| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,433,207 | T→C | 35.3% | P75P (CCT→CCC) | rhlB → | ATP‑dependent RNA helicase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,433,207 | 0 | T | C | 35.3% | 30.0 / 25.7 | 48 | P75P (CCT→CCC) | rhlB | ATP‑dependent RNA helicase |
| Reads supporting (aligned to +/- strand): ref base T (19/12); new base C (3/14); total (22/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.91e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.46e-01 | |||||||||||
GCAAACCGGTACCGGGAAAACGATGGCGTTTCTTACGTCAACGTTTCATTATCTTCTCTCTCATCCTGCGATTGCCGATCGCAAGGTGAATCAGCCGCGTGCCTTAATTATGGCACCGA > minE/2433141‑2433259 | gcAAACCGGTACCGGGAAAACGATGGCGTTTCTTACGTCAACGTTTCATTATCTTCTCTCTCATCCt > 1:622907/1‑67 (MQ=255)gcAAACCGGTACCGGGAAAACGATGGCGTTTCTTACGTCAACGTTTCATTATCTTCTCTCTCATCCt > 1:276339/1‑67 (MQ=255)gcAAACCGGTACCGGGAAAACGATGGCGTTTCTTACGTCAACGTTTCATTATCTTCTCTCTCATCCt > 1:1023716/1‑67 (MQ=255)gcAAACCGGTACCGGGAAAACGATGGCGTTTCTTACGTCAACGTTTCATTATCTTCTCTCTCATCCt > 1:2186343/1‑67 (MQ=255)gcAAACCGGTACCGGGAAAACGATGGCGTTTCTTACGTCAACGTTTCATTATCTTCTCTCTCATCCt > 1:1704563/1‑67 (MQ=255) cAAACCGGTACCGGGAAAACGATGGCGTTTCTTACGTCAACGTTTCATTATCTTCTCTCTCATCCTGCg < 1:344283/69‑1 (MQ=255) cAAACCGGTACCGGGAAAACGATGGCGTTTCTTACGTCAACGTTTCATTATCTTCTCTCTCATCCTGCg < 1:3276465/69‑1 (MQ=255) cAAACCGGTACCGGGAAAACGATGGCGTTTCTTACGTCAACGTTTCATTATCTTCTCTCTCATCCTGCg < 1:1433404/69‑1 (MQ=255) cAAACCGGTACCGGGAAAACGATGGCGTTTCTTACGTCAACGTTTCATTATCTTCTCTCTCATCCTGCg < 1:2979067/69‑1 (MQ=255) cAAACCGGTACCGGGAAAACGATGGCGTTTCTTACGTCAACGTTTCATTATCTTCTCTCTCATCCTGCg < 1:1757181/69‑1 (MQ=255) aCCGGTACCGGGAAAACGATGGCGTTTCTTACGTCAACGTTTCATTATCTTCTCTCTCATCCTGCGAtt > 1:475923/1‑69 (MQ=255) aCCGGTACCGGGAAAACGATGGCGTTTCTTACGTCAACGTTTCATTATCTTCTCTCTCATCCTGCGAtt > 1:3244702/1‑69 (MQ=255) aCCGGTACCGGGAAAACGATGGCGTTTCTTACGTCAACGTTTCATTATCTTCTCTCTCATCCTGCGAtt > 1:533477/1‑69 (MQ=255) aCCGGTACCGGGAAAACGATGGCGTTTCTTACGTCAACGTTTCATTATCTTCTCTCTCATCCTGCGAtt > 1:1448/1‑69 (MQ=255) ccGGTACCGGGAAAACGATGGCGTTTCTTACGTCAACGTTTCATTATCTTCTCTCTCATCCTGCGATTg < 1:2075361/69‑1 (MQ=255) ccGGTACCGGGAAAACGATGGCGTTTCTTACGTCAACGTTTCATTATCTTCTCTCTCATCCTGCGATTg < 1:1478068/69‑1 (MQ=255) gTACCGGGAAAACGATGGCGTTTCTTACGTCAACGTTTCATTATCTTCTCTCTCATCCCGCGATTGCCg < 1:1828401/69‑1 (MQ=255) gTACCGGGAAAACGATGGCGTTTCTTACGTCAACGTTTCATTATCTTCTCTCTCATCCCGCGATTGCCg < 1:1170726/69‑1 (MQ=255) gTACCGGGAAAACGATGGCGTTTCTTACGTCAACGTTTCATTATCTTCTCTCTCATCCCGCGATTGCCg < 1:3427634/69‑1 (MQ=255) gTACCGGGAAAACGATGGCGTTTCTTACGTCAACGTTTCATTATCTTCTCTCTCATCCCGCGATTGCCg < 1:1173624/69‑1 (MQ=255) gTACCGGGAAAACGATGGCGTTTCTTACGTCAACGTTTCATTATCTTCTCTCTCATCCCGCGATTGCCg < 1:3108427/69‑1 (MQ=255) gTACCGGGAAAACGATGGCGTTTCTTACGTCAACGTTTCATTATCTTCTCTCTCATCCCGCGATTGCCg < 1:2894939/69‑1 (MQ=255) gTACCGGGAAAACGATGGCGTTTCTTACGTCAACGTTTCATTATCTTCTCTCTCATCCCGCGATTGCCg < 1:2879094/69‑1 (MQ=255) gTACCGGGAAAACGATGGCGTTTCTTACGTCAACGTTTCATTATCTTCTCTCTCATCCCGCGATTGCCg < 1:2583562/69‑1 (MQ=255) gTACCGGGAAAACGATGGCGTTTCTTACGTCAACGTTTCATTATCTTCTCTCTCATCCCGCGATTGCCg < 1:2527327/69‑1 (MQ=255) gTACCGGGAAAACGATGGCGTTTCTTACGTCAACGTTTCATTATCTTCTCTCTCATCCCGCGATTGCCg < 1:68510/69‑1 (MQ=255) gTACCGGGAAAACGATGGCGTTTCTTACGTCAACGTTTCATTATCTTCTCTCTCATCCCGCGATTGCCg < 1:201827/69‑1 (MQ=255) gTACCGGGAAAACGATGGCGTTTCTTACGTCAACGTTTCATTATCTTCTCTCTCATCCCGCGATTGCCg < 1:2561370/69‑1 (MQ=255) gTACCGGGAAAACGATGGCGTTTCTTACGTCAACGTTTCATTATCTTCTCTCTCATCCCGCGATTGCCg < 1:218463/69‑1 (MQ=255) gggAAAACGATGGCGTTTCTTACGTCAACGTTTCATTATCTTCTCTCTCATCCCGCGATTGCCg < 1:1484278/64‑1 (MQ=255) aaaaCGATGGCGTTTCTTACGTCAACGTTTCATTATCTTCTCTCTCATCCTGCGATTGCCGATCGCAAg > 1:2073719/1‑69 (MQ=255) aTGGCGTTTCTTACGTCAACGTTTCATTATCTTCTCTCTCATCCTGCGATTGCCGATCGCAAGGTGa > 1:1224142/1‑67 (MQ=255) aTGGCGTTTCTTACGTCAACGTTTCATTATCTTCTCTCTCATCCTGCGATTGCCGATCGCAAGGTGa > 1:1621510/1‑67 (MQ=255) aTGGCGTTTCTTACGTCAACGTTTCATTATCTTCTCTCTCATCCTGCGATTGCCGATCGCAAGGTGa > 1:1466383/1‑67 (MQ=255) aTGGCGTTTCTTACGTCAACGTTTCATTATCTTCTCTCTCATCCTGCGATTGCCGATCGCAAGGTGa > 1:51639/1‑67 (MQ=255) aTGGCGTTTCTTACGTCAACGTTTCATTATCTTCTCTCTCATCCTGCGATTGCCGATCGCAAGGTGa > 1:1914318/1‑67 (MQ=255) cGTTTCTTACGTCAACGTTTCATTATCTTCTCTCTCATCCCGCGATTGCCg > 1:391872/1‑51 (MQ=255) cGTTTCTTACGTCAACGTTTCATTATCTTCTCTCTCATCCCGCGATTGCCGATCGCaa > 1:824684/1‑58 (MQ=255) cGTTTCTTACGTCAACGTTTCATTATCTTCTCTCTCATCCCGCGATTGCCGATCGCaa > 1:3311757/1‑58 (MQ=255) tttCTTACGTCAACGTTTCATTATCTTCTCTCTCATCCTGCGATTGCCGATCGCAAGGTGAATCAGcc > 1:667538/1‑68 (MQ=255) tCTTACGTCAACGTTTCATTATCTTCTCTCTCATCCTGCGATTGCCGATCGCaa > 1:2407695/1‑54 (MQ=255) tCTTACGTCAACGTTTCATTATCTTCTCTCTCATCCTGCGATTGCCGATCGCAAGGTGAATCAGCcgcg > 1:2685765/1‑69 (MQ=255) tCTTACGTCAACGTTTCATTATCTTCTCTCTCATCCTGCGATTGCCGATCGCAAGGTGAATCAGCcgcg > 1:2109760/1‑69 (MQ=255) tACGTCAACGTTTCATTATCTTCTCTCTCATCCTGCGATTGCCGATCGCAAGGTGAATCAGCCGCGTg < 1:3337112/68‑1 (MQ=255) tACGTCAACGTTTCATTATCTTCTCTCTCATCCTGCGATTGCCGATCGCAAGGTGAATCAGCCGCGTg < 1:168532/68‑1 (MQ=255) tACGTCAACGTTTCATTATCTTCTCTCTCATCCTGCGATTGCCGATCGCAAGGTGAATCAGCCGCGTg < 1:878589/68‑1 (MQ=255) aCGTCAACGTTTCATTATCTTCTCTCTCATCCTGCGATTGCCGATCGCAAGGTGAATCAGCCGCGTg < 1:306982/67‑1 (MQ=255) aCGTCAACGTTTCATTATCTTCTCTCTCATCCTGCGATTGCCGATCGCAAGGTGAATCAGCCGCGTg < 1:996370/67‑1 (MQ=255) aTCTTCTCTCTCCTCCTGCGATTGCCGCTCGCAAGGTGAATCAGCCGCGTGCCTTAATTATGGCAcc > 1:683857/1‑67 (MQ=255) aTCTTCTCTCTCATCCTGCGATTGCCGATCGCAAGGTGAATCAGCCGCGTGCCTTAATTATGGCACCGa > 1:551738/1‑69 (MQ=255) aTCTTCTCTCTCATCCTGCGATTGCCGATCGCAAGGTGAATCAGCCGCGTGCCTTAATTATGGCACCGa > 1:555711/1‑69 (MQ=255) aTCTTCTCTCTCATCCTGCGATTGCCGATCGCAAGGTGAATCAGCCGCGTGCCTTAATTATGGCACCGa > 1:2799869/1‑69 (MQ=255) aTCTTCTCTCTCATCCTGCGATTGCCGATCGCAAGGTGAATCAGCCGCGTGCCTTAATTATGGCACCGa > 1:1201827/1‑69 (MQ=255) | GCAAACCGGTACCGGGAAAACGATGGCGTTTCTTACGTCAACGTTTCATTATCTTCTCTCTCATCCTGCGATTGCCGATCGCAAGGTGAATCAGCCGCGTGCCTTAATTATGGCACCGA > minE/2433141‑2433259 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |