| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,439,903 | T→C | 25.4% | P247P (CCA→CCG) | ilvC ← | ketol‑acid reductoisomerase, NAD(P)‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,439,903 | 0 | T | C | 25.4% | 109.8 / 34.6 | 94 | P247P (CCA→CCG) | ilvC | ketol‑acid reductoisomerase, NAD(P)‑binding |
| Reads supporting (aligned to +/- strand): ref base T (37/33); new base C (8/16); total (45/49) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.55e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCGCCCTGTTTCAGTGCTTCGGTGATGGTTTCCCAACCGAACTGAATCAGTTTTTCTGCGTATGCTGGATCGGTACCTTCTTCCACCAGCTTGTCGAAGCACAGCAGAGAGCCAGCC > minE/2439838‑2439954 | ccgccCTGTTTCAGTGCTTCGGTGATGGTTTCCCAACCGAACTGAATCAGTTTTTCTGCGTATGCTgg > 1:2072169/1‑68 (MQ=255)ccgccCTGTTTCAGTGCTTCGGTGATGGTTTCCCAACCGAACTGAATCAGTTTTTCTGCGTATGCTGGa > 1:2049960/1‑69 (MQ=255)ccgccCTGTTTCAGTGCTTCGGTGATGGTTTCCCAACCGAACTGAATCAGTTTTTCTGCGTATGCTGGa > 1:2815516/1‑69 (MQ=255)ccgccCTGTTTCAGTGCTTCGGTGATGGTTTCCCAACCGAACTGAATCAGTTTTTCTGCGTATGCTGGa > 1:1724285/1‑69 (MQ=255) gccCTGTTTCAGTGCTTCGGTGATGGTTTCCCAACCGAACTGAATCAGTTTTTCTGCGTATGCTGGAt < 1:1079438/68‑1 (MQ=255) gccCTGTTTCAGTGCTTCGGTGATGGTTTCCCAACCGAACTGAATCAGTTTTTCTGCGTATGCTGGAt < 1:2092296/68‑1 (MQ=255) gccCTGTTTCAGTGCTTCGGTGATGGTTTCCCAACCGAACTGAATCAGTTTTTCTGCGTATGCTGGAt < 1:2915247/68‑1 (MQ=255) gccCTGTTTCAGTGCTTCGGTGATGGTTTCCCAACCGAACTGAATCAGTTTTTCTGCGTATGCTGGAt < 1:460108/68‑1 (MQ=255) gccCTGTTTCAGTGCTTCGGTGATGGTTTCCCAACCGAACTGAATCAGTTTTTCTGCGTATGCTGGAt < 1:1473406/68‑1 (MQ=255) gccCTGTTTCAGTGCTTCGGTGATGGTTTCCCAACCGAACTGAATCAGTTTTTCTGCGTATACTGGAt < 1:1898410/68‑1 (MQ=255) tccTGTTTCAGCGCTTCGGTGATGGTTTCCCAACCGAACTGAATCAGTTTTTCTGCGTATGCCGGATCg > 1:1351617/2‑69 (MQ=255) cccTGTTTCAGCGCTTCGGTGATGGTTTCCCAACCGAACTGAATCAGTTTTTCTGCGTATGCCGGATCg > 1:731974/1‑69 (MQ=255) cccTGTTTCAGCGCTTCGGTGATGGTTTCCCAACCGAACTGAATCAGTTTTTCTGCGTATGCCGGATCg > 1:2287509/1‑69 (MQ=255) cccTGTTTCAGCGCTTCGGTGATGGTTTCCCAACCGAACTGAATCAGTTTTTCTGCGTATGCCGGATCg > 1:1586482/1‑69 (MQ=255) cccTGTTTCAGCGCTTCGGTGATGGTTTCCCAACCGAACTGAATCAGTTTTTCTGCGTATGCCGGATCg > 1:2530866/1‑69 (MQ=255) cccTGTTTCAGCGCTTCGGTGATGGTTTCCCAACCGAACTGAATCAGTTTTTCTGCGTATGCCGGATCg > 1:1278535/1‑69 (MQ=255) cccTGTTTCAGCGCTTCGGTGATGGTTTCCCAACCGAACTGAATCAGTTTTTCTGCGTATGCCGGATCg > 1:2822624/1‑69 (MQ=255) cccTGTTTCAGCGCTTCGGTGATGGTTTCCCAACCGAACTGAATCAGTTTTTCTGCGTATGCCGGATCg > 1:1298570/1‑69 (MQ=255) cTGTTTCAGTGCTTCGGTGATGGTTTCCCAACCGAACTGAATCAGTTTTTCTGCGTATGCTGGATCGGt < 1:3154662/69‑1 (MQ=255) cTGTTTCAGTGCTTCGGTGATGGTTTCCCAACCGAACTGAATCAGTTTTTCTGCGTATGCTGGATCGGt < 1:2409342/69‑1 (MQ=255) cTGTTTCAGTGCTTCGGTGATGGTTTCCCAACCGAACTGAATCAGTTTTTCTGCGTATGCTGGATCGGt < 1:2514619/69‑1 (MQ=255) cTGTTTCAGTGCTTCGGTGATGGTTTCCCAACCGAACTGAATCAGTTTTTCTGCGTATGCTGGATCGGt < 1:138436/69‑1 (MQ=255) cTGTTTCAGTGCTTCGGTGATGGTTTCCCAACCGAACTGAATCAGTTTTTCTGCGTATGCTGGATCGGt < 1:1308839/69‑1 (MQ=255) tGTTTCAGCGCTTCGGTGATGGTTTCCCAACCGAACTGAATCAGTTTTTCTGCGTATGCCGGATCGGt < 1:3160728/68‑1 (MQ=255) tGTTTCAGCGCTTCGGTGATGGTTTCCCAACCGAACTGAATCAGTTTTTCTGCGTATGCCGGATCGGt < 1:3268554/68‑1 (MQ=255) tGTTTCAGCGCTTCGGTGATGGTTTCCCAACCGAACTGAATCAGTTTTTCTGCGTATGCCGGATCGGt < 1:760880/68‑1 (MQ=255) tGTTTCAGCGCTTCGGTGATGGTTTCCCAACCGAACTGAATCAGTTTTTCTGCGTATGCCGGATCGGt < 1:3428429/68‑1 (MQ=255) tGTTTCAGCGCTTCGGTGATGGTTTCCCAACCGAACTGAATCAGTTTTTCTGCGTATGCCGGATCGGt < 1:3430442/68‑1 (MQ=255) tGTTTCAGCGCTTCGGTGATGGTTTCCCAACCGAACTGAATCAGTTTTTCTGCGTATGCCGGATCGGt < 1:2635587/68‑1 (MQ=255) tGTTTCAGCGCTTCGGTGATGGTTTCCCAACCGAACTGAATCAGTTTTTCTGCGTATGCCGGATCGGt < 1:355928/68‑1 (MQ=255) tGTTTCAGCGCTTCGGTGATGGTTTCCCAACCGAACTGAATCAGTTTTTCTGCGTATGCCGGATCGGt < 1:454534/68‑1 (MQ=255) tGTTTCAGCGCTTCGGTGATGGTTTCCCAACCGAACTGAATCAGTTTTTCTGCGTATGCCGGATCGGt < 1:506530/68‑1 (MQ=255) tGTTTCAGCGCTTCGGTGATGGTTTCCCAACCGAACTGAATCAGTTTTTCTGCGTATGCCGGATCGGt < 1:655999/68‑1 (MQ=255) tGTTTCAGCGCTTCGGTGATGGTTTCCCAACCGAACTGAATCAGTTTTTCTGCGTATGCCGGATCGGt < 1:839670/68‑1 (MQ=255) tGTTTCAGCGCTTCGGTGATGGTTTCCCAACCGAACTGAATCAGTTTTTCTGCGTATGCCGGATCGGt < 1:878800/68‑1 (MQ=255) tGTTTCAGCGCTTCGGTGATGGTTTCCCAACCGAACTGAATCAGTTTTTCTGCGTATGCCGGATCGGt < 1:1091625/68‑1 (MQ=255) tGTTTCAGCGCTTCGGTGATGGTTTCCCAACCGAACTGAATCAGTTTTTCTGCGTATGCCGGATCGGt < 1:1687580/68‑1 (MQ=255) tGTTTCAGCGCTTCGGTGATGGTTTCCCAACCGAACTGAATCAGTTTTTCTGCGTATGCCGGATCGGt < 1:1232043/68‑1 (MQ=255) gTTTCAGTGCTTCGGTGATGGTTTCCCAACCGAACTGAATCAGTTTTTCTGCGTATGCTGGATCGGTAc > 1:638253/1‑69 (MQ=255) tCAGTGCTTCGGTGATGGTTTCCCAACCGAACTGAATCAGTTTTTCTGCGTATGCTGGATCGGTACct < 1:2600827/68‑1 (MQ=255) aGTGCTTCGGTGATGGTTTCCCAACCGAACTGAATCAGTTTTTCTGCGTATGCTGGATCGGTACcttc < 1:2308419/68‑1 (MQ=255) tGCTTCGGTGATGGTTTCCCAACCGAACTGAATCAGTTTTTCTGCGTATGCTGGATCGGt < 1:2869690/60‑1 (MQ=255) gTTTCCGAACCGAACTGAATCAGTTTTTCTGCGTATGCTGGATCGGTACCTTCTTCCACCAGCTTGt > 1:437465/1‑67 (MQ=255) gTTTCCCAACCGAACTGAATCAGTTTTTCTGCGTATGCTGGATCGGTACCttcttc > 1:905948/1‑56 (MQ=255) gTTTCCCAACCGAACTGAATCAGTTTTTCTGCGTATGCTGGATCGGTACCTTCTTCCACCAGCTTGt > 1:485682/1‑67 (MQ=255) gTTTCCCAACCGAACTGAATCAGTTTTTCTGCGTATGCTGGATCGGTACCTTCTTCCACCAGCTTGt > 1:474596/1‑67 (MQ=255) gTTTCCCAACCGAACTGAATCAGTTTTTCTGCGTATGCTGGATCGGTACCTTCTTCCACCAGCTTGt > 1:450431/1‑67 (MQ=255) gTTTCCCAACCGAACTGAATCAGTTTTTCTGCGTATGCTGGATCGGTACCTTCTTCCACCAGCTTGt > 1:222996/1‑67 (MQ=255) gTTTCCCAACCGAACTGAATCAGTTTTTCTGCGTATGCTGGATCGGTACCTTCTTCCACCAGCTTGt > 1:388188/1‑67 (MQ=255) gTTTCCCAACCGAACTGAATCAGTTTTTCTGCGTATGCTGGATCGGTACCTTCTTCCACCAGCTTGt > 1:829121/1‑67 (MQ=255) gTTTCCCAACCGAACTGAATCAGTTTTTCTGCGTATGCTGGATCGGTACCTTCTTCCACCAGCTTGt > 1:1625490/1‑67 (MQ=255) gTTTCCCAACCGAACTGAATCAGTTTTTCTGCGTATGCTGGATCGGTACCTTCTTCCACCAGCTTGt > 1:1677736/1‑67 (MQ=255) gTTTCCCAACCGAACTGAATCAGTTTTTCTGCGTATGCTGGATCGGTACCTTCTTCCACCAGCTTGt > 1:3394692/1‑67 (MQ=255) gTTTCCCAACCGAACTGAATCAGTTTTTCTGCGTATGCTGGATCGGTACCTTCTTCCACCAGCTTGt > 1:1111810/1‑67 (MQ=255) gTTTCCCAACCGAACTGAATCAGTTTTTCTGCGTATGCTGGATCGGTACCTTCTTCCACCAGCTTGt > 1:1688104/1‑67 (MQ=255) gTTTCCCAACCGAACTGAATCAGTTTTTCTGCGTATGCTGGATCGGTACCTTCTTCCACCAGCTTGt > 1:2918617/1‑67 (MQ=255) gTTTCCCAACCGAACTGAATCAGTTTTTCTGCGTATGCTGGATCGGTACCTTCTTCCACCAGCTTGt > 1:2913515/1‑67 (MQ=255) gTTTCCCAACCGAACTGAATCAGTTTTTCTGCGTATGCTGGATCGGTACCTTCTTCCACCAGCTTGt > 1:2101682/1‑67 (MQ=255) gTTTCCCAACCGAACTGAATCAGTTTTTCTGCGTATGCTGGATCGGTACCTTCTTCCACCAGCTTGt > 1:1749805/1‑67 (MQ=255) gTTTCCCAACCGAACTGAATCAGTTTTTCTGCGTATGCTGGATCGGTACCTTCTTCCACCAGCTTGt > 1:2767714/1‑67 (MQ=255) gTTTCCCAACCGAACTGAATCAGTTTTTCTGCGTATGCTGGATCGGTACCTTCTTCCACCAGCTTGt > 1:2762441/1‑67 (MQ=255) ttCCCAACCGAACTGAATCAGTTTTTCTGCGTATGCTGGa > 1:2506806/1‑40 (MQ=255) ttCCCAACCGAACTGAATCAGTTTTTCTGCGTATGCTGGATCGGTACcttc > 1:160617/1‑51 (MQ=255) ttCCCAACCGAACTGAATCAGTTTTTCTGCGTATGCTGGATCGGTACCTTCTTCCACCAGCTTGTCGa > 1:251794/1‑68 (MQ=255) ttCCCAACCGAACTGAATCAGTTTTTCTGCGTATGCTGGATCGGTACCTTCTTCCACCAGCTTGTCGa > 1:2333583/1‑68 (MQ=255) ttCCCAACCGAACTGAATCAGTTTTTCTGCGTATGCTGGATCGGTACCTTCTTCCACCAGCTTGTCGa > 1:12831/1‑68 (MQ=255) ttCCCAACCGAACTGAATCAGTTTTTCTGCGTATGCTGGATCGGTACCTTCTTCCACCAGCTTGTCGa > 1:1422321/1‑68 (MQ=255) ttCCCAACCGAACTGAATCAGTTTTTCTGCGTATGCTGGATCGGTACCTTCTTCCACCAGCTTGTCGa > 1:1528567/1‑68 (MQ=255) ttCCCAACCGAACTGAATCAGTTTTTCTGCGTATGCTGGATCGGTACCTTCTTCCACCAGCTTGTCGa > 1:1581703/1‑68 (MQ=255) ttCCCAACCGAACTGAATCAGTTTTTCTGCGTATGCTGGATCGGTACCTTCTTCCACCAGCTTGTCGa > 1:1833946/1‑68 (MQ=255) ttCCCAACCGAACTGAATCAGTTTTTCTGCGTATGCTGGATCGGTACCTTCTTCCACCAGCTTGTCGa > 1:1819214/1‑68 (MQ=255) ttCCCAACCGAACTGAATCAGTTTTTCTGCGTATGCTGGATCGGTACCTTCTTCCACCAGCTTGTCGa > 1:2851895/1‑68 (MQ=255) ttCCCAACCGAACTGAATCAGTTTTTCTGCGTATGCTGGATCGGTACCTTCTTCCACCAGCTTGTCGa > 1:2797660/1‑68 (MQ=255) ttCCCAACCGAACTGAATCAGTTTTTCTGCGTATGCTGGATCGGTACCTTCTTCCACCAGCTTGTCGa > 1:2787992/1‑68 (MQ=255) tCCCAACCGAACTGAATCAGTTTTTCTGCGTATGCTGGATCGGTACCTTCTTCCACCAGCTTGTCGAAg < 1:1915971/69‑1 (MQ=255) tCCCAACCGAACTGAATCAGTTTTTCTGCGTATGCTGGATCGGTACCTTCTTCCACCAGCTTGTCGAAg < 1:185527/69‑1 (MQ=255) tCCCAACCGAACTGAATCAGTTTTTCTGCGTATGCTGGATCGGTACCTTCTTCCACCAGCTTGTCGAAg < 1:1591746/69‑1 (MQ=255) tCCCAACCGAACTGAATCAGTTTTTCTGCGTATGCTGGATCGGTACCTTCTTCCACCAGCTTGTCGAAg < 1:2971222/69‑1 (MQ=255) tCCCAACCGAACTGAATCAGTTTTTCTGCGTATGCTGGATCGGTACCTTCTTCCACCAGCTTGTCGAAg < 1:671137/69‑1 (MQ=255) cAACCGAACTGAATCAGTTTTTCTGCGTATGCTGGATCGGTACCttcttc < 1:3061453/50‑1 (MQ=255) cAACCGAACTGAATCAGTTTTTCTGCGTATGCTGGATCGGTACCttcttc < 1:682178/50‑1 (MQ=255) cAACCGAACTGAATCAGTTTTTCTGCGTATGCCGGATCGGt < 1:2001144/41‑1 (MQ=255) cGAACTGAATCAGTTTTTCTGCGTATGCTGGATCGGTACCTTCTTCCACCAGCTTGTCGAAGCAcagca < 1:271318/69‑1 (MQ=255) cGAACTGAATCAGTTTTTCTGCGTATGCTGGATCGGTACCTTCTTCCACCAGCTTGTCGAAGCAcagca < 1:2495567/69‑1 (MQ=255) cGAACTGAATCAGTTTTTCTGCGTATGCTGGATCGGTACCTTCTTCCACCAGCTTGTCGAAGCAcagca < 1:1683379/69‑1 (MQ=255) cTGAATCAGTTTTTCTGCGTATGCTGGATCGGTAGct < 1:737276/37‑1 (MQ=255) tCAGTTTTTCTGCGTATGCTGGATCGGTACCTTCTTCCACCAGCTTGTCGAAGCACAGCAGAGagccag < 1:473144/69‑1 (MQ=255) tCAGTTTTTCTGCGTATGCTGGATCGGTACCTTCTTCCACCAGCTTGTCGAAGCACAGCAGAGagccag < 1:3130219/69‑1 (MQ=255) tCAGTTTTTCT‑‑GTATGCTGGATCGGTACCTTCTTCCACCAGCTTGTCGAAGCACAGCAGAGagccag < 1:3226652/67‑1 (MQ=255) cAGTTTTTCTGCGTATGCTGGATCGGTACCTTCTTCCACCAGCTTGTCGAAGCACAGCAGAGagccag < 1:1530731/68‑1 (MQ=255) cAGTTTTTCTGCGTATGCTGGATCGGTACCTTCTTCCACCAGCTTGTCGAAGCACAGCAGAGagccag < 1:1797022/68‑1 (MQ=255) aGTTTTTCTGCGTATGCTGGATCGGTACCTTCTTCCACCAGCTTGTCGAAGCACAGCAGAGagccagcc < 1:2497173/69‑1 (MQ=255) aGTTTTTCTGCGTATGCTGGATCGGTACCTTCTTCCACCAGCTTGTCGAAGCACAGCAGAGagccagcc < 1:2693083/69‑1 (MQ=255) ttCTGCGTATGCTGGATCGGTACCTTCTTCCACCAGCTTGTCGAAg < 1:1696221/46‑1 (MQ=255) | CCGCCCTGTTTCAGTGCTTCGGTGATGGTTTCCCAACCGAACTGAATCAGTTTTTCTGCGTATGCTGGATCGGTACCTTCTTCCACCAGCTTGTCGAAGCACAGCAGAGAGCCAGCC > minE/2439838‑2439954 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |