| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,447,263 | A→G | 48.1% | pseudogene (793/1647 nt) | ilvG ← | Mutation in protein compared to W3110: p.Glu327_Ilefs*223 Restoration of IlvG found in MG1655 (548 amino acids), fusion of N‑ter and C‑ter fragments ECK3760:JW3740+JW3741:b4488; large subunit of acetolactate synthase II |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,447,263 | 0 | A | G | 48.1% | ‑0.9 / 70.6 | 79 | pseudogene (793/1647 nt) | ilvG | Mutation in protein compared to W3110: p.Glu327_Ilefs*223 Restoration of IlvG found in MG1655 (548 amino acids), fusion of N‑ter and C‑ter fragments ECK3760:JW3740+JW3741:b4488; large subunit of acetolactate synthase II |
| Reads supporting (aligned to +/- strand): ref base A (22/19); new base G (25/13); total (47/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.60e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.10e-01 | |||||||||||
CGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTA > minE/2447203‑2447329 | cGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACt > 1:1301261/1‑69 (MQ=255)cGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACt > 1:3180236/1‑69 (MQ=255) gtgtTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGc > 1:841321/1‑69 (MQ=255) gtgtTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGc < 1:1141188/69‑1 (MQ=255) gtgtTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGc > 1:509229/1‑69 (MQ=255) cAGTTTGCCAGTCACCCGGTCATCAAAACGTGCGCCCGCGGCGATCAGTAGATCGCACTCCTGCActg > 1:3284917/1‑66 (MQ=25) cAGTTTGCCAGTCACCCGGTCATCAAAACGTGCGCCCACGGCGATCAGTAGATCGCACTCCTGCActg > 1:1604107/1‑66 (MQ=37) cAGTTTGCCAGTCACCCGGTCATCAAAACGTGCGCCCACGGCGATCAGTAGATCGCACTCCTGCActg > 1:2354101/1‑66 (MQ=37) cAGTTTGCCAGTCACCCGGTCATCAAAACGTGCGCCCACGGCGATCAGTAGATCGCACTCCTGCActg > 1:2371885/1‑66 (MQ=37) cAGTTTGCCAGTCACCCGGTCATCAAAACGTGCGCCCACGGCGATCAGTAGATCGCACTCCTGCActg > 1:2153053/1‑66 (MQ=37) cAGTTTGCCAGTCACCCGGTCATCAAAACGTGCGCCCACGGCGATCAGTAGATCGCACTCCTGCActg > 1:3297696/1‑66 (MQ=37) cAGTTTGCCAGTCACCCGGTCATCAAAACGTGCGCCCACGGCGATCAGTAGATCGCACTCCTGCActg > 1:2077265/1‑66 (MQ=37) cAGTTTGCCAGTCACCCGGTCATCAAAACGTGCGCCCACGGCGATCAGTAGATCGCACTCCTGCActg > 1:3278031/1‑66 (MQ=37) cAGTTTGCCAGTCACCCGGTCATCAAAACGTGCGCCCACGGCGATCAGTAGATCGCACTCCTGCActg > 1:1917465/1‑66 (MQ=37) cAGTTTGCCAGTCACCCGGTCATCAAAACGTGCGCCCACGGCGATCAGTAGATCGCACTCCTGCActg > 1:1731173/1‑66 (MQ=37) cAGTTTGCCAGTCACCCGGTCATCAAAACGTGCGCCCACGGCGATCAGTAGATCGCACTCCTGCActg > 1:3092447/1‑66 (MQ=37) cAGTTTGCCAGTCACCCGGTCATCAAAACGTGCGCCCACGGCGATCAGTAGATCGCACTCCTGCActg > 1:1452978/1‑66 (MQ=37) cAGTTTGCCAGTCACCCGGTCATCAAAACGTGCGCCCACGGCGATCAGTAGATCGCACTCCTGCActg > 1:2726628/1‑66 (MQ=37) cAGTTTGCCAGTCACCCGGTCATCAAAACGTGCGCCCACGGCGATCAGTAGATCGCACTCCTGCActg > 1:628462/1‑66 (MQ=37) cAGTTTGCCAGTCACCCGGTCATCAAAACGTGCGCCCACGGCGATCAGTAGATCGCACTCCTGCActg > 1:759418/1‑66 (MQ=37) cAGTTTGCCAGTCACCCGGTCATCAAAACGTGCGCCCACGGCGATCAGTAGATCGCACTCCTGCActg > 1:1101481/1‑66 (MQ=37) cAGTTTGCCAGTCACCCGGTCATCAAAACGTGCGCCCACGGCGATCAGTAGATCGCACTCCTGCActg > 1:283948/1‑66 (MQ=37) cAGTTTGCCAGTCACCCGGTCATCAAAACGTGCGCCCACGGCGATCAGTAGATCGCACTCCTGCActg > 1:2988825/1‑66 (MQ=37) cAGTTTGCCAGTAACCCGGTCATCAAAACGTGCGCCCACGGCGATCAGTAGATCGCACTCCTGCActg > 1:3426814/1‑66 (MQ=255) tGCCAGTCACCCGGTCATCAAAACGTGCGCCCACGGCGATCAGTAGATCGCACTCCTTCACtgcg < 1:2315502/65‑1 (MQ=25) tGCCAGTCACCCGGTCATCAAAACGTGCGCCCACGGCGATCAGTAGATCGCACTCCTGCACtgcg < 1:1047772/65‑1 (MQ=37) tGCCAGTCACCCGGTCATCAAAACGTGCGCCCACGGCGATCAGTAGATCGCACTCCTGCACtgcg < 1:3225984/65‑1 (MQ=37) tGCCAGTCACCCGGTCATCAAAACGTGCGCCCACGGCGATCAGTAGATCGCACTCCTGCACtgcg < 1:3074879/65‑1 (MQ=37) tGCCAGTCACCCGGTCATCAAAACGTGCGCCCACGGCGATCAGTAGATCGCACTCCTGCACtgcg < 1:2379793/65‑1 (MQ=37) tGCCAGTCACCCGGTCATCAAAACGTGCGCCCACGGCGATCAGTAGATCGCACTCCTGCACtgcg < 1:101782/65‑1 (MQ=37) tGCCAGTCACCCGGTCATCAAAACGTGCGCCCACGGCGATCAGTAGATCGCACTCCTGCACtgcg < 1:2331725/65‑1 (MQ=37) tGCCAGTCACCCGGTCATCAAAACGTGCGCCCACGGCGATCAGTAGATCGCACTCCTGCACtgcg < 1:479703/65‑1 (MQ=37) tGCCAGTCACCCGGTCATCAAAACGTGCGCCCACGGCGATCAGTAGATCGCACTCCTGCACtgcg < 1:179519/65‑1 (MQ=37) tGCCAGTCACCCGGTCATCAAAACGTGCGCCCACGGCGATCAGTAGATCGCACTCCTGCACtgcg < 1:520747/65‑1 (MQ=37) tGCCAGTCACCCGGTCATCAAAACGTGCGCCCACGGCGATCAGTAGATCGCACTCCTGCACtgcg < 1:1528856/65‑1 (MQ=37) tGCCAGTCACCCGGTCATCAAAACGTGCGCCCACGGCGATCAGTAGATCGCACTCCTGCACtgcg < 1:531515/65‑1 (MQ=37) tGCCAGTCACCCGGTCATCAAAACGTGCGCCCACGGCGATCAGTAGATCGCACTCCTGCACtgcg < 1:1051650/65‑1 (MQ=37) cacaCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTcgccgc > 1:764155/1‑69 (MQ=255) cACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTcgccgc > 1:328175/1‑69 (MQ=255) cACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTcgccgc > 1:427607/1‑69 (MQ=255) cACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTcgccgc > 1:539459/1‑69 (MQ=255) cACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTcgccgc > 1:2785814/1‑69 (MQ=255) cACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTcgccgc > 1:1983789/1‑69 (MQ=255) cACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTcgccgc > 1:2886710/1‑69 (MQ=255) cACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTcgccgc > 1:2707859/1‑69 (MQ=255) cACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTcgccgc > 1:1059379/1‑69 (MQ=255) cACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTcgccgc > 1:1363081/1‑69 (MQ=255) cACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTcgccgc > 1:2342289/1‑69 (MQ=255) cACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTcgccgc > 1:2381462/1‑69 (MQ=255) catcaAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGTGAAGTTCGCCGCTTTGGTGc < 1:351597/69‑1 (MQ=255) catcaAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGc < 1:3254901/69‑1 (MQ=255) catcaAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGc < 1:1083168/69‑1 (MQ=255) catcaAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGc < 1:333618/69‑1 (MQ=255) catcaAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGc < 1:1968187/69‑1 (MQ=255) catcaAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGc < 1:3174420/69‑1 (MQ=255) catcaAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGc < 1:3072130/69‑1 (MQ=255) caAAACGTGCGCCCACGGCGATCAGTAGATCGCACTCCTGCACTGCGAAGTTCGCCGCTTTGGTGccg > 1:1483585/1‑67 (MQ=255) caAAACGTGCGCCCACGGCGATCAGTAGATCGCACTCCTGCACTGCGAAGTTCGCCGCTTTGGTGccg > 1:573745/1‑67 (MQ=255) caAAACGTGCGCCCACGGCGATCAGTAGATCGCACTCCTGCACTGCGAAGTTCGCCGCTTTGGTGccg > 1:872374/1‑67 (MQ=255) caAAACGTGCGCCCACGGCGATCAGTAGATCGCACTCCTGCACTGCGAAGTTCGCCGCTTTGGTGccg > 1:2693802/1‑67 (MQ=255) caAAACGTGCGCCCACGGCGATCAGTAGATCGCACTCCTGCACTGCGAAGTTCGCCGCTTTGGTGccg > 1:2844618/1‑67 (MQ=255) caAAACGTGCGCCCAAGGCGATCAGTAGATCGCACTCCTGCACTGCGAAGTTCGCCGCTTTGGTGccg > 1:887406/1‑67 (MQ=255) caAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGtt > 1:1360454/1‑52 (MQ=255) caAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCa > 1:478196/1‑68 (MQ=255) caAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCa > 1:2109375/1‑68 (MQ=255) caAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCa > 1:3241463/1‑68 (MQ=255) caAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCa > 1:2553596/1‑68 (MQ=255) aaaaCGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTcgccgc < 1:695869/57‑1 (MQ=255) aaaaCGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATg < 1:482346/69‑1 (MQ=255) gCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTccc < 1:24943/69‑1 (MQ=255) cGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCa < 1:889996/69‑1 (MQ=255) cGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCa < 1:3051945/69‑1 (MQ=255) cAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGAt > 1:1355157/1‑68 (MQ=255) aGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAg < 1:2155940/69‑1 (MQ=255) gCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAg < 1:49966/68‑1 (MQ=255) cAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTa < 1:1569663/69‑1 (MQ=255) cAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTa < 1:792264/69‑1 (MQ=255) cAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTa < 1:2044694/69‑1 (MQ=255) cAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTa < 1:2075889/69‑1 (MQ=255) | CGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTA > minE/2447203‑2447329 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |