| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,449,128 | A→G | 51.2% | Q171Q (CAA→CAG) | yifB → | predicted bifunctional protein, enzyme and transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,449,128 | 0 | A | G | 51.2% | ‑1.1 / 53.0 | 62 | Q171Q (CAA→CAG) | yifB | predicted bifunctional protein, enzyme and transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base A (17/13); new base G (14/17); total (31/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.46e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01 | |||||||||||
AAAGATAACGAAGATGAAGTGGGGCTAATTAACGGTGAAGGATGCCTGATAGCCGATCATCTGCAAGCTGTCTGTGCGTTTCTGGAAGGTAAGCACGCTCTCGAACGCCCGAAACCAACTGATGCAGTATCC > minE/2449063‑2449194 | aaaGATAACGAAGATGAAGTGGGGCTAATTAACGGTGAAGGTTGCCTGATAGCCGATCATCTGCAAGct < 1:739405/69‑1 (MQ=255)aaaGATAACGAAGATGAAGTGGGGCTAATTAACGGTGAAGGATGCCTGATAGCCGATCATCTGCAAGct < 1:2589161/69‑1 (MQ=255)aaaGATAACGAAGATGAAGTGGGGCTAATTAACGGTGAAGGATGCCTGATAGCCGATCATCTGCAAGct < 1:353560/69‑1 (MQ=255)aaaGATAACGAAGATGAAGTGGGGCTAATTAACGGTGAAGGATGCCTGATAGCCGATCATCTGCAAGct < 1:13098/69‑1 (MQ=255) aGATAACGAAGATGAAGTGGGGCTAATTAACGGTGAAGGATGCCTGATAGCCGATCATCTGCAAGct < 1:1043847/67‑1 (MQ=255) tgggCTAATTAACGGTGAAGGATGCCTGATAGCCGATCATCTGCAGGCTGTCTGCGCGTTTCTGGAAgg < 1:1041759/68‑1 (MQ=255) ggggCTAATTAACGGTGAAGGATGCCTGATAGCCGATCATCTGCAGGCTGTCTGCGCGTTTCTGGAAgg < 1:2097779/69‑1 (MQ=255) ggggCTAATTAACGGTGAAGGATGCCTGATAGCCGATCATCTGCAGGCTGTCTGCGCGTTTCTGGAAgg < 1:2372088/69‑1 (MQ=255) ggggCTAATTAACGGTGAAGGATGCCTGATAGCCGATCATCTGCAGGCTGTCTGCGCGTTTCTGGAAgg < 1:2585365/69‑1 (MQ=255) ggggCTAATTAACGGTGAAGGATGCCTGATAGCCGATCATCTGCAGGCTGTCTGCGCGTTTCTGGAAgg < 1:1856632/69‑1 (MQ=255) ggggCTAATTAACGGTGAAGGATGCCTGATAGCCGATCATCTGCAGGCTGTCTGCGCGTTTCTGGAAgg < 1:2725655/69‑1 (MQ=255) ggggCTAATTAACGGTGAAGGATGCCTGATAGCCGATCATCTGCAGGCTGTCTGCGCGTTTCTGGAAgg < 1:1665840/69‑1 (MQ=255) ggggCTAATTAACGGTGAAGGATGCCTGATAGCCGATCATCTGCAGGCTGTCTGCGCGTTTCTGGAAgg < 1:3306960/69‑1 (MQ=255) ggggCTAATTAACGGTGAAGGATGCCTGATAGCCGATCATCTGCAGGCTGTCTGCGCGTTTCTGGAAgg < 1:1296537/69‑1 (MQ=255) ggggCTAATTAACGGTGAAGGATGCCTGATAGCCGATCATCTGCAGGCTGTCTGCGCGTTTCTGGAAgg < 1:551541/69‑1 (MQ=255) ggggCTAATTAACGGTGAAGAATGCCTGATAGCCGATCATCTGCAGGCTGTCTGCGCGTTTCTGGAAgg < 1:1599631/69‑1 (MQ=255) ggggCTAATAAACGGTGAAGGATGCCTGATAGCCGATCATCTGCAGGCTGTCTGCGCGTTTCTGGAAgg < 1:761008/69‑1 (MQ=255) aTTAACGGTGAAGGATGCCTGATAGCCGATCATCTGCAAGCTGTCTGTGCGTTTCTGGAAGGTAAGCAc > 1:1356680/1‑69 (MQ=255) aTTAACGGTGAAGGATGCCTGATAGCCGATCATCTGCAAGCTGTCTGTGCGTTTCTGGAAGGTAAGCAc > 1:282037/1‑69 (MQ=255) aTTAACGGTGAAGGATGCCTGATAGCCGATCATCTGCAAGCTGTCTGTGCGTTTCTGGAAGGTAAGCAc > 1:134519/1‑69 (MQ=255) aTTAACGGTGAAGGATGCCTGATAGCCGATCATCTGCAAGCTGTCTGTGCGTTTCTGGAAGGTAAGCAc > 1:185002/1‑69 (MQ=255) aTTAACGGTGAAGGATGCCTGATAGCCGATCATCTGCAAGCTGTCTGTGCGTTTCTGGAAGGTAAGCAc > 1:533688/1‑69 (MQ=255) aTTAACGGTGAAGGATGCCTGATAGCCGATCATCTGCAAGCTGTCTGTGCGTTTCTGGAAGGTAAGCAc > 1:1887038/1‑69 (MQ=255) tAACGGTGAAGGATGCCTGATAGCCGATCATCTGCAAGCTGTCTGTGCGTTTCTGGAAGGTAAGCACg < 1:935896/68‑1 (MQ=255) gTGAAGGATGCCTGATAGCCGATCATCTGCAAGCTGTCTGTGCGTTTCTGGAAGGTAAGCACGCTCTCg < 1:1389444/69‑1 (MQ=255) tGCCTGATAGCCGATCATCTGCAAGCTGTCTGTGCGTTTCTGGAAGGTAAGCACGCTCTCGAACGccc > 1:2997321/1‑68 (MQ=255) cTGATAGCCGATCATCTGCAGGCTGTCTGCGCGTTTCTGGAAGGTAAGCACGCTCTCGAACAccc > 1:1152753/1‑65 (MQ=255) cTGATAGCCGATCATCTGCAGGCTGTCTGCGCGTTTCTGGAAGGTAAGCACGCTCTCGAACACCCGaa > 1:201694/1‑68 (MQ=255) cTGATAGCCGATCATCTGCAGGCTGTCTGCGCGTTTCTGGAAGGTAAGCACGCTCTCGAACACCCGaa > 1:914199/1‑68 (MQ=255) cTGATAGCCGATCATCTGCAGGCTGTCTGCGCGTTTCTGGAAGGTAAGCACGCTCTCGAACACCCGaa > 1:1231485/1‑68 (MQ=255) cTGATAGCCGATCATCTGCAGGCTGTCTGCGCGTTTCTGGAAGGTAAGCACGCTCTCGAACACCCGaa > 1:1279500/1‑68 (MQ=255) cTGATAGCCGATCATCTGCAGGCTGTCTGCGCGTTTCTGGAAGGTAAGCACGCTCTCGAACACCCGaa > 1:1337024/1‑68 (MQ=255) cTGATAGCCGATCATCTGCAGGCTGTCTGCGCGTTTCTGGAAGGTAAGCACGCTCTCGAACACCCGaa > 1:3219560/1‑68 (MQ=255) cTGATAGCCGATCATCTGCAGGCTGTCTGCGCGTTTCTGGAAGGTAAGCACGCTCTCGAACACCCGaa > 1:3096494/1‑68 (MQ=255) cTGATAGCCGATCATCTGCAGGCTGTCTGCGCGTTTCTGGAAGGTAAGCACGCTCTCGAACACCCGaa > 1:3027641/1‑68 (MQ=255) cTGATAGCCGATCATCTGCAGGCTGTCTGCGCGTTTCTGGAAGGTAAGCACGCTCTCGAACACCCGaa > 1:2908961/1‑68 (MQ=255) cTGATAGCCGATCATCTGCAGGCTGTCTGCGCGTTTCTGGAAGGTAAGCACGCTCTCGAACACCCGaa > 1:2698910/1‑68 (MQ=255) cTGATAGCCGATCATCTGCAGGCTGTCTGCGCGTTTCTGGAAGGTAAGCACGCTCTCGAACACCCGaa > 1:2035343/1‑68 (MQ=255) cTGATAGCCGATCATCTGCAGGCTGTCTGCGCGTTTCTGGAAGGTAAGCACGCTCTCGAACACCCGaa > 1:2187993/1‑68 (MQ=255) cTGATAACCGATCATCTGCAGGCTGTCTGCGCGTTTCTGGAAGGTAAGCACGCTCTCGAACACCCGaa > 1:1991/1‑68 (MQ=255) gATAGCCGATCATCTGCATGCTGTCTGTGCGTTTCTGGAAGGTAAGCACGCTCTCGAACGCCCGAAAcc < 1:2002059/69‑1 (MQ=255) gATAGCCGATCATCTGCAAGCTGTCTGTGCGTTTCTGGAAGGTAAGCACGCTCTCGAACGCCCGAAAcc < 1:2816202/69‑1 (MQ=255) gATAGCCGATCATCTGCAAGCTGTCTGTGCGTTTCTGGAAGGTAAGCACGCTCTCGAACGCCCGAAAcc < 1:263278/69‑1 (MQ=255) gATAGCCGATCATCTGCAAGCTGTCTGTGCGTTTCTGGAAGGTAAGCACGCTCTCGAACGCCCGAAAcc < 1:481784/69‑1 (MQ=255) gATAGCCGATCATCTGCAAGCTGTCTGTGCGTTTCTGGAAGGTAAGCACGCTCTCGAACGCCCGAAAcc < 1:3062869/69‑1 (MQ=255) gATAGCCGATCATCTGCAAGCTGTCTGTGCGTTTCTGGAAGGTAAGCACGCTCTCGAACGCCCGAAAcc < 1:3292829/69‑1 (MQ=255) gCCGATCATCTGCAAGCTGTCTGTGCGTTTCTGGAAGGTAAGCACGCTCTCGAACGCCCGAAACCAACt > 1:2115206/1‑69 (MQ=255) gCCGATCATCTGCAAGCTGTCTGTGCGTTTCTGGAAGGTAAGCACGCTCTCGAACGCCCGAAACCAACt > 1:3370659/1‑69 (MQ=255) cGATCATCTGCAAGCTGTCTGTGCGTTTCTGGAAGGTAAGCACGctct > 1:1021962/1‑48 (MQ=255) cGATCATCTGCAAGCTGTCTGTGCGTTTCTGGAAGGTAAGCACGCTCTCGAACGCCCGAAACCAACTg > 1:17899/1‑68 (MQ=255) cGATCATCTGCAAGCTGTCTGTGCGTTTCTGGAAGGTAAGCACGCTCTCGAACGCCCGAAACCAACTGa > 1:2251165/1‑69 (MQ=255) cGATCATCTGCAAGCTGTCTGTGCGTTTCTGGAAGGTAAGCACGCTCTCGAACGCCCGAAACCAACTGa > 1:2586502/1‑69 (MQ=255) cGATCATCTGCAAGCTGTCTGTGCGTTTCTGGAAGGTAAGCACGCTCTCGAACGCCCGAAACCAACTGa > 1:674143/1‑69 (MQ=255) cGATCATCTGCAAGCTGTCTGTGCGTTTCTGGAAGGTAAGCACGCTCTCGAACGCCCGAAACCAACTGa > 1:2918755/1‑69 (MQ=255) cGATCATCTGCAAGCTGTCTGTGCGTTTCTGGAAGGTAAGCACGCTCTCGAACGCCCGAAACCAACTGa > 1:1980186/1‑69 (MQ=255) atcTGCAGGCTGTCTGCGCGTTTCTGGAAGGTAAGCACGCTCTCGAACACCCGAAACCAACTAATGCAg < 1:2537010/69‑1 (MQ=255) atcTGCAGGCTGTCTGCGCGTTTCTGGAAGGTAAGCACGCTCTCGAACACCCGAAACCAACTAATGCAg < 1:3057307/69‑1 (MQ=255) atcTGCAGGCTGTCTGCGCGTTTCTGGAAGGTAAGCACGCTCTCGAACACCCGAAACCAACTAATGCAg < 1:1299031/69‑1 (MQ=255) atcTGCAGGCTGTCTGCGCGTTTCTGGAAGGTAAGCACGCTCTCGAACACCCGAAACCAACTAATGCAg < 1:2928382/69‑1 (MQ=255) atcTGCAAGCTGTCTGTGCGTTTCTGGAAGGTAAGCACGCTATCGAACGCCCGAAACCAACTGATGCAg < 1:3109224/69‑1 (MQ=255) tcTGCAGGCTGTCTGCGCGTTTCTGGAAGGTAAGCACGCTCTCGAACACCCGAAACCAACTAATGCAg < 1:1299442/68‑1 (MQ=255) cAAGCTGTCTGTGCGTTTCTGGAAGGTAAGCACGCTCTCGAACGCCCGAAACCAACTGATGCAGTATcc > 1:2246788/1‑69 (MQ=255) | AAAGATAACGAAGATGAAGTGGGGCTAATTAACGGTGAAGGATGCCTGATAGCCGATCATCTGCAAGCTGTCTGTGCGTTTCTGGAAGGTAAGCACGCTCTCGAACGCCCGAAACCAACTGATGCAGTATCC > minE/2449063‑2449194 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |