| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,447,498 | A→G | 57.6% | pseudogene (558/1647 nt) | ilvG ← | Mutation in protein compared to W3110: p.Glu327_Ilefs*223 Restoration of IlvG found in MG1655 (548 amino acids), fusion of N‑ter and C‑ter fragments ECK3760:JW3740+JW3741:b4488; large subunit of acetolactate synthase II |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,447,498 | 0 | A | G | 57.6% | 47.1 / 95.4 | 128 | pseudogene (558/1647 nt) | ilvG | Mutation in protein compared to W3110: p.Glu327_Ilefs*223 Restoration of IlvG found in MG1655 (548 amino acids), fusion of N‑ter and C‑ter fragments ECK3760:JW3740+JW3741:b4488; large subunit of acetolactate synthase II |
| Reads supporting (aligned to +/- strand): ref base A (23/31); new base G (16/57); total (39/89) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.90e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 128 total aligned reads displayed.
CCCACGCCACCGCCAACGTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGGCGCGCTTGCTCAACTTCGGCATGTGGGAAAGTCACTTCGTTTTCAACGGTGGTGAAC > minE/2447433‑2447543 | cccACGCCACCGCCAACGTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGGCGCGCTTGCTCAAc > 1:3306578/1‑68 (MQ=255)cccACGCCACCGCCAACGTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGGCGCGCTTGCTCAACt > 1:257941/1‑69 (MQ=255)cccACGCCACCGCCAACGTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGGCGCGCTTGCTCAACt > 1:2809467/1‑69 (MQ=255)cccACGCCACCGCCAACGTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGGCGCGCTTGCTCAACt > 1:1739046/1‑69 (MQ=255)cccACGCCACCGCCAACGTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGGCGCGCTTGCTCAACt > 1:2456711/1‑69 (MQ=255)cccACGCCACCGCCAACGTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGGCGCGCTTGCTCAACt > 1:3146068/1‑69 (MQ=255)cccACGCCACCGCCAACGTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGGCGCGCTTGCTCAACt > 1:2274010/1‑69 (MQ=255)cccACGCCACCGCCAACGTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGGCGCGCTTGCTCAACt > 1:1890697/1‑69 (MQ=255)cccACGCCACCGCCAACGTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGGCGCGCTTGCTAAACt > 1:1294227/1‑69 (MQ=255)cccACGCCACCGCCAACGTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGACGCGCTTGCTCGAc > 1:3144788/1‑68 (MQ=255)cccACGCCACCGCCAACGTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGACGCGCTTGCTCGACt < 1:1621807/69‑1 (MQ=255)cccACGCCACCGCCAACGTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGACGCGCTTGCTCGACt < 1:1801145/69‑1 (MQ=255)cccACGCCACCGCCAACGTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGACGCGCTTGCTCGACt > 1:1648969/1‑69 (MQ=255)cccACGCCACCGCCAACGTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGACGCGCTTGCTCGACt > 1:1825465/1‑69 (MQ=255)cccACGCCACCGCCAACGTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGACGCGCTTGCTCGACt > 1:1873453/1‑69 (MQ=255)cccACGCCACCGCCAACGTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGACGCGCTTGCTCGACt < 1:1896812/69‑1 (MQ=255)cccACGCCACCGCCAACGTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGACGCGCTTGCTCGACt > 1:2037292/1‑69 (MQ=255)cccACGCCACCGCCAACGTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGACGCGCTTGCTCGACt < 1:2070871/69‑1 (MQ=255)cccACGCCACCGCCAACGTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGACGCGCTTGCTCGACt < 1:3240149/69‑1 (MQ=255)cccACGCCACCGCCAACGTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGACGCGCTTGCTCGACt > 1:2241332/1‑69 (MQ=255)cccACGCCACCGCCAACGTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGACGCGCTTGCTCGACt > 1:2242942/1‑69 (MQ=255)cccACGCCACCGCCAACGTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGACGCGCTTGCTCGACt < 1:2246697/69‑1 (MQ=255)cccACGCCACCGCCAACGTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGACGCGCTTGCTCGACt < 1:2958353/69‑1 (MQ=255)cccACGCCACCGCCAACGTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGACGCGCTTGCTCGACt < 1:278775/69‑1 (MQ=255)cccACGCCACCGCCAACGTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGACGCGCTTGCTCGACt < 1:257765/69‑1 (MQ=255)cccACGCCACCGCCAACGTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGACGCGCTTGCTCGACt > 1:1296279/1‑69 (MQ=255)cccACGCCACCGCCAACGTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGACGCGCTTGCTCGACt < 1:983304/69‑1 (MQ=255)cccACGCCACCGCCAACGTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGACGCGCTTGCTCGACt > 1:1023588/1‑69 (MQ=255)cccACGCCACCGCCAACGTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGACGCGCTTGCTCGACt < 1:96522/69‑1 (MQ=255)cccACGCCACCGCCAACGTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGACGCGCTTGCTCGACt < 1:840469/69‑1 (MQ=255)cccACGCCACCGCCAACGTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGACGCGCTTGCTCGACt < 1:1113485/69‑1 (MQ=255)cccACGCCACCGCCAACGTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGACGCGCTTGCTCGACt < 1:1117103/69‑1 (MQ=255)cccACGCCACCGCCAACGTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGACGCGCTTGCTCGACt < 1:783611/69‑1 (MQ=255)cccACGCCACCGCCAACGTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGACGCGCTTGCTCGACt < 1:1223350/69‑1 (MQ=255)cccACGCCACCGCCAACGTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGACGCGCTTGCTCGACt < 1:1289143/69‑1 (MQ=255)cccACGCCACCGCCAACGTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGACGCGCTTGCTCGACt > 1:40904/1‑69 (MQ=255)cccACGCCACCGCCAACGTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGACGCGCTTGCTCGACt < 1:1424474/69‑1 (MQ=255)cccACGCCACCGCCAACGTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGACGCGCTTGCTCGACt > 1:581177/1‑69 (MQ=255) ccACGCCACCGCCAACGTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGACGCGCTTGCTCGACt < 1:2415925/68‑1 (MQ=255) cGCCACCGCCAACTTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGGCGCGCTTGCTCAACTTCgg < 1:1987032/69‑1 (MQ=255) cGCCACCGCCAACGTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGGCGCGCTTGCTCAACTTCgg < 1:3307924/69‑1 (MQ=255) cGCCACCGCCAACGTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGGCGCGCTTGCTCAACTTCgg < 1:1514149/69‑1 (MQ=255) cGCCACCGCCAACGTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGGCGCGCTTGCTCAACTTCgg < 1:410883/69‑1 (MQ=255) cGCCACCGCCAACGTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGGCGCGCTTGCTCAACTTCgg < 1:1799879/69‑1 (MQ=255) cGCCACCGCCAACGTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGGCGCGCTTGCTCAACTTCgg < 1:3068942/69‑1 (MQ=255) cGCCACCGCCAACGTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGGCGCGCTTGCTCAACTTCgg < 1:31199/69‑1 (MQ=255) cGCCACCGCCAACGTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGGCGCGCTTGCTCAACTTCgg < 1:1844807/69‑1 (MQ=255) cGCCACCGCCAACGTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGGCGCGCTTGCTCAACTTCgg < 1:2318567/69‑1 (MQ=255) cGCCACCGCCAACGTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGGCGCGCTTGCTCAACTTCgg < 1:1198574/69‑1 (MQ=255) cGCCACCGCCAACGTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGGCGCGCTTGCTCAACTTCgg < 1:3413508/69‑1 (MQ=255) cGCCACCGCCAACGTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGGCGCGCTTGCTCAACTTCgg < 1:52463/69‑1 (MQ=255) cGCCACCGCCAACGTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGGCGCGCTTGCTCAACTTCgg < 1:2125384/69‑1 (MQ=255) cGCCACCGCCAACGTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGGCGCGCTTGCTCAACTTCgg < 1:1353645/69‑1 (MQ=255) gTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGACGCGCTTGCTCGACTTCGGCATGTGAGAAAGt < 1:73947/69‑1 (MQ=255) gTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGACGCGCTTGCTCGACTTCGGCATGTGAGAAAGt < 1:729111/69‑1 (MQ=255) gTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGACGCGCTTGCTCGACTTCGGCATGTGAGAAAGt < 1:412732/69‑1 (MQ=255) gTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGACGCGCTTGCTCGACTTCGGCATGTGAGAAAGt < 1:1009982/69‑1 (MQ=255) gTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGACGCGCTTGCTCGACTTCGGCATGTGAGAAAGt < 1:366224/69‑1 (MQ=255) gTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGACGCGCTTGCTCGACTTCGGCATGTGAGAAAGt < 1:721634/69‑1 (MQ=255) gTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGACGCGCTTGCTCGACTTCGGCATGTGAGAAAGt < 1:631905/69‑1 (MQ=255) gTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGACGCGCTTGCTCGACTTCGGCATGTGAGAAAGt < 1:263371/69‑1 (MQ=255) gTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGACGCGCTTGCTCGACTTCGGCATGTGAGAAAGt < 1:2496031/69‑1 (MQ=255) gTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGACGCGCTTGCTCGACTTCGGCATGTGAGAAAGt < 1:1179786/69‑1 (MQ=255) gTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGACGCGCTTGCTCGACTTCGGCATGTGAGAAAGt < 1:1479242/69‑1 (MQ=255) gTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGACGCGCTTGCTCGACTTCGGCATGTGAGAAAGt < 1:2470746/69‑1 (MQ=255) gTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGACGCGCTTGCTCGACTTCGGCATGTGAGAAAGt < 1:1959682/69‑1 (MQ=255) gTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGACGCGCTTGCTCGACTTCGGCATGTGAGAAAGt < 1:2305186/69‑1 (MQ=255) cAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGGCGCGCTTGCTCAACTTCGGCATGTGGGAAAGTCa > 1:1590409/1‑68 (MQ=255) cAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGGCGCGCTTGCTCAACTTCGGCATGTGGGAAAGTCAc > 1:1005111/1‑69 (MQ=255) cAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGGCGCGCTTGCTCAACTTCGGCATGTGGGAAAGTCAc > 1:3174302/1‑69 (MQ=255) cAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGGCGCGCTTGCTCAACTTCGGCATGTGGGAAAGTCAc > 1:2355151/1‑69 (MQ=255) ggTTTTTGCGCTTTTGCCAGCATCTGGCGCGCTTGCTCAACTTCGGCAtg > 1:505588/1‑50 (MQ=255) ggTTTTTGCGCTTTTGCCAGCATCTGGCGCGCTTGCTCAACTTCGGCAtg > 1:1379150/1‑50 (MQ=255) tttGCGCTTTTGCCAGCATCTGGCGCGCTTGCTCAACTTCGGCATGTGGGAAAGTCACTTCGTTTTCaa > 1:1046948/1‑69 (MQ=255) tttGCGCTTTTGCCAGCATCTGGCGCGCTTGCTCAACTTCGGCATGTGGGAAAGTCACTTCGTTTTCaa > 1:859587/1‑69 (MQ=255) tttGCGCTTTTGCCAGCATCTGACGCGCTTGCTCGACTTCGGCATGTGAGAAAGTCACTTCGTTTTCaa < 1:1151428/69‑1 (MQ=255) tttGCGCTTTTGCCAGCATCTGACGCGCTTGCTCGACTTCGGCATGTGAGAAAGTCACTTCGTTTTCaa < 1:582538/69‑1 (MQ=255) tttGCGCTTTTGCCAGCATCTGACGCGCTTGCTCGACTTCGGCATGTGAGAAAGTCACTTCGTTTTCaa < 1:804708/69‑1 (MQ=255) tttGCGCTTTTGCCAGCATCTGACGCGCTTGCTCGACTTCGGCATGTGAGAAAGTCACTTCGTTTTCaa < 1:2323288/69‑1 (MQ=255) tttGCGCTTTTGCCAGCATCTGACGCGCTTGCTCGACTTCGGCATGTGAGAAAGTCACTTCGTTTTCaa < 1:514234/69‑1 (MQ=255) tttGCGCTTTTGCCAGCATCTGACGCGCTTGCTCGACTTCGGCATGTGAGAAAGTCACTTCGTTTTCaa < 1:1513518/69‑1 (MQ=255) tttGCGCTTTTGCCAGCATCTGACGCGCTTGCTCGACTTCGGCATGTGAGAAAGTCACTTCGTTTTCaa < 1:3012504/69‑1 (MQ=255) tttGCGCTTTTGCCAGCATCTGACGCGCTTGCTCGACTTCGGCATGTGAGAAAGTCACTTCGTTTTCaa < 1:1867540/69‑1 (MQ=255) tttGCGCTTTTGCCAGCATCTGACGCGCTTGCTCGACTTCGGCATGTGAGAAAGTCACTTCGTTTTCaa < 1:1940074/69‑1 (MQ=255) tttGCGCTTTTGCCAGCATCTGACGCGCTTGCTCGACTTCGGCATGTGAGAAAGTCACTTCGTTTTCaa < 1:960730/69‑1 (MQ=255) tttGCGCTTTTGCCAGCATCTGACGCGCTTGCTCGACTTCGGCATGTGAGAAAGTCACTTCGTTTTCaa < 1:3295315/69‑1 (MQ=255) tttGCGCTTTTGCCAGCATCTGACGCGCTTGCTCGACTTCGGCATGTGAGAAAGTCACTTCGTTTTCaa < 1:2083853/69‑1 (MQ=255) tttGCGCTTTTGCCAGCATCTGACGCGCTTGCTCGACTTCGGCATGTGAGAAAGTCACTTCGTTTTCaa < 1:137758/69‑1 (MQ=255) gcTTTTGCCAGCATCTGGCGCGCTTGCTCTACTTCGGCATGTGGGAAAGTCACTTCGTTTTCAACggt < 1:1197628/68‑1 (MQ=255) gcTTTTGCCAGCATCTGGCGCGCTTGCTCAACTTCGGCATGTGGGAAAGTCACTTCGTTTTCAACggt < 1:1103331/68‑1 (MQ=255) gcTTTTGCCAGCATCTGGCGCGCTTGCTCAACTTCGGCATGTGGGAAAGTCACTTCGTTTTCAACggt < 1:2563811/68‑1 (MQ=255) gcTTTTGCCAGCATCTGGCGCGCTTGCTCAACTTCGGCATGTGGGAAAGTCACTTCGTTTTCAACggt < 1:1451266/68‑1 (MQ=255) gcTTTTGCCAGCATCTGGCGCGCTTGCTCAACTTCGGCATGTGGGAAAGTCACTTCGTTTTCAACggt < 1:6118/68‑1 (MQ=255) gcTTTTGCCAGCATCTGGCGCGCTTGCTCAACTTCGGCATGTGGGAAAGTCACTTCGTTTTCAACggt < 1:1443027/68‑1 (MQ=255) gcTTTTGCCAGCATCTGGCGCGCTTGCTCAACTTCGGCATGTGGGAAAGTCACTTCGTTTTCAACggt < 1:1759134/68‑1 (MQ=255) gcTTTTGCCAGCATCTGGCGCGCTTGCTCAACTTCGGCATGTGGGAAAGTCACTTCGTTTTCAACggt < 1:3019533/68‑1 (MQ=255) gcTTTTGCCAGCATCTGGCGCGCTTGCTCAACTTCGGCATGTGGGAAAGTCACTTCGTTTTCAACggt < 1:319570/68‑1 (MQ=255) ccAGCATCTGGCGCGCTTGCTCAACTTCGGCATGTGGGAAAGTCACTTCGTTTTCAACGGTGGTGAAc < 1:2846924/68‑1 (MQ=255) ccAGCATCTGGCGCGCTTGCTCAACTTCGGCATGTGGGAAAGTCACTTCGTTTTCAACGGTGGTGAAc < 1:2909139/68‑1 (MQ=255) ccAGCATCTGGCGCGCTTGCTCAACTTCGGCATGTGGGAAAGTCACTTCGTTTTCAACGGTGGTGAAc < 1:2351657/68‑1 (MQ=255) | CCCACGCCACCGCCAACGTACAGCATCGGTTTTTGCGCTTTTGCCAGCATCTGGCGCGCTTGCTCAACTTCGGCATGTGGGAAAGTCACTTCGTTTTCAACGGTGGTGAAC > minE/2447433‑2447543 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |