| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,443,486 | G→A | 61.4% | D550D (GAC→GAT) | ilvD ← | dihydroxyacid dehydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,443,486 | 0 | G | A | 61.4% | 18.2 / 26.9 | 44 | D550D (GAC→GAT) | ilvD | dihydroxyacid dehydratase |
| Reads supporting (aligned to +/- strand): ref base G (4/13); new base A (12/15); total (16/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.08e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01 | |||||||||||
TCCTGCGCTTCACGACGCGCCGCCAGTTCGGCATCGCTTACCTGTAACTGAATGCCACGGTTCGGGATGTCGATAGCGATCAGGTCACCATCTTCAATCAGGCCAATGCTGCCGCCGCTTGCCGCT > minE/2443418‑2443543 | tacTGCGCTTCACGACGCGCCGCCAGTTCGGCATCGCTTACCTGTAACTGAATGCCACGGTTCGGGATg > 1:1681278/3‑69 (MQ=255)tCCTGCGCTTCACGACGCGCCGCCAGTTCGGCATCGCTTACCTGTAACTGAATGCCACGGTTCGGGATg > 1:1088104/1‑69 (MQ=255)tCCTGCGCTTCACGACGCGCCGCCAGTTCGGCATCGCTTACCTGTAACTGAATGCCACGGTTCGGGATg > 1:1092173/1‑69 (MQ=255)tCCTGCGCTTCACGACGCGCCGCCAGTTCGGCATCGCTTACCTGTAACTGAATGCCACGGTTCGGGATg > 1:774055/1‑69 (MQ=255)tCCTGCGCTTCACGACGCGCCGCCAGTTCGGCATCGCTTACCTGTAACTGAATGCCACGGTTCGGGATg > 1:3107954/1‑69 (MQ=255)tCCTGCGCTTCACGACGCGCCGCCAGTTCGGCATCGCTTACCTGTAACTGAATGCCACGGTTCGGGATg > 1:305395/1‑69 (MQ=255)tCCTGCGCTTCACGACGCGCCGCCAGTTCGGCATCGCTTACCTGTAACTGAATGCCACGGTTCGGGATg > 1:2665052/1‑69 (MQ=255)tCCTGCGCTTCACGACGCGCCGCCAGTTCGGCATCGCTTACCTGTAACTGAATGCCACGGTTCGGGATg > 1:1443282/1‑69 (MQ=255) cTGCGCTTCACGACGCGCCGCCAGTTCGGCATCGCTTACCTGTAACTGAATGCCACGGTTCGGGATGTc < 1:2403256/69‑1 (MQ=255) cTGCGCTTCACGACGCGCCGCCAGTTCGGCATCGCTTACCTGTAACTGAATGCCACGGTTCGGGATGTc < 1:1214242/69‑1 (MQ=255) acgCGCCGCCAGTTCGGCATCGCTTACCTGTAACTGAATGCCACGGTTCGGGATGTCGATAGCGATCAg < 1:12803/69‑1 (MQ=255) acgCGCCGCCAGTTCGGCATCGCTTACCTGTAACTGAATGCCACGGTTCGGGATGTCGATAGCGATCAg < 1:1488539/69‑1 (MQ=255) acgCGCCGCCAGTTCGGCATCGCTTACCTGTAACTGAATGCCACGGTTCGGGATGTCGATAGCGATCAg < 1:1587216/69‑1 (MQ=255) acgCGCCGCCAGTTCGGCATCGCTTACCTGTAACTGAATGCCACGGTTCGGGATGTCGATAGCGATCAg < 1:2616869/69‑1 (MQ=255) acgCGCCGCCAGTTCGGCATCGCTTACCTGTAACTGAATGCCACGGTTCGGGATGTCGATAGCGATCAg < 1:856003/69‑1 (MQ=255) acgCGCCGCCAGTTCGGCATCGCTTACCTGTAACTGAATGCCACGGTTCGGGATGTCGATAGCGATCAg < 1:724254/69‑1 (MQ=255) acgCGCCGCCAGTTCGGCATCGCTTACCTGTAACTGAATGCCACGGTTCGGGATGTCGATAGCGATCAg < 1:631384/69‑1 (MQ=255) acgCGCCGCCAGTTCGGCATCGCTTACCTGTAACTGAATGCCACGGTTCGGGATGTCGATAGCGATCAg < 1:2353894/69‑1 (MQ=255) ccgccAGTTCGGCATCGCTTACCTGTAACTTAATGCCTCGGTTCGGAATATCGa < 1:362412/54‑1 (MQ=255) ccgccAGTTCGGCATCGCTTACCTGTAACTGAATGCCTCGGTTCGGAATATCGa < 1:2104360/54‑1 (MQ=255) ccgccAGTTCGGCATCGCTTACCTGTAACTGAATGCCTCGGTTCGGAATATCGa < 1:1179591/54‑1 (MQ=255) ccgccAGTTCGGCATCGCTTACCTGTAACTGAATGCCTCGGTTCGGAATATCGa < 1:444831/54‑1 (MQ=255) ccgccAGTTCGGCATCGCTTACCTGTAACTGAATGCCTCGGTTCGGAATATCGa < 1:2661596/54‑1 (MQ=255) ccgccAGTTCGGCATCGCTTACCTGTAACTGAATGCCTCGGTTCGGAATATCGa < 1:2410952/54‑1 (MQ=255) ccgccAGTTCGGCATCGCTTACCTGTAACTGAATGCCTCGGTTCGGAATATCGa < 1:683089/54‑1 (MQ=255) ccgccAGTTCGGCATCGCTTACCTGTAACTGAATGCCTCGGTTCGGAATATCGa < 1:2228461/54‑1 (MQ=255) ccgccAGTTCGGCATCGCTTACCTGTAACTGAATGCCTCGGTTCGGAATATCGa < 1:2224484/54‑1 (MQ=255) ccgccAGTTCGGCATCGCTTACCTGTAACTGAATGCCTCGGTTCGGAATATCGa < 1:713795/54‑1 (MQ=255) ccgccAGTTCGGCATCGCTTACCTGTAACTGAATGCCTCGGTTCGGAATATCGa < 1:169455/54‑1 (MQ=255) ccgccAGTTCGGCATCGCTTACCTGTAACTGAATGCCTCGGTTCGGAATATCGa < 1:161710/54‑1 (MQ=255) ccgccAGTTCGGCATCGCTTACCTGTAACTGAATGCCTCGGTTCGGAATATCGa < 1:2738976/54‑1 (MQ=255) ccgccAGTTCGGCATCGCTTACCTGTAACTGAATGCCTCGGTTCGGAATATCGa < 1:1541594/54‑1 (MQ=255) ccgccAGTTCGGCATCGCTTACCTGTAACTGAATGCCTCGGTTCGGAATATCGa < 1:2894550/54‑1 (MQ=255) tCGCTTACCTGTAACTGAATGCCACGGTTCGGGATGTCGATAGCGATCAGGTCACCATc > 1:313068/1‑59 (MQ=255) gCTTACCTGTAACTGAATGCCACGGTTCGGGATGTCGATAGCGATCAGGTCCCCATCTTCAATCAGGcc > 1:1588842/1‑69 (MQ=255) gTAACTGAATGCCTCGGTTCGGAATATCGATAGCGATCAGGTCACCATCTTCAATCAGGCCAAtgctgc > 1:3383455/1‑69 (MQ=255) gTAACTGAATGCCTCGGTTCGGAATATCGATAGCGATCAGGTCACCATCTTCAATCAGGCCAAtgctgc > 1:1283211/1‑69 (MQ=255) gTAACTGAATGCCTCGGTTCGGAATATCGATAGCGATCAGGTCACCATCTTCAATCAGGCCAAtgctgc > 1:973794/1‑69 (MQ=255) gTAACTGAATGCCTCGGTTCGGAATATCGATAGCGATCAGGTCACCATCTTCAATCAGGCCAAtgctgc > 1:1171272/1‑69 (MQ=255) gTAACTGAATGCCTCGGTTCGGAATATCGATAGCGATCAGGTCACCATCTTCAATCAGGCCAAtgctgc > 1:2978062/1‑69 (MQ=255) gTAACTGAATGCCTCGGTTCGGAATATCGATAGCGATCAGGTCACCATCTTCAATCAGGCCAAtgctgc > 1:2807249/1‑69 (MQ=255) gTAACTGAATGCCTCGGTTCGGAATATCGATAGCGATCAGGTCACCATCTTCAATCAGGCCAAtgctgc > 1:2693578/1‑69 (MQ=255) gTAACTGAATGCCTCGGTTCGGAATATCGATAGCGATCAGGTCACCATCTTCAATCAGGCCAAtgctgc > 1:2484203/1‑69 (MQ=255) gTAACTGAATGCCTCGGTTCGGAATATCGATAGCGATCAGGTCACCATCTTCAATCAGGCCAAtgctgc > 1:1911051/1‑69 (MQ=255) gTAACTGAATGCCTCGGTTCGGAATATCGATAGCGATCAGGTCACCATCTTCAATCAGGCCAAtgctgc > 1:1329485/1‑69 (MQ=255) gTAACTGAATGCCTCGGTTCGGAATATCGATAGCGATCAGGTCACCATCTTCAATCAGGCCAAtgctg > 1:2663565/1‑68 (MQ=255) gTAACTCAATGCCTCGGTTCGGAATATCGATAGCGATCAGGTCACCATCTTCAATCAGACCAAtgctgc > 1:2197495/1‑69 (MQ=255) aCTGAATGCCACGGTTCGGGATGTCGATAGCGATCAGGTCACCATCTTCAATCAGGcc > 1:2072258/1‑58 (MQ=255) aCTGAATGCCACGGTTCGGGATGTCGATAGCGATCAGGTCACCATCTTCAATCAGGCCAATGCTgccgc > 1:100129/1‑69 (MQ=255) tGAATGCCACGGTTCGGGATGTCGATAGCGATCAg < 1:1163533/35‑1 (MQ=255) cGGTTCGGGATGTCGATAGCGATCAGGTCACCATCTTCAATCAGGCCAATGCTGCCgccgcttgccgct < 1:659725/69‑1 (MQ=255) cGGTTCGGGATGTCGATAGCGATCAGGTCACCATCTTCAATCAGGCCAATGCTGCCgccgcttgccgct < 1:1108441/69‑1 (MQ=255) | TCCTGCGCTTCACGACGCGCCGCCAGTTCGGCATCGCTTACCTGTAACTGAATGCCACGGTTCGGGATGTCGATAGCGATCAGGTCACCATCTTCAATCAGGCCAATGCTGCCGCCGCTTGCCGCT > minE/2443418‑2443543 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |