| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,444,608 | T→A | 40.0% | V176V (GTA→GTT) | ilvD ← | dihydroxyacid dehydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,444,608 | 0 | T | A | 40.0% | 12.3 / 25.3 | 30 | V176V (GTA→GTT) | ilvD | dihydroxyacid dehydratase |
| Reads supporting (aligned to +/- strand): ref base T (15/3); new base A (5/7); total (20/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.50e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.91e-01 | |||||||||||
CCGGAGCAGGAACCGCAGGTCGGACACGCGGAACGTTCAACCTGATCGCTCTGGGAGTCAGATACTTTCGGGTCTGCGCCCTGGATCATCGCATCAACCAGATCGAGCTTGATGATCTGATCGGAAAGTTT > minE/2444546‑2444676 | ccGGAGCAGGAACCGCAGGTCGGACACGCGGAACGTTCAACCTGATCGCTCTGGGAGTCAGATACTTTc > 1:902647/1‑69 (MQ=255)ccGGAGCAGGAACCGCAGGTCGGACACGCGGAACGTTCAACCTGATCGCTCTGGGAGTCAGATACTTTc > 1:3282669/1‑69 (MQ=255)ccGGAGCAGGAACCGCAGGTCGGACACGCGGAACGTTCAACCTGATCGCTCTGGGAGTCAGATACTTTc > 1:1682827/1‑69 (MQ=255) ggAGCAGGAACCGCAGGTTGGGCAGGCGGATCGTTCAACCTGAGCGCTCTGGGAGTCAGAAACTTTCgg > 1:1245394/1‑69 (MQ=255) ggAGCAGGAACCGCAGGTTGGGCAGGCGGATCGTTCAACCTGAGCGCTCTGGGAGTCAGAAACTTTCgg > 1:3176722/1‑69 (MQ=255) ggAGCAGGAACCGCAGGTTGGGCAGGCGGATCGTTCAACCTGAGCGCTCTGGGAGTCAGAAACTTTCgg > 1:271683/1‑69 (MQ=255) ggAGCAGGAACCGCAGGTTGGGCAGGCGGATCGTTCAACCTGAGCGCTCTGGGAGTCAGAAACTTTCgg > 1:2416727/1‑69 (MQ=255) ggAGCAGGAACCGCAGGTTGGGCAGGCGGATCGTTCAACCTGAGCGCTCTGGGAGTCAGAAACTTTCgg > 1:2265696/1‑69 (MQ=255) tCGGACACGCGGAACGTTCAACCTGATCGCTCTGGGAGTCAGATACTTTCGGGTc > 1:1452729/1‑55 (MQ=255) tCGGACACGCGGAACGTTCAACCTGATCGCTCTGGGAGTCAGATACTTTCGGGTCTGCGCCCTGGatca > 1:1731877/1‑69 (MQ=255) tCGGACACGCGGAACGTTCAACCTGATCGCTCTGGGAGTCAGATACTTTCGGGTCTGCGCCCTGGatca > 1:43439/1‑69 (MQ=255) tCGGACACGCGGAACGTTCAACCTGATCGCTCTGGGAGTCAGATACTTTCGGGTCTGCGCCCTGGatca > 1:3399657/1‑69 (MQ=255) tCGGACACGCGGAACGTTCAACCTGATCGCTCTGGGAGTCAGATACTTTCGGGTCTGCGCCCTGGatca > 1:2602619/1‑69 (MQ=255) tCGGACACGCGGAACGTTCAACCTGATCGCTCTGGGAGTCAGATACTTTCGGGTCTGCGCCCTGGatca > 1:2553748/1‑69 (MQ=255) cGCTCTGGGAGTCAGATACTTTCTGGTCTGCGCCCTGGATCATCGCATCAACCAGATCGAGCTtgatg < 1:2282724/68‑1 (MQ=255) cGCTCTGGGAGTCAGAAACTTTCGGGTCTGCGCCCTGGATCATCGCATCAACCAGATCGAGCTtaatg < 1:122517/68‑1 (MQ=255) cGCTCTGGGAGTCAGAAACTTTCGGGTCTGCGCCCTGGATCATCGCATCAACCAGATCGAGCTtaatg < 1:879034/68‑1 (MQ=255) cGCTCTGGGAGTCAGAAACTTTCGGGTCTGCGCCCTGGATCATCGCATCAACCAGATCGAGCTtaatg < 1:836442/68‑1 (MQ=255) cGCTCTGGGAGTCAGAAACTTTCGGGTCTGCGCCCTGGATCATCGCATCAACCAGATCGAGCTtaatg < 1:1445280/68‑1 (MQ=255) cGCTCTGGGAGTCAGAAACTTTCGGGTCTGCGCCCTGGATCATCGCATCAACCAGATCGAGCTtaatg < 1:241124/68‑1 (MQ=255) cGCTCTGGGAGTCAGAAACTTTCGGGTCTGCGCCCTGGATCATCGCATCAACCAGATCGAGCTtaatg < 1:2063240/68‑1 (MQ=255) cGCTCTGGGAGTCAGAAACTTTCGGGTCTGCGCCCTGGATCATCGCATCAACCAGATCGAGCTtaatg < 1:1863988/68‑1 (MQ=255) gCTCTGGGAGTCAGATACTTTCGGGTCTGCGCCCTTGATCATCGCATCAACCAGATCGAGCtt > 1:2234391/1‑63 (MQ=255) gCTCTGGGAGTCAGATACTTTCGGGTCTGCGCCCTGGATCATCGCATCAACCAGATc > 1:1516307/1‑57 (MQ=255) gCTCTGGGAGTCAGATACTTTCGGGTCTGCGCCCTGGATCATCGCATCAACCAGATCGAGCtt > 1:1435002/1‑63 (MQ=255) gCTCTGGGAGTCAGATACTTTCGGGTCTGCGCCCTGGATCATCGCATCAACCAGATCGAGCtt > 1:1275628/1‑63 (MQ=255) gCTCTGGGAGTCAGATACTTTCGGGTCTGCGCCCTGGATCATCGCATCAACCAGATCGAGCtt > 1:1765266/1‑63 (MQ=255) gCTCTGGGAGTCAGATACTTTCGGGTCTGCGCCCTGGATCATCGCATCAACCAGATCGAGCtt > 1:884290/1‑63 (MQ=255) gTCAGATACTTTCGGGTCTGCGCCCTGGATCATCGCATCAACCAGATCGAGCTTGATGATCTGATCGGa < 1:2475833/69‑1 (MQ=255) gTCAGATACTTTCGGGTCTGCGCCCTGGATCATCGCATCAACCAGATCGAGCTTGATGATCTGATCGGa < 1:2115481/69‑1 (MQ=255) tACTTTCGGGTCTGCGCCCTGGATCATCGCATCAACCAGATCGAGCTTGATGATCTGATCGGAAAGttt > 1:2339127/1‑69 (MQ=255) tACTTTCGGGTCTGCGCCCTGGATCATCGCATCAACCAGATCGAGCTTGATGATCTGATCGGAAAGttt > 1:1463482/1‑69 (MQ=255) tACTTTCGGGTCTGCGCCCTGGATCATCGCATCAACCAGATCGAGCTTGATGATCTGATCGGAAAGttt > 1:2258561/1‑69 (MQ=255) tACTTTCGGGTCTGCGCCCTGGATCATCGCATCAACCAGATCGAGCTTGATGATCTGATCGGAAAGttt > 1:457697/1‑69 (MQ=255) | CCGGAGCAGGAACCGCAGGTCGGACACGCGGAACGTTCAACCTGATCGCTCTGGGAGTCAGATACTTTCGGGTCTGCGCCCTGGATCATCGCATCAACCAGATCGAGCTTGATGATCTGATCGGAAAGTTT > minE/2444546‑2444676 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |