| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,451,169 | A→T | 53.0% | S174S (TCA→TCT) | hdfR → | DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,451,169 | 0 | A | T | 53.0% | 1.3 / 54.6 | 49 | S174S (TCA→TCT) | hdfR | DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base A (6/17); new base T (11/15); total (17/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.67e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01 | |||||||||||
GCTGGGATATTTCACTTTAGCGCTTTATACCAGTGCCCCTTCAAAACTAAAGGGAGATCTTAATTATCTGCGACTTGAGTGGGGGCCAG > minE/2451127‑2451215 | gCTGGGATATTTCACTTTAGCGCTTTATACCAGTGCCCCTTCAAAACTAAAGGGAGATCTTAATTATCt > 1:15234/1‑69 (MQ=255) atTTCACTTTAGCGCTTTATACCAGTGCCCCTTCAAAACTAAAGGGAGATCTTAATTATCTGCGACTTg < 1:976547/69‑1 (MQ=255) atTTCACTTTAGCGCTTTATACCAGTGCCCCTTCAAAACTAAAGGGAGATCTTAATTATCTGCGACTTg < 1:2156648/69‑1 (MQ=255) atTTCACTTTAGCGCTTTATACCAGTGCCCCTTCAAAACTAAAGGGAGATCTTAATTATCTGCGACTTg < 1:3411961/69‑1 (MQ=255) tttCTCTTTAGCGCTTTATACCAGTGCCCCTTCAAAACTAAAGGGAGATCTTAATTATCTGCGACTTg > 1:206174/1‑68 (MQ=255) tttCACTTTAGCGCTTTATACCAGTGCCCCTTCAAAACTAAAGGGAGATCTTAATTATCTGCGACTTg < 1:457528/68‑1 (MQ=255) tttCACTTTAGCGCTTTATACCAGTGCCCCTTCAAAACTAAAGGGAGATCTTAATTATCTGCGACTTg < 1:3127889/68‑1 (MQ=255) tttCACTTTAGCGCTTTATACCAGTGCCCCTTCAAAACTAAAGGGAGATCTTAATTATCTGCGACTTg < 1:2221305/68‑1 (MQ=255) tttCACTTTAGCGCTTTATACCAGTGCCCCTTCAAAACTAAAGGGAGATCTTAATTATCTGCGACTTg < 1:1710124/68‑1 (MQ=255) tttCACTTTAGCGCTTTATACCAGTGCCCCTTCAAAACTAAAGGGAGATCTTAATTATCTGCGACTTg > 1:1507101/1‑68 (MQ=255) tttCACTTTAGCGCTTTATACCAGTGCCCCTTCAAAACTAAAGGGAGATCTTAATTATCTGCGACTTg > 1:1465173/1‑68 (MQ=255) tttCACTTTAGCGCTTTATACCAGTGCCCCTTCAAAACTAAAGGGAGATCTTAATTATCTGCGACTTg > 1:75692/1‑68 (MQ=255) tttCACTTTAGCGCTTTATACCAGTGCCCCTTCAAAACTAAAGGGAGATCTTAATTATCTGCGACTTg < 1:922718/68‑1 (MQ=255) cACTTTAGCGCTTTATACCAGTGCCCCTTCAAAACTAAAGGGAGATCTTAATTATCTGCGACTTGAGTg > 1:55826/1‑69 (MQ=255) aCTTTAGCGCTTTATACCAGTGGCCCTTCTAAACTAAAGGGAGATCTTAACTATCTGCGACTAGAGTgg > 1:1685611/1‑69 (MQ=255) aCTTTAGCGCTTTATACCAGTGCCCCTTCTAAACTAAAggg > 1:3411513/1‑41 (MQ=255) aCTTTAGCGCTTTATACCAGTGCCCCTTCTAAACTAAAGGGAGATCTTAACTATCTGCGACTAGAGTgg > 1:3053711/1‑69 (MQ=255) aCTTTAGCGCTTTATACCAGTGCCCCTTCTAAACTAAAGGGAGATCTTAACTATCTGCGACTAGAGTgg > 1:90824/1‑69 (MQ=255) aCTTTAGCGCTTTATACCAGTGCCCCTTCTAAACTAAAGGGAGATCTTAACTATCTGCGACTAGAGTgg > 1:3371218/1‑69 (MQ=255) aCTTTAGCGCTTTATACCAGTGCCCCTTCTAAACTAAAGGGAGATCTTAACTATCTGCGACTAGAGTgg > 1:971105/1‑69 (MQ=255) aCTTTAGCGCTTTATACCAGTGCCCCTTCTAAACTAAAGGGAGATCTTAACTATCTGCGACTAGAGTgg > 1:2853748/1‑69 (MQ=255) aCTTTAGCGCTTTATACCAGTGCCCCTTCTAAACTAAAGGGAGATCTTAACTATCTGCGACTAGAGTgg > 1:1172489/1‑69 (MQ=255) aCTTTAGCGCTTTATACCAGTGCCCCTTCTAAACTAAAGGGAGATCTTAACTATCTGCGACTAGAGTgg > 1:1582342/1‑69 (MQ=255) aCTTTAGCGCTTTATACCAGTGCCCCTTCTAAACTAAAGGGAGATCTTAACTATCTGCGACTAGAGTgg > 1:2182877/1‑69 (MQ=255) aCTTTAGCGCTTTATACCAGTGCCCCTTCTAAACTAAAGGGAGATCTTAACTATCTGCGACTAGAGTg > 1:242975/1‑68 (MQ=255) gcTTTATACCAGTGCCCCTTCAAAACTAAAGGGAGATCTTAATTATCTGCGACTTGAGTGGGGGCCAg < 1:2641164/68‑1 (MQ=255) gcTTTATACCAGTGCCCCTTCAAAACTAAAGGGAGATCTTAATTATCTGCGACTTGAGTGGGGGCCAg < 1:747966/68‑1 (MQ=255) gcTTTATACCAGTGCCCCTTCAAAACTAAAGGGAGATCTTAATTATCTGCGACTTGAGTGGGGGCCAg < 1:717934/68‑1 (MQ=255) gcTTTATACCAGTGCCCCTTCAAAACTAAAGGGAGATCTTAATTATCTGCGACTTGAGTGGGGGCCAg < 1:1529888/68‑1 (MQ=255) gcTTTATACCAGTGCCCCTTCAAAACTAAAGGGAGATCTTAATTATCTGCGACTTGAGTGGGGGCCAg < 1:829004/68‑1 (MQ=255) gcTTTATACCAGTGCCCCTTCAAAACTAAAGGGAGATCTTAATTATCTGCGACTTGAGTGGGGGCCAg < 1:358810/68‑1 (MQ=255) gcTTTATACCAGTGCCCCTTCAAAACTAAAGGGAGATCTTAATTATCTGCGACTTGAGTGGGGGCCAg < 1:3146717/68‑1 (MQ=255) gcTTTATACCAGTGCCCCTTCAAAACTAAAGGGAGATCTTAATTATCTGCGACTTGAGTGGGGGCCAg < 1:2436087/68‑1 (MQ=255) tttATACCAGTGCCCCTTCAAAACTAAAGGGAGATCTTAATTATCTGCGACTTg < 1:3391371/54‑1 (MQ=255) gTGCCCCTTCTAAACTAAAGGGAGATCTTAACTATCTGCGACtag < 1:1200516/45‑3 (MQ=255) gTGCCCCTTCTAAACTAAAGGGAGATCTTAACTATCTGCGACtag < 1:836912/45‑3 (MQ=255) gTGCCCCTTCTAAACTAAAGGGAGATCTTAACTATCTGCGACtag < 1:1210886/45‑3 (MQ=255) gTGCCCCTTCTAAACTAAAGGGAGATCTTAACTATCTGCGACtag < 1:1179575/45‑3 (MQ=255) gTGCCCCTTCTAAACTAAAGGGAGATCTTAACTATCTGCGACtag < 1:1135393/45‑3 (MQ=255) gTGCCCCTTCTAAACTAAAGGGAGATCTTAACTATCTGCGACtag < 1:73926/45‑3 (MQ=255) gTGCCCCTTCTAAACTAAAGGGAGATCTTAACTATCTGCGACtag < 1:719980/45‑3 (MQ=255) gTGCCCCTTCTAAACTAAAGGGAGATCTTAACTATCTGCGACtag < 1:631109/45‑3 (MQ=255) gTGCCCCTTCTAAACTAAAGGGAGATCTTAACTATCTGCGACtag < 1:402552/45‑3 (MQ=255) gTGCCCCTTCTAAACTAAAGGGAGATCTTAACTATCTGCGACtag < 1:2145627/45‑3 (MQ=255) gTGCCCCTTCTAAACTAAAGGGAGATCTTAACTATCTGCGACtag < 1:1052952/45‑3 (MQ=255) gTGCCCCTTCTAAACTAAAGGGAGATCTTAACTATCTGCGACtag < 1:295620/45‑3 (MQ=255) gTGCCCCTTCTAAACTAAAGGGAGATCTTAACTATCTGCGACtag < 1:250233/45‑3 (MQ=255) gTGCCCCTTCTAAACTAAAGGGAGATCTTAACTATCTGCGACt < 1:435052/43‑1 (MQ=38) tGCCCCTTCTAAACTAAAGGGAGATCTTAACTATCTGCGACtag < 1:83788/44‑3 (MQ=38) | GCTGGGATATTTCACTTTAGCGCTTTATACCAGTGCCCCTTCAAAACTAAAGGGAGATCTTAATTATCTGCGACTTGAGTGGGGGCCAG > minE/2451127‑2451215 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |