| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,434,343 | A→G | 38.8% | intergenic (+95/‑41) | rhlB → / → gpp | ATP‑dependent RNA helicase/guanosine pentaphosphatase/exopolyphosphatase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,434,343 | 0 | A | G | 38.8% | 60.5 / 82.6 | 121 | intergenic (+95/‑41) | rhlB/gpp | ATP‑dependent RNA helicase/guanosine pentaphosphatase/exopolyphosphatase |
| Reads supporting (aligned to +/- strand): ref base A (45/29); new base G (26/21); total (71/50) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.75e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.66e-01 | |||||||||||
Only 100 of 127 total aligned reads displayed.
CAAACTGTATCAAGATGGCAAGAGAATGAATCCCGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAGATTGAAGGATGAAGAGTATGGGTTCCACCTCGTCGCTGTATGC > minE/2434276‑2434409 | aCTGTATCAAGATGGCAAGAGAATGAATCCCGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGaa < 1:1105307/68‑1 (MQ=255) aCTGTATCAAGATGGCAAGAGAATGAATCCCGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGaa < 1:3011308/68‑1 (MQ=255) tCAAGATGGCAAGAGAATGAATCCCGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGcc > 1:880265/1‑69 (MQ=255) tCAAGATGGCAAGAGAATGAATCCCGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGc > 1:2491450/1‑68 (MQ=255) gCAAGAGAATGAATCCCGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACaga > 1:3369359/1‑69 (MQ=255) aaGAGAATGAATCCCGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAgaga < 1:1528210/69‑1 (MQ=255) aaGAGAATGAATCCCGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAgaga < 1:226303/69‑1 (MQ=255) aaGAGAATGAATCCCGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAgaga < 1:2037698/69‑1 (MQ=255) aaGAGAATGAATCCCGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAgaga < 1:936750/69‑1 (MQ=255) ataatgaatCCCGATGCGCTTACTGTCGTAAGTGATTCAGGTGAAGGAATGCAGCCAACGCAGAGACAg < 1:2793406/67‑1 (MQ=255) agaATGAATCCCGATGCGCTTACTGTCGTAAGTGATTCAGGTGAAGGAATGCAGCCAGCGCAGAGACAg < 1:2243906/69‑1 (MQ=38) agaATGAATCCCGATGCGCTTACTGTCGTAAGTGATTCAGGTGAAGGAATGCAGCCAACGCAGAGACAg < 1:3231795/69‑1 (MQ=38) agaATGAATCCCGATGCGCTTACTGTCGTAAGTGATTCAGGTGAAGGAATGCAGCCAACGCAGAGACAg < 1:1737892/69‑1 (MQ=38) agaATGAATCCCGATGCGCTTACTGTCGTAAGTGATTCAGGTGAAGGAATGCAGCCAACGCAGAGACAg < 1:1681708/69‑1 (MQ=38) agaATGAATCCCGATGCGCTTACTGTCGTAAGTGATTCAGGTGAAGGAATGCAGCCAACGCAGAGACAg < 1:2970746/69‑1 (MQ=38) agaATGAATCCCGATGCGCTTACTGTCGTAAGTGATTCAGGTGAAGGAATGCAGCCAACGCAGAGACAg < 1:1889647/69‑1 (MQ=38) agaATGAATCCCGATGCGCTTACTGTCGTAAGTGATTCAGGTGAAGGAATGCAGCCAACGCAGAGACAg < 1:3214674/69‑1 (MQ=38) agaATGAATCCCGATGCGCTTACTGTCGTAAGTGATTCAGGTGAAGGAATGCAGCCAACGCAGAGACAg < 1:3406069/69‑1 (MQ=38) agaATGAATCCCGATGCGCTTACTGTCGTAAGTGATTCAGGTGAAGGAATGCAGCCAACGCAGAGACAg < 1:238409/69‑1 (MQ=38) agaATGAATCCCGATGCGCTTACTGTCGTAAGTGATTCAGGTGAAGGAATGCAGCCAACGCAGAGACAg < 1:725034/69‑1 (MQ=38) agaATGAATCCCGATGCGCTTACTGTCGTAAGTGATTCAGGTGAAGGAATGCAGCCAACGCAGAGACAg < 1:12904/69‑1 (MQ=38) agaATGAATCCCGATGCGCTTACTGTCGTAAGTGATTCAGGTGAAGGAATGCAGCCAACGCAGAGACAg < 1:751995/69‑1 (MQ=38) agaATGAATCCCGATGCGCTTACTGTCGTAAGTGATTCAGGTGAAGGAATGCAGCCAACGCAGAGACAg < 1:247510/69‑1 (MQ=38) agaATGAATCCCGATGCGCTTACTGTCGTAAGTGATTCAGGTGAAGGAATGCAGCCAACGCAGAGACAg < 1:780215/69‑1 (MQ=38) aatgaatCCCGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAgaga < 1:327278/64‑1 (MQ=255) tgaatCCCGATGCGCTTACTGTCGTAAGTGATTCTGGTGAAGGaa > 1:316690/1‑45 (MQ=255) tgaatCCCGATGCGCTTACTGTCGTAAGTGATTCAGGTGAAGGaa > 1:2121289/1‑45 (MQ=255) tgaatCCCGATGCGCTTACTGTCGTAAGTGATTCAGGTGAAGGaa > 1:1064655/1‑45 (MQ=255) tgaatCCCGATGCGCTTACTGTCGTAAGTGATTCAGGTGAAGGaa > 1:2208510/1‑45 (MQ=255) tgaatCCCGATGCGCTTACTGTCGTAAGTGATTCAGGTGAAGGaa > 1:3010971/1‑45 (MQ=255) tgaatCCCGATGCGCTTACTGTCGTAAGTGATTCAGGTGAAGGaa > 1:2906564/1‑45 (MQ=255) tgaatCCCGATGCGCTTACTGTCGTAAGTGATTCAGGTGAAGGaa > 1:2871977/1‑45 (MQ=255) tgaatCCCGATGCGCTTACTGTCGTAAGTGATTCAGGTGAAGGaa > 1:2658380/1‑45 (MQ=255) tgaatCCCGATGCGCTTACTGTCGTAAGTGATTCAGGTGAAGGaa > 1:1962081/1‑45 (MQ=255) tgaatCCCGATGCGCTTACTGTCGTAAGTGATTCAGGTGAAGGaa > 1:1124526/1‑45 (MQ=255) tgaatCCCGATGCGCTTACTGTCGTAAGTGATTCAGGTGAAGGaa > 1:3402817/1‑45 (MQ=255) tgaatCCCGATGCGCTTACTGTCGTAAGTGATTCAGGTGAAGGaa > 1:173364/1‑45 (MQ=255) tgaatCCCGATGCGCTTACTGTCGTAAGTGATTCAGGTGAAGGaa > 1:1659254/1‑45 (MQ=255) tgaatCCCGATGCGCTTACTGTCGTAAGTGATTCAGGTGAAGGaa > 1:1615603/1‑45 (MQ=255) tgaatCCCGATGCGCTTACTGTCGTAAGTGATTCAGGTGAAGGaa > 1:1171911/1‑45 (MQ=255) tgaatCCCGATGCGCTTACTGTCGTAAGTGATTCAGGTGAAGGaa > 1:499498/1‑45 (MQ=255) tgaatCCCGATGCGCTTACTGTCGTAAGTGATTCAGGTGAAGGaa > 1:3013196/1‑45 (MQ=255) tgaatCCCGATGCGCTTACTGTCGTAAGTGATTCAGGTGAAGGaa > 1:1250758/1‑45 (MQ=255) tgaatCCCGATGCGCTTACTGTCGTAAGTGATTCAGGTGAAGGaa > 1:577971/1‑45 (MQ=255) tgaatCCCGATGCGCTTACTGTCGTAAGTGATTCAGGTGAAGGa > 1:2868018/1‑44 (MQ=38) tCCCGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAGATTGAAg < 1:2356100/68‑1 (MQ=255) tCCCGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAGATTGAAg < 1:410314/68‑1 (MQ=255) tCCCGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAGATTGAAg < 1:1818730/68‑1 (MQ=255) ccGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACa > 1:2849263/1‑58 (MQ=255) ccGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACa > 1:949083/1‑58 (MQ=255) ccGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACa > 1:2651011/1‑58 (MQ=255) ccGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACa > 1:1809457/1‑58 (MQ=255) ccGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACa > 1:2113112/1‑58 (MQ=255) ccGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACa > 1:1310273/1‑58 (MQ=255) ccGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACa > 1:2220045/1‑58 (MQ=255) ccGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACa > 1:1353040/1‑58 (MQ=255) ccGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACa > 1:2403281/1‑58 (MQ=255) ccGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACa > 1:1341376/1‑58 (MQ=255) ccGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAGATTGAAGGa > 1:692307/1‑68 (MQ=255) aGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAGATTGAAGGATGAagag > 1:1127036/1‑69 (MQ=255) aGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAGATTGAAGGATGAagag < 1:1762841/69‑1 (MQ=255) aGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAGATTGAAGGATGAagag > 1:355976/1‑69 (MQ=255) aGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAGATTGAAGGATGAagag < 1:581143/69‑1 (MQ=255) aGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAGATTGAAGGATGAagag < 1:1075338/69‑1 (MQ=255) aGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGAAAGATTGAAGGATGAagag < 1:3367763/69‑1 (MQ=255) agtaAGTGATTGAGGTGTTAGATTGCTGCCTACACAGAGACAGTTTGAAg < 1:1178443/50‑1 (MQ=255) gtaAGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAGATTGAAGGATGAAGAGTATGGGTTcc > 1:585108/1‑68 (MQ=255) gtaAGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAGATTGAAGGATGAAGAGTATGGGTTcc < 1:206323/68‑1 (MQ=255) gtaAGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAGATTGAAGGATGAAGAGTATGGGTTCCa > 1:2898329/1‑69 (MQ=255) gtaAGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAGATTGAAGGATGAAGAGTATGGGTTCCa > 1:498293/1‑69 (MQ=255) gtaAGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAGATTGAAGGATGAAGAGTATGGGTTCCa > 1:22606/1‑69 (MQ=255) gtaAGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAGATTGAAGGATGAAGAGTATGGGTTCCa > 1:2439278/1‑69 (MQ=255) gtaAGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAGATTGAAGGATGAAGAGTATGGGTTCCa > 1:2671338/1‑69 (MQ=255) gtaAGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAGATTGAAGGATGAAGAGTATGGGTTCCa > 1:3192805/1‑69 (MQ=255) aaGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAGATTGAAGGATGAAGAGTATGGGTTCCAcc > 1:1499542/1‑69 (MQ=255) aaGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAGATTGAAGGATGAAGAGTATGGGTTCCAcc > 1:1518460/1‑69 (MQ=255) aaGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAGATTGAAGGATGAAGAGTATGGGTTCCAcc > 1:769451/1‑69 (MQ=255) aaGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAGATTGAAGGATGAAGAGTATGGGTTCCAcc > 1:1030690/1‑69 (MQ=255) aaGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAGATTGAAGGATGAAGAGTATGGGTTCCAcc > 1:335539/1‑69 (MQ=255) aaGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAGATTGAAGGATGAAGAGTATGGGTTCCAcc > 1:2590968/1‑69 (MQ=255) aaGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAGATTGAAGGATGAAGAGTATGGGTTCCAcc > 1:1084056/1‑69 (MQ=255) aaGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAGATTGAAGGATGAAGAGTATGGGTTCCAcc > 1:2696027/1‑69 (MQ=255) aaGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAGATTGAAGGATGAAGAGTATGGGTTCCAc < 1:320093/68‑1 (MQ=255) aaGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAGATTGAAGGATGAAGAGTATGGGTTCCAc > 1:2567441/1‑68 (MQ=255) aaGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAGATTGAAGGATGAAGAGTATGGGTTCCAc < 1:908893/68‑1 (MQ=255) aaGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAGATTGAAGGATGAAGAGTATGGGTTCCAc > 1:2888106/1‑68 (MQ=255) aaGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAGATTGAAGGATGAAGAGTATGGGTTCCAc < 1:2326273/68‑1 (MQ=255) aaGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAGATTGAAGGATGAAGAGTATGGGTTCCAc < 1:2785989/68‑1 (MQ=255) aGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAGATTGAAGGATGAagag > 1:181030/1‑55 (MQ=255) aGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAGATTGAAGGATGAagag > 1:66070/1‑55 (MQ=255) aGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAGATTGAAGGATCAagag > 1:2341955/1‑55 (MQ=255) gTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAGATTGAAg < 1:2896686/45‑1 (MQ=255) gATTCAGGTGAAAGAATGCAGCCAACACAGAGACAGATTGAAGGATGAAGAGTATGGGTTCCACCtcgt > 1:2540678/1‑69 (MQ=255) gTGAAAGAATGCAGCCAACACAGAGACAGATTGAAGGATGAAGAGTATGGGTTCCACCTCGTCGCTGTa < 1:2087654/69‑1 (MQ=255) tGAAAGAATGCAGCCAACACAGAGACAGATTGAAGGATGAagag < 1:1816564/44‑1 (MQ=255) gAAAGAATGCAGCCAACACAGAGACAGATTGAAGGATGAAGAGTATGGGTTCCACCTCGTCGCTGt > 1:2644870/1‑66 (MQ=255) aaaGAATGCAGCCAACACAGAGACAGATTGAAGGATGAAGATTATGGGTTCCACCTCGTCGCTTTATGc > 1:2805540/1‑69 (MQ=255) aaaGAATGCAGCCAACACAGAGACAGATTGAAGGATGAAGAGTATGGGTTCCACCTCGTCGCTGTATGc > 1:851221/1‑69 (MQ=255) aaaGAATGCAGCCAACACAGAGACAGATTGAAGGATGAAGAGTATGGGTTCCACCTCGTCGCTGTATGc > 1:2227478/1‑69 (MQ=255) aaaGAATGCAGCCAACACAGAGACAGATTGAAGGATGAAGAGTATGGGTTCCACCTCGTCGCTGTATGc > 1:2286228/1‑69 (MQ=255) | CAAACTGTATCAAGATGGCAAGAGAATGAATCCCGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAGATTGAAGGATGAAGAGTATGGGTTCCACCTCGTCGCTGTATGC > minE/2434276‑2434409 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |