255) aGACGAGTGATGGAGTCGAGCAGAATGATAACGTCTTTCTTGTGCTCAACCAGGCGTTTGGCCTTCTCg < 1:354956/69‑1 (MQ=255) tGATGGAGTCGAGCAGAATGATAACGTCTTTCTTGTGCTCAACCAGGCGTTTg < 1:1972342/53‑1 (MQ=255) ggAGTCGAGCAGAATGATAACGTCTTTCTTGTGCTCAACCAGGCGTTTCGCCTTCTCGATCACCAttt < 1:1501961/68‑1 (MQ=255) ggAGTCGAGCAGAATGATAACGTCTTTCTTGTGCTCAACCAGGCGTTTCGCCTTCTCGATCACCAttt < 1:510919/68‑1 (MQ=255) ggAGTCGAGCAGAATGATAACGTCTTTCTTGTGCTCAACCAGGCGTTTCGCCTTCTCGATCACCAttt < 1:1916951/68‑1 (MQ=255) ggAGTCGAGCAGAATGATAACGTCTTTCTTGTGCTCAACCAGGCGTTTCGCCTTCTCGATCACCAttt < 1:1940405/68‑1 (MQ=255) ggAGTCGAGCAGAATGATAACGTCTTTCTTGTGCTCAACCAGGCGTTTCGCCTTCTCGATCACCAttt < 1:841179/68‑1 (MQ=255) ggAGTCGAGCAGAATGATAACGTCTTTCTTGTGCTCAACCAGGCGTTTCGCCTTCTCGATCACCAttt < 1:2043450/68‑1 (MQ=255) gAGTCGAGCAGAATGATAACGTCTTTCTTGTGCTCAACCAGGCGTTTCGCCTTCTCGATCACCAttt < 1:505362/67‑1 (MQ=255) gCAGAATGATAACGTCTTTCTTGTGCTCAACCAGGCGTTTGGCCTTCTCGATCACCATTTCCGCa < 1:98820/65‑1 (MQ=255) gCAGAATGATAACGTCTTTCTTGTGCTCAACCAGGCGTTTGGCCTTCTCGATCACCATTTCCGCa < 1:2661326/65‑1 (MQ=255) gCAGAATGATAACGTCTTTCTTGTGCTCAACCAGGCGTTTGGCCTTCTCGATCACCATTTCCGCa < 1:2799510/65‑1 (MQ=255) gCAGAATGATAACGTCTTTCTTGTGCTCAACCAGGCGTTTGGCCTTCTCGATCACCATTTCCGCa < 1:2825073/65‑1 (MQ=255) gCAGAATGATAACGTCTTTCTTGTGCTCAACCAGGCGTTTGGCCTTCTCGATCACCATTTCCGCa < 1:3293255/65‑1 (MQ=255) gCAGAATGATAACGTCTTTCTTGTGCTCAACCAGGCGTTTGGCCTTCTCGATCACCATTTCCGCa < 1:2151712/65‑1 (MQ=255) tGATAACGTCTTTCTTGTGCTCAACCAGGCGTTTGGCCt > 1:212695/1‑39 (MQ=255) tGATAACGTCTTTCTTGTGCTCAACCAGGCGTTTGGCCTtctc > 1:2951337/1‑43 (MQ=255) tGATAACGTCTTTCTTGTGCTCAACCAGGCGTTTGGCCTTCTCg > 1:1991852/1‑44 (MQ=255) tGATAACGTCTTTCTTGTGCTCAACCAGGCGTTTGGCCTTCTCg > 1:1825315/1‑44 (MQ=255) tGATAACGTCTTTCTTGTGCTCAACCAGGCGTTTGGCCTTCTCg > 1:461701/1‑44 (MQ=255) tGATAACGTCTTTCTTGTGCTCAACCAGGCGTTTGGCCTTCTCg > 1:1490704/1‑44 (MQ=255) tGATAACGTCTTTCTTGTGCTCAACCAGGCGTTTGGCCTTCTCg > 1:2736259/1‑44 (MQ=255) tGATAACGTCTTTCTTGTGCTCAACCAGGCGTTTGGCCTTCTCg > 1:2732191/1‑44 (MQ=255) tGATAACGTCTTTCTTGTGCTCAACCAGGCGTTTGGCCTTCTCg > 1:2188951/1‑44 (MQ=255) tGATAACGTCTTTCTTGTGCTCAACCAGGCGTTTGGCCTTCTCg > 1:1097089/1‑44 (MQ=255) tGATAACGTCTTTCTTGTGCTCAACCAGGCGTTTGGCCTTCTCGATCACCATTTCCGCa > 1:80142/1‑59 (MQ=255) tGATAACGTCTTTCTTGTGCTCAACCAGGCGTTTGGCCTTCTCGATCACCATTTCCGCa > 1:776902/1‑59 (MQ=255) tGATAACGTCTTTCTTGTGCTCAACCAGGCGTTTGGCCTTCTCGATCACCATTTCCGCa > 1:1224893/1‑59 (MQ=255) tGATAACGTCTTTCTTGTGCTCAACCAGGCGTTTGGCCTTCTCGATCACCATTTCCGCAACCTGAACgg < 1:1081593/69‑2 (MQ=255) tGATAACGTCTTTCTTGTGCTCAACCAGGCGTTTGGCCTTCTCGATCACCATTTCCGCAACCTGAACGt < 1:1502553/69‑1 (MQ=255) tctttcttGTGCTCAACCAGGCGTTTGGCCTTCTCGATCACCATTTCCGCAACCTGAACGTGGCGAGAt > 1:1090296/1‑69 (MQ=255) tttcttGTGCTCAACCAGGCGTTTGGCCTTCTCGATCACCATTTCCGCAACCTGAACGTGGCGAGATGc > 1:1827653/1‑69 (MQ=255) tttcttGTGCTCAACCAGGCGTTTGGCCTTCTCGATCACCATTTCCGCAACCTGAACGTGGCGAGATGc > 1:1339923/1‑69 (MQ=255) ttGTGCTCAACCAGGCGTTTCGCCTTCTCGATCACCATTTc > 1:1630143/1‑41 (MQ=255) ttGTGCTCAACCAGGCGTTTCGCCTTCTCGATCACCATTTCCg > 1:2880897/1‑43 (MQ=255) ttGTGCTCAACCAGGCGTTTCGCCTTCTCGATCACCATTTCCGCa > 1:3296389/1‑45 (MQ=255) ttGTGCTCAACCAGGCGTTTCGCCTTCTCGATCACCATTTCCGCa > 1:1809698/1‑45 (MQ=255) ttGTGCTCAACCAGGCGTTTCGCCTTCTCGATCACCATTTCCGCa > 1:2593440/1‑45 (MQ=255) ttGTGCTCAACCAGGCGTTTCGCCTTCTCGATCACCATTTCCGCa > 1:393965/1‑45 (MQ=255) ttGTGCTCAACCAGGCGTTTCGCCTTCTCGATCACCATTTCCGCa > 1:419428/1‑45 (MQ=255) ttGTGCTCAACCAGGCGTTTCGCCTTCTCGATCACCATTTCCGCa > 1:1951261/1‑45 (MQ=255) ttGTGCTCAACCAGGCGTTTCGCCTTCTCGATCACCATTTCCGCa > 1:1162314/1‑45 (MQ=255) ttGTGCTCAACCAGGCGTTTCGCCTTCTCGATCACCATTTCCGCa > 1:664222/1‑45 (MQ=255) ttGTGCTCAACCAGGCGTTTCGCCTTCTCGATCACCATTTCCGCa > 1:1036849/1‑45 (MQ=255) ttGTGCTCAACCAGGCGTTTCGCCTTCTCGATCACCATTTCCGCa > 1:2770737/1‑45 (MQ=255) ttGTGCTCAACCAGGCGTTTCGCCTTCTCGATCACCATTTCCGCa > 1:2309911/1‑45 (MQ=255) tGGCCTTCTCGATCACCATTTCCGCAACCTGAACGTGGCGAGATGCGGGTTCGTCAAAGGTAGAAGca > 1:1753035/1‑68 (MQ=255) tGGCCTTCTCGATCACCATTTCCGCAACCTGAACGTGGCGAGATGCGGGTTCGTCAAAGGTAGAAGca > 1:896062/1‑68 (MQ=255) tGGCCTTCTCGATCACCATTTCCGCAACCTGAACGTGGCGAGATGCGGGTTCGTCAAAGGTAGAAGca > 1:919439/1‑68 (MQ=255) tGGCCTTCTCGATCACCATTTCCGCAACCTGAACGTGGCGAGATGCGGGTTCGTCAAAGGTAGAAGca > 1:3196072/1‑68 (MQ=255) tGGCCTTCTCGATCACCATTTCCGCAACCTGAACGTGGCGAGATGCGGGTTCGTCAAAGGTAGAAGca > 1:3105279/1‑68 (MQ=255) tGGCCTTCTCGATCACCATTTCCGCAACCTGAACGTGGCGAGATGCGGGTTCGTCAAAGGTAGAAGca > 1:3014489/1‑68 (MQ=255) tGGCCTTCTCGATCACCATTTCCGCAACCTGAACGTGGCGAGATGCGGGTTCGTCAAAGGTAGAAGca > 1:1910222/1‑68 (MQ=255) tGGCCTTCTCGATCACCATTTCCGCAACCTGAACGTGGCGAGATGCGGGTTCGTCAAAGGTAGAAGca > 1:2781633/1‑68 (MQ=255) tGGCCTTCTCGATAACCATTTCCGCAACCTGAACGTGGCGAGATGCGGGTTCGTCAAAGGTAGAAGca > 1:1092729/1‑68 (MQ=255) tGGACTTCTCGATCACCATTTCCGCAACCTGAACGTGGCGAGATGCGGGTTCGTCAAAGGTAGAAGca > 1:1028204/1‑68 (MQ=255) | CGCGCGCCAGACGAGTGATGGAGTCGAGCAGAATGATAACGTCTTTCTTGTGCTCAACCAGGCGTTTGGCCTTCTCGATCACCATTTCCGCAACCTGAACGTGGCGAGATGCGGGTTCGTCAAAGGTAGAAGCA > minE/2431380‑2431513 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |