Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,444,281 C→T 23.5% L285L (CTG→CTA ilvD ← dihydroxyacid dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,444,2810CT23.5% 175.3 / 43.0 132L285L (CTG→CTAilvDdihydroxyacid dehydratase
Reads supporting (aligned to +/- strand):  ref base C (41/59);  new base T (9/22);  total (50/82)
Fisher's exact test for biased strand distribution p-value = 2.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 133 total aligned reads displayed.

GGGAAAGCTTATCGATATCACTCATGGTGAAGTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAGTCGATCCACCCATCGCGATATCCAGCGTCATGGCGTTTTCA  >  minE/2444220‑2444341
                                                             |                                                            
gggAAAGCTTATCGATATCACTCATGGTGAAGTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTg                                                         <  1:2436149/67‑1 (MQ=255)
gggAAAGCTTATCGATATCACTCATGGTGAAGTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTg                                                         <  1:1172757/67‑1 (MQ=255)
         tATCGATATCACTCATGGTGAAGTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGgtgt                                              <  1:169910/69‑1 (MQ=255)
         tATCGATATCACTCATGGTGAAGTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGgtgt                                              <  1:838515/69‑1 (MQ=255)
         tATCGATATCACTCATGGTGAAGTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGgtgt                                              <  1:2898805/69‑1 (MQ=255)
          aTCGATATCACTCATGGTGAAGTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGtt                                             >  1:3080372/1‑69 (MQ=255)
          aTCGATATCACTCATGGTGAAGTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGtt                                             >  1:582587/1‑69 (MQ=255)
               tatCACTCATGGTGAAGTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAGTCg                                        <  1:2245499/69‑1 (MQ=255)
               tatCACTCATGGTGAAGTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAGTCg                                        <  1:2250569/69‑1 (MQ=255)
               tatCACTCATGGTGAAGTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAGTCg                                        <  1:2649050/69‑1 (MQ=255)
               tatCACTCATGGTGAAGTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAGTCg                                        <  1:3313666/69‑1 (MQ=255)
               tatCACTCATGGTGAAGTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAGTCg                                        <  1:40055/69‑1 (MQ=255)
               tatCACTCATGGTGAAGTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAGTCg                                        <  1:542765/69‑1 (MQ=255)
               tatCACTCATGGTGAAGTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAGTCg                                        <  1:1052933/69‑1 (MQ=255)
                atCACTGATGGTGAAGTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAGTCGa                                       <  1:1708743/69‑1 (MQ=255)
                atCACTCATGGTGAAGTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAGTCGa                                       <  1:1250277/69‑1 (MQ=255)
                atCACTCATGGTGAAGTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAGTCGa                                       <  1:1145277/69‑1 (MQ=255)
                atCACTCATGGTGAAGTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAGTCGa                                       <  1:1120138/69‑1 (MQ=255)
                atCACTCATGGTGAAGTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAGTCGa                                       <  1:870917/69‑1 (MQ=255)
                atCACTCATGGTGAAGTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAGTCGa                                       <  1:2525448/69‑1 (MQ=255)
                   aCTCATGGTGAAGTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAg                                           >  1:1342026/1‑62 (MQ=255)
                   aCTCATGGTGAAGTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAg                                           >  1:2344439/1‑62 (MQ=255)
                   aCTCATGGTGAAGTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAg                                           >  1:2373600/1‑62 (MQ=255)
                   aCTCATGGTGAAGTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAg                                           >  1:2519358/1‑62 (MQ=255)
                   aCTCATGGTGAAGTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAg                                           >  1:2572706/1‑62 (MQ=255)
                   aCTCATGGTGAAGTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAGTCGATcc                                    >  1:2696309/1‑69 (MQ=255)
                   aCTCATGGTGAAGTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAGTCGATcc                                    >  1:2466317/1‑69 (MQ=255)
                   aCTCATGGTGAAGTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAGTCGATcc                                    >  1:908091/1‑69 (MQ=255)
                   aCTCATGGTGAAGTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAGTCGATcc                                    >  1:2866136/1‑69 (MQ=255)
                   aCTCATGGTGAAGTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAGTCGATcc                                    >  1:830657/1‑69 (MQ=255)
                   aCTCATGGTGAAGTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAGTCGATcc                                    >  1:2276012/1‑69 (MQ=255)
                   aCTCATGGTGAAGTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAGTCGATcc                                    >  1:613927/1‑69 (MQ=255)
                   aCTCATGGTGAAGTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAGTCGATcc                                    >  1:975462/1‑69 (MQ=255)
                   aCTCATGGTGAAGTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAGTCGATcc                                    >  1:1791657/1‑69 (MQ=255)
                   aCTCATGGTGAAGTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAGTCGATcc                                    >  1:1067960/1‑69 (MQ=255)
                   aCTCATGGTGAAGTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAGTCGATcc                                    >  1:1960202/1‑69 (MQ=255)
                   aCTCATGGTGAAGTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAGTCGATcc                                    >  1:1587010/1‑69 (MQ=255)
                   aCTCATGGTGAAGTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAGTCGATc                                     >  1:2887725/1‑68 (MQ=255)
                     tCATGGTGAAGTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAGTCGATCCa                                   <  1:930253/68‑1 (MQ=255)
                     tCATGGTGAAGTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAGTCGATCCa                                   <  1:476564/68‑1 (MQ=255)
                     tCATGGTGAAGTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAGTCGATCCa                                   <  1:365171/68‑1 (MQ=255)
                          gTGAAGTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAGTCGATCCACCCa                               <  1:491877/67‑1 (MQ=255)
                          gTGAAGTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAGTCGATCCACCCa                               <  1:445741/67‑1 (MQ=255)
                            gAAGTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAGTCGATCCACCCATcgc                           <  1:662178/69‑1 (MQ=255)
                            gAAGTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAGTCGATCCACCCATcgc                           <  1:3133795/69‑1 (MQ=255)
                             aaGTCGATTTCCTATTCCTTCGAAGACGCCAGCAGGt                                                          >  1:1038084/1‑37 (MQ=12)
                             aaGTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAGTCGATCCACCCATcgc                           >  1:3330771/1‑68 (MQ=255)
                             aaGTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAGTCGATCCACCCATcgc                           >  1:3098995/1‑68 (MQ=255)
                             aaGTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAGTCGATCCACCCATcgc                           >  1:1387033/1‑68 (MQ=255)
                             aaGTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAGTCGATCCACCCATcgc                           >  1:1264942/1‑68 (MQ=255)
                             aaGTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAGTCGATCCACCCATcgc                           >  1:3190702/1‑68 (MQ=255)
                             aaGTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAGTCGATCCACCCATcgc                           >  1:2799613/1‑68 (MQ=255)
                             aaGTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAGTCGATCCACCCATcgc                           >  1:2553573/1‑68 (MQ=255)
                               gTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAGTCGATCCACCCATCGCGat                        <  1:905273/69‑1 (MQ=255)
                               gTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAGTCGATCCACCCATCGCGat                        >  1:25295/1‑69 (MQ=255)
                               gTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAGTCGATCCACCCATCGCGat                        <  1:1856942/69‑1 (MQ=255)
                               gTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAGTCGATCCACCCATCGCGat                        <  1:259324/69‑1 (MQ=255)
                               gTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAGTCGATCCACCCATCGCGat                        <  1:1217358/69‑1 (MQ=255)
                               gTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAGTCGATCCACCCATCGCGat                        <  1:3060892/69‑1 (MQ=255)
                               gTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAGTCGATCCACCCATCGCGat                        >  1:1531284/1‑69 (MQ=255)
                               gTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAGTCGATCCACCCATCGCGat                        >  1:58100/1‑69 (MQ=255)
                               gTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAGTCGATCCACCCATCGCGat                        <  1:3132633/69‑1 (MQ=255)
                               gTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAGTCGATCCACCCATCGCGat                        <  1:2029243/69‑1 (MQ=255)
                               gTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAGTCGATCCACCCATCGCGat                        <  1:15070/69‑1 (MQ=255)
                               gTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAGTCGATCCACCCATCGCGat                        <  1:248414/69‑1 (MQ=255)
                               gTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAGTCGATCCACCCATCGCGat                        <  1:3280427/69‑1 (MQ=255)
                                  gATTTCCGCTTCCTGCGCCGCCGCCAGTAGGTGAAGAACGgtgt                                              >  1:425013/1‑44 (MQ=38)
                                  gATTTCCGCTTCCTGCGCCGCCGCCAGTAGGTGAAGAACGgtgt                                              >  1:286267/1‑44 (MQ=38)
                                  gATTTCCGCTTCCTGCGCCGCCGCCAGTAGGTGAAGAACGgtgt                                              >  1:2410223/1‑44 (MQ=38)
                                  gATTTCCGCTTCCTGCGCCGCCGCCAGTAGGTGAAGAACGgtgt                                              >  1:672757/1‑44 (MQ=38)
                                  gATTTCCGCTTCCTGCGCCGCCGCCAGTAGGTGAAGAACGgtgt                                              >  1:2192215/1‑44 (MQ=38)
                                  gATTTCCGCTTCCTGCGCCGCCGCCAGTAGGTGAAGAACGgtgt                                              >  1:1619284/1‑44 (MQ=38)
                                  gATTTCCGCTTCCTGCGCCGCCGCCAGTAGGTGAAGAACGgtgt                                              >  1:3188165/1‑44 (MQ=38)
                                  gATTTCCGCTTCCTGCGCCGCCGCCAGTAGGTGAAGAACGgtgt                                              >  1:3015099/1‑44 (MQ=38)
                                    tttCCGCTTCCTGCGCCGCCGCCAGTATGTGAAGAACGGTGTTGGt                                          <  1:216306/46‑1 (MQ=37)
                                    tttCCGCTTCCTGCGCCGCCGCCAGTAGGTGAAGAACGGTGTTGGt                                          <  1:386797/46‑1 (MQ=38)
                                    tttCCGCTTCCTGCGCCGCCGCCAGTAGGTGAAGAACGGTGTTGGt                                          <  1:2102525/46‑1 (MQ=38)
                                    tttCCGCTTCCTGCGCCGCCGCCAGTAGGTGAAGAACGGTGTTGGt                                          <  1:107319/46‑1 (MQ=38)
                                    tttCCGCTTCCTGCGCCGCCGCCAGTAGGTGAAGAACGGTGTTGGt                                          <  1:1132916/46‑1 (MQ=38)
                                    tttCCGCTTCCTGCGCCGCCGCCAGTAGGTGAAGAACGGTGTTGGt                                          <  1:1185810/46‑1 (MQ=38)
                                    tttCCGCTTCCTGCGCCGCCGCCAGTAGGTGAAGAACGGTGTTGGt                                          <  1:774851/46‑1 (MQ=38)
                                    tttCCGCTTCCTGCGCCGCCGCCAGTAGGTGAAGAACGGTGTTGGt                                          <  1:684117/46‑1 (MQ=38)
                                    tttCCGCTTCCTGCGCCGCCGCCAGTAGGTGAAGAACGGTGTTGGt                                          <  1:1937471/46‑1 (MQ=38)
                                    tttCCGCTTCCTGCGCCGCCGCCAGTAGGTGAAGAACGGTGTTGGt                                          <  1:634495/46‑1 (MQ=38)
                                    tttCCGCTTCCTGCGCCGCCGCCAGTAGGTGAAGAACGGTGTTGGt                                          <  1:1923346/46‑1 (MQ=38)
                                    tttCCGCTTCCTGCGCCGCCGCCAGTAGGTGAAGAACGGTGTTGGt                                          <  1:1990353/46‑1 (MQ=38)
                                    tttCCGCTTCCTGCGCCGCCGCCAGTAGGTGAAGAACGGTGTTGGt                                          <  1:934388/46‑1 (MQ=38)
                                    tttCCGCTTCCTGCGCCGCCGCCAGTAGGTGAAGAACGGTGTTGGt                                          <  1:3413763/46‑1 (MQ=38)
                                    tttCCGCTTCCTGCGCCGCCGCCAGTAGGTGAAGAACGGTGTTGGt                                          <  1:1956691/46‑1 (MQ=38)
                                    tttCCGCTTCCTGCGCCGCAGCCAGTAGGTGAAGAACGGTGTTGGt                                          <  1:1251074/46‑1 (MQ=25)
                                       ccGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAGTCGATCCACCCATCGCGATATCCAGc                 >  1:2882055/1‑68 (MQ=255)
                                         gCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAGTCGATCCACCCATCGCGATATCCAGCGt               <  1:208747/68‑1 (MQ=255)
                                         gCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAGTCGATCCACCCATCGCGATATCCAGCGt               <  1:72056/68‑1 (MQ=255)
                                         gCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAGTCGATCCACCCATCGCGATATCCAGCGt               <  1:3173386/68‑1 (MQ=255)
                                         gCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAGTCGATCCACCCATCGCGATATCCAGCGt               <  1:892992/68‑1 (MQ=255)
                                             ccTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAGTCGATCCACCCATCGCGATATCCAGCGTCATgg          >  1:744583/1‑69 (MQ=255)
                                             ccTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAGTCGATCCACCCATCGCGATATCCAGCGTCATgg          >  1:467231/1‑69 (MQ=255)
                                                     gccgccAGCAGGTGAAGTACGGTGTTAGTCGATCCACCCATCGCGATATCCAGCGTCATGGCGTTTTCa  <  1:671824/69‑1 (MQ=255)
                                                     gccgccAGCAGGTGAAGTACGGTGTTAGTCGATCCACCCATCGCGATATCCAGCGTCATGGCGTTTTCa  <  1:224176/69‑1 (MQ=255)
                                                           agcagGTGAAGTACGGTGTTAGTCGATCCACCCATcgcg                          >  1:273990/1‑39 (MQ=255)
                                                             |                                                            
GGGAAAGCTTATCGATATCACTCATGGTGAAGTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAGTCGATCCACCCATCGCGATATCCAGCGTCATGGCGTTTTCA  >  minE/2444220‑2444341

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: