| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,434,641 | G→A | 39.7% | T86T (ACG→ACA) | gpp → | guanosine pentaphosphatase/exopolyphosphatase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,434,641 | 0 | G | A | 39.7% | 45.8 / 61.9 | 93 | T86T (ACG→ACA) | gpp | guanosine pentaphosphatase/exopolyphosphatase |
| Reads supporting (aligned to +/- strand): ref base G (10/46); new base A (34/3); total (44/49) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.92e-13 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.54e-04 | |||||||||||
TGCGCCTGTTTGCTGAACGTCTGCAAGATATTCCTCCCTCGCAAATTCGCGTTGTCGCTACGGCGACGTTACGCCTTGCCGTCAATGCGGGTGATTTTATTGCCAAAGCGCAGGAAATTCTCGGTTGTCCGG > minE/2434574‑2434705 | tGCGCCTGTTTGCTGAACGTCTGCAAGATATTCCTCCCTCGCAAATTCGCGTTGTCGCTACGGCGACGt < 1:3355834/69‑1 (MQ=255) cTGTTTGCTGAACGTCTGCAAGATATTCCTCCCTCGCAAATTCGCGTTGTCGCTACGGCGACGTTACGc < 1:1964768/69‑1 (MQ=255) cTGTTTGCTGAACGTCTGCAAGATATTCCTCCCTCGCAAATTCGCGTTGTCGCTACGGCGACGTTACGc < 1:3039430/69‑1 (MQ=255) cTGTTTGCTGAACGTCTGCAAGATATTCCTCCCTCGCAAATTCGCGTTGTCGCTACGGCGACGTTACGc < 1:1083084/69‑1 (MQ=255) gAACGTCTGCAAGATATTCCTCCCTCGCAAATTCGCGTTGTCGCTACGGCGACGTTACGCCTTGCCGTc < 1:1026820/69‑1 (MQ=255) gAACGTCTGCAAGATATTCCTCCCTCGCAAATTCGCGTTGTCGCTACGGCGACGTTACGCCTTGCCGTc < 1:285917/69‑1 (MQ=255) gAACGTCTGCAAGATATTCCTCCCTCGCAAATTCGCGTTGTCGCTACGGCGACGTTACGCCTTGCCGTc < 1:3155427/69‑1 (MQ=255) gAACGTCTGCAAGATATTCCTCCCTCGCAAATTCGCGTTGTCGCTACGGCGACGTTACGCCTTGCCGTc < 1:3291549/69‑1 (MQ=255) gAACGTCTGCAAGATATTCCTCCCTCGCAAATTCGCGTTGTCGCTACGGCGACGTTACGCCTTGCCGTc < 1:1217178/69‑1 (MQ=255) gAACGTCTGCAAGATATTCCTCCCTCGCAAATTCGCGTTGTCGCTACGGCGACGTTACGCCTTGCCGTc < 1:1192406/69‑1 (MQ=255) gAACGTCTGCAAGATATTCCTCCCTCGCAAATTCGCGTTGTCGCTACGGCGACGTTACGCCTTGCCGTc < 1:111505/69‑1 (MQ=255) gAACGTCTGCAAGATATTCCTCCCTCGCAAATTCGCGTTGTCGCTACGGCGACGTTACGCCTTGCCGTc < 1:577500/69‑1 (MQ=255) aaCGTCTGCAAGATATTCCTCCCTCGCAAATTCGCGTTGTCGCTACGGCGACGTTACGCCTTGCCGTc < 1:1643931/68‑1 (MQ=255) atccctccctcGCAAATTCGCGTTGTCGCTACGGCGACATTACGCCTTGCCGTCAATGCGGGTGAtttt > 1:507197/4‑69 (MQ=255) atccctccctcGCAAATTCGCGTTGTCGCTACGGCGACATTACGCCTTGCCGTCAATGCGGGTGAtttt > 1:2397332/4‑69 (MQ=255) atccctccctcGCAAATTCGCGTTGTCGCTACGGCGACATTACGCCTTGCCGTCAATGCGGGTGAtttt > 1:2068575/4‑69 (MQ=255) atccctccctcGCAAATTCGCGTTGTCGCTACGGCGACATTACGCCTTGCCGTCAATGCGGGTGAtttt > 1:2026857/4‑69 (MQ=255) atccctccctcGCAAATTCGCGTTGTCGCTACGGCGACATTACGCCTTGCCGTCAATGCGGGTGAtttt > 1:1855144/4‑69 (MQ=255) atccctccctcGCAAATTCGCGTTGTCGCTACGGCGACATTACGCCTTGCCGTCAATGCGGGTGAtttt > 1:1000313/4‑69 (MQ=255) atccctccctcGCAAATTCGCGTTGTCGCTACGGCGACATTACGCCTTGCCGTCAATGCGGGTGAtttt > 1:176249/4‑69 (MQ=255) atccctccctcGCAAATTCGCGTTGTCGCTACGGCGACATTACGCCTTGCCGTCAATGCGGGTGAtttt > 1:3034275/4‑69 (MQ=255) atccctccctcGCAAATTCGCGTTGTCGCTACGGCGACATTACGCCTTGCCGTCAATGCGGGTGAtttt > 1:1570737/4‑69 (MQ=255) atccctccctcGCAAATTCGCGTTGTCGCTACGGCGACATTACGCCTTGCCGTCAATGCGGGTGAtttt > 1:3036048/4‑69 (MQ=255) atccctccctcGCAAATTCGCGTTGTCGCTACGGCGACATTACGCCTTGCCGTCAATGCGGGTGAtttt > 1:1371214/4‑69 (MQ=255) atccctccctcGCAAATTCGCGTTGTCGCTACGGCGACATTACGCCTTGCCGTCAATGCGGGTGAtttt > 1:1243651/4‑69 (MQ=255) atccctccctcGCAAATTCGCGTTGTCGCTACGGCGACATTACGCCTTGCCGTCAATGCGGGTGAttt > 1:2773677/4‑68 (MQ=255) atccctccctcGAAAATTCGCGTTGTCGCTACGGCGACATTACGCCTTGCCGTCAATGCGGGTGAtttt > 1:3017405/4‑69 (MQ=255) ttCCTCCCTCGCAAATTCGCGTTGTCGCTACGGCGACGTTACGCCTTGCCGTc < 1:3015373/53‑1 (MQ=255) ttCCTCCCTCGCAAATTCGCGTTGTCGCTACGGCGACGTTACGCCTTGCCGTc < 1:965768/53‑1 (MQ=255) ttCCTCCCTCGCAAATTCGCGTTGTCGCTACGGCGACGTTACGCCTTGCCGTc < 1:969817/53‑1 (MQ=255) ttCCTCCCTCGCAAATTCGCGTTGTCGCTACGGCGACGTTACGCCTTGCCGTc < 1:2607852/53‑1 (MQ=255) ttCCTCCCTCGCAAATTCGCGTTGTCGCTACGGCGACGTTACGCCTTGCCGTc < 1:2439320/53‑1 (MQ=255) ttCCTCCCTCGCAAATTCGCGTTGTCGCTACGGCGACGTTACGCCTTGCCGTc < 1:3381289/53‑1 (MQ=255) ttCCTCCCTCGCAAATTCGCGTTGTCGCTACGGCGACGTTACGCCTTGCCGTc < 1:1036561/53‑1 (MQ=255) ttCCTCCCTCGCAAATTCGCGTTGTCGCTACGGCGACGTTACGCCTTGCCGTc < 1:1122109/53‑1 (MQ=255) ttCCTCCCTCGCAAATTCGCGTTGTCGCTACGGCGACGTTACGCCTTGCCGTCAATGCGGGTGATTTTa < 1:1032970/69‑1 (MQ=255) ttCCTCCCTCGCAAATTCGCGTTGTCGCTACGGCGACGTTACGCCTTGCCGTCAATGCGGGTGATTTTa < 1:218445/69‑1 (MQ=255) ttCCTCCCTCGCAAATTCGCGTTGTCGCTACGGCGACGTTACGCCTTGCCGTCAATGCGGGTGATTTTa < 1:908610/69‑1 (MQ=255) ttCCTCCCTCGCAAATTCGCGTTGTCGCTACGGCGACGTTACGCCTTGCCGTCAATGCGGGTGATTTTa < 1:1141638/69‑1 (MQ=255) ttCCTCCCTCGCAAATTCGCGTTGTCGCTACGGCGACGTTACGCCTTGCCGTCAATGCGGGTGATTTTa < 1:2103230/69‑1 (MQ=255) ttCCTCCCTCGCAAATTCGCGTTGTCGCTACGGCGACGTTACGCCTTGCCGTCAATGCGGGTGATTTTa < 1:1249770/69‑1 (MQ=255) ttCCTCCCTCGCAAATTCGCGTTGTCGCTACGGCGACGTTACGCCTTGCCGTCAATGCGGGTGATTTTa < 1:2402493/69‑1 (MQ=255) ttCCTCCCTCGCAAATTCGCGTTGTCGCTACGGCGACGTTACGCCTTGCCGTCAATGCGGGTGATTTTa < 1:1399987/69‑1 (MQ=255) ttCCTCCCTCGCAAATTCGCGTTGTCGCTACGGCGACGTTACGCCTTGCCGTCAATGCGGGTGATTTTa < 1:1773677/69‑1 (MQ=255) ttCCTCCCTCGCAAATTCGCGTTGTCGCTACGGCGACGTTACGCCTTGCCGTCAATGCGGGTGATTTTa < 1:1799570/69‑1 (MQ=255) ttCCTCCCTCGCAAATTCGCGTTGTCGCTACGGCGACGTTACGCCTTGCCGTCAATGCGGGTGATTTTa < 1:2669555/69‑1 (MQ=255) ttCCTCCCTCGCAAATTCGCGTTGTCGCGACGGCGACGTTACGCCTTGCCGTCAATGCGGGTGATTTTa < 1:2146678/69‑1 (MQ=255) ttCATCCCTCGCAAATTCGCGTTGTCGCTACGGCGACGTTACGCCTTGCCGTc < 1:3146467/53‑1 (MQ=255) tcctccCTCGCAAATTCGCGTTGTCGCTACGGCGACGTTACGCCTTGCCGTCAATGCGGGTGATTTTa < 1:2066244/68‑1 (MQ=255) cctccctcGCAAATTCGCGTTGTCGCTACGGCGACGTTACGCCTTGCCGTCAATGCGGGTGATTTTAtt > 1:590033/1‑69 (MQ=255) cctccctcGCAAATTCGCGTTGTCGCTACGGCGACGTTACGCCTTGCCGTCAATGCGGGTGATTTTAtt > 1:558890/1‑69 (MQ=255) cctccctcGCAAATTCGCGTTGTCGCTACGGCGACGTTACGCCTTGCCGTCAATGCGGGTGATTTTAtt > 1:2287250/1‑69 (MQ=255) cctccctcGCAAATTCGCGTTGTCGCTACGGCGACGTTACGCCTTGCCGTCAATGCGGGTGATTTTAtt > 1:2785458/1‑69 (MQ=255) cctccctcGCAAATTCGCGTTGTCGCTACGGCGACGTTACGCCTTGCCGTCAATGCGGGTGATTTTAtt > 1:3049204/1‑69 (MQ=255) cctccctcGCAAATTCGCGTTGTCGCTACGGCGACGTTACGCCTTGCCGTCAATGCGGGTGATTTTAt > 1:2400961/1‑68 (MQ=255) tccctcGCAAATTCGCGTTGTCGCTACGGCGACATTACGCCTTGCCGTCAATGc > 1:2033006/1‑54 (MQ=255) tccctcGCAAATTCGCGTTGTCGCTACGGCGACATTACGCCTTGCCGTCAATGCGGGTGATTTTATTg > 1:1275705/1‑68 (MQ=255) tccctcGCAAATTCGCGTTGTCGCTACGGCGACATTACGCCTTGCCGTCAATGCGGGTGATTTTATTg > 1:1107650/1‑68 (MQ=255) tccctcGCAAATTCGCGTTGTCGCTACGGCGACATTACGCCTTGCCGTCAATGCGGGTGATTTTATTg > 1:2579867/1‑68 (MQ=255) tccctcGCAAATTCGCGTTGTCGCTACGGCGACATTACGCCTTGCCGTCAATGCGGGTGATTTTATTg > 1:708447/1‑68 (MQ=255) tccctcGCAAATTCGCGTTGTCGCTACGGCGACATTACGCCTTGCCGTCAATGCGGGTGATTTTATTg > 1:766330/1‑68 (MQ=255) tccctcGCAAATTCGCGTTGTCGCTACGGCGACATTACGCCTTGCCGTCAATGCGGGTGATTTTATTg > 1:1139984/1‑68 (MQ=255) tccctcGCAAATTCGCGTTGTCGCTACGGCGACATTACGCCTTGCCGTCAATGCGGGTGATTTTATTg > 1:2527544/1‑68 (MQ=255) tccctcGCAAATTCGCGTTGTCGCTACGGCGACATTACGCCTTGCCGTCAATGCGGGTGATTTTATTg > 1:2758566/1‑68 (MQ=255) tccctcGCAAATTCGCGTTGTCGCTACGGCGACATTACGCCTTGCCGTCAATGCGGGTGATTTTATTg > 1:496511/1‑68 (MQ=255) tccctcGCAAATTCGCGTTGTCGCTACGGCGACATTACGCCTTGCCGTCAATGCGGGTGATTTTATTg > 1:3257031/1‑68 (MQ=255) tccctcGCAAATTCGCGTTGTCGCTACGGCGACATTACGCCTTGCCGTCAATGCGGGTGATTTTATTg > 1:2510025/1‑68 (MQ=255) tccctcGCAAATTCGCGTTGTCGCTACGGCGACATTACGCCTTGCCGTCAATGCGGGTGATTTTATTg > 1:3073843/1‑68 (MQ=255) tccctcGCAAATTCGCGTTGTCGCTACGGCGACATTACGCCTTGCCGTCAATGCGGGTGATTTTATTg > 1:2507693/1‑68 (MQ=255) tccctcGCAAATTCGCGTTGTCGCTACGGCGACATTACGCCTTGCCGTCAATGCGGGTGATTTTATTg > 1:3039970/1‑68 (MQ=255) tccctcGCAAATTCGCGTTGTCGCTACGGCGACATTACGCCTTGCCGTCAATGCGGGTGATTTTATTg > 1:1564757/1‑68 (MQ=255) tccctcGCAAATTCGCGTTGTCGCTACGGCGACATTACGCCTTGCCGTCAATGCGGGTGATTTTATTg > 1:1624603/1‑68 (MQ=255) tccctcGCAAATTCGCGTTGTCGCTACGGCGACATTACGCCTTGCCGTCAATGCGGGTGATTTTATTg > 1:2166693/1‑68 (MQ=255) tccctcGCAAATTCGCGTTGTCGCTACGGCGACATTACGCCTTGCCGTCAATGCGGGTGATTTTATTg > 1:222414/1‑68 (MQ=255) tccctcGCAAATTCGCGTTGTCGCTACGGCGACATTACGCCTTGCCGGCTATGCGGGTGATTTTATTg > 1:803358/1‑68 (MQ=255) cAAATTCGCGTTGTCGCTACGGCGACATTACGCCTTGCCGTCAATGCGGGTGATTTTATTGCCa < 1:3272468/64‑1 (MQ=255) cAAATTCGCGTTGTCGCTACGGCGACATTACGCCTTGCCGTCAATGCGGGTGATTTTATTGCCa < 1:557315/64‑1 (MQ=255) cAAATTCGCGTTGTCGCTACGGCGACATTACGCCTTGCCGTCAATGCGGGTGATTTTATTGCCa < 1:36137/64‑1 (MQ=255) cGCTACGGCGACGTTACGCCTTGCCGTCAATGCGGGTGATTTTATTGCCAAAGCGCAGGAAATTCTCgg > 1:1674797/1‑69 (MQ=255) cGCTACGGCGACGTTACGCCTTGCCGTCAATGCGGGTGATTTTATTGCCAAAGCGCAGGAAATTCTCgg > 1:2131389/1‑69 (MQ=255) cGCTACGGCGACGTTACGCCTTGCCGTCAATGCGGGTGATTTTATTGCCAAAGCGCAGGAAATTCTCgg > 1:2946277/1‑69 (MQ=255) cGCTACGGCGACGTTACGCCTTGCCGTCAATGCGGGTGATTTTATTGCCAAAGCGCAGGAAATTCTCgg > 1:2954405/1‑69 (MQ=255) gCGACGTTACGCCTTGCCGTCAATGCGGGTGATTTTATTGCCAAAGCGCAGGAAATTCTCGGTTGTcc < 1:78593/68‑1 (MQ=255) cGACGTTACGCCTTGCCGTCAATGCGGGTGATTTTATTGCCAAAGCGCAGGAAATTCTCGGTTGTCCgg < 1:464720/69‑1 (MQ=255) cGACGTTACGCCTTGCCGTCAATGCGGGTGATTTTATTGCCAAAGCGCAGGAAATTCTCGGTTGTCCgg < 1:2665511/69‑1 (MQ=255) cGACGTTACGCCTTGCCGTCAATGCGGGTGATTTTATTGCCAAAGCGCAGGAAATTCTCGGTTGTCCgg < 1:419402/69‑1 (MQ=255) cGACGTTACGCCTTGCCGTCAATGCGGGTGATTTTATTGCCAAAGCGCAGGAAATTCTCGGTTGTCCgg < 1:414848/69‑1 (MQ=255) cGACGTTACGCCTTGCCGTCAATGCGGGTGATTTTATTGCCAAAGCGCAGGAAATTCTCGGTTGTCCgg < 1:626459/69‑1 (MQ=255) cGACGTTACGCCTTGCCGTCAATGCGGGTGATTTTATTGCCAAAGCGCAGGAAATTCTCGGTTGTCCgg < 1:414800/69‑1 (MQ=255) cGACGTTACGCCTTGCCGTCAATGCGGGTGATTTTATTGCCAAAGCGCAGGAAATTCTCGGTTGTCCgg < 1:708865/69‑1 (MQ=255) cGACGTTACGCCTTGCCGTCAATGCGGGTGATTTTATTGCCAAAGCGCAGGAAATTCTCGGTTGTCCgg < 1:3418833/69‑1 (MQ=255) cGACGTTACGCCTTGCCGTCAATGCGGGTGATTTTATTGCCAAAGCGCAGGAAATTCTCGGTTGTCCgg < 1:2197571/69‑1 (MQ=255) cGACGTTACGCCTTGCCGTCAATGCGGGTGATTTTATTGCCAAAGCGCAGGAAATTCTCGGTTGTCCgg < 1:1649410/69‑1 (MQ=255) | TGCGCCTGTTTGCTGAACGTCTGCAAGATATTCCTCCCTCGCAAATTCGCGTTGTCGCTACGGCGACGTTACGCCTTGCCGTCAATGCGGGTGATTTTATTGCCAAAGCGCAGGAAATTCTCGGTTGTCCGG > minE/2434574‑2434705 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |