Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,444,290 T→A 23.3% V282V (GTA→GTT ilvD ← dihydroxyacid dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,444,2900TA23.3% 224.4 / 65.1 133V282V (GTA→GTTilvDdihydroxyacid dehydratase
Reads supporting (aligned to +/- strand):  ref base T (43/59);  new base A (9/22);  total (52/81)
Fisher's exact test for biased strand distribution p-value = 2.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01

Only 100 of 137 total aligned reads displayed.

TATCGATATCACTCATGGTGAAGTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAGTCGATCCACCCATCGCGATATCCAGCGTCATGGCGTTTTCAAACGCCGCCTTACTGGC  >  minE/2444229‑2444358
                                                             |                                                                    
tATCGATATCACTCATGGTGAAGTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGgtgt                                                               <  1:110718/69‑1 (MQ=255)
tATCGATATCACTCATGGTGAAGTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGgtgt                                                               <  1:169910/69‑1 (MQ=255)
tATCGATATCACTCATGGTGAAGTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGgtgt                                                               <  1:34075/69‑1 (MQ=255)
tATCGATATCACTCATGGTGAAGTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGgtgt                                                               <  1:838515/69‑1 (MQ=255)
tATCGATATCACTCATGGTGAAGTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGgtgt                                                               <  1:666290/69‑1 (MQ=255)
 aTCGATATCACTCATGGTGAAGTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGtt                                                              >  1:3080372/1‑69 (MQ=255)
 aTCGATATCACTCATGGTGAAGTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGtt                                                              >  1:582587/1‑69 (MQ=255)
      tatCACTCATGGTGAAGTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAGTCg                                                         <  1:40055/69‑1 (MQ=255)
      tatCACTCATGGTGAAGTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAGTCg                                                         <  1:2250569/69‑1 (MQ=255)
      tatCACTCATGGTGAAGTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAGTCg                                                         <  1:3313666/69‑1 (MQ=255)
      tatCACTCATGGTGAAGTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAGTCg                                                         <  1:542765/69‑1 (MQ=255)
      tatCACTCATGGTGAAGTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAGTCg                                                         <  1:1052933/69‑1 (MQ=255)
       atCACTCATGGTGAAGTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAGTCGa                                                        <  1:1250277/69‑1 (MQ=255)
       atCACTCATGGTGAAGTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAGTCGa                                                        <  1:870917/69‑1 (MQ=255)
       atCACTCATGGTGAAGTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAGTCGa                                                        <  1:1145973/69‑1 (MQ=255)
       atCACTCATGGTGAAGTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAGTCGa                                                        <  1:2282680/69‑1 (MQ=255)
       atCACTCATGGTGAAGTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAGTCGa                                                        <  1:1120138/69‑1 (MQ=255)
       atCACTCATGGTGAAGTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAGTCGa                                                        <  1:3006777/69‑1 (MQ=255)
        tCACTCATGGTGAAGTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAGTCGa                                                        <  1:3095298/68‑1 (MQ=255)
        tCACTCATGGTGAAGTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAGTCGa                                                        <  1:2626097/68‑1 (MQ=255)
          aCTCATGGTGAAGTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAg                                                            >  1:1342026/1‑62 (MQ=255)
          aCTCATGGTGAAGTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAg                                                            >  1:2373600/1‑62 (MQ=255)
          aCTCATGGTGAAGTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAg                                                            >  1:2519358/1‑62 (MQ=255)
          aCTCATGGTGAAGTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAg                                                            >  1:2487881/1‑62 (MQ=255)
          aCTCATGGTGAAGTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAGTCGATcc                                                     >  1:92115/1‑69 (MQ=255)
          aCTCATGGTGAAGTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAGTCGATcc                                                     >  1:2276012/1‑69 (MQ=255)
          aCTCATGGTGAAGTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAGTCGATcc                                                     >  1:2696309/1‑69 (MQ=255)
          aCTCATGGTGAAGTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAGTCGATcc                                                     >  1:908091/1‑69 (MQ=255)
          aCTCATGGTGAAGTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAGTCGATcc                                                     >  1:975462/1‑69 (MQ=255)
          aCTCATGGTGAAGTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAGTCGATcc                                                     >  1:613927/1‑69 (MQ=255)
          aCTCATGGTGAAGTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAGTCGATcc                                                     >  1:1067960/1‑69 (MQ=255)
          aCTCATGGTGAAGTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAGTCGATcc                                                     >  1:1791657/1‑69 (MQ=255)
          aCTCATGGTGAAGTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAGTCGATcc                                                     >  1:1645072/1‑69 (MQ=255)
          aCTCATGGTGAAGTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAGTCGATcc                                                     >  1:1587010/1‑69 (MQ=255)
          aCTCATGGTGAAGTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAGTCGATcc                                                     >  1:1960202/1‑69 (MQ=255)
          aCTCATGGTGAAGTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAGTCGATc                                                      >  1:2887725/1‑68 (MQ=255)
            tCATGGTGAAGTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAGTCGATCCa                                                    <  1:476564/68‑1 (MQ=255)
            tCATGGTGAAGTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAGTCGATCCa                                                    <  1:365171/68‑1 (MQ=255)
                 gTGAAGTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAGTCGATCCACCCa                                                <  1:491877/67‑1 (MQ=255)
                 gTGAAGTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAGTCGATCCACCCa                                                <  1:445741/67‑1 (MQ=255)
                 gTGAAGTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAGTCGATCCACCCa                                                <  1:795484/67‑1 (MQ=255)
                   gAAGTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAGTCGATCCACCCATcgc                                            <  1:3133795/69‑1 (MQ=255)
                    aaGTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAGTCGATCCACCCATcgc                                            >  1:3190702/1‑68 (MQ=255)
                    aaGTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAGTCGATCCACCCATcgc                                            >  1:1387033/1‑68 (MQ=255)
                    aaGTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAGTCGATCCACCCATcgc                                            >  1:2553573/1‑68 (MQ=255)
                    aaGTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAGTCGATCCACCCATcgc                                            >  1:2799613/1‑68 (MQ=255)
                    aaGTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAGTCGATCCACCCATcgc                                            >  1:3330771/1‑68 (MQ=255)
                      gTCGATTTCCGCTTCCTGCGCCGCCGCCAGGAGGTGAAGTACGGTGTTAGTCGATCCACCCATCGCGat                                         <  1:877466/69‑1 (MQ=255)
                      gTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAGTCGATCCACCCATCGCGat                                         >  1:1531284/1‑69 (MQ=255)
                      gTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAGTCGATCCACCCATCGCGat                                         <  1:3167431/69‑1 (MQ=255)
                      gTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAGTCGATCCACCCATCGCGat                                         <  1:3132633/69‑1 (MQ=255)
                      gTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAGTCGATCCACCCATCGCGat                                         <  1:905273/69‑1 (MQ=255)
                      gTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAGTCGATCCACCCATCGCGat                                         <  1:3280427/69‑1 (MQ=255)
                      gTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAGTCGATCCACCCATCGCGat                                         <  1:1856942/69‑1 (MQ=255)
                      gTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAGTCGATCCACCCATCGCGat                                         <  1:1217358/69‑1 (MQ=255)
                      gTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAGTCGATCCACCCATCGCGat                                         >  1:2007812/1‑69 (MQ=255)
                      gTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAGTCGATCCACCCATCGCGat                                         <  1:248414/69‑1 (MQ=255)
                      gTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAGTCGATCCACCCATCGCGat                                         >  1:58100/1‑69 (MQ=255)
                      gTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAGTCGATCCACCCATCGCGat                                         <  1:259324/69‑1 (MQ=255)
                      gTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAGTCGATCCACCCATCGCGat                                         >  1:25295/1‑69 (MQ=255)
                      gTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAGTCGATCCACCCATCGCGat                                         <  1:1952884/69‑1 (MQ=255)
                         gATTTCCGCTTCCTGCGCCGCCGCCAGTAGGTGAAGAACGgtgt                                                               >  1:425013/1‑44 (MQ=38)
                         gATTTCCGCTTCCTGCGCCGCCGCCAGTAGGTGAAGAACGgtgt                                                               >  1:2192215/1‑44 (MQ=38)
                         gATTTCCGCTTCCTGCGCCGCCGCCAGTAGGTGAAGAACGgtgt                                                               >  1:672757/1‑44 (MQ=38)
                         gATTTCCGCTTCCTGCGCCGCCGCCAGTAGGTGAAGAACGgtgt                                                               >  1:3188165/1‑44 (MQ=38)
                         gATTTCCGCTTCCTGCGCCGCCGCCAGTAGGTGAAGAACGgtgt                                                               >  1:2410223/1‑44 (MQ=38)
                         gATTTCCGCTTCCTGCGCCGCCGCCAGTAGGTGAAGAACGgtgt                                                               >  1:3015099/1‑44 (MQ=38)
                         gATTTCCGCTTCCTGCGCCGCCGCCAGTAGGTGAAGAACGgtgt                                                               >  1:286267/1‑44 (MQ=38)
                         gATTTCCGCTTCCTGCGCCGCCGCCAGTAGGTGAAGAACGgtgt                                                               >  1:2872619/1‑44 (MQ=38)
                           tttCCGCTTCCTGCGCCGCCGCCAGTATGTGAAGAACGGTGTTGGt                                                           <  1:216306/46‑1 (MQ=37)
                           tttCCGCTTCCTGCGCCGCCGCCAGTAGGTGAAGAACGGTGTTGGt                                                           <  1:634495/46‑1 (MQ=38)
                           tttCCGCTTCCTGCGCCGCCGCCAGTAGGTGAAGAACGGTGTTGGt                                                           <  1:1132916/46‑1 (MQ=38)
                           tttCCGCTTCCTGCGCCGCCGCCAGTAGGTGAAGAACGGTGTTGGt                                                           <  1:2102525/46‑1 (MQ=38)
                           tttCCGCTTCCTGCGCCGCCGCCAGTAGGTGAAGAACGGTGTTGGt                                                           <  1:1185810/46‑1 (MQ=38)
                           tttCCGCTTCCTGCGCCGCCGCCAGTAGGTGAAGAACGGTGTTGGt                                                           <  1:1990353/46‑1 (MQ=38)
                           tttCCGCTTCCTGCGCCGCCGCCAGTAGGTGAAGAACGGTGTTGGt                                                           <  1:774851/46‑1 (MQ=38)
                           tttCCGCTTCCTGCGCCGCCGCCAGTAGGTGAAGAACGGTGTTGGt                                                           <  1:68881/46‑1 (MQ=38)
                           tttCCGCTTCCTGCGCCGCCGCCAGTAGGTGAAGAACGGTGTTGGt                                                           <  1:1806061/46‑1 (MQ=38)
                           tttCCGCTTCCTGCGCCGCCGCCAGTAGGTGAAGAACGGTGTTGGt                                                           <  1:2533641/46‑1 (MQ=38)
                           tttCCGCTTCCTGCGCCGCCGCCAGTAGGTGAAGAACGGTGTTGGt                                                           <  1:1937471/46‑1 (MQ=38)
                           tttCCGCTTCCTGCGCCGCCGCCAGTAGGTGAAGAACGGTGTTGGt                                                           <  1:3413763/46‑1 (MQ=38)
                           tttCCGCTTCCTGCGCCGCCGCCAGTAGGTGAAGAACGGTGTTGGt                                                           <  1:934388/46‑1 (MQ=38)
                           tttCCGCTTCCTGCGCCGCCGCCAGTAGGTGAAGAACGGTGTTGGt                                                           <  1:1923346/46‑1 (MQ=38)
                           tttCCGCTTCCTGCGCCGCCGCCAGTAGGTGAAGAACGGTGTTGGt                                                           <  1:1519161/46‑1 (MQ=38)
                           tttCCGCTTCCTGCGCCGCAGCCAGTAGGTGAAGAACGGTGTTGGt                                                           <  1:1251074/46‑1 (MQ=25)
                              ccGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAGTCGATCCACCCATCGCGATATCCAGc                                  >  1:2882055/1‑68 (MQ=255)
                                gCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAGTCGATCCACCCATCGCGATATCCAGCGt                                <  1:72056/68‑1 (MQ=255)
                                gCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAGTCGATCCACCCATCGCGATATCCAGCGt                                <  1:208747/68‑1 (MQ=255)
                                    ccTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAGTCGATCCACCCATCGCGATATCCAGCGTCATgg                           >  1:467231/1‑69 (MQ=255)
                                    ccTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAGTCGATCCACCCATCGCGATATCCAGCGTCATgg                           >  1:744583/1‑69 (MQ=255)
                                            gccgccAGCAGGTGAAGTACGGTGTTAGTCGATCCACCCATCGCGATATCCAGCGTCATGGCGTTTTCa                   <  1:671824/69‑1 (MQ=255)
                                            gccgccAGCAGGTGAAGTACGGTGTTAGTCGATCCACCCATCGCGATATCCAGCGTCATGGCGTTTTCa                   <  1:224176/69‑1 (MQ=255)
                                                  agcagGTGAAGTACGGTGTTAGTCGATCCACCCATcgcg                                           >  1:273990/1‑39 (MQ=255)
                                                      ggTGAAGTACGGTGTTAGTCGATCCACCCATCGCGATATCCAGCGTCATGGCGTTTTCAAACGCCGCCt         <  1:2687398/69‑1 (MQ=255)
                                                       gTGAAGTACGGTGTTAGTCGATCCACCCATCGCGATATCCAGCGTCATGGCGTTTTCAAACGCCGCCtt        <  1:1352065/69‑1 (MQ=255)
                                                       gTGAAGTACGGTGTTAGTCGATCCACCCATCGCGATATCCAGCGTCATGGCGTTTTCAAACGCCGACtt        <  1:388833/69‑1 (MQ=255)
                                                         gAAGTACGGTGTTAGTCGATCCACCCATCGCGATATCCAGCGTCATGGCGTTTTCAAACGCCGCCTTAc      >  1:388425/1‑69 (MQ=255)
                                                             tACGGTGTTAGTCGATCCACCCATCGCGATATCCAGCGTCATGGCGTTTTCAAACGCCGCCTTACTGGc  <  1:1052730/69‑1 (MQ=255)
                                                             tACGGTGTTAGTCGATCCACCCATCGCGATATCCAGCGTCATGGCGTTTTCAAACGCCGCCTTACTGGc  <  1:27947/69‑1 (MQ=255)
                                                             tACGGTGTTAGTCGATCCACCCATCGCGATATCCAGCGTCATGGCGTTTTCAAACGCCGCCTTACTGGc  <  1:3087265/69‑1 (MQ=255)
                                                             |                                                                    
TATCGATATCACTCATGGTGAAGTCGATTTCCGCTTCCTGCGCCGCCGCCAGCAGGTGAAGTACGGTGTTAGTCGATCCACCCATCGCGATATCCAGCGTCATGGCGTTTTCAAACGCCGCCTTACTGGC  >  minE/2444229‑2444358

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: