| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,440,780 | G→A | 41.6% | intergenic (‑137/‑13) | ilvC ← / → ilvY | ketol‑acid reductoisomerase, NAD(P)‑binding/DNA‑binding transcriptional dual regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,440,780 | 0 | G | A | 41.6% | 25.2 / 47.0 | 68 | intergenic (‑137/‑13) | ilvC/ilvY | ketol‑acid reductoisomerase, NAD(P)‑binding/DNA‑binding transcriptional dual regulator |
| Reads supporting (aligned to +/- strand): ref base G (8/31); new base A (19/9); total (27/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.38e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01 | |||||||||||
AAATTTATTGCGGAAATTGATATATTCACAACGTCACATTGCAATTTTTGCAACGTCAACATCGAGGGCTGTCCCTGTGGATTTACGCGATCTGAAAACCTTCCTGCATCTGGCGGAAAGC > minE/2440717‑2440837 | aaaTTTATTGCGGAAATTGATATATTCACAACGTCACATTGCAATTTTTGCAACGTCAACATCGAGGGc < 1:9167/69‑1 (MQ=255)aaaTTTATTGCGGAAATTGATATATTCACAACGTCACATTGCAATTTTTGCAACGTCAACATCGAGGGc < 1:1932812/69‑1 (MQ=255)aaaTTTATTGCGGAAATTGATATATTCACAACGTCACATTGCAATTTTTGCAACGTCAACATCGAGGGc < 1:2774790/69‑1 (MQ=255)aaaTTTATTGCGGAAATTGATATATTCACAACGTCACATTGCAATTTTTGCAACGTCAACATCGAGGGc < 1:1688405/69‑1 (MQ=255)aaaTTTATTGCGGAAATTGATATATTCACAACGTCACATTGCAATTTTTGCAACGTCAACATCGAGGGc < 1:1562806/69‑1 (MQ=255) ttATTGCGGAAATTGATATATTCACAACGTCACATTGCAATTTTTGCAACGTCAACATCGAGGGCTGTc > 1:1637257/1‑69 (MQ=255) ttATTGCGGAAATTGATATATTCACAACGTCACATTGCAATTTTTGCAACGTCAACATCGAGGGCTGTc > 1:1922940/1‑69 (MQ=255) ttATTGCGGAAATTGATATATTCACAACGTCACATTGCAATTTTTGCAACGTCAACATCGAGGGCTGTc < 1:1861678/69‑1 (MQ=255) ttATTGCGGAAATTGATATATTCACAACGTCACATTGCAATTTTTGCAACGTCAACATCGAGGGCTGTc > 1:3240125/1‑69 (MQ=255) ttATTGCGGAAATTGATATATTCACAACGTCACATTGCAATTTTTGCAACGTCAACATCGAGGGCTGTc > 1:2276420/1‑69 (MQ=255) ttATTGCGGAAATTGATATATTCACAACGTCACATTGCAATTTTTGCAACGTCAACATCGAGGGCTGTc > 1:2380069/1‑69 (MQ=255) ttATTGCGGAAATTGATATATTCACAACGTCACATTGCAATTTTTGCAACGTCAACATCGAGGGCTGTc < 1:71414/69‑1 (MQ=255) atatTCACAACGTCACATTGCAATTTTTGCAACGTCAACATCGATGGCTGTCCCTGTGGATTTAcgcg < 1:1067610/68‑1 (MQ=255) atatTCACAACGTCACATTGCAATTTTTGCAACGTCAACATCGAGGGCTGTCCCTGTGGATTTAcgcta < 1:2848497/69‑3 (MQ=255) atatTCACAACGTCACATTGCAATTTTTGCAACGTCAACATCGAGGGCTGTCCCTGTGGATTTAcgcg < 1:2554182/68‑1 (MQ=255) atatTCACAACGTCACATTGCAATTTTTGCAACGTCAACATCGAGGGCTGTCCCTGTGGATTTAcgcg < 1:2305615/68‑1 (MQ=255) atatTCACAACGTCACATTGCAATTTTTGCAACGTCAACATCGAGGGCTGTCCCTGTGGATTTAcgcg < 1:2777371/68‑1 (MQ=255) atatTCACAACGTCACATTGCAATTTTTGCAACGTCAACATCGAGGGCTGTCCCTGTGGATTTAcgcg < 1:2249599/68‑1 (MQ=255) atatTCACAACGTCACATTGCAATTTTTGCAACGTCAACATCGAGGGCTGTCCCTGTGGATTTAcgcg < 1:2052505/68‑1 (MQ=255) atatTCACAACGTCACATTGCAATTTTTGCAACGTCAACATCGAGGGCTGTCCCTGTGGATTTAcgcg < 1:268196/68‑1 (MQ=255) atatTCACAACGTCACATTGCAATTTTTGCAACGTCAACATCGAGGGCTGTCCCTGTGGATTTAcgcg < 1:3051439/68‑1 (MQ=255) atatTCACAACGTCACATTGCAATTTTTGCAACGTCAACATCGAGGGCTGTCCCTGTGGATTTAcgcg < 1:527617/68‑1 (MQ=255) atatTCACAACGTCACATTGCAATTTTTGCAACGTCAACATCGAGGGCTGTCCCTGTGGATTTAcgcg < 1:701379/68‑1 (MQ=255) atatTCACAACGTCACATTGCAATTTTTGCAACGTCAACATCGAGGGCTGTCCCTGTGGATTTAcgcg < 1:1414745/68‑1 (MQ=255) atatTCACAACGTCACATTGCAATTTTTGCAACGTAAACATCGAGGGCTGTCCCTGTGGATTTAcgcg < 1:534321/68‑1 (MQ=255) ttCACAACGTCACATTGCAATTTTTGCAACGTCAACATCGAGGGCTGTCCCTGTGGATTTACTCGAt > 1:3275744/1‑67 (MQ=255) ttCACAACGTCACATTGCAATTTTTGCAACGTCAACATCGAGGGCTGTCCCTGTGGATTTACGCGATc > 1:2897323/1‑68 (MQ=255) aaCGTCACATTGCAATTTTTGCAACGTCAACATCGAGGGCTGTCCCTGTGGATTTACGCGATCTGaaaa < 1:3085975/69‑1 (MQ=255) aaCGTCACATTGCAATTTTTGCAACGTCAACATCGAGGGCTGTCCCTGTGGATTTACGCGATCTGaaaa < 1:3274201/69‑1 (MQ=255) aaCGTCACATTGCAATTTTTGCAACGTCAACATCGAGGGCTGTCCCTGTGGATTTACGCGATCTGaaaa < 1:424284/69‑1 (MQ=255) aaCGTCACATTGCAATTTTTGCAACGTCAACATCGAGGGCTGTCCCTGTGGATTTACGCGATCTGaaaa < 1:1388878/69‑1 (MQ=255) aCGTCACATTGCAATTTTTGCAACGTCAACATCTAGGGCTGTCCCTGTGGATTTACGCGATCTGaaaa < 1:3058280/68‑1 (MQ=255) tGCAATTTTTGCAACGTCAACATCGAGGGCTGTCCCTGTGGATTTACGCGATCTGAAAACCTTCCTGCa < 1:986648/69‑1 (MQ=255) gCAATTTTTGCAACGTCAACATCGAGGGCTGTCCCTGTGGATTTACGCGATCTGAAAACCTTCCTGCa < 1:1080302/68‑1 (MQ=255) gCAATTTTTGCAACGTCAACATCGAGGGCTGTCCCTGTGGATTTACGCGATCTGAAAACCTTCCTGCAt > 1:1921154/1‑69 (MQ=255) aTTTTTGCAACGTCAACATCGAGGGCTGTCCCTGTGGATTTACGCGATCTGAAAACCTTCCTGCATCTg < 1:1704924/69‑1 (MQ=255) aTTTTTGCAACGTCAACATCGAGGGCTGTCCCTGTGGATTTACGCGATCTGAAAACCTTCCTGCATCTg < 1:924086/69‑1 (MQ=255) aTTTTTGCAACGTCAACATCGAGGGCTGTCCCTGTGGATTTACGCGATCTGAAAACCTTCCTGCATCTg < 1:1536382/69‑1 (MQ=255) aTTTTTGCAACGTCAACATCGAGGGCTGTCCCTGTGGATTTACGCGATCTGAAAACCTTCCTGCATCTg < 1:498594/69‑1 (MQ=255) aTTTTTGCAACGTCAACATCGAGGGCTGTCCCTGTGGATTTACGCGATCTGAAAACCTTCCTGCATCTg < 1:444953/69‑1 (MQ=255) cAACGTCAACATTAAGGGCTGCGCCATTGGATTTACGCGAt > 1:891146/1‑41 (MQ=255) cAACGTCAACATTAAGGGCTGCGCCATTGGATTTACGCGATc > 1:1798189/1‑42 (MQ=255) cAACGTCAACATTAAGGGCTGCGCCATTGGATTTACGCGATCt > 1:1664822/1‑43 (MQ=255) cAACGTCAACATTAAGGGCTGCGCCATTGGATTTACGCGATCt > 1:2493854/1‑43 (MQ=255) cAACGTCAACATTAAGGGCTGCGCCATTGGATTTACGCGATCt > 1:923339/1‑43 (MQ=255) cAACGTCAACATTAAGGGCTGCGCCATTGGATTTACGCGATCt > 1:1212658/1‑43 (MQ=255) cAACGTCAACATTAAGGGCTGCGCCATTGGATTTACGCGATCt > 1:881288/1‑43 (MQ=255) cAACGTCAACATTAAGGGCTGCGCCATTGGATTTACGCGATCt > 1:1221992/1‑43 (MQ=255) cAACGTCAACATTAAGGGCTGCGCCATTGGATTTACGCGATCt > 1:1486522/1‑43 (MQ=255) cAACGTCAACATTAAGGGCTGCGCCATTGGATTTACGCGATCt > 1:2354022/1‑43 (MQ=255) cAACGTCAACATTAAGGGCTGCGCCATTGGATTTACGCGATCt > 1:2344025/1‑43 (MQ=255) cAACGTCAACATTAAGGGCTGCGCCATTGGATTTACGCGATCt > 1:200558/1‑43 (MQ=255) cAACGTCAACATTAAGGGCTGCGCCATTGGATTTACGCGATCt > 1:376058/1‑43 (MQ=255) cAACGTCAACATTAAGGGCTGCGCCATTGGATTTACGCGATCt > 1:3382536/1‑43 (MQ=255) cAACGTCAACATTAAGGGCTGCGCCATTGGATTTACGCGATCt > 1:1726577/1‑43 (MQ=255) cAACGTCAACATTAAGGGCTGCGCCATTGGATTTACGCGATCt > 1:3103045/1‑43 (MQ=255) cAACGTCAACATTAAGGGCTGCGCCATTGGATTTACGCGATCt > 1:1814280/1‑43 (MQ=255) cAACGTCAACATTAAGGGCTGCGCCATTGGATTTACGCGATCt > 1:1858880/1‑43 (MQ=255) cAACGTCAACATTAAGGGCTGCGCCATTGGATTTACGCGATCt > 1:2945288/1‑43 (MQ=255) aCGTCAACATTAAGGTCTGCGCCATTGGATTTACGCGATCTGAAAACCTTCCTGCATCTGGCGGAAAgc < 1:434076/69‑1 (MQ=25) aCGTCAACATTAAGGGCTGCGCCATTGGATTTACGCGATCTGAAAACCTTCCTGCATCTGGCTGAAAgc < 1:3404/69‑1 (MQ=255) aCGTCAACATTAAGGGCTGCGCCATTGGATTTACGCGATCTGAAAACCTTCCTGCATCTGGCGGAAAgc < 1:2649123/69‑1 (MQ=25) aCGTCAACATTAAGGGCTGCGCCATTGGATTTACGCGATCTGAAAACCTTCCTGCATCTGGCGGAAAgc < 1:1544860/69‑1 (MQ=25) aCGTCAACATTAAGGGCTGCGCCATTGGATTTACGCGATCTGAAAACCTTCCTGCATCTGGCGGAAAgc < 1:2705439/69‑1 (MQ=25) aCGTCAACATTAAGGGCTGCGCCATTGGATTTACGCGATCTGAAAACCTTCCTGCATCTGGCGGAAAgc < 1:3211018/69‑1 (MQ=25) aCGTCAACATTAAGGGCTGCGCCATTGGATTTACGCGATCTGAAAACCTTCCTGCATCTGGCGGAAAgc < 1:3057633/69‑1 (MQ=25) aCGTCAACATTAAGGGCTGCGCCATTGGATTTACGCGATCTGAAAACCTTCCTGCATCTGGCGGAAAgc < 1:2149214/69‑1 (MQ=25) aCGTCAACATTAAGGGCTGCGCCATTGGATTTACGCGATCTGAAAACCTTCCTGCATCTGGCGGAAAgc < 1:2701718/69‑1 (MQ=25) | AAATTTATTGCGGAAATTGATATATTCACAACGTCACATTGCAATTTTTGCAACGTCAACATCGAGGGCTGTCCCTGTGGATTTACGCGATCTGAAAACCTTCCTGCATCTGGCGGAAAGC > minE/2440717‑2440837 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |