Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,439,852 T→C 18.4% A264A (GCA→GCG ilvC ← ketol‑acid reductoisomerase, NAD(P)‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,439,8520TC18.4% 190.3 / 29.8 124A264A (GCA→GCGilvCketol‑acid reductoisomerase, NAD(P)‑binding
Reads supporting (aligned to +/- strand):  ref base T (41/60);  new base C (8/15);  total (49/75)
Fisher's exact test for biased strand distribution p-value = 6.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.12e-01

Only 100 of 125 total aligned reads displayed.

AGCACGCAGTTTCGCCGGGTTAGAGAGACGGTCCATCATCAGGGTGATGCCGCCCTGTTTCAGTGCTTCGGTGATGGTTTCCCAACCGAACTGAATCAGTTTTTCTGCGTATGCTGGATCGGTACCTTC  >  minE/2439789‑2439917
                                                               |                                                                 
aGCACGCAGTTTCGCCGGGTTAGAGAGACGGTCCATCATCAGGGTGATGCCGCCCTGTTTCAGTGCtt                                                               >  1:730072/1‑68 (MQ=255)
aGCACGCAGTTTCGCCGGGTTAGAGAGACGGTCCATCATCAGGGTGATGCCGCCCTGTTTCAGTGCTTc                                                              >  1:1825106/1‑69 (MQ=255)
aGCACGCAGTTTCGCCGGGTTAGAGAGACGGTCCATCATCAGGGTGATGCCGCCCTGTTTCAGTGCTTc                                                              >  1:1733339/1‑69 (MQ=255)
aGCACGCAGTTTCGCCGGGTTAGAGAGACGGTCCATCATCAGGGTGATGCCGCCCTGTTTCAGTGCTTc                                                              >  1:2234843/1‑69 (MQ=255)
aGCACGCAGTTTCGCCGGGTTAGAGAGACGGTCCATCATCAGGGTGATGCCGCCCTGTTTCAGTGCTTc                                                              >  1:3182262/1‑69 (MQ=255)
aGCACGCAGTTTCGCCGGGTTAGAGAGACGGTCCATCATCAGGGTGATGCCGCCCTGTTTCAGTGCTTc                                                              >  1:538217/1‑69 (MQ=255)
   aCGCAGTTTCGCCGGGTTAGAGAGACGGTACATCATCAGGGTGATGCCGCCCTGTTTCAGTGCTTCGGt                                                           <  1:1280521/69‑1 (MQ=255)
          ttCGCCGGGTTAGAGAGACGGTGCATCATCAGGGTGATGCCGCCCTGTTTCAGTGCTTCGGTGATGGtt                                                    >  1:614201/1‑69 (MQ=255)
          ttCGCCGGGTTAGAGAGACGGTCCATCATCAGGGTGATGCCGCCCTGTTTCAGTGCTTCGGTGATGGtt                                                    >  1:229911/1‑69 (MQ=255)
          ttCGCCGGGTTAGAGAGACGGTCCATCATCAGGGTGATGCCGCCCTGTTTCAGTGCTTCGGTGATGGtt                                                    >  1:2/1‑69 (MQ=255)
          ttCGCCGGGTTAGAGAGACGGTCCATCATCAGGGTGATGCCGCCCTGTTTCAGTGCTTCGGTGATGGtt                                                    >  1:3353683/1‑69 (MQ=255)
          ttCGCCGGGTTAGAGAGACGGTCCATCATCAGGGTGATGCCGCCCTGTTTCAGTGCTTCGGTGATGGtt                                                    >  1:1541798/1‑69 (MQ=255)
          ttCGCCGGGTTAGAGAGACGGTCCATCATCAGGGTGATGCCGCCCTGTTTCAGTGCTTCGGTGATGGtt                                                    >  1:26339/1‑69 (MQ=255)
          ttCGCCGGGTTAGAGAGACGGTCCATCATCAGGGTGATGCCGCCCTGTTTCAGTGCTTCGGTGATGGtt                                                    >  1:1314095/1‑69 (MQ=255)
          ttCGCCGGGTTAGAGAGACGGTCCATCATCAGGGTGATGCCGCCCTGTTTCAGTGCTTCGGTGATGGtt                                                    >  1:878534/1‑69 (MQ=255)
           tCGCCGGGTTAGAGAGACGGTCCATCATCAGGGTGATGCCGCCCTGTTTCAGTGCTTCGGTGATGGttt                                                   >  1:615924/1‑69 (MQ=255)
           tCGCCGGGTTAGAGAGACGGTCCATCATCAGGGTGATGCCGCCCTGTTTCAGTGCTTCGGTGATGGttt                                                   >  1:1175437/1‑69 (MQ=255)
                   ttAGAGAGACGGTCCATCATCAGGGTGATGCCGCCCTGTTTCAGTGCTTCGGTGATGGTTTCCCAACCg                                           >  1:425281/1‑69 (MQ=255)
                   ttAGAGAGACGGTCCATCATCAGGGTGATGCCGCCCTGTTTCAGTGCTTCGGTGATGGTTTCCCAACCg                                           >  1:2955828/1‑69 (MQ=255)
                   ttAGAGAGACGGTCCATCATCAGGGTGATGCCGCCCTGTTTCAGTGCTTCGGTGATGGTTTCCCAACCg                                           >  1:3008766/1‑69 (MQ=255)
                        gagaCGGTCCATCATCAGGGTGATGCCGCCCTGTTTCAGTGCTTCggg                                                           <  1:621124/48‑2 (MQ=255)
                             ggTCCATCATCAGGGTGATGCCGCCCTGTTTCAGTGCTTCGGTGATGGTTTCCCAACCGAACTGAATCa                                 >  1:2432515/1‑69 (MQ=255)
                             ggTCCATCATCAGGGTGATGCCGCCCTGTTTCAGTGCTTCGGTGATGGTTTCCCAACCGAACTGAATCa                                 >  1:296401/1‑69 (MQ=255)
                             ggTCCATCATCAGGGTGATGCCGCCCTGTTTCAGTGCTTCGGTGATGGTTTCCCAACCGAACTGAATCa                                 >  1:3384082/1‑69 (MQ=255)
                             ggTCCATCATCAGGGTGATGCCGCCCTGTTTCAGTGCTTCGGTGATGGTTTCCCAACAGAACTGAATCa                                 >  1:3126747/1‑69 (MQ=255)
                             ggTCCATCATCAGGGTGATGCCGACCTGTTTCAGTGCTTCGGTGATGGTTTCCCAACCGAACTGAATCa                                 >  1:2527674/1‑69 (MQ=255)
                              gTCAATCATCAGGGTGATGCCGCCCTGTTTCAGTGCTTCGGTGATGGTTTCCCAACCGAACTGAATCAg                                <  1:808647/69‑1 (MQ=255)
                                 catcatCAGGGTGATGCCGCCCTGTTTCAGTGCTTCGGt                                                           <  1:309427/39‑1 (MQ=255)
                                 catcatCAGGGTGATGCCGCCCTGTTTCAGTGCTTCGGt                                                           <  1:1080195/39‑1 (MQ=255)
                                 catcatCAGGGTGATGCCGCCCTGTTTCAGTGCTTCGGt                                                           <  1:425718/39‑1 (MQ=255)
                                 catcatCAGGGTGATGCCGCCCTGTTTCAGTGCTTCGGt                                                           <  1:652064/39‑1 (MQ=255)
                                 catcatCAGGGTGATGCCGCCCTGTTTCAGTGCTTCGGt                                                           <  1:2338047/39‑1 (MQ=255)
                                 catcatCAGGGTGATGCCGCCCTGTTTCAGTGCTTCGGt                                                           <  1:97770/39‑1 (MQ=255)
                                 catcatCAGGGTGATGCCGCCCTGTTTCAGTGCTTCGGt                                                           <  1:22315/39‑1 (MQ=255)
                                 catcatCAGGGTGATGCCGCCCTGTTTCAGTGCTTCGGt                                                           <  1:1834220/39‑1 (MQ=255)
                                 catcatCAGGGTGATGCCGCCCTGTTTCAGTGCTTCGGt                                                           <  1:1238326/39‑1 (MQ=255)
                                 catcatCAGGGTGATGCCGCCCTGTTTCAGTGCTTCGGt                                                           <  1:2083102/39‑1 (MQ=255)
                                 catcatCAGGGTGATGACGCCCTGTTTCAGTGCTTCGGt                                                           <  1:361777/39‑1 (MQ=255)
                                   tcatcaGGGTGATGCCGCCCTGTTTCAGTGCTTCGGTGATGGTTTCCCAAGCGAACTGAATCAGtttt                            <  1:2060972/68‑1 (MQ=255)
                                   tcatcaGGGTGATGCCGCCCTGTTTCAGTGCTTCGGTGATGGTTTCCCAACCGAACTGAATCAGtttt                            <  1:3339800/68‑1 (MQ=255)
                                   tcatcaGGGTGATGCCGCCCTGTTTCAGTGCTTCGGTGATGGTTTCCCAACCGAACTGAATCAGtttt                            <  1:2724287/68‑1 (MQ=255)
                                   tcatcaGGGTGATGCCGCCCTGTTTCAGTGCTTCGGTGATGGTTTCCCAACCGAACTGAATCAGtttt                            <  1:3119871/68‑1 (MQ=255)
                                   tcatcaGGGTGATGCCGCCCTGTTTCAGTGCTTCGGTGATGGTTTCCCAACCGAACTGAATCAGtttt                            <  1:675288/68‑1 (MQ=255)
                                   tcatcaGGGTGATGCCGCCCTGTTTCAGTGCTTCGGTGATGGTTTCCCAACCGAACTGAATCAGtttt                            <  1:948514/68‑1 (MQ=255)
                                     atcaGGGTGATGCCGCCCTGTTTCAGTGCTTCGGTGATGGTTTCCCAACCGAACTGAATCAGtttt                            <  1:2519066/66‑1 (MQ=255)
                                     atcaGGGTGATGCCGCCCTGTTTCAGTGCTTCGGTGATGGTTTCCCAACCGAACTGAATCAGtttt                            <  1:754224/66‑1 (MQ=255)
                                     atcaGGGTGATGCCGCCCTGTTTCAGTGCTTCGGTGATGGTTTCCCAACCGAACTGAATCAGtttt                            <  1:343563/66‑1 (MQ=255)
                                     atcaGGGTGATGCCGCCCTGTTTCAGTGCTTCGGTGATGGTTTCCCAACCGAACTGAATCAGtttt                            <  1:278598/66‑1 (MQ=255)
                                     atcaGGGTGATGCCGCCCTGTTTCAGTGCTTCGGTGATGGTTTCCCAACCGAACTGAATCAGtttt                            <  1:1421562/66‑1 (MQ=255)
                                     atcaGGGTGATGCCGCCCTGTTTCAGTGCTTCGGTGATGGTTTCCCAACCGAACTGAATCAGtttt                            <  1:2820149/66‑1 (MQ=255)
                                     atcaGGGTGATGCCGCCCTGTTTCAGTGCTTCGGTGATGGTTTCCCAACCGAACTGAATCAGtttt                            <  1:529051/66‑1 (MQ=255)
                                     atcaGGGTGATGCCGCCCTGTTTCAGTGCTTCGGTGATGGTTTCCCAACCGAACTGAATCAGtttt                            <  1:3072051/66‑1 (MQ=255)
                                     atcaGGGTGATGCCGCCCTGTTTCAGTGCTTCGGTGATGGTTTCCCAACCGAACTGAATCAGtttt                            <  1:2020441/66‑1 (MQ=255)
                                     atcaGGGTGATGCCGCCCTGTTTCAGTGCTTCGGTGATGGTTTCCCAACCGAACTGAATCAGTTTTTCt                         >  1:1297463/1‑69 (MQ=255)
                                     atcaGGGTGATGCCGCCCTGTTTCAGTGCTTCGGTGATGGTTTCCCAACCGAACTGAATCAGTTTTTCt                         >  1:2917131/1‑69 (MQ=255)
                                        aGGGTGATGCCGCCCTGTTTCAGTGCTTCGGTGATGGTTTCCCAACCGAACTGAATCAGTTTTTCTGCg                      <  1:1678950/69‑1 (MQ=255)
                                        aGGGTGATGCCGCCCTGTTTCAGTGCTTCGGTGATGGTTTCCCAACCGAACTGAATCAGTTTTTCTGCg                      <  1:2937333/69‑1 (MQ=255)
                                        aGGGTGATGCCGCCCTGTTTCAGTGCTTCGGTGATGGTTTCCCAACCGAACTGAATCAGTTTTTCTGCg                      <  1:447677/69‑1 (MQ=255)
                                        aGGGTGATGCCGCCCTGTTTCAGTGCTTCGGTGATGGTTTCCCAACCGAACTGAATCAGTTTTTCTGCg                      <  1:2295124/69‑1 (MQ=255)
                                         gggTGATGCCGCCCTGTTTCAGTGCATCGGTGATGGTTTCCCAACCGAACTGAATCAGTTTTTCTGCg                      <  1:2586377/68‑1 (MQ=255)
                                          ggTGATGCCGCCCTGTTTCAGTGCTTCGGTGATGGTTTCCCAACCGAACTGAATCAGTTTTTCTGCGTa                    <  1:197455/69‑1 (MQ=255)
                                          ggTGATGCCGCCCTGTTTCAGTGCTTCGGTGATGGTTTCCCAACCGAACTGAATCAGTTTTTCTGCGTa                    <  1:2389288/69‑1 (MQ=255)
                                          ggTGATGCCGCCCTGTTTCAGTGCTTCGGAGATGGTTTCCCAACCGAACTGAATCAGTTTTTCTGCGTa                    <  1:2880217/69‑1 (MQ=255)
                                              aTGCCGCCCTGTTTCAGTGCTTCGGTGATGGTTTCCCAACCGAACTGAATCAGttt                             >  1:2368686/1‑56 (MQ=255)
                                                 ccgccCTGTTTCAGTGCTTCGGTGATGGTTTCCCAACCGAACTGAATCAGtttt                            <  1:56091/54‑1 (MQ=255)
                                                 ccgccCTGTTTCAGTGCTTCGGTGATGGTTTCCCAACCGAACTGAATCAGTTTTTCTGCGTATGCTGGa             >  1:2049960/1‑69 (MQ=255)
                                                 ccgccCTGTTTCAGTGCTTCGGTGATGGTTTCCCAACCGAACTGAATCAGTTTTTCTGCGTATGCTGGa             >  1:2815516/1‑69 (MQ=255)
                                                 ccgccCTGTTTCAGTGCTTCGGTGATGGTTTCCCAACCGAACTGAATCAGTTTTTCTGCGTATGCTGGa             >  1:1724285/1‑69 (MQ=255)
                                                   gccCTGTTTCAGTGCTTCGGTGATGGTTTCCCAACCGAACTGAATCAGTTTTTCTGCGTATGCTGGAt            <  1:1473406/68‑1 (MQ=255)
                                                   gccCTGTTTCAGTGCTTCGGTGATGGTTTCCCAACCGAACTGAATCAGTTTTTCTGCGTATGCTGGAt            <  1:1079438/68‑1 (MQ=255)
                                                   gccCTGTTTCAGTGCTTCGGTGATGGTTTCCCAACCGAACTGAATCAGTTTTTCTGCGTATGCTGGAt            <  1:2915247/68‑1 (MQ=255)
                                                   gccCTGTTTCAGTGCTTCGGTGATGGTTTCCCAACCGAACTGAATCAGTTTTTCTGCGTATGCTGGAt            <  1:2092296/68‑1 (MQ=255)
                                                   gccCTGTTTCAGTGCTTCGGTGATGGTTTCCCAACCGAACTGAATCAGTTTTTCTGCGTATACTGGAt            <  1:1898410/68‑1 (MQ=255)
                                                    cccTGTTTCAGCGCTTCGGTGATGGTTTCCCAACCGAACTGAATCAGTTTTTCTGCGTATGCCGGATCg          >  1:1278535/1‑69 (MQ=255)
                                                    cccTGTTTCAGCGCTTCGGTGATGGTTTCCCAACCGAACTGAATCAGTTTTTCTGCGTATGCCGGATCg          >  1:1298570/1‑69 (MQ=255)
                                                    cccTGTTTCAGCGCTTCGGTGATGGTTTCCCAACCGAACTGAATCAGTTTTTCTGCGTATGCCGGATCg          >  1:2530866/1‑69 (MQ=255)
                                                      cTGTTTCAGTGCTTCGGTGATGGTTTCCCAACCGAACTGAATCAGTTTTTCTGCGTATGCTGGATCGGt        <  1:2409342/69‑1 (MQ=255)
                                                      cTGTTTCAGTGCTTCGGTGATGGTTTCCCAACCGAACTGAATCAGTTTTTCTGCGTATGCTGGATCGGt        <  1:1308839/69‑1 (MQ=255)
                                                      cTGTTTCAGTGCTTCGGTGATGGTTTCCCAACCGAACTGAATCAGTTTTTCTGCGTATGCTGGATCGGt        <  1:138436/69‑1 (MQ=255)
                                                      cTGTTTCAGTGCTTCGGTGATGGTTTCCCAACCGAACTGAATCAGTTTTTCTGCGTATGCTGGATCGGt        <  1:2514619/69‑1 (MQ=255)
                                                      cTGTTTCAGTGCTTCGGTGATGGTTTCCCAACCGAACTGAATCAGTTTTTCTGCGTATGCTGGATCGGt        <  1:3154662/69‑1 (MQ=255)
                                                       tGTTTCAGCGCTTCGGTGATGGTTTCCCAACCGAACTGAATCAGTTTTTCTGCGTATGCCGGATCGGt        <  1:760880/68‑1 (MQ=255)
                                                       tGTTTCAGCGCTTCGGTGATGGTTTCCCAACCGAACTGAATCAGTTTTTCTGCGTATGCCGGATCGGt        <  1:1232043/68‑1 (MQ=255)
                                                       tGTTTCAGCGCTTCGGTGATGGTTTCCCAACCGAACTGAATCAGTTTTTCTGCGTATGCCGGATCGGt        <  1:839670/68‑1 (MQ=255)
                                                       tGTTTCAGCGCTTCGGTGATGGTTTCCCAACCGAACTGAATCAGTTTTTCTGCGTATGCCGGATCGGt        <  1:1091625/68‑1 (MQ=255)
                                                       tGTTTCAGCGCTTCGGTGATGGTTTCCCAACCGAACTGAATCAGTTTTTCTGCGTATGCCGGATCGGt        <  1:878800/68‑1 (MQ=255)
                                                       tGTTTCAGCGCTTCGGTGATGGTTTCCCAACCGAACTGAATCAGTTTTTCTGCGTATGCCGGATCGGt        <  1:655999/68‑1 (MQ=255)
                                                       tGTTTCAGCGCTTCGGTGATGGTTTCCCAACCGAACTGAATCAGTTTTTCTGCGTATGCCGGATCGGt        <  1:3428429/68‑1 (MQ=255)
                                                       tGTTTCAGCGCTTCGGTGATGGTTTCCCAACCGAACTGAATCAGTTTTTCTGCGTATGCCGGATCGGt        <  1:2635587/68‑1 (MQ=255)
                                                       tGTTTCAGCGCTTCGGTGATGGTTTCCCAACCGAACTGAATCAGTTTTTCTGCGTATGCCGGATCGGt        <  1:506530/68‑1 (MQ=255)
                                                       tGTTTCAGCGCTTCGGTGATGGTTTCCCAACCGAACTGAATCAGTTTTTCTGCGTATGCCGGATCGGt        <  1:454534/68‑1 (MQ=255)
                                                       tGTTTCAGCGCTTCGGTGATGGTTTCCCAACCGAACTGAATCAGTTTTTCTGCGTATGCCGGATCGGt        <  1:3160728/68‑1 (MQ=255)
                                                       tGTTTCAGCGCTTCGGTGATGGTTTCCCAACCGAACTGAATCAGTTTTTCTGCGTATGCCGGATCGGt        <  1:355928/68‑1 (MQ=255)
                                                       tGTTTCAGCGCTTCGGTGATGGTTTCCCAACCGAACTGAATCAGTTTTTCTGCGTATGCCGGATCGGt        <  1:3268554/68‑1 (MQ=255)
                                                       tGTTTCAGCGCTTCGGTGATGGTTTCCCAACCGAACTGAATCAGTTTTTCTGCGTATGCCGGATCGGt        <  1:1687580/68‑1 (MQ=255)
                                                        gTTTCAGTGCTTCGGTGATGGTTTCCCAACCGAACTGAATCAGTTTTTCTGCGTATGCTGGATCGGTAc      >  1:638253/1‑69 (MQ=255)
                                                         tttCAGTGCTTCGGTGATGGTTTCCCAACCGAACTGAATCAGtttt                            <  1:385498/46‑1 (MQ=255)
                                                           tCAGTGCTTCGGTGATGGTTTCCCAACCGAACTGAATCAGTTTTTCTGCGTATGCTGGATCGGTACct    <  1:2600827/68‑1 (MQ=255)
                                                             aGTGCTTCGGTGATGGTTTCCCAACCGAACTGAATCAGTTTTTCTGCGTATGCTGGATCGGTACcttc  <  1:2308419/68‑1 (MQ=255)
                                                               tGCTTCGGTGATGGTTTCCCAACCGAACTGAATCAGTTTTTCTGCGTATGCTGGATCGGt        <  1:2869690/60‑1 (MQ=255)
                                                               |                                                                 
AGCACGCAGTTTCGCCGGGTTAGAGAGACGGTCCATCATCAGGGTGATGCCGCCCTGTTTCAGTGCTTCGGTGATGGTTTCCCAACCGAACTGAATCAGTTTTTCTGCGTATGCTGGATCGGTACCTTC  >  minE/2439789‑2439917

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: