| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,440,065 | G→T | 48.3% | A193A (GCC→GCA) | ilvC ← | ketol‑acid reductoisomerase, NAD(P)‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,440,065 | 0 | G | T | 48.3% | 9.1 / 84.2 | 78 | A193A (GCC→GCA) | ilvC | ketol‑acid reductoisomerase, NAD(P)‑binding |
| Reads supporting (aligned to +/- strand): ref base G (14/26); new base T (20/17); total (35/43) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.12e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.48e-01 | |||||||||||
TTTCACTTCCGCAACGAAGGACGATTCCAGCACACCCGCACGGTGACCACCGGTTGCAGCCGCCCAGGCTTTGGCAATCGCCATGCCTTCGCCTTTCGGATCGTTTTCCGGGTGAACGGCAATCAGCGTCGG > minE/2439999‑2440130 | tttCACTTCCGCAACGAAGGACGATTCCAGCACACCCGCACGGTGACCACCGGTTGCAGCCGCCCAGGc > 1:91032/1‑69 (MQ=255)tttCACTTCCGCAACGAAGGACGATTCCAGCACACCCGCACGGTGACCACCGGTTGCAGCCGCCCAGGc > 1:3212241/1‑69 (MQ=255)tttCACTTCCGCAACGAAGGACGATTCCAGCACACCCGCACGGTGACCACCGGTTGCAGCCGCCCAGGc < 1:284641/69‑1 (MQ=255)tttCACTTCCGCAACGAAGGACGATTCCAGCACACCCGCACGGTGACCACCGGTTGCAGCCGCCCAGGc < 1:2108704/69‑1 (MQ=255)tttCACTTCCGCAACGAAGGACGATTCCAGCACACCCGCACGGTGACCACCGGTTGCAGCCGCCCAGGc > 1:2057751/1‑69 (MQ=255)tttCACTTCCGCAACGAAGGACGATTCCAGCACACCCGCACGGTGACCACCGGTTGCAGCCGCCCAGGc < 1:1592315/69‑1 (MQ=255)tttCACTTCCGCAACGAAGGACGATTCCAGCACACCCGCACGGTGACCACCGGTTGCAGCCGCCCAGGc < 1:142451/69‑1 (MQ=255)tttCACTTCCGCAACGAAGGACGATTCCAGCACACCCGCACGGTGACCACCGGTTGCAGCCGCCCAGGc > 1:755028/1‑69 (MQ=255) aaCGAAGGACGATTCCAGCACACCCGCACGGTGACCACCGGTTGCAGCCGCCCAGGCTTTGGCAATCGc < 1:3223228/69‑1 (MQ=255) aaCGAAGGACGATTCCAGCACACCCGCACGGTGACCACCGGTTGCAGCCGCCCAGGCTTTGGCAATCGc < 1:1354834/69‑1 (MQ=255) aaCGAAGGACGATTCCAGCACACCCGCACGGTGACCACCGGTTGCAGCCGCCCAGGCTTTGGCAATCGc < 1:2134827/69‑1 (MQ=255) aaCGAAGGACGATTCCAGCACACCCGCACGGTGACCACCGGTTGCAGCCGCCCAGGCTTTGGCAATCGc < 1:2363101/69‑1 (MQ=255) aaCGAAGGACGATTCCAGCACACCCGCACGGTGACCACCGGTTGCAGCCGCCCAGGCTTTGGCAATCGc < 1:2459756/69‑1 (MQ=255) aaCGAAGGACGATTCCAGCACACCCGCACGGTGACCACCGGTTGCAGCCGCCCAGGCTTTGGCAATCGc < 1:3279006/69‑1 (MQ=255) aaCGAAGGACGATTCCAGCACACCCGCACGGTGACCACCGGTTGCAGCCGCCCAGGCTTTGGCAATCGc < 1:500592/69‑1 (MQ=255) aaCGAAGGACGATTCCAGCACACCCGCACGGTGACCACCGGTTGCAGCCGCCCAGGCTTTGGCAATCGc < 1:1570741/69‑1 (MQ=255) aaCGAAGGACGATTCCAGCACACCCGCACGGTGACCACCGGTTGCAGCCGCCCAGGCTTTGGCAATCGc < 1:2908958/69‑1 (MQ=255) aaCGAAGGACGATTCCAGCACACCCGCACGGTGACCACCGGTTGCAGCCGCCCAGGCTTTGGCAATCGc < 1:624166/69‑1 (MQ=255) aaCGAAGGACGATTCCAGCACACCCGCACGGTGACCACCGGTTGCAGCCGCCCAGGCTTTGGCAATCGc < 1:1458692/69‑1 (MQ=255) aCGAAGGACGATTCCAGCACACCCGCACGGTGACCACCGGTTGCAGCCGCCCAGGCTTTGGCAATCGc < 1:2315423/68‑1 (MQ=255) gAAGGACGATTCCAGCACACCCGCACGGTGACCACCGGTTGCAGCCGCCCAGGCTTTGGCAATCGCCAt < 1:764380/69‑1 (MQ=255) gAAGGACGATTCCAGCACACCCGCACGGTGACCACCGGTTGCAGCCGCCCAGGCTTTGGCAATCGCCAt < 1:2359557/69‑1 (MQ=255) gAAGGACGATTCCAGCACACCCGCACGGTGACCACCGGTTGCAGCCGCCCAGGCTTTGGCAATCGCCAt < 1:863405/69‑1 (MQ=255) cacaCCCGCACGGTGACCACCGGTTGCAGCCGCCCAGGCTTTGGCAATCGc < 1:347918/51‑1 (MQ=255) cccGCACGGTGACCACCGGTTGCAGCCGCCCAGGCTTTGGCAATcac > 1:1052004/1‑45 (MQ=255) cccGCACGGTGACCACCGGTTGCAGCCGCCCAGGCTTTGGCAATCGc > 1:1240751/1‑47 (MQ=255) cccGCACGGTGACCACCGGTTGCAGCCGCCCAGGCTTTGGCAATCGc > 1:1605963/1‑47 (MQ=255) gTGACCACCGGTTGCAGCCGCCCAGGCTTTGGCAATCGCCATGCCTTCGCCTTTCGGATCGTTTTCCgg > 1:365460/1‑69 (MQ=255) gTGACCACCGGTTGCAGCCGCCCAGGCTTTGGCAATCGCCATGCCTTCGCCTTTCGGATCGTTTTCCgg > 1:694804/1‑69 (MQ=255) gTGACCACCGGTTGCAGCCGCCCAGGCTTTGGCAATCGCCATGCCTTCGCCTTTCGGATCGTTTTCCgg > 1:2085448/1‑69 (MQ=255) gTGACCACCGGTTGCAGCCGCCCAGGCTTTGGCAATCGCCATGCCTTCGCCTTTCGGATCGTTTTCCgg > 1:3432359/1‑69 (MQ=255) accaccGGTTGCAGCCGCCCAGGCTTTGGCAATCGCCATGCCTTCGCCTTTCGGATCGTTTTCCGGGTg < 1:2298248/69‑1 (MQ=255) accaccGGTTGCAGCCGCCCAGGCTTTGGCAATCGCCATGCCTTCGCCTTTCGGATCGTTTTCCGGGTg < 1:2903982/69‑1 (MQ=255) accaccGGTTGCAGCCGCCCAGGCTTTGGCAATCGCCATGCCTTCGCCTTTCGGATCGTTTTCCGGGTg < 1:366580/69‑1 (MQ=255) tGCAGCCGCCCATGCTTTAGCGATCGCCATGCCTTCGCCTTTCGTATCGTTTTcc < 1:217738/55‑1 (MQ=255) tGCAGCCGCCCATGCTTTAGCGATCGCCATGCCTTCGCCTTTCGGATCGTTTTcc < 1:3361443/55‑1 (MQ=255) tGCAGCCGCCCATGCTTTAGCGATCGCCATGCCTTCGCCTTTCGGATCGTTTTcc < 1:3168866/55‑1 (MQ=255) tGCAGCCGCCCATGCTTTAGCGATCGCCATGCCTTCGCCTTTCGGATCGTTTTcc < 1:3036141/55‑1 (MQ=255) tGCAGCCGCCCATGCTTTAGCGATCGCCATGCCTTCGCCTTTCGGATCGTTTTcc < 1:2915635/55‑1 (MQ=255) tGCAGCCGCCCATGCTTTAGCGATCGCCATGCCTTCGCCTTTCGGATCGTTTTcc < 1:167913/55‑1 (MQ=255) tGCAGCCGCCCATGCTTTAGCGATCGCCATGCCTTCGCCTTTCGGATCGTTTTcc < 1:1747582/55‑1 (MQ=255) tGCAGCCGCCCATGCTTTAGCGATCGCCATGCCTTCGCCTTTCGGATCGTTTTcc < 1:2790277/55‑1 (MQ=255) tGCAGCCGCCCATGCTTTAGCGATCGCCATGCCTTCGCCTTTCGGATCGTTTTcc < 1:273202/55‑1 (MQ=255) tGCAGCCGCCCATGCTTTAGCGATCGCCATGCCTTCGCCTTTCGGATCGTTTTcc < 1:2588084/55‑1 (MQ=255) tGCAGCCGCCCATGCTTTAGCGATCGCCATGCCTTCGCCTTTCGGATCGTTTTcc < 1:2556854/55‑1 (MQ=255) tGCAGCCGCCCATGCTTTAGCGATCGCCATGCCTTCGCCTTTCGGATCGTTTTcc < 1:1025155/55‑1 (MQ=255) tGCAGCCGCCCATGCTTTAGCGATCGCCATGCCTTCGCCTTTCGGATCGTTTTcc < 1:1922125/55‑1 (MQ=255) tGCAGCCGCCCATGCTTTAGCGATCGCCATGCCTTCGCCTTTCGGATCGTTTTcc < 1:2446943/55‑1 (MQ=255) tGCAGCCGCCCATGCTTTAGCGATCGCCATGCCTTCGCCTTTCGGATCGTTTTcc < 1:2083932/55‑1 (MQ=255) tGCAGCCGCCCATGCTTTAGCGATCGCCATGCCTTCGCCTTTCGGATCGTTTTcc < 1:1104670/55‑1 (MQ=255) tGCAGCCGCCCATGCTTTAGCGATCGCCATGCCTTCGCCTTTCGGATCGTTTTcc < 1:2319254/55‑1 (MQ=255) tGCAGCCGCCCAGGCTTTGGCAATCGCCATGCCTTCGCCTTTCGGATCGTTTTCCGGGTGAACGGCa > 1:2590146/1‑67 (MQ=255) tGCAGCCGCCCAGGCTTTGGCAATCGCCATGCCTTCGCCTTTCGGATCGTTTTCCGGGTGAACGGCa > 1:2552856/1‑67 (MQ=255) tGCAGCCGCCCAGGCTTTGGCAATCGCCATGCCTTCGCCTTTCGGATCGTTTTCCGGGTGAACGGCa > 1:1262030/1‑67 (MQ=255) ccgccCATGCTTTAGCTATCGCCATGCCTTCGCCTTTCGGATCGTTTTCCGGGTGAACGGCAATCAGCg > 1:1890051/1‑69 (MQ=255) ccgccCATGCTTTAGCGCTCGCCATGCCTTCGCCTTTAGGATCGTTTTCCGGGTGAACGGCAATCAGCg > 1:250339/1‑69 (MQ=255) ccgccCATGCTTTAGCGATCGCCATGCCTTCGCCTTTCGGATCGTTTTCCGGGTGAACGGCa > 1:1645262/1‑62 (MQ=255) ccgccCATGCTTTAGCGATCGCCATGCCTTCGCCTTTCGGATCGTTTTCCGGGTGAACGGCAATCAGCg > 1:2806572/1‑69 (MQ=255) ccgccCATGCTTTAGCGATCGCCATGCCTTCGCCTTTCGGATCGTTTTCCGGGTGAACGGCAATCAGCg > 1:1047305/1‑69 (MQ=255) ccgccCATGCTTTAGCGATCGCCATGCCTTCGCCTTTCGGATCGTTTTCCGGGTGAACGGCAATCAGCg > 1:909501/1‑69 (MQ=255) ccgccCATGCTTTAGCGATCGCCATGCCTTCGCCTTTCGGATCGTTTTCCGGGTGAACGGCAATCAGCg > 1:874156/1‑69 (MQ=255) ccgccCATGCTTTAGCGATCGCCATGCCTTCGCCTTTCGGATCGTTTTCCGGGTGAACGGCAATCAGCg > 1:1251398/1‑69 (MQ=255) ccgccCATGCTTTAGCGATCGCCATGCCTTCGCCTTTCGGATCGTTTTCCGGGTGAACGGCAATCAGCg > 1:828330/1‑69 (MQ=255) ccgccCATGCTTTAGCGATCGCCATGCCTTCGCCTTTCGGATCGTTTTCCGGGTGAACGGCAATCAGCg > 1:1354105/1‑69 (MQ=255) ccgccCATGCTTTAGCGATCGCCATGCCTTCGCCTTTCGGATCGTTTTCCGGGTGAACGGCAATCAGCg > 1:1409718/1‑69 (MQ=255) ccgccCATGCTTTAGCGATCGCCATGCCTTCGCCTTTCGGATCGTTTTCCGGGTGAACGGCAATCAGCg > 1:700080/1‑69 (MQ=255) ccgccCATGCTTTAGCGATCGCCATGCCTTCGCCTTTCGGATCGTTTTCCGGGTGAACGGCAATCAGCg > 1:411462/1‑69 (MQ=255) ccgccCATGCTTTAGCGATCGCCATGCCTTCGCCTTTCGGATCGTTTTCCGGGTGAACGGCAATCAGCg > 1:1865606/1‑69 (MQ=255) ccgccCATGCTTTAGCGATCGCCATGCCTTCGCCTTTCGGATCGTTTTCCGGGTGAACGGCAATCAGCg > 1:1964393/1‑69 (MQ=255) ccgccCATGCTTTAGCGATCGCCATGCCTTCGCCTTTCGGATCGTTTTCCGGGTGAACGGCAATCAGCg > 1:1985826/1‑69 (MQ=255) ccgccCATGCTTTAGCGATCGCCATGCCTTCGCCTTTCGGATCGTTTTCCGGGTGAACGGCAATCAGCg > 1:2127307/1‑69 (MQ=255) ccgccCATGCTTTAGCGATCGCCATGCCTTCGCCTTTCGGATCGTTTTCCGGGTGAACGGCAATCAGCg > 1:25988/1‑69 (MQ=255) ccgccCATGCTTTAGCGATCGCCATGCCTTCGCCTTTCGGATCGTTTTCCGGGTGAACGGCAATCAGCg > 1:2768774/1‑69 (MQ=255) ccgccCATGCTTTAGCGATCGCCATGCCTTCGCCTTTCGGATCGTTTTCCGGGTGAACGGCAATCAGCg > 1:2897450/1‑69 (MQ=255) ccgccCAAGCTTTAGCGATCGCCATGCCTTCGCCTTTCGGATCGTTTTCCGGGTGAACGGCAATCAGCg > 1:1566953/1‑69 (MQ=255) ccAGGCTTTGGCAATCGCCATGCCTTCGCCTTTCGGATCGTTTTCCGGGTGAACGGCAATCAGCGTCgg < 1:6598/69‑1 (MQ=255) ccAGGCTTTGGCAATCGCCATGCCTTCGCCTTTCGGATCGTTTTCCGGGTGAACGGCAATCAGCGTCgg < 1:1436140/69‑1 (MQ=255) ccAGGCTTTGGCAATCGCCATGCCTTCGCCTTTCGGATCGTTTTCCGGGTGAACGGCAATCAGCGTCgg < 1:3340005/69‑1 (MQ=255) | TTTCACTTCCGCAACGAAGGACGATTCCAGCACACCCGCACGGTGACCACCGGTTGCAGCCGCCCAGGCTTTGGCAATCGCCATGCCTTCGCCTTTCGGATCGTTTTCCGGGTGAACGGCAATCAGCGTCGG > minE/2439999‑2440130 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |