| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,439,594 | G→A | 73.4% | G350G (GGC→GGT) | ilvC ← | ketol‑acid reductoisomerase, NAD(P)‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,439,594 | 0 | G | A | 73.4% | 97.1 / 35.6 | 90 | G350G (GGC→GGT) | ilvC | ketol‑acid reductoisomerase, NAD(P)‑binding |
| Reads supporting (aligned to +/- strand): ref base G (11/13); new base A (25/41); total (36/54) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.27e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCCGCTTTCACCATCGCAATCATCAGTACGCCTTTATCGAAGTACTCCTGCTCGCCGATTTTGCCTTCATACTGCGGCGCGGTTTCAAACGCGGTTTTGCCGGTCTCTTCACGCCAGGTC > minE/2439541‑2439660 | cccGCTTTCACCATCGCAATCATCAGTACGCCTTTATCGAAGTACTCCTGCTCGCCGATTTTGCCTTCa < 1:2303700/69‑1 (MQ=255)cccGCTTTCACCATCGCAATCATCAGTACGCCTTTATCGAAGTACTCCTGCTCGCCGATTTTGCCTTCa < 1:1714603/69‑1 (MQ=255) ctgCTTTTACCATCGCGATCATCAGTACGCCTTTATCGAAGTACTCCTGTTCACCGATTTTGCCTTCAt < 1:2132531/67‑1 (MQ=255) ctgCTTTTACCATCGCGATCATCAGTACGCCTTTATCGAAGTACTCCTGTTCACCGATTTTGCCTTCAt < 1:2349725/67‑1 (MQ=255) ctgCTTTTACCATCGCGATCATCAGTACGCCTTTATCGAAGTACTCCTGTTCACCGATTTTGCCTTCAt < 1:430240/67‑1 (MQ=255) ctgCTTTTACCATCGCGATCATCAGTACGCCTTTATCGAAGTACTCCTGTTCACCGATTTTGCCTTCAt < 1:972887/67‑1 (MQ=255) ctgCTTTTACCATCGCGATCATCAGTACGCCTTTATCGAAGTACTCCTGTTCACCGATTTTGCCTTCAt < 1:1973008/67‑1 (MQ=255) ctgCTTTTACCATCGCGATCATCAGTACGCCTTTATCGAAGTACTCCTGTTCACCGATTTTGCCTTCAt < 1:2908527/67‑1 (MQ=255) ctgCTTTTACCATCGCGATCATCAGTACGCCTTTATCGAAGTACTCCTGTTCACCGATTTTGCCTTCAt < 1:2884817/67‑1 (MQ=255) ctgCTTTTACCATCGCGATCATCAGTACGCCTTTATCGAAGTACTCCTGTTCACCGATTTTGCCTTCAt < 1:861423/67‑1 (MQ=255) ctgCTTTTACCATCGCGATCATCAGTACGCCTTTATCGAAGTACTCCTGTTCACCGATTTTGCCTTCAt < 1:1449830/67‑1 (MQ=255) ctgCTTTTACCATCGCGATCATCAGTACGCCTTTATCGAAGTACTCCTGTTCACCGATTTTGCCTTCAt < 1:751126/67‑1 (MQ=255) ctgCTTTTACCATCGCGATCATCAGTACGCCTTTATCGAAGTACTCCTGTTCACCGATTTTGCCTTCAt < 1:1507216/67‑1 (MQ=255) ctgCTTTTACCATCGCGATCATCAGTACGCCTTTATCGAAGTACTCCTGTTCACCGATTTTGCCTTCAt < 1:1556060/67‑1 (MQ=255) ctgCTTTTACCATCGCGATCATCAGTACGCCTTTATCGAAGTACTCCTGTTCACCGATTTTGCCTTCAt < 1:3372345/67‑1 (MQ=255) ccGCTTTCACCATCGCAATCATCAGTACGCCTTTATCGAAGTACTCCTGCTCGCCGATTTTGCCTTCAt < 1:2205888/69‑1 (MQ=255) ccGCTTTCACCATCGCAATCATCAGTACGCCTTTATCGAAGTACTCCTGCTCGCCGATTTTGCCTTCAt < 1:857752/69‑1 (MQ=255) tgCTTTTACCATCGCGATCATCAGTACGCCTTTATCGAAGTACTCCTGTTCACCGATTTTGCCTTCAt < 1:2055025/67‑1 (MQ=255) aTCGCGATCATCAGTACGCCTTTATCGAAGTACTCCTGTTCACCGATTTTGCCTTCATACTGCGGcgcg < 1:2823917/69‑1 (MQ=255) aTCGCGATCATCAGTACGCCTTTATCGAAGTACTCCTGTTCACCGATTTTGCCTTCATACTGCGGcgcg < 1:729987/69‑1 (MQ=255) aTCGCGATCATCAGTACGCCTTTATCGAAGTACTCCTGTTCACCGATTTTGCCTTCATACTGCGGcgcg < 1:1271245/69‑1 (MQ=255) catcaGTACGCCTTTATCGAAGTACTCCTGCTCGCCGATTTTGCCTTCATACTGCGGCGCGGTTTCaaa > 1:2022198/1‑69 (MQ=255) catcaGTACGCCTTTATCGAAGTACTCCTGCTCGCCGATTTTGCCTTCATACTGCGGCGCGGTTTCaaa > 1:3108301/1‑69 (MQ=255) catcaGTACGCCTTTATCGAAGTACTCCTGCTCGCCGATTTTGCCTTCATACTGCGGCGCGGTTTCaaa > 1:2738909/1‑69 (MQ=255) aGTACGCCTTTATCGAAGTACTCCTGTTCACCGATTTTGCCTTCATACTGCGTcgcgct > 1:1215614/1‑57 (MQ=255) aGTACGCCTTTATCGAAGTACTCCTGTTCACCGATTTTGCCTTCATACTGCGTCGCGGtt > 1:3363486/1‑60 (MQ=255) aGTACGCCTTTATCGAAGTACTCCTGTTCACCGATTTTGCCTTCATACTGCGTCGCGGtt > 1:407626/1‑60 (MQ=255) aGTACGCCTTTATCGAAGTACTCCTGTTCACCGATTTTGCCTTCATACTGCGTCGCGGtt > 1:2424372/1‑60 (MQ=255) aGTACGCCTTTATCGAAGTACTCCTGTTCACCGATTTTGCCTTCATACTGCGTCGCGGtt > 1:2420361/1‑60 (MQ=255) aGTACGCCTTTATCGAAGTACTCCTGTTCACCGATTTTGCCTTCATACTGCGTCGCGGtt > 1:2655557/1‑60 (MQ=255) aGTACGCCTTTATCGAAGTACTCCTGTTCACCGATTTTGCCTTCATACTGCGTCGCGGtt > 1:2941171/1‑60 (MQ=255) aGTACGCCTTTATCGAAGTACTCCTGTTCACCGATTTTGCCTTCATACTGCGTCGCGGtt > 1:2723211/1‑60 (MQ=255) aGTACGCCTTTATCGAAGTACTCCTGTTCACCGATTTTGCCTTCATACTGCGTCGCGGtt > 1:2338262/1‑60 (MQ=255) aGTACGCCTTTATCGAAGTACTCCTGTTCACCGATTTTGCCTTCATACTGCGTCGCGGtt > 1:2831171/1‑60 (MQ=255) aGTACGCCTTTATCGAAGTACTCCTGTTCACCGATTTTGCCTTCATACTGCGTCGCGGtt > 1:1103788/1‑60 (MQ=255) aGTACGCCTTTATCGAAGTACTCCTGTTCACCGATTTTGCCTTCATACTGCGTCGCGGtt > 1:1058972/1‑60 (MQ=255) aGTACGCCTTTATCGAAGTACTCCTGTTCACCGATTTTGCCTTCATACTGCGTCGCGGtt > 1:1345210/1‑60 (MQ=255) aGTACGCCTTTATCGAAGTACTCCTGTTCACCGATTTTGCCTTCATACTGCGTCGCGGtt > 1:1501191/1‑60 (MQ=255) aGTACGCCTTTATCGAAGTACTCCTGTTCACCGATTTTGCCTTCATACTGCGTCGCGGtt > 1:705299/1‑60 (MQ=255) aGTACGCCTTTATCGAAGTACTCCTGTTCACCGATTTTGCCTTCATACTGCGGCGCGGtt > 1:446514/1‑60 (MQ=255) aGTACGCCTTTATCGAAGTACTCCTGTTCACCGATTTTGCCTTCATACTGCGGCGCGGtt > 1:3101044/1‑60 (MQ=255) aGTACGCCTTTATCGAAGTACTCCTGTTCACCGATTTTGCCTTCATACTGCGGCGCGGtt > 1:1332611/1‑60 (MQ=255) aGTACGCCTTTATCGAAGTACTCCTGTTCACCGATTTTGCCTTCATACTGCGGCGCGGtt > 1:917150/1‑60 (MQ=255) aGTACGCCTTTATCGAAGTACTCCTGTTCACCGATTTTGCCTTCATACTGCGGCGCGGtt > 1:2741129/1‑60 (MQ=255) aGTACGCCTTTATCGAAGTACTCCTGTTCACCGATTTTGCCTTCATACTGCGGCGCGGtt > 1:837838/1‑60 (MQ=255) aGTACGCCTTTATCGAAGTACTCCTGTTCACCGATTTTGCCTTCATACTGCGGCGCGGtt > 1:184368/1‑60 (MQ=255) aGTACGCCTTTATCGAAGTACTCCTGTTCACCGATTTTGCCTTCATACTGCGGCGCGGtt > 1:2550862/1‑60 (MQ=255) aGTACGCCTTTATCGAAGTACTCCTGTTCACCGATTTTGCCTTCATACTGCGGCGCGGtt > 1:2407302/1‑60 (MQ=255) aGTACGCCTTTATCGAAGTACTCCTGTTCACCGATTTTGCCTTCATACTGCGGCGCGGtt > 1:448255/1‑60 (MQ=255) cGCCTTTATCGAAGTACTCCTGCTCGCCGATTTTGCCTTCATACTGCGGCGCGGTTTCAAACGCGGttt > 1:2193150/1‑69 (MQ=255) ccTTTATCGAAGTACTCCTGCTCGCCGATTTTGCCTTCATACTGCGGCGCGGTTTCAAACGCGGtttt < 1:480427/68‑1 (MQ=255) ttATCGAAGTACTCCTGTTCACCGATTTTGCCTTCa < 1:609458/36‑1 (MQ=37) ttATCGAAGTACTCCTGTTCACCGATTTTGCCTTCa < 1:414092/36‑1 (MQ=37) ttATCGAAGTACTCCTGTTCACCGATTTTGCCTTCa < 1:843674/36‑1 (MQ=37) ttATCGAAGTACTCCTGTTCACCGATTTTGCCTTCa < 1:553616/36‑1 (MQ=37) ttATCGAAGTACTCCTGTTCACCGATTTTGCCTTCa < 1:847686/36‑1 (MQ=37) ttATCGAAGTACTCCTGTTCACCGATTTTGCCTTCa < 1:542605/36‑1 (MQ=37) ttATCGAAGTACTCCTGTTCACCGATTTTGCCTTCa < 1:528570/36‑1 (MQ=37) ttATCGAAGTACTCCTGTTCACCGATTTTGCCTTCa < 1:984077/36‑1 (MQ=37) ttATCGAAGTACTCCTGTTCACCGATTTTGCCTTCa < 1:3346002/36‑1 (MQ=37) ttATCGAAGTACTCCTGTTCACCGATTTTGCCTTCa < 1:3272026/36‑1 (MQ=37) ttATCGAAGTACTCCTGTTCACCGATTTTGCCTTCa < 1:2037832/36‑1 (MQ=37) ttATCGAAGTACTCCTGTTCACCGATTTTGCCTTCa < 1:1594587/36‑1 (MQ=37) ttATCGAAGTACTCCTGTTCACCGATTTTGCCTTCa < 1:159864/36‑1 (MQ=37) ttATCGAAGTACTCCTGTTCACCGATTTTGCCTTCa < 1:1740737/36‑1 (MQ=37) ttATCGAAGTACTCCTGTTCACCGATTTTGCCTTCa < 1:1576420/36‑1 (MQ=37) ttATCGAAGTACTCCTGTTCACCGATTTTGCCTTCa < 1:2028245/36‑1 (MQ=37) ttATCGAAGTACTCCTGTTCACCGATTTTGCCTTCa < 1:2099218/36‑1 (MQ=37) ttATCGAAGTACTCCTGTTCACCGATTTTGCCTTCa < 1:2118455/36‑1 (MQ=37) ttATCGAAGTACTCCTGTTCACCGATTTTGCCTTCa < 1:1321396/36‑1 (MQ=37) ttATCGAAGTACTCCTGTTCACCGATTTTGCCTTCa < 1:2625171/36‑1 (MQ=37) ttATCGAAGTACTCCTGTTCACCGATTTTGCCTTCa < 1:2732209/36‑1 (MQ=37) ttATCGAAGTACTCCTGTTCACCGATTTTGCCTTCa < 1:3150607/36‑1 (MQ=37) tATCTAAGTACTCCTGTTCACGGATTTTGCCTTCa < 1:756715/35‑1 (MQ=16) tATCGAAGTACTCCTGTTCACCGATTTTGCCTTCa < 1:959802/35‑1 (MQ=37) aGTACTCCTGCTCGCCGATTTTGCCTTCATACTGCGGCGCGGTTTCAAACGCGGTTTTGCCGGTCTCtt < 1:2040964/69‑1 (MQ=255) aGTACTCCTGCTCGCCGATTTTGCCTTCATACTGCGGCGCGGTTTCAAACGCGGTTTTGCCGGTCTCtt < 1:673286/69‑1 (MQ=255) aGTACTCCTGCTCGCCGATTTTGCCTTCATACTGCGGCGCGGTTTCAAACGCGGTTTTGCCGGTCTCtt < 1:3150380/69‑1 (MQ=255) aGTACTCCTGCTCGCCGATTTTGCCTTCATACTGCGGCGCGGTTTCAAACGCAGTTTTGCCGGTCTCtt < 1:3366375/69‑1 (MQ=255) aGTACTCCTGCTCGCAGATTTTGCCTTCATACTGCGGCGCGGTTTCAAACGCGGTTTTGCCGGTCTCtt < 1:1381634/69‑1 (MQ=255) gTACTCCTGCTCGCCGATTTTGCCTTCATACTGCGGCGCGGTTTCAAACGCGGTTTTGCCGGTCTCTTc > 1:14385/1‑69 (MQ=255) gTACTCCTGCTCGCCGATTTTGCCTTCATACGGCGGCGCGGTTTCAAACGCGGTTTTGCCGGTCTCTTc > 1:18252/1‑69 (MQ=255) cTGCTCGCCGATTTTGCCTTCATACTGCGGCGCGGtt > 1:2032181/1‑37 (MQ=255) cTGCTCGCCGATTTTGCCTTCATACTGCGGCGCGGTTTCAAACGCGGTTTTGCCGGTCTCTTCACGCCa > 1:1251794/1‑69 (MQ=255) cTGCTCGCCGATTTTGCCTTCATACTGCGGCGCGGTTTCAAACGCGGTTTTGCCGGTCTCTTCACGCCa > 1:1867630/1‑69 (MQ=255) cTGCTCGCCGATTTTGCCTTCATACTGCGGCGCGGTTTCAAACGCGGTTTTGCCGGTCTCTTCACGCCa > 1:2361823/1‑69 (MQ=255) cTGCTCGCCGATTTTGCCTTCATACTGCGGCGCGGTTTCAAACGCGGTTTTGCCGGTCTCTTCAAGCCa > 1:1761087/1‑69 (MQ=255) cGCCGATTTTGCCTTCATACTGCGGCGCGGTTTCAAACGCGGTTTTGCCGGTCTCTTCACGCCAGGTc < 1:1764161/68‑1 (MQ=255) cGCCGATTTTGCCTTCATACTGCGGCGCGGTTTCAAACGCGGTTTTGCCGGTCTCTTCACGCCAGGTc < 1:243076/68‑1 (MQ=255) cGCCGATTTTGCCTTCATACTGCGGCGCGGTTTCAAACGCGGTTTTGCCGGTCTCTTCACGCCAGGTc < 1:2928032/68‑1 (MQ=255) | CCCGCTTTCACCATCGCAATCATCAGTACGCCTTTATCGAAGTACTCCTGCTCGCCGATTTTGCCTTCATACTGCGGCGCGGTTTCAAACGCGGTTTTGCCGGTCTCTTCACGCCAGGTC > minE/2439541‑2439660 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |