| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,434,324 | A→C | 39.9% | intergenic (+76/‑60) | rhlB → / → gpp | ATP‑dependent RNA helicase/guanosine pentaphosphatase/exopolyphosphatase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,434,324 | 0 | A | C | 39.9% | 49.1 / 114.9 | 118 | intergenic (+76/‑60) | rhlB/gpp | ATP‑dependent RNA helicase/guanosine pentaphosphatase/exopolyphosphatase |
| Reads supporting (aligned to +/- strand): ref base A (53/18); new base C (26/21); total (79/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.49e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.61e-01 | |||||||||||
Only 100 of 127 total aligned reads displayed.
TGTCATACTCAAAATCATTCAAACTGTATCAAGATGGCAAGAGAATGAATCCCGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAGATTGAAGGATGAAGAG > minE/2434257‑2434382 | tGTCATACTCAAAATCATTCAAACTGTATCAAGATGGCAAGAGGATGAATCCCGATGAGCTTACTgta > 1:1936751/1‑68 (MQ=255)tGTCATACTCAAAATCATTCAAACTGTATCAAGATGGCAAGAGAATGAATCCCGATGAGCTTACTgta > 1:1765137/1‑68 (MQ=255)tGTCATACTCAAAATCATTCAAACTGTATCAAGATGGCAAGAGAATGAATCCCGATGAGCTTACTgta > 1:1783619/1‑68 (MQ=255)tGTCATACTCAAAATCATTCAAACTGTATCAAGATGGCAAGAGAATGAATCCCGATGAGCTTACTgta > 1:2487621/1‑68 (MQ=255)tGTCATACTCAAAATCATTCAAACTGTATCAAGATGGCAAGAGAATGAATCCCGATGAGCTTACTgta > 1:1657245/1‑68 (MQ=255)tGTCATACTCAAAATCATTCAAACTGTATCAAGATGGCAAGAGAATGAATCCCGATGAGCTTACTgta > 1:666846/1‑68 (MQ=255) cTCAAAATCATTCAAACTGTATCAAGATGGCAAGAGAATGAATCCCGATGAGCTTACTGTAGTAAGt > 1:1912806/1‑67 (MQ=255) cTCAAAATCATTCAAACTGTATCAAGATGGCAAGAGAATGAATCCCGATGAGCTTACTGTAGTAAGt > 1:3250310/1‑67 (MQ=255) cTCAAAATCATTCAAACTGTATCAAGATGGCAAGAGAATGAATCCCGATGAGCTTACTGTAGTAAGt > 1:547809/1‑67 (MQ=255) cTCAAAATCATTCAAACTGTATCAAGATGGCAAGAGAATGAATCCCGATGAGCTTACTGTAGTAAGt > 1:1911652/1‑67 (MQ=255) cTCAAAATCATTCAAACTGTATCAAGATGGCAAGAGAATGAATCCCGATGAGCTTACTGTAGTAAGt > 1:759090/1‑67 (MQ=255) cTCAAAATCATTCAAACTGTATCAAGATGGCAAGAGAATGAATCCCGATGAGCTTACTGTAGTAAGt > 1:1143170/1‑67 (MQ=255) cTCAAAATCATTCAAACTGTATCAAGATGGCAAGAGAATGAATCCCGATGAGCTTACTGTAGTAAGt > 1:2393373/1‑67 (MQ=255) cTCAAAATCATTCAAACTGTATCAAGATGGCAAGAGAATGAATCCCGATGAGCTTACTGTAGTAAGt > 1:1066381/1‑67 (MQ=255) cTCAAAATCATTCAAACTGTATCAAGATGGCAAGAGAATGAATCCCGATGAGCTTACTGTAGTAAGTGa > 1:1852510/1‑69 (MQ=255) cTCAAAATCATTCAAACTGTATCAAGATGGCAAGAGAATGAATCCCGATGAGCTTACTGTAGTAAGTGa > 1:3284912/1‑69 (MQ=255) cTCAAAATCATTCAAACTGTATCAAGATGGCAAGAGAATGAATCCCGATGAGCTTACTGTAGTAAGTGa > 1:2288047/1‑69 (MQ=255) cTCAAAATCATTCAAACTGTATCAAGATGGCAAGAGAATGAATCCCGATGAGCTTACTGTAGTAAGTGa > 1:2095587/1‑69 (MQ=255) cTCAAAATCATTCAAACTGTATCAAGATGGCAAGAGAATGAATCCCGATGAGCTTACTGTAGTAAGTGa > 1:2874811/1‑69 (MQ=255) cTCAAAATCATTCAAACTGTATCAAGATGGCAAGAGAATGAATCCCGATGAGCTTACTGTAGTAAGTGa > 1:3044032/1‑69 (MQ=255) cTCAAAATCATTCAAACTGTATCAAGATGGCAAGAGAATGAATCCCGATGAGCTTACTGTAGTAAGTGa > 1:2534768/1‑69 (MQ=255) cTCAAAATCATTCAAACTGTATCAAGATGGCAAGAGAATGAATCCCGATGAGCTTACTGTAGTAAGTGa > 1:2599995/1‑69 (MQ=255) cTCAAAATCATTCAAACTGTATCAAGATGGCAAGAGAATGAATCCCGATGAGCTTACTGTAGTAAGTGa > 1:2783669/1‑69 (MQ=255) cTCAAAATCATTCAAACTGTATCAAGATGGCAAGAGAATGAATCCCGATGAGCTTACTGTAGTAAGTGa > 1:1012446/1‑69 (MQ=255) cTCAAAATCATTCAAACTGTATCAAGATGGCAAGAGAATGAATCCCGATGAGCTTACTGTAGTAAGTGa > 1:434786/1‑69 (MQ=255) cTCAAAATCATTCAAACTGTATCAAGATGGCAAGAGAATGAATCCCGATGAGCTTACTGTAGTAAGTGa > 1:1585439/1‑69 (MQ=255) cTCAAAATCATTCAAACTGTATCAAAATGGCAAGAGAATGAATCCCGATGAGCTTACTGTAGTAAGTGa > 1:2278486/1‑69 (MQ=255) aTTCAAACTGTATCAAGATGGCAAGAGAATGAATCCCGATGAGCTTACTGTAGTAAGTGATTCAgg > 1:572273/1‑66 (MQ=255) aTTCAAACTGTATCAAGATGGCAAGAGAATGAATCCCGATGAGCTTACTGTAGTAAGTGATTCAGGt > 1:191365/1‑67 (MQ=255) aTTCAAACTGTATCAAGATGGCAAGAGAATGAATCCCGATGAGCTTACTGTAGTAAGTGATTCAGGt > 1:3150665/1‑67 (MQ=255) aTTCAAACTGTATCAAGATGGCAAGAGAATGAATCCCGATGAGCTTACTGTAGTAAGTGATTCAGGt > 1:2591323/1‑67 (MQ=255) aCTGTATCAAGATGGCAAGAGAATGAATCCCGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGaa < 1:1105307/68‑1 (MQ=255) aCTGTATCAAGATGGCAAGAGAATGAATCCCGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGaa < 1:3011308/68‑1 (MQ=255) tCAAGATGGCAAGAGAATGAATCCCGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGcc > 1:880265/1‑69 (MQ=255) tCAAGATGGCAAGAGAATGAATCCCGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGc > 1:2491450/1‑68 (MQ=255) gCAAGAGAATGAATCCCGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACaga > 1:3369359/1‑69 (MQ=255) aaGAGAATGAATCCCGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAgaga < 1:1528210/69‑1 (MQ=255) aaGAGAATGAATCCCGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAgaga < 1:2037698/69‑1 (MQ=255) aaGAGAATGAATCCCGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAgaga < 1:936750/69‑1 (MQ=255) aaGAGAATGAATCCCGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAgaga < 1:226303/69‑1 (MQ=255) ataatgaatCCCGATGCGCTTACTGTCGTAAGTGATTCAGGTGAAGGAATGCAGCCAACGCAGAGACAg < 1:2793406/67‑1 (MQ=255) agaATGAATCCCGATGCGCTTACTGTCGTAAGTGATTCAGGTGAAGGAATGCAGCCAGCGCAGAGACAg < 1:2243906/69‑1 (MQ=38) agaATGAATCCCGATGCGCTTACTGTCGTAAGTGATTCAGGTGAAGGAATGCAGCCAACGCAGAGACAg < 1:247510/69‑1 (MQ=38) agaATGAATCCCGATGCGCTTACTGTCGTAAGTGATTCAGGTGAAGGAATGCAGCCAACGCAGAGACAg < 1:1737892/69‑1 (MQ=38) agaATGAATCCCGATGCGCTTACTGTCGTAAGTGATTCAGGTGAAGGAATGCAGCCAACGCAGAGACAg < 1:2970746/69‑1 (MQ=38) agaATGAATCCCGATGCGCTTACTGTCGTAAGTGATTCAGGTGAAGGAATGCAGCCAACGCAGAGACAg < 1:1681708/69‑1 (MQ=38) agaATGAATCCCGATGCGCTTACTGTCGTAAGTGATTCAGGTGAAGGAATGCAGCCAACGCAGAGACAg < 1:1818469/69‑1 (MQ=38) agaATGAATCCCGATGCGCTTACTGTCGTAAGTGATTCAGGTGAAGGAATGCAGCCAACGCAGAGACAg < 1:1889647/69‑1 (MQ=38) agaATGAATCCCGATGCGCTTACTGTCGTAAGTGATTCAGGTGAAGGAATGCAGCCAACGCAGAGACAg < 1:3406069/69‑1 (MQ=38) agaATGAATCCCGATGCGCTTACTGTCGTAAGTGATTCAGGTGAAGGAATGCAGCCAACGCAGAGACAg < 1:3231795/69‑1 (MQ=38) agaATGAATCCCGATGCGCTTACTGTCGTAAGTGATTCAGGTGAAGGAATGCAGCCAACGCAGAGACAg < 1:3214674/69‑1 (MQ=38) agaATGAATCCCGATGCGCTTACTGTCGTAAGTGATTCAGGTGAAGGAATGCAGCCAACGCAGAGACAg < 1:780215/69‑1 (MQ=38) agaATGAATCCCGATGCGCTTACTGTCGTAAGTGATTCAGGTGAAGGAATGCAGCCAACGCAGAGACAg < 1:238409/69‑1 (MQ=38) agaATGAATCCCGATGCGCTTACTGTCGTAAGTGATTCAGGTGAAGGAATGCAGCCAACGCAGAGACAg < 1:2882237/69‑1 (MQ=38) agaATGAATCCCGATGCGCTTACTGTCGTAAGTGATTCAGGTGAAGGAATGCAGCCAACGCAGAGACAg < 1:751995/69‑1 (MQ=38) agaATGAATCCCGATGCGCTTACTGTCGTAAGTGATTCAGGTGAAGGAATGCAGCCAACGCAGAGACAg < 1:645932/69‑1 (MQ=38) aatgaatCCCGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAgaga < 1:327278/64‑1 (MQ=255) tgaatCCCGATGCGCTTACTGTCGTAAGTGATTCTGGTGAAGGaa > 1:316690/1‑45 (MQ=255) tgaatCCCGATGCGCTTACTGTCGTAAGTGATTCAGGTGAAGGaa > 1:1064655/1‑45 (MQ=255) tgaatCCCGATGCGCTTACTGTCGTAAGTGATTCAGGTGAAGGaa > 1:2906564/1‑45 (MQ=255) tgaatCCCGATGCGCTTACTGTCGTAAGTGATTCAGGTGAAGGaa > 1:3010971/1‑45 (MQ=255) tgaatCCCGATGCGCTTACTGTCGTAAGTGATTCAGGTGAAGGaa > 1:3013196/1‑45 (MQ=255) tgaatCCCGATGCGCTTACTGTCGTAAGTGATTCAGGTGAAGGaa > 1:3362117/1‑45 (MQ=255) tgaatCCCGATGCGCTTACTGTCGTAAGTGATTCAGGTGAAGGaa > 1:2871977/1‑45 (MQ=255) tgaatCCCGATGCGCTTACTGTCGTAAGTGATTCAGGTGAAGGaa > 1:1615603/1‑45 (MQ=255) tgaatCCCGATGCGCTTACTGTCGTAAGTGATTCAGGTGAAGGaa > 1:1662541/1‑45 (MQ=255) tgaatCCCGATGCGCTTACTGTCGTAAGTGATTCAGGTGAAGGaa > 1:173364/1‑45 (MQ=255) tgaatCCCGATGCGCTTACTGTCGTAAGTGATTCAGGTGAAGGaa > 1:1962081/1‑45 (MQ=255) tgaatCCCGATGCGCTTACTGTCGTAAGTGATTCAGGTGAAGGaa > 1:1250758/1‑45 (MQ=255) tgaatCCCGATGCGCTTACTGTCGTAAGTGATTCAGGTGAAGGaa > 1:2121289/1‑45 (MQ=255) tgaatCCCGATGCGCTTACTGTCGTAAGTGATTCAGGTGAAGGaa > 1:2136759/1‑45 (MQ=255) tgaatCCCGATGCGCTTACTGTCGTAAGTGATTCAGGTGAAGGaa > 1:2208510/1‑45 (MQ=255) tgaatCCCGATGCGCTTACTGTCGTAAGTGATTCAGGTGAAGGaa > 1:1177818/1‑45 (MQ=255) tgaatCCCGATGCGCTTACTGTCGTAAGTGATTCAGGTGAAGGaa > 1:1171911/1‑45 (MQ=255) tgaatCCCGATGCGCTTACTGTCGTAAGTGATTCAGGTGAAGGaa > 1:1124526/1‑45 (MQ=255) tgaatCCCGATGCGCTTACTGTCGTAAGTGATTCAGGTGAAGGaa > 1:2658380/1‑45 (MQ=255) tgaatCCCGATGCGCTTACTGTCGTAAGTGATTCAGGTGAAGGaa > 1:2693560/1‑45 (MQ=255) tgaatCCCGATGCGCTTACTGTCGTAAGTGATTCAGGTGAAGGa > 1:523442/1‑44 (MQ=38) tCCCGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAGATTGAAg < 1:2356100/68‑1 (MQ=255) tCCCGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAGATTGAAg < 1:1818730/68‑1 (MQ=255) tCCCGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAGATTGAAg < 1:2844276/68‑1 (MQ=255) tCCCGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAGATTGAAg < 1:410314/68‑1 (MQ=255) ccGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACa > 1:1310273/1‑58 (MQ=255) ccGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACa > 1:1341376/1‑58 (MQ=255) ccGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACa > 1:949083/1‑58 (MQ=255) ccGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACa > 1:1353040/1‑58 (MQ=255) ccGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACa > 1:2323889/1‑58 (MQ=255) ccGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACa > 1:2403281/1‑58 (MQ=255) ccGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACa > 1:2651011/1‑58 (MQ=255) ccGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACa > 1:2849263/1‑58 (MQ=255) ccGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAGATTGAAGGa > 1:2046397/1‑68 (MQ=255) ccGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAGATTGAAGGa > 1:692307/1‑68 (MQ=255) aGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAGATTGAAGGATGAagag > 1:355976/1‑69 (MQ=255) aGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAGATTGAAGGATGAagag > 1:1797738/1‑69 (MQ=255) aGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAGATTGAAGGATGAagag < 1:581143/69‑1 (MQ=255) aGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAGATTGAAGGATGAagag < 1:755567/69‑1 (MQ=255) aGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAGATTGAAGGATGAagag > 1:1127036/1‑69 (MQ=255) aGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAGATTGAAGGATGAagag < 1:1075338/69‑1 (MQ=255) aGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGAAAGATTGAAGGATGAagag < 1:3367763/69‑1 (MQ=255) agtaAGTGATTGAGGTGTTAGATTGCTGCCTACACAGAGACAGTTTGAAg < 1:1178443/50‑1 (MQ=255) | TGTCATACTCAAAATCATTCAAACTGTATCAAGATGGCAAGAGAATGAATCCCGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAGATTGAAGGATGAAGAG > minE/2434257‑2434382 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |