| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,444,536 | G→A | 47.1% | T200T (ACC→ACT) | ilvD ← | dihydroxyacid dehydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,444,536 | 0 | G | A | 47.1% | 9.1 / 73.6 | 87 | T200T (ACC→ACT) | ilvD | dihydroxyacid dehydratase |
| Reads supporting (aligned to +/- strand): ref base G (29/17); new base A (3/38); total (32/55) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.56e-08 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.67e-01 | |||||||||||
CGAGCCGTTGCCCGGCTGCGACAGGCCCAGCGCTTCGGTCAGGCAGTTCATTGAGTTAGCGGTAAACATCCCGGAGCAGGAACCGCAGGTCGGACACGCGGAACGTTCAACCTGATCGCTC > minE/2444476‑2444596 | cGAGCCGTTGCCCGGCTGCGACAGGCCCAGCGCTTCGGTCAGGCAGTTCATTGAGTTAGCGGTAAACAt < 1:793370/69‑1 (MQ=255)cGAGCCGTTGCCCGGCTGCGACAGGCCCAGCGCTTCGGTCAGGCAGTTCATTGAGTTAGCGGTAAACAt < 1:2794870/69‑1 (MQ=255)cGAGCCGTTGCCCGGCTGCGACAGGCCCAGCGCTTCGGTCAGGCAGTTCATTGAGTTAGCGGTAAACAt < 1:2655869/69‑1 (MQ=255)cGAGCCGTTGCCCGGCTGCGACAGGCCCAGCGCTTCGGTCAGGCAGTTCATTGAGTTAGCGGTAAACAt < 1:2584047/69‑1 (MQ=255)cGAGCCGTTGCCCGGCTGCGACAGGCCCAGCGCTTCGGTCAGGCAGTTCATTGAGTTAGCGGTAAACAt < 1:1640115/69‑1 (MQ=255)cGAGCCGTTGCCCGGCTGCGACAGG‑CCAGCGCTTCGGTCAGGCAGTTCATTGAGTTAGCGGTAAACAt < 1:514612/68‑1 (MQ=255) gAGCCGTTGCCCGGCTGCGACAGGCCCAGCGCTTCGGTCAGGCAGTTCATTGAGTTAGCGGTAAACAt < 1:1503449/68‑1 (MQ=255) ccGTTGCCCGGCTGCGACAGGCCCAGCGCTTCGGTCAGGCAGTTCATTGAGTTAGCGGTATACATCCCg < 1:1617255/69‑1 (MQ=255) cGTTGCCCGGCTGCGACAGGCCCAGCGCTTCGGTCAGGCAGTTCATTGAGTTAGCGGTAAACATCCCgg < 1:1166431/69‑1 (MQ=255) cGTTGCCCGGCTGCGACAGGCCCAGCGCTTCGGTCAGGCAGTTCATTGAGTTAGCGGTAAACATCCCgg < 1:96716/69‑1 (MQ=255) cGTTGCCCGGCTGCGACAGGCCCAGCGCTTCGGTCAGGCAGTTCATTGAGTTAGCGGTAAACATCCCgg < 1:768367/69‑1 (MQ=255) cGTTGCCCGGCTGCGACAGGCCCAGCGCTTCGGTCAGGCAGTTCATTGAGTTAGCGGTAAACATCCCgg < 1:70717/69‑1 (MQ=255) cGTTGCCCGGCTGCGACAGGCCCAGCGCTTCGGTCAGGCAGTTCATTGAGTTAGCGGTAAACATCCCgg < 1:3116070/69‑1 (MQ=255) cGTTGCCCGGCTGCGACAGGCCCAGCGCTTCGGTCAGGCAGTTCATTGAGTTAGCGGTAAACATCCCgg < 1:2987508/69‑1 (MQ=255) cGTTGCCCGGCTGCGACAGGCCCAGCGCTTCGGTCAGGCAGTTCATTGAGTTAGCGGTAAACATCCCgg < 1:2267369/69‑1 (MQ=255) gTTGCCCGGCTGCGACAGGCCCAGCGCTTCGGTCAGGCAGTTCATTGAGTTAGCGGTAAACATCCCgg < 1:2955690/68‑1 (MQ=255) gTTGCCCGGCTGCGACAGGCCCAGCGCTTCGGTCAGGCAGTTCATTGAGTTAGCGGTAAACATCCCgg < 1:2034267/68‑1 (MQ=255) gCGACAGGCCCAGCGCTTCGGTCAGGCAGTTCATTGAGTTAGCAGTAAACATCCCGGAGCAGGAACCGc > 1:3280021/1‑69 (MQ=255) gCGACAGGCCCAGCGCTTCGGTCAGGCAGTTCATTGAGTTAGCAGTAAACATCCCGGAGCAGGAACCGc > 1:1934319/1‑69 (MQ=255) gCGACAGGCCCAGCGCTTCGGTCAGGCAGTTCATTGAGTTAGCAGTAAACATCCCGGAGCAGGAACCGc > 1:821362/1‑69 (MQ=255) cgcTTCGGTCAGGCAGTTCATTGAGTTATCGGTAAACATCCCGGa > 1:2135675/1‑45 (MQ=255) cgcTTCGGTCAGGCAGTTCATTGAGTTAGCGGTAAACATCCCGGAGCAGGAACCGCAGGTCGGacac > 1:2471608/1‑67 (MQ=255) cgcTTCGGTCAGGCAGTTCATTGAGTTAGCGGTAAACATCCCGGAGCAGGAACCGCAGGTCGGACAcg > 1:3371579/1‑68 (MQ=255) cgcTTCGGTCAGGCAGTTCATTGAGTTAGCGGTAAACATCCCGGAGCAGGAACCGCAGGTCGGACAcg > 1:124405/1‑68 (MQ=255) cgcTTCGGTCAGGCAGTTCATTGAGTTAGCGGTAAACATCCCGGAGCAGGAACCGCAGGTCGGACAcg > 1:3311570/1‑68 (MQ=255) cgcTTCGGTCAGGCAGTTCATTGAGTTAGCGGTAAACATCCCGGAGCAGGAACCGCAGGTCGGACAcg > 1:316725/1‑68 (MQ=255) cgcTTCGGTCAGGCAGTTCATTGAGTTAGCGGTAAACATCCCGGAGCAGGAACCGCAGGTCGGACAcg > 1:3299848/1‑68 (MQ=255) cAGTTCATTGAGTTAGCGGTAAACATCCCGGAGCAGGAACCGCAGGTCGGACACGCGGAACGTTCaa > 1:1484182/1‑67 (MQ=255) cAGTTCATTGAGTTAGCAGTAAACATCCCGGAGCAGGAACCGCAGGTTGGGCAGTCGGATCGTTCAAcc < 1:3067542/69‑1 (MQ=255) cAGTTCATTGAGTTAGCAGTAAACATCCCGGAGCAGGAACCGCAGGTTGGGCAGGCGGATCGTTCAAcc < 1:1543021/69‑1 (MQ=255) cAGTTCATTGAGTTAGCAGTAAACATCCCGGAGCAGGAACCGCAGGTTGGGCAGGCGGATCGTTCAAcc < 1:3389149/69‑1 (MQ=255) cAGTTCATTGAGTTAGCAGTAAACATCCCGGAGCAGGAACCGCAGGTTGGGCAGGCGGATCGTTCAAcc < 1:3254161/69‑1 (MQ=255) cAGTTCATTGAGTTAGCAGTAAACATCCCGGAGCAGGAACCGCAGGTTGGGCAGGCGGATCGTTCAAcc < 1:454886/69‑1 (MQ=255) cAGTTCATTGAGTTAGCAGTAAACATCCCGGAGCAGGAACCGCAGGTTGGGCAGGCGGATCGTTCAAcc < 1:537603/69‑1 (MQ=255) cAGTTCATTGAGTTAGCAGTAAACATCCCGGAGCAGGAACCGCAGGTTGGGCAGGCGGATCGTTCAAcc < 1:2875592/69‑1 (MQ=255) cAGTTCATTGAGTTAGCAGTAAACATCCCGGAGCAGGAACCGCAGGTTGGGCAGGCGGATCGTTCAAcc < 1:2810334/69‑1 (MQ=255) cAGTTCATTGAGTTAGCAGTAAACATCCCGGAGCAGGAACCGCAGGTTGGGCAGGCGGATCGTTCAAcc < 1:2547019/69‑1 (MQ=255) cAGTTCATTGAGTTAGCAGTAAACATCCCGGAGCAGGAACCGCAGGTTGGGCAGGCGGATCGTTCAAcc < 1:1763040/69‑1 (MQ=255) cAGTTCATTGAGTTAGCAGTAAACATCCCGGAGCAGGAACCGCAGGTTGGGCAGGCGGATCGTTCAAcc < 1:2267242/69‑1 (MQ=255) cAGTTCATTGAGTTAGCAGTAAACATCCCGGAGCAGGAACCGCAGGTTGGGCAGGCGGATCGTTCAAcc < 1:811390/69‑1 (MQ=255) cAGTTCATTGAGTTAGCAGTAAACATCCCGGAGCAGGAACCGCAGGTTGGGCAGGCGGATCGTTCAAcc < 1:2048398/69‑1 (MQ=255) aGTTCATTGAGTTAGCAGTAAACATCCCGGAGCAGGAACCGCAGGTTGGGCAGGCGGATCGTTCAAcc < 1:3030078/68‑1 (MQ=255) tCATTGAGTTAGCGGTAAACATCCCGGAGCAGGAACCGCAGGTCGGACACGCGGAACGTTCAACCTGAt > 1:3214262/1‑69 (MQ=255) tCATTGAGTTAGCGGTAAACATCCCGGAGCAGGAACCGCAGGTCGGACACGCGGAACGTTCAACCTGAt > 1:916476/1‑69 (MQ=255) tCATTGAGTTAGCGGTAAACATCCCGGAGCAGGAACCGCAGGTCGGACACGCGGAACGTTCAACCTGAt > 1:2831960/1‑69 (MQ=255) cATTGAGTTAGCGGTAAACATCCCGGAGCAGGAACCGCAGGTCGGACACGCGGAACGTTc > 1:3094782/1‑60 (MQ=255) cATTGAGTTAGCGGTAAACATCCCGGAGCAGGAACCGCAGGTCGGACACGCGGAACGTTCa > 1:2412417/1‑61 (MQ=255) cATTGAGTTAGCGGTAAACATCCCGGAGCAGGAACCGCAGGTCGGACACGCGGAACGTTCa > 1:150640/1‑61 (MQ=255) cATTGAGTTAGCGGTAAACATCCCGGAGCAGGAACCGCAGGTCGGACACGCGGAACGTTCa > 1:1501089/1‑61 (MQ=255) cATTGAGTTAGCGGTAAACATCCCGGAGCAGGAACCGCAGGTCGGACACGCGGAACGTTCa > 1:2062197/1‑61 (MQ=255) cATTGAGTTAGCGGTAAACATCCCGGAGCAGGAACCGCAGGTCGGACACGCGGAACGTTCa > 1:2197915/1‑61 (MQ=255) cATTGAGTTAGCGGTAAACATCCCGGAGCAGGAACCGCAGGTCGGACACGCGGAACGTTCa > 1:2953289/1‑61 (MQ=255) cATTGAGTTAGCGGTAAACATCCCGGAGCAGGAACCGCAGGTCGGACACGCGGAACGTTCa > 1:2213707/1‑61 (MQ=255) cATTGAGTTAGCGGTAAACATCCCGGAGCAGGAACCGCAGGTCGGACACGCGGAACGTTCa > 1:492634/1‑61 (MQ=255) cATTGAGTTAGCGGTAAACATCCCGGAGCAGGAACCGCAGGTCGGACACGCGGAACGTTCa > 1:1286140/1‑61 (MQ=255) cATTGAGTTAGCGGTAAACATCCCGGAGCAGGAACCGCAGGTCGGACACGCGGAACGTTCa > 1:1284510/1‑61 (MQ=255) cATTGAGTTAGCGGTAAACATCCCGGAGCAGGAACCGCAGGTCGGACACGCGGAACGTTCa > 1:2291706/1‑61 (MQ=255) cATTGAGTTAGCGGTAAACATCCCGGAGCAGGAACCGCAGGTCGGACACGCGGAACGTTCa > 1:583522/1‑61 (MQ=255) cATTGAGTTAGCGGTAAACATCCCGGAGCAGGAACCGCAGGTCGGACACGCGGAACGTTCa > 1:2697501/1‑61 (MQ=255) cATTGAGTTAGCGGTAAACATCCCGGAGCAGGAACCGCAGGTCGGACACGCGGAACGTTCa > 1:1438589/1‑61 (MQ=255) cATTGAGTTAGCGGTAAACATCCCGGAGCAGGAACCGAAGGTCGGACACGCGGAACGTTCa > 1:3283842/1‑61 (MQ=255) cATTGAGTTAGCAGTAAACATCCCGGAGCAGGAACCGCAGGTTGGGCAGGCGGATCGTTCAAcc < 1:159038/64‑1 (MQ=255) tGAGTTAGCGGTAAACATCCCGGAGCAGGAACCGCAGGTCGGACACGCGGAACGTTCAACCTGATCGct > 1:3041085/1‑69 (MQ=255) tGAGTTAGCGGTAAACATCCCGGAGCAGGAACCGCAGGTCGGACACGCGGAACGTTCAACCTGATCGct > 1:3420366/1‑69 (MQ=255) gAGTTAGCAGTAAACATCCCGGAGCAGGAACCGCAGGTTGGGCAGGCGGATGGTTCAACCTGAGCGctc < 1:681970/69‑1 (MQ=255) gAGTTAGCAGTAAACATCCCGGAGCAGGAACCGCAGGTTGGGCAGGCGGATCGTTCAACCTGAGCGctc < 1:1143863/69‑1 (MQ=255) gAGTTAGCAGTAAACATCCCGGAGCAGGAACCGCAGGTTGGGCAGGCGGATCGTTCAACCTGAGCGctc < 1:1327774/69‑1 (MQ=255) gAGTTAGCAGTAAACATCCCGGAGCAGGAACCGCAGGTTGGGCAGGCGGATCGTTCAACCTGAGCGctc < 1:1727130/69‑1 (MQ=255) gAGTTAGCAGTAAACATCCCGGAGCAGGAACCGCAGGTTGGGCAGGCGGATCGTTCAACCTGAGCGctc < 1:855580/69‑1 (MQ=255) gAGTTAGCAGTAAACATCCCGGAGCAGGAACCGCAGGTTGGGCAGGCGGATCGTTCAACCTGAGCGctc < 1:850686/69‑1 (MQ=255) gAGTTAGCAGTAAACATCCCGGAGCAGGAACCGCAGGTTGGGCAGGCGGATCGTTCAACCTGAGCGctc < 1:1986085/69‑1 (MQ=255) gAGTTAGCAGTAAACATCCCGGAGCAGGAACCGCAGGTTGGGCAGGCGGATCGTTCAACCTGAGCGctc < 1:2010370/69‑1 (MQ=255) gAGTTAGCAGTAAACATCCCGGAGCAGGAACCGCAGGTTGGGCAGGCGGATCGTTCAACCTGAGCGctc < 1:1180875/69‑1 (MQ=255) gAGTTAGCAGTAAACATCCCGGAGCAGGAACCGCAGGTTGGGCAGGCGGATCGTTCAACCTGAGCGctc < 1:2151698/69‑1 (MQ=255) gAGTTAGCAGTAAACATCCCGGAGCAGGAACCGCAGGTTGGGCAGGCGGATCGTTCAACCTGAGCGctc < 1:732413/69‑1 (MQ=255) gAGTTAGCAGTAAACATCCCGGAGCAGGAACCGCAGGTTGGGCAGGCGGATCGTTCAACCTGAGCGctc < 1:1486262/69‑1 (MQ=255) gAGTTAGCAGTAAACATCCCGGAGCAGGAACCGCAGGTTGGGCAGGCGGATCGTTCAACCTGAGCGctc < 1:662026/69‑1 (MQ=255) gAGTTAGCAGTAAACATCCCGGAGCAGGAACCGCAGGTTGGGCAGGCGGATCGTTCAACCTGAGCGctc < 1:577737/69‑1 (MQ=255) gAGTTAGCAGTAAACATCCCGGAGCAGGAACCGCAGGTTGGGCAGGCGGATCGTTCAACCTGAGCGctc < 1:2906666/69‑1 (MQ=255) gAGTTAGCAGTAAACATCCCGGAGCAGGAACCGCAGGTTGGGCAGGCGGATCGTTCAACCTGAGCGctc < 1:2944867/69‑1 (MQ=255) gAGTTAGCAGTAAACATCCCGGAGCAGGAACCGCAGGTTGGGCAGGCGGATCGTTCAACCTGAGCGctc < 1:486574/69‑1 (MQ=255) gAGTTAGCAGTAAACATCCCGGAGCAGGAACCGCAGGTTGGGCAGGCGGATCGTTCAACCTGAGCGctc < 1:2952492/69‑1 (MQ=255) gAGTTAGCAGTAAACATCCCGGAGCAGGAACCGCAGGTTGGGCAGGCGGATCGTTCAACCTGAGCGctc < 1:3418929/69‑1 (MQ=255) gAGTTAGCAGTAAACATCCCGGAGCAGGAACCGCAGGTTGGGCAGGCGGATCGTTCAACCTGAGCGctc < 1:3408525/69‑1 (MQ=255) gAGTTAGCAGTAAACATCCCGGAGCAGGAACCGCAGGTTGGGCAGGCGGATCGTTCAACCTGAGCGctc < 1:1331613/69‑1 (MQ=255) aGTTAGCAGTAAACATCCCGGAGCAGGAACCGCAGGTTTGGCAGGCGGATCGTTCAACCTGAGCGctc < 1:2884720/68‑1 (MQ=255) gTTAGCAGTAAACATCCCGGAGCAGGAACCGCAGGTTGGGCAGGCGGATCGTTCAAcc < 1:1382845/58‑1 (MQ=255) | CGAGCCGTTGCCCGGCTGCGACAGGCCCAGCGCTTCGGTCAGGCAGTTCATTGAGTTAGCGGTAAACATCCCGGAGCAGGAACCGCAGGTCGGACACGCGGAACGTTCAACCTGATCGCTC > minE/2444476‑2444596 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |