| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,439,549 | C→T | 44.8% | V365V (GTG→GTA) | ilvC ← | ketol‑acid reductoisomerase, NAD(P)‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,439,549 | 0 | C | T | 44.8% | 24.3 / 88.7 | 110 | V365V (GTG→GTA) | ilvC | ketol‑acid reductoisomerase, NAD(P)‑binding |
| Reads supporting (aligned to +/- strand): ref base C (8/52); new base T (35/14); total (43/67) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.21e-10 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.42e-01 | |||||||||||
Only 100 of 110 total aligned reads displayed.
ATATGCAGACTCTTCAATGATGCCGGAATCGACCATGGTTTCGAACGCCAGTTCAACGCCCGCTTTCACCATCGCAATCATCAGTACGCCTTTATCGAAGTACTCCTGCTCGCCGATTTTGCCTTCAT > minE/2439483‑2439610 | atatGCAGACTCTTCAATGATGCCGGAATCGACCATGGTTTCGAACGCCAGTTCAACGCCCGCTTTCAc < 1:366522/69‑1 (MQ=255)atatGCAGACTCTTCAATGATGCCGGAATCGACCATGGTTTCGAACGCCAGTTCAACGCCCGCTTTCAc < 1:179676/69‑1 (MQ=255)atatGCAGACTCTTCAATGATGCCGGAATCGACCATGGTTTCGAACGCCAGTTCAACGCCCGCTTTCAc < 1:2117208/69‑1 (MQ=255)atatGCAGACTCTTCAATGATGCCGGAATCGACCATGGTTTCGAACGCCAGTTCAACGCCCGCTTTCAc < 1:2305574/69‑1 (MQ=255)atatGCAGACTCTTCAATGATGCCGGAATCGACCATGGTTTCGAACGCCAGTTCAACGCCCGCTTTCAc < 1:2560891/69‑1 (MQ=255)atatGCAGACTCTTCAATGATGCCGGAATCGACCATGGTTTCGAACGCCAGTTCAACGCCCGCTTTCAc < 1:2571453/69‑1 (MQ=255)atatGCAGACTCTTCAATGATGCCGGAATCGACCATGGTTTCGAACGCCAGTTCAACGCCCGCTTTCAc < 1:2714750/69‑1 (MQ=255)atatGCAGACTCTTCAATGATGCCGGAATCGACCATGGTTTCGAACGCCAGTTCAACGCCCGCTTTCAc < 1:2815239/69‑1 (MQ=255)atatGCAGACTCTTCAATGATGCCGGAATCGACCATGGTTTCGAACGCCAGTTCAACGCCCGCTTTCAc < 1:2949008/69‑1 (MQ=255)atatGCAGACTCTTCAATGATGCCGGAATCGACCATGGTTTCGAACGCCAGTTCAACGCCCGCTTTCAc < 1:1079359/69‑1 (MQ=255)atatGCAGACTCTTCAATGATGCCGGAATCGACCATGGTTTCGAACGCCAGTTCAACGCCCGCTTTCAc < 1:429914/69‑1 (MQ=255)atatGCAGACTCTTCAATGATGCCGGAATCGACCATGGTTTCGAACGCCAGTTCAACGCCCGCTTTCAc < 1:466753/69‑1 (MQ=255)atatGCAGACTCTTCAATGATGCCGGAATCGACCATGGTTTCGAACGCCAGTTCAACGCCCGCTTTCAc < 1:592625/69‑1 (MQ=255)atatGCAGACTCTTCAATGATGCCGGAATCGACCATGGTTTCGAACGCCAGTTCAACGCCCGCTTTCAc < 1:708072/69‑1 (MQ=255)atatGCAGACTCTTCAATGATGCCGGAATCGACCATGGTTTCGAACGCCAGTTCAACGCCCGCTTTCAc < 1:789300/69‑1 (MQ=255)atatGCAGACTCTTCAATGATGCCGGAATCGACCATGGTTTCGAACGCCAGTTCAACGCCCGCTTTCAc < 1:863577/69‑1 (MQ=255)atatGCAGACTCTTCAATGATGCCGGAATCGACCATGGTTTCGAACGCCAGTTCAACGCCCGCTTTCAc < 1:880250/69‑1 (MQ=255)atatGCAGACTCTTCAATGATGCCGGAATCGACCATGGTTTCGAACGCCAGTTCAACGCCCGCTTTCAc < 1:1289354/69‑1 (MQ=255)atatGCAGACTCTTCAATGATGCCGGAATCGACCATGGTTTCGAACGCCAGTTCAACGCCCGCTTTCAc < 1:1284325/69‑1 (MQ=255)atatGCAGACTCTTCAATGATGCCGGAATCGACCATGGTTTCGAACGCCAGTTCAACGCCCGCTTTCAc < 1:1410896/69‑1 (MQ=255)atatGCAGACTCTTCAATGATGCCGGAATCGACCATGGTTTCGAACGCCAGTTCAACGCCCGCTTTCAc < 1:1171758/69‑1 (MQ=255)atatGCAGACTCTTCAATGATGCCGGAATCGACCATGGTTTCGAACGCCAGTTCAACGCCCGCTTTAAc < 1:114885/69‑1 (MQ=255) tGCAGACTCTTCAATGATGCCGGAATCGACCATGGTTTCGAACGCCAGTTCAACGCCCGCTTTCAc < 1:72857/66‑1 (MQ=255) cAGACTCTTCAATGATGCCGGAATCGACCATGGTTTCGAACGCCAGTTCAACGCCCGCTTTCACCATc > 1:2676521/1‑68 (MQ=255) tctTCGATGATGCCGGAATCAACCATGGTTTCAAACTCCAACTCAACGCCTGCTTTTACCATCGCGatc > 1:1684764/1‑69 (MQ=255) tctTCGATGATGCCGGAATCAACCATGGTTTCAAACGCCAACTCAACGCCTGCTTTTACCATCGCGatc > 1:1111401/1‑69 (MQ=255) tctTCGATGATGCCGGAATCAACCATGGTTTCAAACGCCAACTCAACGCCTGCTTTTACCATCGCGatc > 1:744707/1‑69 (MQ=255) tctTCGATGATGCCGGAATCAACCATGGTTTCAAACGCCAACTCAACGCCTGCTTTTACCATCGCGatc > 1:753982/1‑69 (MQ=255) tctTCGATGATGCCGGAATCAACCATGGTTTCAAACGCCAACTCAACGCCTGCTTTTACCATCGCGatc > 1:1262569/1‑69 (MQ=255) tctTCGATGATGCCGGAATCAACCATGGTTTCAAACGCCAACTCAACGCCTGCTTTTACCATCGCGatc > 1:103095/1‑69 (MQ=255) tctTCGATGATGCCGGAATCAACCATGGTTTCAAACGCCAACTCAACGCCTGCTTTTACCATCGCGatc > 1:2402000/1‑69 (MQ=255) tctTCGATGATGCCGGAATCAACCATGGTTTCAAACGCCAACTCAACGCCTGCTTTTACCATCGCGatc > 1:3077571/1‑69 (MQ=255) tctTCGATGATGCCGGAATCAACCATGGTTTCAAACGCCAACTCAACGCCTGCTTTTACCATCGCGatc > 1:2519116/1‑69 (MQ=255) tctTCAATGATGCCGGAATCGACCATGGTTTCGAACGCCAGTTCAACGCCCGCTTTCACCATCGCAatc > 1:3144318/1‑69 (MQ=255) tctTCAATGATGCCGGAATCGACCATGGTTTCGAACGCCAGTTCAACGCCCGCTTTCACCATCGCAatc > 1:135680/1‑69 (MQ=255) tctTCAATGATGCCGGAATCGACCATGGTTTCGAACGCCAGTTCAACGCCCGCTTTCACCATCGCAat > 1:3181218/1‑68 (MQ=255) atgatgCCGGAATCGAGCATGGTTTCGAACGCCAGTTCAACGCCCGCTTTCACCATCGCAatcatc < 1:2956986/66‑1 (MQ=255) atgatgCCGGAATCGACCATGGTTTCGAACGCCAGTTCAACGCCCGCTTTCACCATCGCAatcatc < 1:1325765/66‑1 (MQ=255) atgatgCCGGAATCGACCATGGTTTCGAACGCCAGTTCAACGCCCGCTTTCACCATCGCAatcatc < 1:1350197/66‑1 (MQ=255) atgatgCCGGAATCGACCATGGTTTCGAACGCCAGTTCAACGCCCGCTTTCACCATCGCAatcatc < 1:274342/66‑1 (MQ=255) atgatgCCGGAATCGACCATGGTTTCGAACGCCAGTTCAACGCCCGCTTTCACCATCGCAatcatc < 1:3168586/66‑1 (MQ=255) atgatgCCGGAATCGACCATGGTTTCGAACGCCAGTTCAACGCCCGCTTTCACCATCGCAatcatc < 1:1546740/66‑1 (MQ=255) atgatgCCGGAATCGACCATGGTTTCGAACGCCAGTTCAACGCCCGCTTTCACCATCGCAatcatc < 1:751958/66‑1 (MQ=255) atgatgCCGGAATCGACCATGGTTTCGAACGCCAGTTCAACGCCCGCTTTCACCATCGCAatcatc < 1:1773915/66‑1 (MQ=255) atgatgCCGGAATCGACCATGGTTTCGAACGCCAGTTCAACGCCCGCTTTCACCATCGCAatcatc < 1:847354/66‑1 (MQ=255) atgatgCCGGAATCGACCATGGTTTCGAACGCCAGTTCAACGCCCGCTTTCACCATCGCAatcatc < 1:1423978/66‑1 (MQ=255) atgatgCCGGAATCGACCATGGTTTCGAACGCCAGTTCAACGCCCGCTTTCACCATCGCAATCATCAg < 1:747094/68‑1 (MQ=255) atgatgCCGGAATCGACCATGGTTTCGAACGCCAGTTCAACGCCCGCTTTCACCATCGCAATCATCAg < 1:3185525/68‑1 (MQ=255) tgatgCCGGAATCGACCATGGTTTCGAACGCCAGTTCAACGCCCGCTTTCACCATCGCAATCATCAGTa < 1:1643091/69‑1 (MQ=255) gatgCCGGAATCGACCATGGTTTCGAACGCCAGTTCAACGCCCGCTTTCACCATCGCAATCATCAGTa < 1:1973441/68‑1 (MQ=255) gatgCCGGAATCAACCATGGTTTCAAACGCCAACTCAACGCCTGCTTTTACCATCGCGATCATCAGTAc > 1:2987486/1‑69 (MQ=255) gatgCCGGAATCAACCATGGTTTCAAACGCCAACTCAACGCCTGCTTTTACCATCGCGATCATCAGTAc > 1:662912/1‑69 (MQ=255) gatgCCGGAATCAACCATGGTTTCAAACGCCAACTCAACGCCTGCTTTTACCATCGCGATCATCAGTAc > 1:950326/1‑69 (MQ=255) gatgCCGGAATCAACCATGGTTTCAAACGCCAACTCAACGCCTGCTTTTACCATCGCGATCATCAGTAc > 1:885345/1‑69 (MQ=255) gatgCCGGAATCAACCATGGTTTCAAACGCCAACTCAACGCCTGCTTTTACCATCGCGATCATCAGTAc > 1:3256387/1‑69 (MQ=255) gatgCCGGAATCAACCATGGTTTCAAACGCCAACTCAACGCCTGCTTTTACCATCGCGATCATCAGTAc > 1:447391/1‑69 (MQ=255) gatgCCGGAATCAACCATGGTTTCAAACGCCAACTCAACGCCTGCTTTTACCATCGCGATCATCAGTAc > 1:567570/1‑69 (MQ=255) gatgCCGGAATCAACCATGGTTTCAAACGCCAACTCAACGCCTGCTTTTACCATCGCGATCATCAGTAc > 1:822403/1‑69 (MQ=255) gatgCCGGAATCAACCATGGTTTCAAACGCCAACTCAACGCCTGCTTTTACCATCGCGATCATCAGTAc > 1:595857/1‑69 (MQ=255) gatgCCGGAATCAACCATGGTTTCAAACGCCAACTCAACGCCTGCTTTTACCATCGCGATCATCAGTAc > 1:64500/1‑69 (MQ=255) gatgCCGGAATCAACCATGGTTTCAAACGCCAACTCAACGCCTGCTTTTACCATCGCGATCATCAGTAc > 1:722120/1‑69 (MQ=255) gatgCCGGAATCAACCATGGTTTCAAACGCCAACTCAACGCCTGCTTTTACCATCGCGATCATCAGTAc > 1:1149744/1‑69 (MQ=255) gatgCCGGAATCAACCATGGTTTCAAACGCCAACTCAACGCCTGCTTTTACCATCGCGATCATCAGTAc > 1:1557789/1‑69 (MQ=255) gatgCCGGAATCAACCATGGTTTCAAACGCCAACTCAACGCCTGCTTTTACCATCGCGATCATCAGTAc > 1:1811497/1‑69 (MQ=255) gatgCCGGAATCAACCATGGTTTCAAACGCCAACTCAACGCCTGCTTTTACCATCGCGATCATCAGTAc > 1:1882685/1‑69 (MQ=255) gatgCCGGAATCAACCATGGTTTCAAACGCCAACTCAACGCCTGCTTTTACCATCGCGATCATCAGTAc > 1:1422337/1‑69 (MQ=255) gatgCCGGAATCAACCATGGTTTCAAACGCCAACTCAACGCCTGCTTTTACCATCGCGATCATCAGTAc > 1:2143389/1‑69 (MQ=255) gatgCCGGAATCAACCATGGTTTCAAACGCCAACTCAACGCCTGCTTTTACCATCGCGATCATCAGTAc > 1:21591/1‑69 (MQ=255) gatgCCGGAATCAACCATGGTTTCAAACGCCAACTCAACGCCTGCTTTTACCATCGCGATCATCAGTAc > 1:246220/1‑69 (MQ=255) gatgCCGGAATCAACCATGGTTTCAAACGCCAACTCAACGCCTGCTTTTACCATCGCGATCATCAGTAc > 1:2571512/1‑69 (MQ=255) gatgCCGGAATCAACCATGGTTTCAAACGCCAACTCAACGCCTGCTTTTACCATCGCGATCATCAGTAc > 1:2582695/1‑69 (MQ=255) gatgCCGGAATCAACCATGGTTTCAAACGCCAACTCAACGCCTGCTTTTACCATCGCGATCATCAGTAc > 1:2761832/1‑69 (MQ=255) gatgCCGGAATCAACCATGGTTTCAAACGCCAACTCAACGCCTGCTTTTACCATCGCGATCATCAGTAc > 1:1321545/1‑69 (MQ=255) gatgCCGGAATCAACAATGGTTTCAAACGCCAACTCAACGCCTGCTTTTACCATCGCGATCATCAGTAc > 1:3226208/1‑69 (MQ=255) ggAATCGACCATGGTTTCGAACGCCAGTTCAACGCCCGCTTTCACCATCGCAatcatc < 1:2855503/58‑1 (MQ=255) ccATGGTTTCGAACGCCAGTTCAACGCCCGCTTTCACCATCGCAATCATCAGTACGCCTTTATCGAAg < 1:2931062/68‑1 (MQ=255) ccATGGTTTCGAACGCCAGTTCAACGCCCGCTTTCACCATCGCAATCATCAGTACGCCTTTATCGAAg < 1:63579/68‑1 (MQ=255) ccATGGTTTCGAACGCCAGTTCAACGCCCGCTTTCACCATCGCAATCATCAGTACGCCTTTATCGAAg < 1:2829540/68‑1 (MQ=255) ccATGGTTTCGAACGCCAGTTCAACGCCCGCTTTCACCATCGCAATCATCAGTACGCCTTTATCGAAg < 1:451198/68‑1 (MQ=255) ccATGGTTTCGAACGCCAGTTCAACGCCCGCTTTCACCATCGCAATCATCAGTACGCCTTTATCGAAg < 1:1268706/68‑1 (MQ=255) gTTTCGAACGCCAGTTCAACGCCCGCTTTCACCATCGCAATCATCAGTACGCCTTTATCGAAGTACTcc > 1:354062/1‑69 (MQ=255) gTTTCGAACGCCAGTTCAACGCCCGCTTTCACCATCGCAATCATCAGTACGCCTTTATCGAAGTACTcc > 1:3405036/1‑69 (MQ=255) tCGAACGCCAGTTCAACGCCCGCTTTCACCATCGCAATCAT‑AGTACGCCTTTATCGAAGTACTCCTGCt > 1:454727/1‑69 (MQ=255) cGCCAGTTCAACGCCCGCTTTCACCATCGCAATCATCAGTa < 1:531034/41‑1 (MQ=255) cccGCTTTCACCATCGCAATCATCAGTACGCCTTTATCGAAGTACTCCTGCTCGCCGATTTTGCCTTCa < 1:2303700/69‑1 (MQ=255) cccGCTTTCACCATCGCAATCATCAGTACGCCTTTATCGAAGTACTCCTGCTCGCCGATTTTGCCTTCa < 1:1714603/69‑1 (MQ=255) ctgCTTTTACCATCGCGATCATCAGTACGCCTTTATCGAAGTACTCCTGTTCACCGATTTTGCCTTCAt < 1:1507216/67‑1 (MQ=255) ctgCTTTTACCATCGCGATCATCAGTACGCCTTTATCGAAGTACTCCTGTTCACCGATTTTGCCTTCAt < 1:1449830/67‑1 (MQ=255) ctgCTTTTACCATCGCGATCATCAGTACGCCTTTATCGAAGTACTCCTGTTCACCGATTTTGCCTTCAt < 1:2908527/67‑1 (MQ=255) ctgCTTTTACCATCGCGATCATCAGTACGCCTTTATCGAAGTACTCCTGTTCACCGATTTTGCCTTCAt < 1:861423/67‑1 (MQ=255) ctgCTTTTACCATCGCGATCATCAGTACGCCTTTATCGAAGTACTCCTGTTCACCGATTTTGCCTTCAt < 1:3372345/67‑1 (MQ=255) ctgCTTTTACCATCGCGATCATCAGTACGCCTTTATCGAAGTACTCCTGTTCACCGATTTTGCCTTCAt < 1:1973008/67‑1 (MQ=255) ctgCTTTTACCATCGCGATCATCAGTACGCCTTTATCGAAGTACTCCTGTTCACCGATTTTGCCTTCAt < 1:2132531/67‑1 (MQ=255) ctgCTTTTACCATCGCGATCATCAGTACGCCTTTATCGAAGTACTCCTGTTCACCGATTTTGCCTTCAt < 1:751126/67‑1 (MQ=255) ctgCTTTTACCATCGCGATCATCAGTACGCCTTTATCGAAGTACTCCTGTTCACCGATTTTGCCTTCAt < 1:2884817/67‑1 (MQ=255) ctgCTTTTACCATCGCGATCATCAGTACGCCTTTATCGAAGTACTCCTGTTCACCGATTTTGCCTTCAt < 1:2349725/67‑1 (MQ=255) ctgCTTTTACCATCGCGATCATCAGTACGCCTTTATCGAAGTACTCCTGTTCACCGATTTTGCCTTCAt < 1:430240/67‑1 (MQ=255) ccGCTTTCACCATCGCAATCATCAGTACGCCTTTATCGAAGTACTCCTGCTCGCCGATTTTGCCTTCAt < 1:857752/69‑1 (MQ=255) ccGCTTTCACCATCGCAATCATCAGTACGCCTTTATCGAAGTACTCCTGCTCGCCGATTTTGCCTTCAt < 1:2205888/69‑1 (MQ=255) tgCTTTTACCATCGCGATCATCAGTACGCCTTTATCGAAGTACTCCTGTTCACCGATTTTGCCTTCAt < 1:2055025/67‑1 (MQ=255) | ATATGCAGACTCTTCAATGATGCCGGAATCGACCATGGTTTCGAACGCCAGTTCAACGCCCGCTTTCACCATCGCAATCATCAGTACGCCTTTATCGAAGTACTCCTGCTCGCCGATTTTGCCTTCAT > minE/2439483‑2439610 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |