| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,447,486 | G→A | 58.7% | pseudogene (570/1647 nt) | ilvG ← | Mutation in protein compared to W3110: p.Glu327_Ilefs*223 Restoration of IlvG found in MG1655 (548 amino acids), fusion of N‑ter and C‑ter fragments ECK3760:JW3740+JW3741:b4488; large subunit of acetolactate synthase II |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,447,486 | 0 | G | A | 58.7% | 60.0 / 127.5 | 167 | pseudogene (570/1647 nt) | ilvG | Mutation in protein compared to W3110: p.Glu327_Ilefs*223 Restoration of IlvG found in MG1655 (548 amino acids), fusion of N‑ter and C‑ter fragments ECK3760:JW3740+JW3741:b4488; large subunit of acetolactate synthase II |
| Reads supporting (aligned to +/- strand): ref base G (32/37); new base A (41/57); total (73/94) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.35e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.94e-01 | |||||||||||
Only 100 of 180 total aligned reads displayed.
TGCGCCATACCCACGCCACCGCCAACGTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGGCGCGCTTGCTCAACTTCGGCATGTGGGAAAGTCACTTCGTTTTCAACGGTGGTGAAC > minE/2447424‑2447543 | tgcGCCATACCCACGCCACCGCCAACGTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGgcgcg < 1:2120458/67‑1 (MQ=255)tgcGCCATACCCACGCCACCGCCAACGTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGgcgcg < 1:3253499/67‑1 (MQ=255)tgcGCCATACCCACGCCACCGCCAACGTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGgcgcg < 1:141169/67‑1 (MQ=255)tgcGCCATACCCACGCCACCGCCAACGTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGgcgcg < 1:3379596/67‑1 (MQ=255)tgcGCCATACCCACGCCACCGCCAACGTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGgcgcg < 1:1549220/67‑1 (MQ=255)tgcGCCATACCCACGCCACCGCCAACGTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGgcgcg < 1:267757/67‑1 (MQ=255)tgcGCCATACCCACGCCACCGCCAACGTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGgcgcg < 1:274960/67‑1 (MQ=255)tgcGCCATACCCACGCCACCGCCAACGTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGgcgcg < 1:2913539/67‑1 (MQ=255)tgcGCCATACCCACGCCACCGCCAACGTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGGCGCGCt < 1:614257/69‑1 (MQ=255) ccATACCCACGCCACCGCCAACGTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGGCGCGCTTGc > 1:174269/1‑68 (MQ=255) ccATACCCACGCCACCGCCAACGTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGGCGCGCTTGc > 1:910771/1‑68 (MQ=255) ccATACCCACGCCACCGCCAACGTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGGCGCGCTTGc > 1:2131867/1‑68 (MQ=255) ccATACCCACGCCACCGCCAACGTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGGCGCGCTTGc > 1:2811726/1‑68 (MQ=255) ccATACCCACGCCACCGCCAACGTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGGCGCGCTTGc > 1:735690/1‑68 (MQ=255) cATACCCACGCCACCGCCAACGTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGGCGCGCTTGCTc < 1:1821118/69‑1 (MQ=255) cccACGCCACCGCCAACGTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGGCGCGCt > 1:2871849/1‑60 (MQ=255) cccACGCCACCGCCAACGTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGGCGCGCTTGCTCAAc > 1:3306578/1‑68 (MQ=255) cccACGCCACCGCCAACGTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGGCGCGCTTGCTCAACt > 1:3146068/1‑69 (MQ=255) cccACGCCACCGCCAACGTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGGCGCGCTTGCTCAACt > 1:1167764/1‑69 (MQ=255) cccACGCCACCGCCAACGTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGACGCGCTTGCTCGAc > 1:3144788/1‑68 (MQ=255) cccACGCCACCGCCAACGTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGACGCGCTTGCTCGACt < 1:348920/69‑1 (MQ=255) cccACGCCACCGCCAACGTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGACGCGCTTGCTCGACt < 1:1896812/69‑1 (MQ=255) cccACGCCACCGCCAACGTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGACGCGCTTGCTCGACt < 1:2070871/69‑1 (MQ=255) cccACGCCACCGCCAACGTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGACGCGCTTGCTCGACt > 1:40904/1‑69 (MQ=255) cccACGCCACCGCCAACGTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGACGCGCTTGCTCGACt < 1:983304/69‑1 (MQ=255) cccACGCCACCGCCAACGTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGACGCGCTTGCTCGACt > 1:2241332/1‑69 (MQ=255) cccACGCCACCGCCAACGTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGACGCGCTTGCTCGACt < 1:2246697/69‑1 (MQ=255) cccACGCCACCGCCAACGTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGACGCGCTTGCTCGACt > 1:2436468/1‑69 (MQ=255) cccACGCCACCGCCAACGTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGACGCGCTTGCTCGACt < 1:257765/69‑1 (MQ=255) cccACGCCACCGCCAACGTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGACGCGCTTGCTCGACt < 1:278775/69‑1 (MQ=255) cccACGCCACCGCCAACGTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGACGCGCTTGCTCGACt < 1:2958353/69‑1 (MQ=255) cccACGCCACCGCCAACGTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGACGCGCTTGCTCGACt > 1:1620181/1‑69 (MQ=255) cccACGCCACCGCCAACGTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGACGCGCTTGCTCGACt > 1:1313286/1‑69 (MQ=255) cccACGCCACCGCCAACGTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGACGCGCTTGCTCGACt > 1:1296279/1‑69 (MQ=255) cccACGCCACCGCCAACGTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGACGCGCTTGCTCGACt < 1:1289143/69‑1 (MQ=255) cccACGCCACCGCCAACGTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGACGCGCTTGCTCGACt < 1:1223350/69‑1 (MQ=255) cccACGCCACCGCCAACGTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGACGCGCTTGCTCGACt < 1:1486520/69‑1 (MQ=255) cccACGCCACCGCCAACGTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGACGCGCTTGCTCGACt < 1:1117103/69‑1 (MQ=255) cccACGCCACCGCCAACGTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGACGCGCTTGCTCGACt < 1:96522/69‑1 (MQ=255) cccACGCCACCGCCAACGTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGACGCGCTTGCTCGACt > 1:581177/1‑69 (MQ=255) cccACGCCACCGCCAACGTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGACGCGCTTGCTCGACt > 1:1873453/1‑69 (MQ=255) cGCCACCGCCAACGTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGGCGCGCTTGCTCAACTTCgg < 1:3068942/69‑1 (MQ=255) cGCCACCGCCAACGTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGGCGCGCTTGCTCAACTTCgg < 1:1198574/69‑1 (MQ=255) cGCCACCGCCAACGTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGGCGCGCTTGCTCAACTTCgg < 1:1799879/69‑1 (MQ=255) cGCCACCGCCAACGTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGGCGCGCTTGCTCAACTTCgg < 1:1844807/69‑1 (MQ=255) cGCCACCGCCAACGTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGGCGCGCTTGCTCAACTTCgg < 1:410883/69‑1 (MQ=255) cGCCACCGCCAACGTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGGCGCGCTTGCTCAACTTCgg < 1:31199/69‑1 (MQ=255) cGCCACCGCCAACGTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGGCGCGCTTGCTCAACTTCgg < 1:52463/69‑1 (MQ=255) cGCCACCGCCAACGTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGGCGCGCTTGCTCAACTTCgg < 1:1353645/69‑1 (MQ=255) aCCGCCAACGTACAGCATCGTTTTTTGCGCTTTTGCCAGCATCTGACGCGCTTGCTc > 1:1725651/1‑57 (MQ=255) aCCGCCAACGTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGACGCGCTTGCTc > 1:978844/1‑57 (MQ=255) aCCGCCAACGTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGACGCGCTTGCTc > 1:1891617/1‑57 (MQ=255) aCCGCCAACGTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGACGCGCTTGCTc > 1:2582856/1‑57 (MQ=255) aCCGCCAACGTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGACGCGCTTGCTc > 1:2538243/1‑57 (MQ=255) aCCGCCAACGTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGACGCGCTTGCTc > 1:314970/1‑57 (MQ=255) aCCGCCAACGTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGACGCGCTTGCTc > 1:2404970/1‑57 (MQ=255) aCCGCCAACGTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGACGCGCTTGCTc > 1:3207282/1‑57 (MQ=255) aCCGCCAACGTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGACGCGCTTGCTc > 1:331674/1‑57 (MQ=255) aCCGCCAACGTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGACGCGCTTGCTc > 1:68770/1‑57 (MQ=255) aCCGCCAACGTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGACGCGCTTGCTc > 1:1634423/1‑57 (MQ=255) aCCGCCAACGTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGACGCGCTTGCTc > 1:1658251/1‑57 (MQ=255) aCCGCCAACGTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGACGCGCTTGCTc > 1:2087573/1‑57 (MQ=255) gTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGACGCGCTTGCTCGACTTCGGCATGTGAGAAAGt < 1:73947/69‑1 (MQ=255) gTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGACGCGCTTGCTCGACTTCGGCATGTGAGAAAGt < 1:721634/69‑1 (MQ=255) gTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGACGCGCTTGCTCGACTTCGGCATGTGAGAAAGt < 1:2470746/69‑1 (MQ=255) gTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGACGCGCTTGCTCGACTTCGGCATGTGAGAAAGt < 1:1009982/69‑1 (MQ=255) gTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGACGCGCTTGCTCGACTTCGGCATGTGAGAAAGt < 1:1179786/69‑1 (MQ=255) gTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGACGCGCTTGCTCGACTTCGGCATGTGAGAAAGt < 1:263371/69‑1 (MQ=255) gTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGACGCGCTTGCTCGACTTCGGCATGTGAGAAAGt < 1:2496031/69‑1 (MQ=255) gTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGACGCGCTTGCTCGACTTCGGCATGTGAGAAAGt < 1:1959682/69‑1 (MQ=255) gTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGACGCGCTTGCTCGACTTCGGCATGTGAGAAAGt < 1:2305186/69‑1 (MQ=255) cAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGGCGCGCTTGCTCAACTTCGGCATGTGGGAAAGTCa > 1:1590409/1‑68 (MQ=255) cAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGGCGCGCTTGCTCAACTTCGGCATGTGGGAAAGTCAc > 1:41481/1‑69 (MQ=255) cAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGGCGCGCTTGCTCAACTTCGGCATGTGGGAAAGTCAc > 1:3174302/1‑69 (MQ=255) cAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGGCGCGCTTGCTCAACTTCGGCATGTGGGAAAGTCAc > 1:1005111/1‑69 (MQ=255) ggTTTTTGCGCTTTTGCCAGCATCTGGCGCGCTTGCTCAACTTCGGCAtg > 1:1379150/1‑50 (MQ=255) ggTTTTTGCGCTTTTGCCAGCATCTGGCGCGCTTGCTCAACTTCGGCAtg > 1:505588/1‑50 (MQ=255) tttGCGCTTTTGCCAGCATCTGGCGCGCTTGCTCAACTTCGGCATGTGGGAAAGTCACTTCGTTTTCaa > 1:1046948/1‑69 (MQ=255) tttGCGCTTTTGCCAGCATCTGGCGCGCTTGCTCAACTTCGGCATGTGGGAAAGTCACTTCGTTTTCaa > 1:859587/1‑69 (MQ=255) tttGCGCTTTTGCCAGCATCTGACGCGCTTGCTCGACTTCGGCATGTGAGAAAGTCACTTCGTTTTCaa < 1:2323288/69‑1 (MQ=255) tttGCGCTTTTGCCAGCATCTGACGCGCTTGCTCGACTTCGGCATGTGAGAAAGTCACTTCGTTTTCaa < 1:804708/69‑1 (MQ=255) tttGCGCTTTTGCCAGCATCTGACGCGCTTGCTCGACTTCGGCATGTGAGAAAGTCACTTCGTTTTCaa < 1:74489/69‑1 (MQ=255) tttGCGCTTTTGCCAGCATCTGACGCGCTTGCTCGACTTCGGCATGTGAGAAAGTCACTTCGTTTTCaa < 1:1513518/69‑1 (MQ=255) tttGCGCTTTTGCCAGCATCTGACGCGCTTGCTCGACTTCGGCATGTGAGAAAGTCACTTCGTTTTCaa < 1:582538/69‑1 (MQ=255) tttGCGCTTTTGCCAGCATCTGACGCGCTTGCTCGACTTCGGCATGTGAGAAAGTCACTTCGTTTTCaa < 1:1867540/69‑1 (MQ=255) tttGCGCTTTTGCCAGCATCTGACGCGCTTGCTCGACTTCGGCATGTGAGAAAGTCACTTCGTTTTCaa < 1:3012504/69‑1 (MQ=255) tttGCGCTTTTGCCAGCATCTGACGCGCTTGCTCGACTTCGGCATGTGAGAAAGTCACTTCGTTTTCaa < 1:1940074/69‑1 (MQ=255) tttGCGCTTTTGCCAGCATCTGACGCGCTTGCTCGACTTCGGCATGTGAGAAAGTCACTTCGTTTTCaa < 1:232/69‑1 (MQ=255) tttGCGCTTTTGCCAGCATCTGACGCGCTTGCTCGACTTCGGCATGTGAGAAAGTCACTTCGTTTTCaa < 1:3295315/69‑1 (MQ=255) gcTTTTGCCAGCATCTGGCGCGCTTGCTCAACTTCGGCATGTGGGAAAGTCACTTCGTTTTCAACggt < 1:1759134/68‑1 (MQ=255) gcTTTTGCCAGCATCTGGCGCGCTTGCTCAACTTCGGCATGTGGGAAAGTCACTTCGTTTTCAACggt < 1:2563811/68‑1 (MQ=255) gcTTTTGCCAGCATCTGGCGCGCTTGCTCAACTTCGGCATGTGGGAAAGTCACTTCGTTTTCAACggt < 1:1451266/68‑1 (MQ=255) gcTTTTGCCAGCATCTGGCGCGCTTGCTCAACTTCGGCATGTGGGAAAGTCACTTCGTTTTCAACggt < 1:1443027/68‑1 (MQ=255) gcTTTTGCCAGCATCTGGCGCGCTTGCTCAACTTCGGCATGTGGGAAAGTCACTTCGTTTTCAACggt < 1:2015076/68‑1 (MQ=255) gcTTTTGCCAGCATCTGGCGCGCTTGCTCAACTTCGGCATGTGGGAAAGTCACTTCGTTTTCAACggt < 1:2258368/68‑1 (MQ=255) gcTTTTGCCAGCATCTGGCGCGCTTGCTCAACTTCGGCATGTGGGAAAGTCACTTCGTTTTCAACggt < 1:1103331/68‑1 (MQ=255) gcTTTTGCCAGCATCTGGCGCGCTTGCTCAACTTCGGCATGTGGGAAAGTCACTTCGTTTTCAACggt < 1:3019533/68‑1 (MQ=255) ccAGCATCTGGCGCGCTTGCTCAACTTCGGCATGTGGGAAAGTCACTTCGTTTTCAACGGTGGTGAAc < 1:2846924/68‑1 (MQ=255) ccAGCATCTGGCGCGCTTGCTCAACTTCGGCATGTGGGAAAGTCACTTCGTTTTCAACGGTGGTGAAc < 1:943435/68‑1 (MQ=255) ccAGCATCTGGCGCGCTTGCTCAACTTCGGCATGTGGGAAAGTCACTTCGTTTTCAACGGTGGTGAAc < 1:2351657/68‑1 (MQ=255) | TGCGCCATACCCACGCCACCGCCAACGTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGGCGCGCTTGCTCAACTTCGGCATGTGGGAAAGTCACTTCGTTTTCAACGGTGGTGAAC > minE/2447424‑2447543 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |