| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,449,225 | A→G | 19.6% | I204V (ATC→GTC) | yifB → | predicted bifunctional protein, enzyme and transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,449,225 | 0 | A | G | 19.6% | 94.7 / 15.0 | 66 | I204V (ATC→GTC) | yifB | predicted bifunctional protein, enzyme and transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base A (16/37); new base G (11/2); total (27/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.04e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACGCTCTCGAACGCCCGAAACCAACTGATGCAGTATCCCGGGCGCTACAACATGATCTCAGTGATGTTATCGGTCAGGAACAAGGAAAGCGAGGACTGGAAATTACCGCTGCTGGCGGGCACAACCTTTTACTGATT > minE/2449157‑2449293 | aCGCTCTCGAACGCCCGAAACCAACTGATGCAGTATCCCGGGCGCTACAACATGATCTCAGTGATGTTa < 1:1982651/69‑1 (MQ=255)aCGCTCTCGAACGCCCGAAACCAACTGATGCAGTATCCCGGGCGCTACAACATGATCTCAGTGATGTTa < 1:1566981/69‑1 (MQ=255) aaCCAACTGATGCAGTATCCCGGGCGCTACAACATGATCTCAGTGATGTTATCGGTCAGGAACAAGGaa < 1:1489544/69‑1 (MQ=255) cTGATGCAGTATCCCGGGCGCTACAACATGATCTCAGTGATGTTATCGGTCAGGAACAAGGAAAGCGAg < 1:1832340/69‑1 (MQ=255) cTGATGCAGTATCCCGGGCGCTACAACATGATCTCAGTGATGTTATCGGTCAGGAACAAGGAAAGCGAg < 1:1693596/69‑1 (MQ=255) cTGATGCAGTATCCCGGGCGCTACAACATGATCTCAGTGATGTTATCGGTCAGGAACAAGGAAAGCGAg < 1:2357125/69‑1 (MQ=255) cTGATGCAGTATCCCGGGCGCTACAACATGATCTCAGTGATGTTATCGGTCAGGAACAAGGAAAGCGAg < 1:1634217/69‑1 (MQ=255) cTGATGCAGTATCCCGGGCGCTACAACATGATCTCAGTGATGTTATCGGTCAGGAACAAGGAAAGCGAg < 1:2472533/69‑1 (MQ=255) cTGATGCAGTATCCCGGGCGCTACAACATGATCTCAGTGATGTTATCGGTCAGGAACAAGGAAAGCGAg < 1:2779690/69‑1 (MQ=255) cTGATGCAGTATCCCGGGCGCTACAACATGATCTCAGTGATGTTATCGGTCAGGAACAAGGAAAGCGAg < 1:1378014/69‑1 (MQ=255) cTGATGCAGTATCCCGGGCGCTACAACATGATCTCAGTGATGTTATCGGTCAGGAACAAGGAAAGCGAg < 1:2913066/69‑1 (MQ=255) cTGATGCAGTATCCCGGGCGCTACAACATGATCTCAGTGATGTTATCGGTCAGGAACAAGGAAAGCGAg < 1:183824/69‑1 (MQ=255) cTGATGCAGTATCCCGGGCGCTACAACATGATCTCAGTGATGTTATCGGTCAGGAACAAGGAAAGCGAg > 1:1281545/1‑69 (MQ=255) taaTGGAGTATCCGGGGCGCTACAACATGATCTCAGTGATGTTGTCGGTCAGGAGCAAGGAAAGCGAgg > 1:1219441/3‑69 (MQ=255) taaTGCAGTATCCCGGGCGCTACAACATGATCTCAGTGATGTTGTCGGTCAGGAGCAAGGAAAGCGAgg > 1:1939207/3‑69 (MQ=255) taaTGCAGTATCCCGGGCGCTACAACATGATCTCAGTGATGTTGTCGGTCAGGAGCAAGGAAAGCGAgg > 1:1982254/3‑69 (MQ=255) taaTGCAGTATCCCGGGCGCTACAACATGATCTCAGTGATGTTGTCGGTCAGGAGCAAGGAAAGCGAgg > 1:610277/3‑69 (MQ=255) taaTGCAGTATCCCGGGCGCTACAACATGATCTCAGTGATGTTGTCGGTCAGGAGCAAGGAAAGCGAgg > 1:2314702/3‑69 (MQ=255) taaTGCAGTATCCCGGGCGCTACAACATGATCTCAGTGATGTTGTCGGTCAGGAGCAAGGAAAGCGAgg > 1:240872/3‑69 (MQ=255) taaTGCAGTATCCCGGGCGCTACAACATGATCTCAGTGATGTTGTCGGTCAGGAGCAAGGAAAGCGAgg > 1:2899712/3‑69 (MQ=255) taaTGCAGTATCCCGGGCGCTACAACATGATCTCAGTGATGTTGTCGGTCAGGAGCAAGGAAAGCGAgg > 1:2941252/3‑69 (MQ=255) taaTGCAGTATCCCGGGCGCTACAACATGATCTCAGTGATGTTGTCGGTCAGGAGCAAGGAAAGCGAgg > 1:365019/3‑69 (MQ=255) taaTGCAGTATCCCGGGCGCTACAACATGATCTCAGTGATGTTGTCGGTCAGGAGCAAGGAAAGCGAgg > 1:386263/3‑69 (MQ=255) taaTGCAGTATCCCGGGCGCTACAACATGATCTCAGTGATGTTGTCGGTCAGGAGCAAGGAAAGCGAgg > 1:1454070/3‑69 (MQ=255) gTATCCCTGGCGCTACAACATGATCTCAGTGATGTTATCGGTCAGGAACAAGGAAAGCGAGGACTGGa < 1:2986567/68‑1 (MQ=255) gTATCCCGGGCGCTACAACATGATCTCAGTGATGTTATCGGTCAGGAACAAGGAAAGCGAGGACTGGa < 1:2965946/68‑1 (MQ=255) gTATCCCGGGCGCTACAACATGATCTCAGTGATGTTATCGGTCAGGAACAAGGAAAGCGAGGACTGGa < 1:1628894/68‑1 (MQ=255) gTATCCCGGGCGCTACAACATGATCTCAGTGATGTTATCGGTCAGGAACAAGGAAAGCGAGGACTGGa < 1:382884/68‑1 (MQ=255) gTATCCCGGGCGCTACAACATGATCTCAGTGATGTTATCGGTCAGGAACAAGGAAAGCGAGGACTGGa < 1:1669526/68‑1 (MQ=255) gTATCCCGGGCGCTACAACATGATCTCAGTGATGTTATCGGTCAGGAACAAGGAAAGCGAGGACTGGa < 1:1190009/68‑1 (MQ=255) gTATCCCGGGCGCTACAACATGATCTCAGTGATGTTATCGGTCAGGAACAAGGAAAGCGAGGACTGGa < 1:3125507/68‑1 (MQ=255) aTCCCGGGCGCTACAACATGATCTCAGTGATGTTATCGGTCAGGAACAAGGaa > 1:2392245/1‑53 (MQ=255) tCCCGGGCGCTACAACATGATCTCAGTGATGTTATCGGTCAGGAACAAGGAAAGCGAGGACTGGAAAtt > 1:402225/1‑69 (MQ=255) tCCCGGGCGCTACAACATGATCTCAGTGATGTTATCGGTCAGGAACAAGGAAAGCGAGGACTGGAAAtt > 1:453375/1‑69 (MQ=255) tCCCGGGCGCTACAACATGATCTCAGTGATGTTATCGGTCAGGAACAAGGAAAGCGAGGACTGGAAAtt > 1:1900569/1‑69 (MQ=255) ccGGGCGCTACAACATGATCTCAGTGATGTTATCGGTCAGGAACAAGGAAAGCGAGGACTGGAAATTAc > 1:2744156/1‑69 (MQ=255) ccGGGCGCTACAACATGATCTCAGTGATGTTATCGGTCAGGAACAAGGAAAGCGAGGACTGGAAATTAc > 1:835885/1‑69 (MQ=255) ggCGCTACAACATGATCTCAGTGATGTTATCGGTCAGGAACAAGGAAAGCGAGGACTGGAAATTACCgc > 1:312495/1‑69 (MQ=255) ggCGCTACAACATGATCTCAGTGATGTTATCGGTCAGGAACAAGGAAAGCGAGGACTGGAAATTACCgc > 1:247327/1‑69 (MQ=255) gATCTCAGTGATGTTGTCGGTCAGGAGCAAGGAAAGCGAGGACTGGAAATTACCGCCGCTGGCGGGcac < 1:3403622/69‑1 (MQ=255) gATCTCAGTGATGTTGTCGGTCAGGAGCAAGGAAAGCGAGGACTGGAAATTACCGCCGCTGGCGGGcac < 1:1823179/69‑1 (MQ=255) aTCTCAGTGATGTTATCGGTCAGGAACAAGGAAAGCGAGGACTGGAAATTACCGCTGCTGGCGGGcaca < 1:184523/69‑1 (MQ=255) aTCTCAGTGATGTTATCGGTCAGGAACAAGGAAAGCGAGGACTGGAAATTACCGCTGCTGGCGGGcaca > 1:1295169/1‑69 (MQ=255) aTCTCAGTGATGTTATCGGGCAGGAACAAGGAAAGCGAGGACTGGAAATTACCGCTGCTGGCGGGcaca < 1:2927343/69‑1 (MQ=255) aGTGATGTTATCGGTCAGGAACAAGGAAAGCGAGGACTGGAAATTACCGCTGCTGGCGGGcaca < 1:621709/64‑1 (MQ=255) tGATGTTATCGGTCAGGAACAAGGAAAGCGAGGACTGGAAATTACCGCTGCTGGCGGGCACAACCttt < 1:2761995/68‑1 (MQ=255) tGATGTTATCGGTCAGGAACAAGGAAAGCGAGGACTGGAAATTACCGCTGCTGGCGGGCACAACCttt < 1:2694696/68‑1 (MQ=255) tGATGTTATCGGTCAGGAACAAGGAAAGCGAGGACTGGAAATTACCGCTGCTGGCGGGCACAACCttt < 1:858766/68‑1 (MQ=255) gATGTTATCGGTCAGGAACAAGGAAAGCGAGGACTGGAAATTACCGCTGCTGGCGGGCACAACCttt < 1:1622984/67‑1 (MQ=255) aTGTTATCGGTCAGGAACAAGGAAAGCGAGGACTGGAAATTACCGCTGCTGGCGGGCACAACCTTTTa > 1:2959060/1‑68 (MQ=255) aTGTTATCGGTCAGGAACAAGGAAAGCGAGGACTGGAAATTACCGCTGCTGGCGGGCACAACCTTTTa > 1:2655532/1‑68 (MQ=255) aTGTTATCGGTCAGGAACAAGGAAAGCGAGGACTGGAAATTACCGCTGCTGGCGGGCACAACCTTTTa > 1:3252177/1‑68 (MQ=255) aTGTTATCGGTCAGGAACAAGGAAAGCGAGGACTGGAAATTACCGCTGCTGGCGGGCACAACCTTTTa > 1:2379630/1‑68 (MQ=255) aTGTTATCGGTCAGGAACAAGGAAAGCGAGGACTGGAAATTACCGCTGCTGGCGGGCACAACCTTTTa > 1:3147232/1‑68 (MQ=255) gTTATCGGTCAGGAACAAGGAAAGCGAGGACTGGaaa > 1:294686/1‑37 (MQ=255) gTTATCGGTCAGGAACAAGGAAAGCGAGGACTGGAAATTACCGCTGCTGGCGGGCACAACCttt < 1:1636780/64‑1 (MQ=255) gTTATCGGTCAGGAACAAGGAAAGCGAGGACTGGAAATTACCGCTGCTGGCGGGCACAACCttt < 1:3283280/64‑1 (MQ=255) gTTATCGGTCAGGAACAAGGAAAGCGAGGACTGGAAATTACCGCTGCTGGCGGGCACAACCttt < 1:397725/64‑1 (MQ=255) gTTATCGGTCAGGAACAAGGAAAGCGAGGACTGGAAATTACCGCTGCTGGCGGGCACAACCttt < 1:3030291/64‑1 (MQ=255) gTTATCGGTCAGGAACAAGGAAAGCGAGGACTGGAAATTACCGCTGCTGGCGGGCACAACCttt < 1:1012154/64‑1 (MQ=255) gTTATCGGTCAGGAACAAGGAAAGCGAGGACTGGAAATTACCGCTGCTGGCGGGCACAACCttt < 1:27551/64‑1 (MQ=255) ttATCGGTCAGGAACAAGGAAAGCGAGGACTGGAAATTACCGCTGCTGGCGGGCACAACCttt < 1:2124729/63‑1 (MQ=255) ttATCGGTCAGGAACAAGGAAAGCGAGGACTGGAAATTACCGCTGCTGGCGGGCACAACCttt < 1:2185835/63‑1 (MQ=255) ttATCGGTCAGGAACAAGGAAAGCGAGGACTGGAAATTACCGCTGCTGGCGGGCACAACCttt < 1:1614186/63‑1 (MQ=255) ttATCGGTCAGGAACAAGGAAAGCGAGGACTGGAAATTACCGCTGCTGGCGGGCACAACCttt < 1:359806/63‑1 (MQ=255) ttATCGGTCAGGAACAAGGAAAGCGAGGACTGGAAATTACCGCTGCTGGCGGGCACAACCttt < 1:3248986/63‑1 (MQ=255) ttATCGGTCAGGAACAAGGAAAGCGAGGACTGGAAATTACCGCTGCTGGCGGGCACAACCttt < 1:3161025/63‑1 (MQ=255) ttATCGGTCAGGAACAAGGAAAGCGAGGACTGGAAATTACCGCTGCTGGCGGGCACAACCttt < 1:2751905/63‑1 (MQ=255) aTCGGTCAGGAACAAGGAAAGCGAGGACTGGAAATTACCGCTGCTGGCGGGCACAACCTTTTACTGAtt > 1:827088/1‑69 (MQ=255) aTCGGTCAGGAACAAGGAAAGCGAGGACTGGAAATTACCGCTGCTGGCGGGCACAACCTTTTACTGAtt > 1:2520094/1‑69 (MQ=255) aTCGGTCAGGAACAAGGAAAGCGAGGACTGGAAATTACCGCTGCTGGCGGGCACAACCTTTTACTGAtt > 1:2371566/1‑69 (MQ=255) aTCGGTCAGGAACAAGGAAAGCGAGGACTGGAAATTACCGCTGCTGGCGGGCACAACCTTTTACTGAtt > 1:214785/1‑69 (MQ=255) aTCGGTCAGGAACAAGGAAAGCGAGGACTGGAAATTACCGCTGCTGGCGGGCACAACCTTTTACTGAtt > 1:1318171/1‑69 (MQ=255) aTCGGTCAGGAACAAGGAAAGCGAGGACTGGAAATTACCGCTGCTGGCGGGCACAACCTTTTACTGAtt > 1:1035586/1‑69 (MQ=255) | ACGCTCTCGAACGCCCGAAACCAACTGATGCAGTATCCCGGGCGCTACAACATGATCTCAGTGATGTTATCGGTCAGGAACAAGGAAAGCGAGGACTGGAAATTACCGCTGCTGGCGGGCACAACCTTTTACTGATT > minE/2449157‑2449293 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |