| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,439,558 | A→G | 60.5% | I362I (ATT→ATC) | ilvC ← | ketol‑acid reductoisomerase, NAD(P)‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,439,558 | 0 | A | G | 60.5% | 42.0 / 57.9 | 86 | I362I (ATT→ATC) | ilvC | ketol‑acid reductoisomerase, NAD(P)‑binding |
| Reads supporting (aligned to +/- strand): ref base A (7/27); new base G (35/17); total (42/44) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.47e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01 | |||||||||||
TCTTCAATGATGCCGGAATCGACCATGGTTTCGAACGCCAGTTCAACGCCCGCTTTCACCATCGCAATCATCAGTACGCCTTTATCGAAGTACTCCTGCTCGCCGATTTTGCCTTCATACTGCGGCGCG > minE/2439493‑2439621 | tctTCGATGATGCCGGAATCAACCATGGTTTCAAACTCCAACTCAACGCCTGCTTTTACCATCGCGatc > 1:1684764/1‑69 (MQ=255)tctTCGATGATGCCGGAATCAACCATGGTTTCAAACGCCAACTCAACGCCTGCTTTTACCATCGCGatc > 1:1111401/1‑69 (MQ=255)tctTCGATGATGCCGGAATCAACCATGGTTTCAAACGCCAACTCAACGCCTGCTTTTACCATCGCGatc > 1:3077571/1‑69 (MQ=255)tctTCGATGATGCCGGAATCAACCATGGTTTCAAACGCCAACTCAACGCCTGCTTTTACCATCGCGatc > 1:103095/1‑69 (MQ=255)tctTCGATGATGCCGGAATCAACCATGGTTTCAAACGCCAACTCAACGCCTGCTTTTACCATCGCGatc > 1:2519116/1‑69 (MQ=255)tctTCGATGATGCCGGAATCAACCATGGTTTCAAACGCCAACTCAACGCCTGCTTTTACCATCGCGatc > 1:2402000/1‑69 (MQ=255)tctTCGATGATGCCGGAATCAACCATGGTTTCAAACGCCAACTCAACGCCTGCTTTTACCATCGCGatc > 1:744707/1‑69 (MQ=255)tctTCGATGATGCCGGAATCAACCATGGTTTCAAACGCCAACTCAACGCCTGCTTTTACCATCGCGatc > 1:753982/1‑69 (MQ=255)tctTCGATGATGCCGGAATCAACCATGGTTTCAAACGCCAACTCAACGCCTGCTTTTACCATCGCGatc > 1:1262569/1‑69 (MQ=255)tctTCAATGATGCCGGAATCGACCATGGTTTCGAACGCCAGTTCAACGCCCGCTTTCACCATCGCAatc > 1:135680/1‑69 (MQ=255)tctTCAATGATGCCGGAATCGACCATGGTTTCGAACGCCAGTTCAACGCCCGCTTTCACCATCGCAatc > 1:3144318/1‑69 (MQ=255)tctTCAATGATGCCGGAATCGACCATGGTTTCGAACGCCAGTTCAACGCCCGCTTTCACCATCGCAat > 1:3181218/1‑68 (MQ=255) atgatgCCGGAATCGAGCATGGTTTCGAACGCCAGTTCAACGCCCGCTTTCACCATCGCAatcatc < 1:2956986/66‑1 (MQ=255) atgatgCCGGAATCGACCATGGTTTCGAACGCCAGTTCAACGCCCGCTTTCACCATCGCAatcatc < 1:1423978/66‑1 (MQ=255) atgatgCCGGAATCGACCATGGTTTCGAACGCCAGTTCAACGCCCGCTTTCACCATCGCAatcatc < 1:847354/66‑1 (MQ=255) atgatgCCGGAATCGACCATGGTTTCGAACGCCAGTTCAACGCCCGCTTTCACCATCGCAatcatc < 1:1546740/66‑1 (MQ=255) atgatgCCGGAATCGACCATGGTTTCGAACGCCAGTTCAACGCCCGCTTTCACCATCGCAatcatc < 1:1325765/66‑1 (MQ=255) atgatgCCGGAATCGACCATGGTTTCGAACGCCAGTTCAACGCCCGCTTTCACCATCGCAatcatc < 1:3168586/66‑1 (MQ=255) atgatgCCGGAATCGACCATGGTTTCGAACGCCAGTTCAACGCCCGCTTTCACCATCGCAatcatc < 1:1350197/66‑1 (MQ=255) atgatgCCGGAATCGACCATGGTTTCGAACGCCAGTTCAACGCCCGCTTTCACCATCGCAatcatc < 1:752662/66‑1 (MQ=255) atgatgCCGGAATCGACCATGGTTTCGAACGCCAGTTCAACGCCCGCTTTCACCATCGCAatcatc < 1:1773915/66‑1 (MQ=255) atgatgCCGGAATCGACCATGGTTTCGAACGCCAGTTCAACGCCCGCTTTCACCATCGCAatcatc < 1:2767484/66‑1 (MQ=255) atgatgCCGGAATCGACCATGGTTTCGAACGCCAGTTCAACGCCCGCTTTCACCATCGCAatcatc < 1:274342/66‑1 (MQ=255) atgatgCCGGAATCGACCATGGTTTCGAACGCCAGTTCAACGCCCGCTTTCACCATCGCAatcatc < 1:751958/66‑1 (MQ=255) atgatgCCGGAATCGACCATGGTTTCGAACGCCAGTTCAACGCCCGCTTTCACCATCGCAATCATCAg < 1:3185525/68‑1 (MQ=255) atgatgCCGGAATCGACCATGGTTTCGAACGCCAGTTCAACGCCCGCTTTCACCATCGCAATCATCAg < 1:747094/68‑1 (MQ=255) tgatgCCGGAATCGACCATGGTTTCGAACGCCAGTTCAACGCCCGCTTTCACCATCGCAATCATCAGTa < 1:1643091/69‑1 (MQ=255) gatgCCGGAATCGACCATGGTTTCGAACGCCAGTTCAACGCCCGCTTTCACCATCGCAATCATCAGTa < 1:1973441/68‑1 (MQ=255) gatgCCGGAATCAACCATGGTTTCAAACGCCAACTCAACGCCTGCTTTTACCATCGCGATCATCAGTAc > 1:1811497/1‑69 (MQ=255) gatgCCGGAATCAACCATGGTTTCAAACGCCAACTCAACGCCTGCTTTTACCATCGCGATCATCAGTAc > 1:1422337/1‑69 (MQ=255) gatgCCGGAATCAACCATGGTTTCAAACGCCAACTCAACGCCTGCTTTTACCATCGCGATCATCAGTAc > 1:822403/1‑69 (MQ=255) gatgCCGGAATCAACCATGGTTTCAAACGCCAACTCAACGCCTGCTTTTACCATCGCGATCATCAGTAc > 1:1557789/1‑69 (MQ=255) gatgCCGGAATCAACCATGGTTTCAAACGCCAACTCAACGCCTGCTTTTACCATCGCGATCATCAGTAc > 1:2987486/1‑69 (MQ=255) gatgCCGGAATCAACCATGGTTTCAAACGCCAACTCAACGCCTGCTTTTACCATCGCGATCATCAGTAc > 1:1321545/1‑69 (MQ=255) gatgCCGGAATCAACCATGGTTTCAAACGCCAACTCAACGCCTGCTTTTACCATCGCGATCATCAGTAc > 1:3256387/1‑69 (MQ=255) gatgCCGGAATCAACCATGGTTTCAAACGCCAACTCAACGCCTGCTTTTACCATCGCGATCATCAGTAc > 1:447391/1‑69 (MQ=255) gatgCCGGAATCAACCATGGTTTCAAACGCCAACTCAACGCCTGCTTTTACCATCGCGATCATCAGTAc > 1:567570/1‑69 (MQ=255) gatgCCGGAATCAACCATGGTTTCAAACGCCAACTCAACGCCTGCTTTTACCATCGCGATCATCAGTAc > 1:1149744/1‑69 (MQ=255) gatgCCGGAATCAACCATGGTTTCAAACGCCAACTCAACGCCTGCTTTTACCATCGCGATCATCAGTAc > 1:595857/1‑69 (MQ=255) gatgCCGGAATCAACCATGGTTTCAAACGCCAACTCAACGCCTGCTTTTACCATCGCGATCATCAGTAc > 1:604949/1‑69 (MQ=255) gatgCCGGAATCAACCATGGTTTCAAACGCCAACTCAACGCCTGCTTTTACCATCGCGATCATCAGTAc > 1:2037425/1‑69 (MQ=255) gatgCCGGAATCAACCATGGTTTCAAACGCCAACTCAACGCCTGCTTTTACCATCGCGATCATCAGTAc > 1:2761832/1‑69 (MQ=255) gatgCCGGAATCAACCATGGTTTCAAACGCCAACTCAACGCCTGCTTTTACCATCGCGATCATCAGTAc > 1:1882685/1‑69 (MQ=255) gatgCCGGAATCAACCATGGTTTCAAACGCCAACTCAACGCCTGCTTTTACCATCGCGATCATCAGTAc > 1:2582695/1‑69 (MQ=255) gatgCCGGAATCAACCATGGTTTCAAACGCCAACTCAACGCCTGCTTTTACCATCGCGATCATCAGTAc > 1:2571512/1‑69 (MQ=255) gatgCCGGAATCAACCATGGTTTCAAACGCCAACTCAACGCCTGCTTTTACCATCGCGATCATCAGTAc > 1:246220/1‑69 (MQ=255) gatgCCGGAATCAACCATGGTTTCAAACGCCAACTCAACGCCTGCTTTTACCATCGCGATCATCAGTAc > 1:950326/1‑69 (MQ=255) gatgCCGGAATCAACCATGGTTTCAAACGCCAACTCAACGCCTGCTTTTACCATCGCGATCATCAGTAc > 1:64500/1‑69 (MQ=255) gatgCCGGAATCAACCATGGTTTCAAACGCCAACTCAACGCCTGCTTTTACCATCGCGATCATCAGTAc > 1:662912/1‑69 (MQ=255) gatgCCGGAATCAACCATGGTTTCAAACGCCAACTCAACGCCTGCTTTTACCATCGCGATCATCAGTAc > 1:722120/1‑69 (MQ=255) gatgCCGGAATCAACCATGGTTTCAAACGCCAACTCAACGCCTGCTTTTACCATCGCGATCATCAGTAc > 1:21591/1‑69 (MQ=255) gatgCCGGAATCAACCATGGTTTCAAACGCCAACTCAACGCCTGCTTTTACCATCGCGATCATCAGTAc > 1:2143389/1‑69 (MQ=255) gatgCCGGAATCAACCATGGTTTCAAACGCCAACTCAACGCCTGCTTTTACCATCGCGATCATCAGTAc > 1:885345/1‑69 (MQ=255) gatgCCGGAATCAACAATGGTTTCAAACGCCAACTCAACGCCTGCTTTTACCATCGCGATCATCAGTAc > 1:3226208/1‑69 (MQ=255) ggAATCGACCATGGTTTCGAACGCCAGTTCAACGCCCGCTTTCACCATCGCAatcatc < 1:2855503/58‑1 (MQ=255) ccATGGTTTCGAACGCCAGTTCAACGCCCGCTTTCACCATCGCAATCATCAGTACGCCTTTATCGAAg < 1:2931062/68‑1 (MQ=255) ccATGGTTTCGAACGCCAGTTCAACGCCCGCTTTCACCATCGCAATCATCAGTACGCCTTTATCGAAg < 1:1268706/68‑1 (MQ=255) ccATGGTTTCGAACGCCAGTTCAACGCCCGCTTTCACCATCGCAATCATCAGTACGCCTTTATCGAAg < 1:451198/68‑1 (MQ=255) ccATGGTTTCGAACGCCAGTTCAACGCCCGCTTTCACCATCGCAATCATCAGTACGCCTTTATCGAAg < 1:63579/68‑1 (MQ=255) ccATGGTTTCGAACGCCAGTTCAACGCCCGCTTTCACCATCGCAATCATCAGTACGCCTTTATCGAAg < 1:2829540/68‑1 (MQ=255) gTTTCGAACGCCAGTTCAACGCCCGCTTTCACCATCGCAATCATCAGTACGCCTTTATCGAAGTACTcc > 1:3405036/1‑69 (MQ=255) gTTTCGAACGCCAGTTCAACGCCCGCTTTCACCATCGCAATCATCAGTACGCCTTTATCGAAGTACTcc > 1:354062/1‑69 (MQ=255) ttCGACCGCCAGTTCAACGCCCGCTTTCACCATCGCAATCCTCAGTACGCCTTTATCGAAGTACTCCTg > 1:3101100/1‑69 (MQ=255) tCGAACGCCAGTTCAACGCCCGCTTTCACCATCGCAATCAT‑AGTACGCCTTTATCGAAGTACTCCTGCt > 1:454727/1‑69 (MQ=255) cGCCAGTTCAACGCCCGCTTTCACCATCGCAATCATCAGTa < 1:531034/41‑1 (MQ=255) cccGCTTTCACCATCGCAATCATCAGTACGCCTTTATCGAAGTACTCCTGCTCGCCGATTTTGCCTTCa < 1:2303700/69‑1 (MQ=255) cccGCTTTCACCATCGCAATCATCAGTACGCCTTTATCGAAGTACTCCTGCTCGCCGATTTTGCCTTCa < 1:1714603/69‑1 (MQ=255) ctgCTTTTACCATCGCGATCATCAGTACGCCTTTATCGAAGTACTCCTGTTCACCGATTTTGCCTTCAt < 1:1973008/67‑1 (MQ=255) ctgCTTTTACCATCGCGATCATCAGTACGCCTTTATCGAAGTACTCCTGTTCACCGATTTTGCCTTCAt < 1:972887/67‑1 (MQ=255) ctgCTTTTACCATCGCGATCATCAGTACGCCTTTATCGAAGTACTCCTGTTCACCGATTTTGCCTTCAt < 1:3372345/67‑1 (MQ=255) ctgCTTTTACCATCGCGATCATCAGTACGCCTTTATCGAAGTACTCCTGTTCACCGATTTTGCCTTCAt < 1:861423/67‑1 (MQ=255) ctgCTTTTACCATCGCGATCATCAGTACGCCTTTATCGAAGTACTCCTGTTCACCGATTTTGCCTTCAt < 1:1449830/67‑1 (MQ=255) ctgCTTTTACCATCGCGATCATCAGTACGCCTTTATCGAAGTACTCCTGTTCACCGATTTTGCCTTCAt < 1:1507216/67‑1 (MQ=255) ctgCTTTTACCATCGCGATCATCAGTACGCCTTTATCGAAGTACTCCTGTTCACCGATTTTGCCTTCAt < 1:1556060/67‑1 (MQ=255) ctgCTTTTACCATCGCGATCATCAGTACGCCTTTATCGAAGTACTCCTGTTCACCGATTTTGCCTTCAt < 1:430240/67‑1 (MQ=255) ctgCTTTTACCATCGCGATCATCAGTACGCCTTTATCGAAGTACTCCTGTTCACCGATTTTGCCTTCAt < 1:751126/67‑1 (MQ=255) ctgCTTTTACCATCGCGATCATCAGTACGCCTTTATCGAAGTACTCCTGTTCACCGATTTTGCCTTCAt < 1:2132531/67‑1 (MQ=255) ctgCTTTTACCATCGCGATCATCAGTACGCCTTTATCGAAGTACTCCTGTTCACCGATTTTGCCTTCAt < 1:2908527/67‑1 (MQ=255) ctgCTTTTACCATCGCGATCATCAGTACGCCTTTATCGAAGTACTCCTGTTCACCGATTTTGCCTTCAt < 1:2349725/67‑1 (MQ=255) ctgCTTTTACCATCGCGATCATCAGTACGCCTTTATCGAAGTACTCCTGTTCACCGATTTTGCCTTCAt < 1:2884817/67‑1 (MQ=255) ccGCTTTCACCATCGCAATCATCAGTACGCCTTTATCGAAGTACTCCTGCTCGCCGATTTTGCCTTCAt < 1:2205888/69‑1 (MQ=255) ccGCTTTCACCATCGCAATCATCAGTACGCCTTTATCGAAGTACTCCTGCTCGCCGATTTTGCCTTCAt < 1:857752/69‑1 (MQ=255) tgCTTTTACCATCGCGATCATCAGTACGCCTTTATCGAAGTACTCCTGTTCACCGATTTTGCCTTCAt < 1:2055025/67‑1 (MQ=255) aTCGCGATCATCAGTACGCCTTTATCGAAGTACTCCTGTTCACCGATTTTGCCTTCATACTGCGGcgcg < 1:2823917/69‑1 (MQ=255) aTCGCGATCATCAGTACGCCTTTATCGAAGTACTCCTGTTCACCGATTTTGCCTTCATACTGCGGcgcg < 1:729987/69‑1 (MQ=255) aTCGCGATCATCAGTACGCCTTTATCGAAGTACTCCTGTTCACCGATTTTGCCTTCATACTGCGGcgcg < 1:1271245/69‑1 (MQ=255) | TCTTCAATGATGCCGGAATCGACCATGGTTTCGAACGCCAGTTCAACGCCCGCTTTCACCATCGCAATCATCAGTACGCCTTTATCGAAGTACTCCTGCTCGCCGATTTTGCCTTCATACTGCGGCGCG > minE/2439493‑2439621 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |