| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,449,183 | G→A | 14.1% | D190N (GAT→AAT) | yifB → | predicted bifunctional protein, enzyme and transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,449,183 | 0 | G | A | 14.1% | 111.1 / 6.6 | 63 | D190N (GAT→AAT) | yifB | predicted bifunctional protein, enzyme and transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base G (22/32); new base A (4/5); total (26/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01 | |||||||||||
CGATCATCTGCAAGCTGTCTGTGCGTTTCTGGAAGGTAAGCACGCTCTCGAACGCCCGAAACCAACTGATGCAGTATCCCGGGCGCTACAACATGATCTCAGTGATGTTATCGGTCAGGAACAAGGAAAGCGAG > minE/2449116‑2449249 | cGATCATCTGCAAGCTGTCTGTGCGTTTCTGGAAGGTAAGCACGCTCTCGAACGCCCGAAACCAACTg > 1:17899/1‑68 (MQ=255)cGATCATCTGCAAGCTGTCTGTGCGTTTCTGGAAGGTAAGCACGCTCTCGAACGCCCGAAACCAACTGa > 1:1980186/1‑69 (MQ=255)cGATCATCTGCAAGCTGTCTGTGCGTTTCTGGAAGGTAAGCACGCTCTCGAACGCCCGAAACCAACTGa > 1:674143/1‑69 (MQ=255)cGATCATCTGCAAGCTGTCTGTGCGTTTCTGGAAGGTAAGCACGCTCTCGAACGCCCGAAACCAACTGa > 1:2251165/1‑69 (MQ=255)cGATCATCTGCAAGCTGTCTGTGCGTTTCTGGAAGGTAAGCACGCTCTCGAACGCCCGAAACCAACTGa > 1:2586502/1‑69 (MQ=255)cGATCATCTGCAAGCTGTCTGTGCGTTTCTGGAAGGTAAGCACGCTCTCGAACGCCCGAAACCAACTGa > 1:2918755/1‑69 (MQ=255) atcTGCAGGCTGTCTGCGCGTTTCTGGAAGGTAAGCACGCTCTCGAACACCCGAAACCAACTAATGCAg < 1:2537010/69‑1 (MQ=255) atcTGCAGGCTGTCTGCGCGTTTCTGGAAGGTAAGCACGCTCTCGAACACCCGAAACCAACTAATGCAg < 1:1299031/69‑1 (MQ=255) atcTGCAGGCTGTCTGCGCGTTTCTGGAAGGTAAGCACGCTCTCGAACACCCGAAACCAACTAATGCAg < 1:2928382/69‑1 (MQ=255) atcTGCAGGCTGTCTGCGCGTTTCTGGAAGGTAAGCACGCTCTCGAACACCCGAAACCAACTAATGCAg < 1:3057307/69‑1 (MQ=255) atcTGCAAGCTGTCTGTGCGTTTCTGGAAGGTAAGCACGCTATCGAACGCCCGAAACCAACTGATGCAg < 1:3109224/69‑1 (MQ=255) tcTGCAGGCTGTCTGCGCGTTTCTGGAAGGTAAGCACGCTCTCGAACACCCGAAACCAACTAATGCAg < 1:1299442/68‑1 (MQ=255) cAAGCTGTCTGTGCGTTTCTGGAAGGTAAGCACGCTCTCGAACGCCCGAAACCAACTGATGCAGTATcc > 1:2246788/1‑69 (MQ=255) tgtgCGTTTCTGGAAGGTAAGCACGCTCTCGAACGCCCGAAACCAACTGATGCAGTATCCCGGgcgc < 1:2165061/67‑1 (MQ=255) tgtgCGTTTCTGGAAGGTAAGCACGCTCTCGAACGCCCGAAACCAACTGATGCAGTATCCCGGgcgc < 1:1473579/67‑1 (MQ=255) cGTTTCTGGAAGGTAAGCACGCTCTCTAACGCCCGAAACCAACTGATGCAg < 1:2078615/51‑1 (MQ=255) cGTTTCTGGAAGGTAAGCACGCTCTCGAACGCCCGAATCCAACTGATGCAg < 1:2858125/51‑1 (MQ=255) cGTTTCTGGAAGGTAAGCACGCTCTCGAACGCCCGAAACCAACTGATGCAg < 1:1609168/51‑1 (MQ=255) cGTTTCTGGAAGGTAAGCACGCTCTCGAACGCCCGAAACCAACTGATGCAg > 1:1631100/1‑51 (MQ=255) cGTTTCTGGAAGGTAAGCACGCTCTCGAACGCCCGAAACCAACTGATGCAg > 1:3230825/1‑51 (MQ=255) cGTTTCTGGAAGGTAAGCACGCTCTCGAACGCCCGAAACCAACTGATGCAg < 1:412390/51‑1 (MQ=255) cGTTTCTGGAAGGTAAGCACGCTCTCGAACGCCCGAAACCAACTGATGCAg > 1:590504/1‑51 (MQ=255) cGTTTCTGGAAGGTAAGCACGCTCTCGAACGCCCGAAACCAACTGATGCAg < 1:2663206/51‑1 (MQ=255) cGTTTCTGGAAGGTAAGCACGCTCTCGAACGCCCGAAACCAACTGATGCAg < 1:612463/51‑1 (MQ=255) cGTTTCTGGAAGGTAAGCACGCTCTCGAACGCCCGAAACCAACTGATGCAg < 1:2497355/51‑1 (MQ=255) tCTGGAAGTTAAGCACGCTCTCGAACACCCGAAACCAACTAATGCAGTATCCCGGGCGCTACAACATGa > 1:3361057/1‑69 (MQ=255) tCTGGAAGGTAAGCACGCTCTCGAACACCCGAAACCAACTAATGCAGTATCCCGGGCGCTACAACATGa > 1:3177122/1‑69 (MQ=255) tCTGGAAGGTAAGCACGCTCTCGAACACCCGAAACCAACTAATGCAGTATCCCGGGCGCTACAACATGa > 1:1494341/1‑69 (MQ=255) tCTGGAAGGTAAGCACGCTCTCGAACACCCGAAACCAACTAATGCAGTATCCCGGGCGCTACAACATGa > 1:1228663/1‑69 (MQ=255) ggTAAGCACGCTCTCGAACGCCCGATACCAACTGATGCAGTATCCCGGGCGCTACAACATGATCTCAGt < 1:665338/69‑1 (MQ=255) ggTAAGCACGCTCTCGAACGCCCGAAACCAACTGATGCAGTATCCCGGGCGCTACAACATGATCTCAGt < 1:1906360/69‑1 (MQ=255) ggTAAGCACGCTCTCGAACGCCCGAAACCAACTGATGCAGTATCCCGGGCGCTACAACATGATCTCAGt < 1:3194067/69‑1 (MQ=255) ggTAAGCACGCTCTCGAACGCCCGAAACCAACTGATGCAGTATCCCGGGCGCTACAACATGATCTCAGt < 1:11116/69‑1 (MQ=255) ggTAAGCACGCTCTCGAACGCCCGAAACCAACTGATGCAGTATCCCGGGCGCTACAACATGATCTCAGt < 1:1160450/69‑1 (MQ=255) ggTAAGCACGCTCTCGAACGCCCGAAACCAACTGATGCAGTATACCGGGCGCTACAACATGATCTCAGt < 1:2326511/69‑1 (MQ=255) ggTAAGCACGCTCTCGAACGCCCGAAACCAACTGACGCAGTATCCCGGGCGCTACAACATGATCTCAGt < 1:1222405/69‑1 (MQ=255) ggTAAGCACGCTCTCGAACGCCCGAAAACAACTGATGCAGTATCCCGGGCGCTACAACATGATCTCAGt < 1:2556804/69‑1 (MQ=255) gTAAGCACGCTCTCGAACGCCCGAAACCAACTGATGCAGTATCCCGGGCGCTACAACATGATCTCAGt < 1:1788888/68‑1 (MQ=255) tAAGCACGCTCTCGAACGCCCGAAACCAACTGATGCAGTATCCCGGGCGCTACAACATGATCTCAGTGa > 1:96538/1‑69 (MQ=255) tAAGCACGCTCTCGAACGCCCGAAACCAACTGATGCAGTATCCCGGGCGCTACAACATGATCTCAGTGa > 1:879272/1‑69 (MQ=255) tAAGCACGCTCTCGAACGCCCGAAACCAACTGATGCAGTATCCCGGGCGCTACAACATGATCTCAGTGa > 1:818564/1‑69 (MQ=255) tAAGCACGCTCTCGAACGCCCGAAACCAACTGATGCAGTATCCCGGGCGCTACAACATGATCTCAGTGa > 1:1483174/1‑69 (MQ=255) tAAGCACGCTCTCGAACGCCCGAAACCAACTGATGCAGTATCCCGGGCGCTACAACATGATCTCAGTGa > 1:1840727/1‑69 (MQ=255) aaGCACGCTCTCGAACGCCCGAAACCAACTGATGCAGTATCCCGGGCGCTACAACATGATCTCAGTGAt < 1:2368894/69‑1 (MQ=255) cACGCTCTCGAACGCCCGAACCCAACTGATGCAGTATCCCGGGCGCTACAACATGATCTCAGTGATGtt > 1:2658903/1‑69 (MQ=255) cACGCTCTCGAACGCCCGAAACCAACTGATGCAGTATCCCGGgcgc > 1:121899/1‑46 (MQ=255) cACGCTCTCGAACGCCCGAAACCAACTGATGCAGTATCCCGGGCGCTACAACATGATCTCAGTGATGtt > 1:2570388/1‑69 (MQ=255) cACGCTCTCGAACGCCCGAAACCAACTGATGCAGTATCCCGGGCGCTACAACATGATCTCAGTGATGtt > 1:2937750/1‑69 (MQ=255) cACGCTCTCGAACGCCCGAAACCAACTGATGCAGTATCCCGGGCGCTACAACATGATCTCAGTGATGtt > 1:3059592/1‑69 (MQ=255) cACGCTCTCGAACGCCCGAAACCAACTGATGCAGTATCCCGGGCGCTACAACATGATCTCAGTGATGtt > 1:1396788/1‑69 (MQ=255) cACGCTCTCGAACGCCCGAAACCAACTGATGCAGTATCCAGGGCGCTAAAACATGATCTCAGTGATGtt > 1:1782639/1‑69 (MQ=255) aCGCTCTCGAACGCCCGAAACCAACTGATGCAGTATCCCGGGCGCTACAACATGATCTCAGTGATGTTa < 1:1982651/69‑1 (MQ=255) aCGCTCTCGAACGCCCGAAACCAACTGATGCAGTATCCCGGGCGCTACAACATGATCTCAGTGATGTTa < 1:1566981/69‑1 (MQ=255) aaCCAACTGATGCAGTATCCCGGGCGCTACAACATGATCTCAGTGATGTTATCGGTCAGGAACAAGGaa < 1:1489544/69‑1 (MQ=255) cTGATGCAGTATCCCGGGCGCTACAACATGATCTCAGTGATGTTATCGGTCAGGAACAAGGAAAGCGAg < 1:2472533/69‑1 (MQ=255) cTGATGCAGTATCCCGGGCGCTACAACATGATCTCAGTGATGTTATCGGTCAGGAACAAGGAAAGCGAg < 1:183824/69‑1 (MQ=255) cTGATGCAGTATCCCGGGCGCTACAACATGATCTCAGTGATGTTATCGGTCAGGAACAAGGAAAGCGAg < 1:2357125/69‑1 (MQ=255) cTGATGCAGTATCCCGGGCGCTACAACATGATCTCAGTGATGTTATCGGTCAGGAACAAGGAAAGCGAg > 1:1281545/1‑69 (MQ=255) cTGATGCAGTATCCCGGGCGCTACAACATGATCTCAGTGATGTTATCGGTCAGGAACAAGGAAAGCGAg < 1:1378014/69‑1 (MQ=255) cTGATGCAGTATCCCGGGCGCTACAACATGATCTCAGTGATGTTATCGGTCAGGAACAAGGAAAGCGAg < 1:1634217/69‑1 (MQ=255) cTGATGCAGTATCCCGGGCGCTACAACATGATCTCAGTGATGTTATCGGTCAGGAACAAGGAAAGCGAg < 1:1693596/69‑1 (MQ=255) cTGATGCAGTATCCCGGGCGCTACAACATGATCTCAGTGATGTTATCGGTCAGGAACAAGGAAAGCGAg < 1:2779690/69‑1 (MQ=255) cTGATGCAGTATCCCGGGCGCTACAACATGATCTCAGTGATGTTATCGGTCAGGAACAAGGAAAGCGAg < 1:1832340/69‑1 (MQ=255) cTGATGCAGTATCCCGGGCGCTACAACATGATCTCAGTGATGTTATCGGTCAGGAACAAGGAAAGCGAg < 1:2913066/69‑1 (MQ=255) | CGATCATCTGCAAGCTGTCTGTGCGTTTCTGGAAGGTAAGCACGCTCTCGAACGCCCGAAACCAACTGATGCAGTATCCCGGGCGCTACAACATGATCTCAGTGATGTTATCGGTCAGGAACAAGGAAAGCGAG > minE/2449116‑2449249 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |