| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 208,558 | G→A | 31.8% | E190K (GAG→AAG) | dkgB → | 2,5‑diketo‑D‑gluconate reductase B |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 208,558 | 0 | G | A | 31.8% | 106.4 / 57.5 | 116 | E190K (GAG→AAG) | dkgB | 2,5‑diketo‑D‑gluconate reductase B |
| Reads supporting (aligned to +/- strand): ref base G (44/35); new base A (16/21); total (60/56) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.36e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01 | |||||||||||
Only 100 of 116 total aligned reads displayed.
TAAACAGCACGGCATCCATATTACTTCCTATATGACGCTGGCGTATGGTAAGGCCCTGAAAGATGAGGTTATTGCTCGTATCGCAGCTAAACACAATGCGACTCCGGCACAAGTGATTCTGGCGTGGGCTAT > minE/208494‑208625 | tAAACAGCACGGCATCCATATTACTTCCTATATGACGCTGGCGTATGGTAAGGCCCTGAAAGATGAGGt < 1:1732815/69‑1 (MQ=255)tAAACAGCACGGCATCCATATTACTTCCTATATGACGCTGGCGTATGGTAAGGCCCTGAAAGATGAGGt < 1:3038428/69‑1 (MQ=255) cATATTACTTCCTATATGACGCTGGCGTATGGTAAGGCCCTGAAAGATGAGGTTAt > 1:2633057/1‑56 (MQ=255) cATATTACTTCCTATATGACGCTGGCGTATGGTAAGGCCCTGAAAGATGAGGTTAt > 1:865992/1‑56 (MQ=255) cATATTACTTCCTATATGACGCTGGCGTATGGTAAGGCCCTGAAAGATGAGGTTAt > 1:3419316/1‑56 (MQ=255) cATATTACTTCCTATATGACGCTGGCGTATGGTAAGGCCCTGAAAGATGAGGTTAt > 1:435871/1‑56 (MQ=255) cATATTACTTCCTATATGACGCTGGCGTATGGTAAGGCCCTGAAAGATGAGGTTAt > 1:2916100/1‑56 (MQ=255) cATATTACTTCCTATATGACGCTGGCGTATGGTAAGGCCCTGAAAGATGAGGTTAt > 1:1398986/1‑56 (MQ=255) cATATTACTTCCTATATGACGCTGGCGTATGGTAAGGCCCTGAAAGATGAGGTTAt > 1:1800249/1‑56 (MQ=255) cATATTACTTCCTATATGACGCTGGCGTATGGTAAGGCCCTGAAAGATGAGGTTATTGCTCGTATCGCa > 1:1003909/1‑69 (MQ=255) cATATTACTTCCTATATGACGCTGGCGTATGGTAAGGCCCTGAAAGATGAGGTTATTGCTCGTATCGCa > 1:1484985/1‑69 (MQ=255) cATATTACTTCCTATATGACGCTGGCGTATGGTAAGGCCCTGAAAGATGAGGTTATTGCTCGTATCGCa > 1:12262/1‑69 (MQ=255) cATATTACTTCCTATATGACGCTGGCGTATGGTAAGGCCATGAAAGATGAGGTTAt > 1:567644/1‑56 (MQ=255) ttACTTCCTATATGACGCTGGCGTATGGTAAGGCCCTGAAAGATGAGGTTATTGCTCg > 1:3277643/1‑58 (MQ=255) ttACTTCCTATATGACGCTGGCGTATGGTAAGGCCCTGAAAGATGAGGTTATTGCTCGTATCGCAGCt > 1:1326627/1‑68 (MQ=255) ttACTTCCTATATGACGCTGGCGTATGGTAAGGCCCTGAAAGATGAGGTTATTGCTCGTATCGCAGCTa > 1:2101360/1‑69 (MQ=255) ttACTTCCTATATGACGCTGGCGTATGGTAAGGCCCTGAAAGATGAGGTTATTGCTCGTATCGCAGCTa > 1:2060617/1‑69 (MQ=255) ttACTTCCTATATGACGCTGGCGTATGGTAAGGCCCTGAAAGATGAGGTTATTGCTCGTATCGCAGCTa > 1:705270/1‑69 (MQ=255) ttACTTCCTATATGACGCTGGCGTATGGTAAGGCCCTGAAAGATGAGGTTATTGCTCGTATCGCAGCTa > 1:2268360/1‑69 (MQ=255) ttACTTCCTATATGACGCTGGCGTATGGTAAGGCCCTGAAAGATGAGGTTATTGCTCGTATCGCAGCTa > 1:250276/1‑69 (MQ=255) ttACTTCCTATATGACGCTGGCGTATGGTAAGGCCCTGAAAGATGAGGTTATTGCTCGTATCGCAGCTa > 1:25895/1‑69 (MQ=255) ttACTTCCTATATGACGCTGGCGTATGGTAAGGCCCTGAAAGATGAGGTTATTGCTCGTATCGCAGCTa > 1:1948819/1‑69 (MQ=255) ttACTTCCTATATGACGCTGGCGTATGGTAAGGCCCTGAAAGATGAGGTTATTGCTCGTATCGCAGCTa > 1:1968145/1‑69 (MQ=255) ttACTTCCTATATGACGCTGGCGTATGGTAAGGCCCTGAAAGATGAGGTTATTGCTCGTATCGCAGCTa > 1:2676355/1‑69 (MQ=255) ttACTTCCTATATGACGCTGGCGTATGGTAAGGCCCTGAAAGATGAGGTTATTGCTCGTATCGCAGCTa > 1:58341/1‑69 (MQ=255) ttACTTCCTATATGACGCTGGCGTATGGTAAGGCCCTGAAAGATGAGGTTATTGCTCGTATCGCAGCTa > 1:1498611/1‑69 (MQ=255) ttACTTCCTATATGACGCTGGCGTATGGTAAGGCCCTGAAAGATGAGGTTATTGCTCGTATCGCAGCTa > 1:1389836/1‑69 (MQ=255) ttACTTCCTATATGACGCTGGCGTATGGTAAGGCCCTGAAAGATGAGGTTATTGCTCGTATCGCAGCTa > 1:3216787/1‑69 (MQ=255) ttACTTCCTATATGACGCTGGCGTATGGTAAGGCCCTGAAAGATGAGGTTATTGCTCGTATCGCAGCTa > 1:397562/1‑69 (MQ=255) ttACTTCCTAAATGACGCTGGCGTATGGTAAGGCCCTGAAAGATGAGGTTATTGCTCGTATCGCAGCTa > 1:691795/1‑69 (MQ=255) aCTTCCTATATGACGCTGGCGTATGGTAAGGCCCTGAAAGATGAGGTTATTGCTCGTATCGCAGCTaaa < 1:2822245/69‑1 (MQ=255) aCTTCCTATATGACGCTGGCGTATGGTAAGGCCCTGAAAGATGAGGTTATTGCTCGTATCGCAGCTaaa < 1:681717/69‑1 (MQ=255) aCTTCCTATATGACGCTGGCGTATGGTAAGGCCCTGAAAGATGAGGTTATTGCTCGTATCGCAGCTaaa < 1:3044561/69‑1 (MQ=255) aCTTCCTATATGACGCTGGCGTATGGTAAGGCCCTGAAAGATGAGGTTATTGCTCGTATCGCAGCTaaa < 1:3117586/69‑1 (MQ=255) aCTTCCTATATGACGCTGGCGTATGGTAAGGCCCTGAAAGATGAGGTTATTGCTCGTATCGCAGCTaaa < 1:2150722/69‑1 (MQ=255) aCTTCCTATATGACGCTGGCGTATGGTAAGGCCCTGAAAGATGAGGTTATTGCTCGTATCGCAGCTaaa < 1:1032590/69‑1 (MQ=255) aCTTCCTATATGACGCTGGCGTATGGTAAGGCCCTGAAAGATGAGGTTATTGCTCGTATCGCAGCTaaa < 1:1088427/69‑1 (MQ=255) cTTCCTATATGACGCTGGCGTATGGTAAGGCCCTGAAAGATGAGGTTATTGCTCGTATCGCAGCTaaa < 1:1488852/68‑1 (MQ=255) cTTCCTATATGACGCTGGCGTATGGTAAGGCCCTGAAAGATGAGGTTATTGCTCGTATCGCAGCTaaa < 1:627091/68‑1 (MQ=255) cTTCCTATATGACGCTGGCGTATGGTAAGGCCCTGAAAGATGAGGTTATTGCTCGTATCGCAGCTaaa < 1:2476926/68‑1 (MQ=255) cTTCCTATATGACGCTGGCGTATGGTAAGGCCCTGAAAGATGAGGTTATTGCTCGTATCGCAGCTaaa < 1:2968192/68‑1 (MQ=255) ttCCTATATGACTCTGGCGTATGGCAATGCACTGAAAGATAAGGTTATTGCTc > 1:1893364/1‑53 (MQ=255) ttCCTATATGACTCTGGCGTATGGCAATGCACTGAAAGATAAGGTTATTGCTCg > 1:3410963/1‑54 (MQ=255) ttCCTATATGACTCTGGCGTATGGCAATGCACTGAAAGATAAGGTTATTGCTCg > 1:2927005/1‑54 (MQ=255) ttCCTATATGACTCTGGCGTATGGCAATGCACTGAAAGATAAGGTTATTGCTCg > 1:183921/1‑54 (MQ=255) ttCCTATATGACTCTGGCGTATGGCAATGCACTGAAAGATAAGGTTATTGCTCg > 1:2898730/1‑54 (MQ=255) ttCCTATATGACTCTGGCGTATGGCAATGCACTGAAAGATAAGGTTATTGCTCg > 1:1493334/1‑54 (MQ=255) ttCCTATATGACTCTGGCGTATGGCAATGCACTGAAAGATAAGGTTATTGCTCg > 1:281381/1‑54 (MQ=255) ttCCTATATGACTCTGGCGTATGGCAATGCACTGAAAGATAAGGTTATTGCTCg > 1:1625706/1‑54 (MQ=255) ttCCTATATGACTCTGGCGTATGGCAATGCACTGAAAGATAAGGTTATTGCTCg > 1:1679642/1‑54 (MQ=255) ttCCTATATGACTCTGGCGTATGGCAATGCACTGAAAGATAAGGTTATTGCTCg > 1:2584853/1‑54 (MQ=255) ttCCTATATGACTCTGGCGTATGGCAATGCACTGAAAGATAAGGTTATTGCTCg > 1:432478/1‑54 (MQ=255) ttCCTATATGACTCTGGCGTATGGCAATGCACTGAAAGATAAGGTTATTGCTCg > 1:2061705/1‑54 (MQ=255) ttCCTATATGACTCTGGCGTATGGCAATGCACTGAAAGATAAGGTTATTGCTCg > 1:1683750/1‑54 (MQ=255) ttCCTATATGACTCTGGCGTATGGCAATGCACTGAAAGATAAGGTTATTGCTCg > 1:838319/1‑54 (MQ=255) ttCCTATATGACTCTGGCGTATGGCAATGCACTGAAAGATAAGGTTATTGCTCg > 1:648755/1‑54 (MQ=255) tCCTATATGACTCTGGCGTATGGCAATGCACTGAAAGATAAGGTTATTGCTCGTATTGCAGCTAAaca < 1:2940235/68‑1 (MQ=255) tCCTATATGACTCTGGCGTATGGCAATGCACTGAAAGATAAGGTTATTGCTCGTATTGCAGCTAAaca < 1:2023031/68‑1 (MQ=255) tCCTATATGACTCTGGCGTATGGCAATGCACTGAAAGATAAGGTTATTGCTCGTATTGCAGCTAAaca < 1:1814549/68‑1 (MQ=255) tCCTATATGACTCTGGCGTATGGCAATGCACTGAAAGATAAGGTTATTGCTCGTATTGCAGCTAAaca < 1:3368111/68‑1 (MQ=255) tCCTATATGACTCTGGCGTATGGCAATGCACTGAAAGATAAGGTTATTGCTCGTATTGCAGCTAAaca < 1:1787404/68‑1 (MQ=255) tCCTATATGACTCTGGCGTATGGCAATGCACTGAAAGATAAGGTTATTGCTCGTATTGCAGCTAAaca < 1:1088885/68‑1 (MQ=255) tCCTATATGACTCTGGCGTATGGCAATGCACTGAAAGATAAGGTTATTGCTCGTATTGCAGCTAAaca < 1:3055588/68‑1 (MQ=255) tCCTATATGACTCTGGCGTATGGCAATGCACTGAAAGATAAGGTTATTGCTCGTATTGCAGCTAAaca < 1:2109697/68‑1 (MQ=255) tCCTATATGACTCTGGCGTATGGCAATGCACTGAAAGATAAGGTTATTGCTCGTATTGCAGCTAAaca < 1:1737558/68‑1 (MQ=255) tCCTATATGACTCTGGCGTATGGCAATGCACTGAAAGATAAGGTTATTGCTCGTATTGCAGCTAAaca < 1:2362900/68‑1 (MQ=255) tCCTATATGACTCTGGCGTATGGCAATGCACTGAAAGATAAGGTTATTGCTCGTATTGCAGCTAAaca < 1:2627024/68‑1 (MQ=255) tatGACGCTGGCGTATGGTAAGGCCCTGAAAGATGAGGTTATTGCTCGTATCGCAGCTAAacac < 1:2597360/64‑1 (MQ=255) tatGACGCTGGCGTATGGTAAGGCCCTGAAAGATGAGGTTATTGCTCGTATCGCAGCTAAACACAATg > 1:2415118/1‑68 (MQ=255) tatGACGCTGGCGTATGGTAAGGCCCTGAAAGATGAGGTTATTGCTCGTATCGCAGCTAAACACAATGc > 1:552153/1‑69 (MQ=255) tatGACGCTGGCGTATGGTAAGGCCCTGAAAGATGAGGTTATTGCTCGTATCGCAGCTAAACACAATGc > 1:1375014/1‑69 (MQ=255) tatGACGCTGGCGTATGGTAAGGCCCTGAAAGATGAGGTTATTGCTCGTATCGCAGCTAAACACAATGc > 1:1663353/1‑69 (MQ=255) tatGACGCTGGCGTATGGTAAGGCCCTGAAAGATGAGGTTATTGCTCGTATCGCAGCTAAACACAATGc > 1:2606984/1‑69 (MQ=255) tGACGCTGGCGTATGGTAAGGCCCTGAAAGATGAGGTTATTGCTCGTATCGCAGCTaaa < 1:630564/59‑1 (MQ=255) gTATGGTAAGGCCCTGAAAGATGAGGTTATTGCTCGTATCGCAGCTAAACACAATGCGACTCCGGcaca < 1:1441760/69‑1 (MQ=255) gTATGGTAAGGCCCTGAAAGATGAGGTTATTGCTCGTATCGCAGCTAAACACAATGCGACTCCGGcaca < 1:3212439/69‑1 (MQ=255) gTATGGTAAGGCCCTGAAAGATGAGGTTATTGCTCGTATCGCAGCTAAACACAATGCGACTCCGGcaca < 1:1082987/69‑1 (MQ=255) aGGCCCTGAAAGATGAGGTTATTGCTCGTATCGCAGCTAAACACAATGCGACTCCGGCACAAGTGATTc > 1:2383945/1‑69 (MQ=255) aGGCCCTGAAAGATGAGGTTATTGCTCGTATCGCAGCTAAACACAATGCGACTCCGGCACAAGTGATTc > 1:1674433/1‑69 (MQ=255) cTGAAAGATGAGGTTATTGCTCGTATCGCAGCTAAACACAATGCGACTCCGGCACAAGTGATTCTGGCg > 1:1261858/1‑69 (MQ=255) cTGAAAGATGAGGTTATTGCTCGTATCGCAGCTAAACACAATGCGACTCCGGCACAAGTGATTCTGGCg > 1:2706009/1‑69 (MQ=255) cTGAAAGATAAGGTTATTGCTCGTATTGCAGCTAAACACAATGCGACTCCGGCTCAAGTGATTCTGGCg < 1:91375/69‑1 (MQ=255) cTGAAAGATAAGGTTATTGCTCGTATTGCAGCTAAACACAATGCGACTCCGGCTCAAGTGATTCTGGCg < 1:1027493/69‑1 (MQ=255) cTGAAAGATAAGGTTATTGCTCGTATTGCAGCTAAACACAATGCGACTCCGGCTCAAGTGATTCTGGCg < 1:876586/69‑1 (MQ=255) cTGAAAGATAAGGTTATTGCTCGTATTGCAGCTAAACACAATGCGACTCCGGCTCAAGTGATTCTGGCg < 1:2300912/69‑1 (MQ=255) cTGAAAGATAAGGTTATTGCTCGTATTGCAGCTAAACACAATGCGACTCCGGCTCAAGTGATTCTGGCg < 1:1080711/69‑1 (MQ=255) tGAAAGATAAGGTTATTGCTCGTATTGCAGCTAAACACAATGCGACTCCGGCTCAAGTGATTCTGGCg < 1:864947/68‑1 (MQ=255) aGATGAGGTTATTGCTCGTATCGCAGCTAAACACAATGCGACTCCGGCACAAGTGATTCTGGCGTgg < 1:297059/67‑1 (MQ=255) aGATGAGGTTATTGCTCGTATCGCAGCTAAACACAATGCGACTCCGGCACAAGTGATTCTGGCGTgg < 1:2378600/67‑1 (MQ=255) aGATGAGGTTATTGCTCGTATCGCAGCTAAACACAATGCGACTCCGGCACAAGTGATTCTGGCGTgg < 1:2223739/67‑1 (MQ=255) aGATGAGGTTATTGCTCGTATCGCAGCTAAACACAATGCGACTCCGGCACAAGTGATTCTGGCGTgg < 1:2015316/67‑1 (MQ=255) aGATGAGGTTATTGCTCGTATCGCAGCTAAACACAATGCGACTCCGGCACAAGTGATTCTGGCGTgg < 1:1570975/67‑1 (MQ=255) tGAGGTTATTGCTCGTATCGCAGCTAAACACAATGCGACTCCGGCACAAGTGATTCTGGCGTGGGCTAt < 1:3299781/69‑1 (MQ=255) tGAGGTTATTGCTCGTATCGCAGCTAAACACAATGCGACTCCGGCACAAGTGATTCTGGCGTGGGCTAt < 1:2742195/69‑1 (MQ=255) tGAGGTTATTGCTCGTATCGCAGCTAAACACAATGCGACTCCGGCACAAGTGATTCTGGCGTGGGCTAt < 1:271161/69‑1 (MQ=255) tGAGGTTATTGCTCGTATCGCAGCTAAACACAATGCGACTCCGGCACAAGTGATTCTGGCGTGGGCTAt < 1:2538913/69‑1 (MQ=255) tGAGGTTATTGCTCGTATCGCAGCTAAACACAATGCGACTCCGGCACAAGTGATTCTGGCGTGGGCTAt < 1:1987090/69‑1 (MQ=255) tGAGGTTATTGCTCGTATCGCAGCTAAACACAATGCGACTCCGGCACAAGTGATTCTGGCGTGGGCTAt < 1:171100/69‑1 (MQ=255) tGAGGTTATTGCTCGTATCGCAGCTAAACACAATGCGACTCCGGCACAAGTGATTCTGGCGTGGGCTAt < 1:167051/69‑1 (MQ=255) tGAGGTTATTGCTCGTATCGCAGCTAAACACAATGCGACTCCGGCACAAGTGATTCTGGCGTGGGCTAt < 1:916283/69‑1 (MQ=255) | TAAACAGCACGGCATCCATATTACTTCCTATATGACGCTGGCGTATGGTAAGGCCCTGAAAGATGAGGTTATTGCTCGTATCGCAGCTAAACACAATGCGACTCCGGCACAAGTGATTCTGGCGTGGGCTAT > minE/208494‑208625 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |