| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,441,545 | C→G | 40.7% | P251P (CCC→CCG) | ilvY → | DNA‑binding transcriptional dual regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,441,545 | 0 | C | G | 40.7% | 43.3 / 78.5 | 81 | P251P (CCC→CCG) | ilvY | DNA‑binding transcriptional dual regulator |
| Reads supporting (aligned to +/- strand): ref base C (18/30); new base G (6/27); total (24/57) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.37e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01 | |||||||||||
GGTATCGATGGTGGCACTCGGCTGTGGCGTGGCATTGTTGCCGGAAGTGGTGCTGGAAAACAGCCCCGAACCGGTGCGTAACCGCGTGATGATTTTAGAGCGCAGCGATGAGAAAACGCCG > minE/2441479‑2441599 | ggTATCGATGGTGGCACTCGGCTGTGGCGTGGCATTGTTGCCGGAAGTGGTGCTGGAAAACAGCCCCGa < 1:447929/69‑1 (MQ=255) gTATCGATGGTGGCACTCGGCTGTGGCGTGGCATTGTTGCCGGAAGTGGTGCTGGAAAACAGCCCCGa < 1:1662394/68‑1 (MQ=255) aTCGATGGTGGCACTCGGCTGTGGCGTGGCATTGTTGCCGGAAGTGGTGCTGGAAAACAGCCCCGAAcc < 1:2724311/69‑1 (MQ=255) aTCGATGGTGGCACTCGGCTGTGGCGTGGCATTGTTGCCGGAAGTGGTGCTGGAAAACAGCCCCGAAcc < 1:708/69‑1 (MQ=255) gtggCACTCGGCTGTGGCGTGGCATTGTTGCCGGAAGTGGTGCTGGAAAACAGCCCCGAACCGGTGCGt < 1:1026634/69‑1 (MQ=255) gtggCACTCGGCTGTGGCGTGGCATTGTTGCCGGAAGTGGTGCTGGAAAACAGCCCCGAACCGGTGCGt < 1:2729279/69‑1 (MQ=255) gtggCACTCGGCTGTGGCGTGGCATTGTTGCCGGAAGTGGTGCTGGAAAACAGCCCCGAACCGGTGCGt < 1:2589971/69‑1 (MQ=255) gtggCACTCGGCTGTGGCGTGGCATTGTTGCCGGAAGTGGTGCTGGAAAACAGCCCCGAACCGGTGCGt < 1:2773875/69‑1 (MQ=255) gtggCACTCGGCTGTGGCGTGGCATTGTTGCCGGAAGTGGTGCTGGAAAACAGCCCCGAACCGGTGCGt < 1:2843084/69‑1 (MQ=255) gtggCACTCGGCTGTGGCGTGGCATTGTTGCCGGAAGTGGTGCTGGAAAACAGCCCCGAACCGGTGCGt < 1:2409656/69‑1 (MQ=255) gtggCACTCGGCTGTGGCGTGGCATTGTTGCCGGAAGTGGTGCTGGAAAACAGCCCCGAACCGGTGCGt < 1:2888802/69‑1 (MQ=255) gtggCACTCGGCTGTGGCGTGGCATTGTTGCCGGAAGTGGTGCTGGAAAACAGCCCCGAACCGGTGCGt < 1:2336081/69‑1 (MQ=255) gtggCACTCGGCTGTGGCGTGGCATTGTTGCCGGAAGTGGTGCTGGAAAACAGCCCCGAACCGGTGCGt < 1:3087962/69‑1 (MQ=255) gtggCACTCGGCTGTGGCGTGGCATTGTTGCCGGAAGTGGTGCTGGAAAACAGCCCCGAACCGGTGCGt < 1:1655432/69‑1 (MQ=255) gtggCACTCGGCTGTGGCGTGGCATTGTTGCCGGAAGTGGTGCTGGAAAACAGCCCCGAACCGGTGCGt < 1:1568117/69‑1 (MQ=255) gtggCACTCGGCTGTGGCGTGGCATTGTTGCCGGAAGTGGTGCTGGAAAACAGCCCCGAACCGGTGCGt < 1:1401346/69‑1 (MQ=255) gtggCACTCGGCTGTGGCGTGGCATTGTTGCCGGAAGTGGTGCTGGAAAACAGCCCCGAACCGGTGCGt < 1:1182029/69‑1 (MQ=255) tggCACTCGGCTGTGGCGTGGCATTGTTGCCGGAAGTGGTGCTGGAAAACAGCCCCGAACCGGTGCGt < 1:709655/68‑1 (MQ=255) ggCACTCGGCTGTGGCGTGGCATTGTTGCCGGAAGTGGTGCTGGAAAACAGCCCCGAACCGGTGCGTaa > 1:3192732/1‑69 (MQ=255) ggCACTCGGCTGTGGCGTGGCATTGTTGCCGGAAGTGGTGCTGGAAAACAGCCCCGAACCGGTGCGTa < 1:2858154/68‑1 (MQ=255) cTCGGCTGTGGCGTGGCATTGTTGCCGGAAGTGGTGCTGGAAAACAGCCCCGa < 1:1989006/53‑1 (MQ=255) cGGCTGTGGCGTGGCATTGTTGCCGGAAGTGGTGCTGGAAAACAGCCCCGAACCGGTGCGTAACCGCGt > 1:1701887/1‑69 (MQ=255) ttgttgCCGGAAGTGGTGCTGGAAAACAGCCCCGAACCGGTGCGTAACCGCGTGATGATTTTAGAgcgc > 1:1801307/1‑69 (MQ=255) ttgttgCCGGAAGTGGTGCTGGAAAACAGCCCCGAACCGGTGCGTAACCGCGTGATGATTTTAGAgcgc > 1:3184378/1‑69 (MQ=255) ttgttgCCGGAAGTGGTGCTGGAAAACAGCCCCGAACCGGTGCGTAACCGCGTGATGATTTTAGAgcgc > 1:2737910/1‑69 (MQ=255) ttgttgCCGGAAGTGGTGCTGGAAAACAGCCCCGAACCGGTGCGTAACCGCGTGATGATTTTAGAgcgc > 1:80768/1‑69 (MQ=255) ttgttgCCGGAAGTGGTGCTGGAAAACAGCCCCGAACCGGTGCGTAACCGCGTGATGATTTTAGAgcgc > 1:2703777/1‑69 (MQ=255) ttgttgCCGGAAGTGGTGCTGGAAAACAGCCCCGAACCGGTGCGTAACCGCGTGATGATTTTAGAgcg > 1:2211844/1‑68 (MQ=255) tgttgCCGGAAGTGGTGCTGTAAAACAGCCCCGAAcc < 1:145610/37‑1 (MQ=255) tttgCCGGAAGCGGTGCTGGAAAACAGCCCGGAACCGGTGCGTAACCGCGTGATGATTTTAGAGCGCAg < 1:3104395/68‑1 (MQ=255) gttgCCGGAAGTGGTGCTGGAAAACAGCCCCGAACCGGTGCGTAACCGCGTGATGATTTTAGAGCGCa > 1:2861258/1‑68 (MQ=255) gttgCCGGAAGTGGTGCTGGAAAACAGCCCCGAACCGGTGCGTAACCGCGTGATGATTTTAGAGCGCa > 1:1319843/1‑68 (MQ=255) gttgCCGGAAGTGGTGCTGGAAAACAGCCCCGAACCGGTGCGTAACCGCGTGATGATTTTAGAGCGCa > 1:2849882/1‑68 (MQ=255) gttgCCGGAAGTGGTGCTGGAAAACAGCCCCGAACCGGTGCGTAACCGCGTGATGATTTTAGAGCGCa > 1:2253960/1‑68 (MQ=255) gttgCCGGAAGTGGTGCTGGAAAACAGCCCCGAACCGGTGCGTAACCGCGTGATGATTTTAGAGCGCa > 1:2161613/1‑68 (MQ=255) tgCCGGAAGTGGTGCTGGAAAACAGCCCGGAACCGGTGCGTAACCGCGTGATGATTTTAGCGCGCAg < 1:439388/67‑1 (MQ=255) tgCCGGAAGTGGTGCTGGAAAACAGCCCGGAACCGGTGCGTAACCGCGTGATGATTTTAGAGCGCAg < 1:79437/67‑1 (MQ=255) tgCCGGAAGTGGTGCTGGAAAACAGCCCGGAACCGGTGCGTAACCGCGTGATGATTTTAGAGCGCAg < 1:873578/67‑1 (MQ=255) tgCCGGAAGTGGTGCTGGAAAACAGCCCGGAACCGGTGCGTAACCGCGTGATGATTTTAGAGCGCAg < 1:95335/67‑1 (MQ=255) tgCCGGAAGTGGTGCTGGAAAACAGCCCGGAACCGGTGCGTAACCGCGTGATGATTTTAGAGCGCAg < 1:971724/67‑1 (MQ=255) tgCCGGAAGTGGTGCTGGAAAACAGCCCGGAACCGGTGCGTAACCGCGTGATGATTTTAGAGCGCAg < 1:2981827/67‑1 (MQ=255) tgCCGGAAGTGGTGCTGGAAAACAGCCCGGAACCGGTGCGTAACCGCGTGATGATTTTAGAGCGCAg < 1:3136864/67‑1 (MQ=255) tgCCGGAAGTGGTGCTGGAAAACAGCCCGGAACCGGTGCGTAACCGCGTGATGATTTTAGAGCGCAg < 1:3175526/67‑1 (MQ=255) tgCCGGAAGTGGTGCTGGAAAACAGCCCGGAACCGGTGCGTAACCGCGTGATGATTTTAGAGCGCAg < 1:3230430/67‑1 (MQ=255) tgCCGGAAGTGGTGCTGGAAAACAGCCCGGAACCGGTGCGTAACCGCGTGATGATTTTAGAGCGCAg < 1:568680/67‑1 (MQ=255) tgCCGGAAGTGGTGCTGGAAAACAGCCCGGAACCGGTGCGTAACCGCGTGATGATTTTAGAGCGCAg < 1:3350064/67‑1 (MQ=255) tgCCGGAAGTGGTGCTGGAAAACAGCCCGGAACCGGTGCGTAACCGCGTGATGATTTTAGAGCGCAg < 1:3404830/67‑1 (MQ=255) tgCCGGAAGTGGTGCTGGAAAACAGCCCGGAACCGGTGCGTAACCGCGTGATGATTTTAGAGCGCAg < 1:2512092/67‑1 (MQ=255) tgCCGGAAGTGGTGCTGGAAAACAGCCCGGAACCGGTGCGTAACCGCGTGATGATTTTAGAGCGCAg < 1:1131230/67‑1 (MQ=255) tgCCGGAAGTGGTGCTGGAAAACAGCCCGGAACCGGTGCGTAACCGCGTGATGATTTTAGAGCGCAg < 1:1494706/67‑1 (MQ=255) tgCCGGAAGTGGTGCTGGAAAACAGCCCGGAACCGGTGCGTAACCGCGTGATGATTTTAGAGCGCAg < 1:1530992/67‑1 (MQ=255) tgCCGGAAGTGGTGCTGGAAAACAGCCCGGAACCGGTGCGTAACCGCGTGATGATTTTAGAGCGCAg < 1:1560304/67‑1 (MQ=255) tgCCGGAAGTGGTGCTGGAAAACAGCCCGGAACCGGTGCGTAACCGCGTGATGATTTTAGAGCGCAg < 1:1039122/67‑1 (MQ=255) tgCCGGAAGTGGTGCTGGAAAACAGCCCGGAACCGGTGCGTAACCGCGTGATGATTTTAGAGCGCAg < 1:166405/67‑1 (MQ=255) tgCCGGAAGTGGTGCTGGAAAACAGCCCGGAACCGGTGCGTAACCGCGTGATGATTTTAGAGCGCAg < 1:2280802/67‑1 (MQ=255) tgCCGGAAGTGGTGCTGGAAAACAGCCCGGAACCGGTGCGTAACCGCGTGATGATTTTAGAGCGCAg < 1:2315472/67‑1 (MQ=255) tgCCGGAAGTGGTGCTGGAAAACAGCCCGGAACCGGTGCGTAACCGCGTGATGATTTTAGAGCGCAg < 1:1030911/67‑1 (MQ=255) tgCCGGAAGTGGTGCTGGAAAACAGCCCGGAACCGGTGCGTAACCGCGTGATGATTTTAGAGCGCAg < 1:2444301/67‑1 (MQ=255) tgCCGGAAGTGGTGCTGGAAAACAGCCCGGAACCGGTGCGTAACCGCGTGATGATTTTAGAGCGCAg < 1:2358525/67‑1 (MQ=255) tgCCGGAAGTGGTGCTGGAAAACAGCCCCGAACCGGTGCGTGACCGCGTGATGATTTTAGAGCGCAGc < 1:2503969/68‑1 (MQ=255) tgCCGGAAGTGGTGCTGGAAAACAGCCCCGAACCGGTGCGTAACCGCGTGATGATTTTAGAGCGCAGc < 1:1954722/68‑1 (MQ=255) tgCCGGAAGTGGTGCTGGAAAACAGCCCCGAACCGGTGCGTAACCGCGTGATGATTTTAGAGCGCAGc < 1:1559192/68‑1 (MQ=255) tgCCGGAAGTGGTGCTGGAAAACAGCCCCGAACCGGTGCGTAACCGCGTGATGATTTTAGAGCGCAGc < 1:1604588/68‑1 (MQ=255) tgCCGGAAGTGGTGCTGGAAAACAGCCCCGAACCGGTGCGTAACCGCGTGATGATTTTAGAGCGCAGc < 1:1657360/68‑1 (MQ=255) tgCCGGAAGTGGTGCTGGAAAACAGCCCCGAACCGGTGCGTAACCGCGTGATGATTTTAGAGCGCAGc < 1:1637823/68‑1 (MQ=255) tgCCGGAAGTGGTGCTGGAAAACAGCCCCGAACCGGTGCGTAAACGCGTGATGATTTTAGAGCGCAGc < 1:2345216/68‑1 (MQ=255) gCCGGAAGTGGTGCTGGAAAACAGCCCGGAACCGGTGCGTAACCGCGTGATGATTTTAGAGCGCAg < 1:1916081/66‑1 (MQ=255) gCCGGAAGTGGTGCTGGAAAACAGCCCCGAACCGGTGCGTAACCGCGTGATGATTTTAGAGCGCAGCg > 1:2878622/1‑68 (MQ=255) gCCGGAAGTGGTGCTGGAAAACAGCCCCGAACCGGTGCGTAACCGCGTGATGATTTTAGAGCGCAGCg > 1:1842918/1‑68 (MQ=255) gCCGGAAGTGGTGCTGGAAAACAGCCCCGAACCGGTGCGTAACCGCGTGATGATTTTAGAGCGCAGCg > 1:941343/1‑68 (MQ=255) gCCGGAAGTGGTGCTGGAAAACAGCCCCGAACCGGTGCGTAACCGCGTGATGATTTTAGAGCGCAGCg > 1:2966672/1‑68 (MQ=255) gCCGGAAGTGGTGCTGGAAAACAGCCCCGAACCGGTGCGTAACCGCGTGATGATTTTAGAGCGCAGCg > 1:1301268/1‑68 (MQ=255) ggAAGTGGTGCTGGAAAACAGCCCGGAACCGGTGCGTAACCGCGTGATGATTTTAGAGCGAAg < 1:1115680/63‑1 (MQ=255) aGTGGTGCTGGAAAACAGCCCCGAACCGGTGCGTAACCGCGTGATGATTTTAGAGCGCAGCGATGAGaa < 1:567387/69‑1 (MQ=255) aGTGGTGCTGGAAAACAGCCCCGAACCGGTGCGTAACCGCGTGATGATTTTAGAGCGCAGCGATGAGaa < 1:752510/69‑1 (MQ=255) gAAAACAGCCCGGACCCGGTGCGTAACCGCGTGATGATTTTAGAGCGCAGtgatga > 1:599073/1‑56 (MQ=255) gAAAACAGCCCGGAACCGGTGCGTAACCGCGTGATGATTTTAGAGCGCAGTGATGAAAAAACGCCg > 1:1004873/1‑66 (MQ=255) gAAAACAGCCCGGAACCGGTGCGTAACCGCGTGATGATTTTAGAGCGCAGTGATGAAAAAACGCCg > 1:3308596/1‑66 (MQ=255) gAAAACAGCCCGGAACCGGTGCGTAACCGCGTGATGATTTTAGAGCGCAGTGATGAAAAAACGCCg > 1:2238468/1‑66 (MQ=255) gAAAACAGCCCGGAACCGGTGCGTAACCGCGTGATGATTTTAGAGCGCAGTGATGAAAAAACGCCg > 1:2468619/1‑66 (MQ=255) gAAAACAGCCCGGAACCGGTGCGTAACCGCGGGATGATTTTAGAGCGCAGTGATGAAAAAACGCCg > 1:2529895/1‑66 (MQ=255) | GGTATCGATGGTGGCACTCGGCTGTGGCGTGGCATTGTTGCCGGAAGTGGTGCTGGAAAACAGCCCCGAACCGGTGCGTAACCGCGTGATGATTTTAGAGCGCAGCGATGAGAAAACGCCG > minE/2441479‑2441599 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |