| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,457,681 | A→G | 30.3% | K131K (AAA→AAG) | yieP → | predicted transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,457,681 | 0 | A | G | 30.3% | 92.4 / 48.6 | 102 | K131K (AAA→AAG) | yieP | predicted transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base A (39/32); new base G (16/15); total (55/47) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.30e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.89e-01 | |||||||||||
Only 100 of 107 total aligned reads displayed.
ATGCGTATCTGTCTGGAGCCGCAAGCCTGCCTACTGGCAGCAACGGTTGGCACCGCGGAACAGAAAGCGCATCTCAATACGTTAATGGCCGAAATGGCGGCATTAAAAGAGAACTTTCGCCGTGAACGC > minE/2457616‑2457744 | aTGCGTATCTGTCTGGAGCCGCAAGCCTGCCTACTGGCAGCAACGGTTGGCACCGCGGAACAGAAAgcg > 1:2174837/1‑69 (MQ=255)aTGCGTATCTGTCTGGAGCCGCAAGCCTGCCTACTGGCAGCAACGGTTGGCACCGCGGAACAGAAAgcg > 1:1859645/1‑69 (MQ=255)aTGCGTATCTGTCTGGAGCCGCAAGCCTGCCTACTGGCAGCAACGGTTGGCACCGCGGAACAGAAAgcg > 1:1305897/1‑69 (MQ=255)aTGCGTATCTGTCTGGAGCCGCAAGCCTGCCTACTGGCAGCAACGGTTGGCACCGCGGAACAGAAAgcg > 1:1661150/1‑69 (MQ=255)aTGCGTATCTGTCTGGAGCCGCAAGCCTGCCTACTGGCAGCAACGGTTGGCACCGCGGAACAGAAAgcg > 1:1794890/1‑69 (MQ=255)aTGCGTATCTGTCTGGAGCCGCAAGCCTGCCTACTGGCAGCAACGGTTGGCACCGCGGAACAGAAAgc > 1:182966/1‑68 (MQ=255) cGTATCTGTCTGGAGCCGCAAGCCTGCCTACTGGCAGCAACGGTTGGCACCGCGGAACAGAAAGCGCa > 1:2175673/1‑68 (MQ=255) cGTATCTGTCTGGAGCCGCAAGCCTGCCTACTGGCAGCAACGGTTGGCACCGCGGAACAGAAAGCGCa > 1:2897302/1‑68 (MQ=255) cGTATCTGTCTGGAGCCGCAAGCCTGCCTACTGGCAGCAACGGTTGGCACCGCGGAACAGAAAGCGCAt > 1:2798793/1‑69 (MQ=255) cGTATCTGTCTGGAGCCGCAAGCCTGCCTACTGGCAGCAACGGTTGGCACCGCGGAACAGAAAGCGCAt > 1:879170/1‑69 (MQ=255) cGTATCTGTCTGGAGCCGCAAGCCTGCCTACTGGCAGCAACGGTTGGCACCGCGGAACAGAAAGCGCAt > 1:881389/1‑69 (MQ=255) aTCTGTCTGGAGCCGCAAGCCTGCCTACTGGCAGCAACGGTTGGCACCGCGGAACAGAAAGCGCAtctc < 1:787622/69‑1 (MQ=255) aTCTGTCTGGAGCCGCAAGCCTGCCTACTGGCAGCAACGGTTGGCACCGCGGAACAGAAAGCGCAtctc < 1:170972/69‑1 (MQ=255) aTCTGTCTGGAGCCGCAAGCCTGCCTACTGGCAGCAACGGTTGGCACCGCGGAACAGAAAGCGCAtctc < 1:3384460/69‑1 (MQ=255) aTCTGTCTGGAGCCGCAAGCCTGCCTACTGGCAGCAACGGTTGGCACCGCGGAACAGAAAGCGCAtctc < 1:3380555/69‑1 (MQ=255) aTCTGTCTGGAGCCGCAAGCCTGCCTACTGGCAGCAACGGTTGGCACCGCGGAACAGAAAGCGCAtctc < 1:1791815/69‑1 (MQ=255) aTCTGTCTGGAGCCGCAAGCCTGCCTACTGGCAGCAACGGTTGGCACCGCGGAACAGAAAGCGCAtctc < 1:139144/69‑1 (MQ=255) aTCTGTCTGGAGCCGCAAGCCTGCCTACTGGCAGCAACGGTTGGCACCGCGGAACAGAAAGCGCAtctc < 1:3337246/69‑1 (MQ=255) aTCTGTCTGGAGCCGCAAGCCTGCCTACTGGCAGCAACGGTTGGCACCGCGGAACAGAAAGCGCAtctc < 1:3249153/69‑1 (MQ=255) aTCTGTCTGGAGCCGCAAGCCTGCCTACTGGCAGCAACGGTTGGCACCGCGGAACAGAAAGCGCAtctc < 1:1957864/69‑1 (MQ=255) aTCTGTATGGAGCCGCAAGCCTGCCTACTGGCAGCAACGGTTGGCACCGCGGAACAGAAAGCGCAtctc < 1:2078249/69‑1 (MQ=255) tctgtctgGAGCCGCAAGCCTGCCTACTGGCAGCAACGGTTGGCACCGCGGAACAGAAAGCGCAtctc < 1:2982365/68‑1 (MQ=255) tgtctgGAGCCGCAAGCCTGCCTACTGGCAGCAACGGTTGGCACCGCGGAACAGAAAGCGCATCTCAAt > 1:1763912/1‑69 (MQ=255) tgtctgGAGCCGCAAGCCTGCCTACTGGCAGCAACGGTTGGCACCGCGGAACAGAAAGCGCATCTCAAt > 1:1345425/1‑69 (MQ=255) tgtctgGAGCCGCAAGCCTGCCTACTGGCAGCAACGGTTGGCACCGCGGAACAGAAAGCGCATCTCAAt > 1:2907992/1‑69 (MQ=255) tgGAGCCGCAAGCCTGCCTACTGGCAGCAACGGTTGGCACCGCGGAACAGAAAgc > 1:2963653/1‑55 (MQ=255) tgGAGCCGCAAGCCTGCCTACTGGCAGCAACGGTTGGCACCGCGGAACAGAAAGCGCATCTCAATAc > 1:2889395/1‑67 (MQ=255) tgGAGCCGCAAGCCTGCCTACTGGCAGCAACGGTTGGCACCGCGGAACAGAAAGCGCATCTCAATACg > 1:2200899/1‑68 (MQ=255) tgGAGCCGCAAGCCTGCCTACTGGCAGCAACGGTTGGCACCGCGGAACAGAAAGCGCATCTCAATACg > 1:3342440/1‑68 (MQ=255) tgGAGCCGCAAGCCTGCCTACTGGCAGCAACGGTTGGCACCGCGGAACAGAAAGCGCATCTCAATACg > 1:2613205/1‑68 (MQ=255) tgGAGCCGCAAGCCTGCCTACTGGCAGCAACGGTTGGCACCGCGGAACAGAAAGCGCATCTCAATACg > 1:2148370/1‑68 (MQ=255) tgGAGCCGCAAGCCTGCCTACTGGCAGCAACGGTTGGCACCGCGGAACAGAAAGCGCATCTCAATACg > 1:900344/1‑68 (MQ=255) tgGAGCCGCAAGCCTGCCTACTGGCAGCAACGGTTGGCACCGCGGAACAGAAAGCGCATCTCAATACg > 1:2076586/1‑68 (MQ=255) tgGAGCCGCAAGCCTGCCTACTGGCAGCAACGGTTGGCACCGCGGAACAGAAAGCGCATCTCAATACg > 1:1389403/1‑68 (MQ=255) tgGAGCCGCAAGCCTGCCTACTGGCAGCAACGGTTGGCACCGCGGAACAGAAAGCGCATCTCAATACg > 1:77984/1‑68 (MQ=255) tgGAGCCGCAAGCCTGCCTACTGGCAGCAACGGTTGGCACCGCGGAACAGAAAGCGCATCTCAATACg > 1:149260/1‑68 (MQ=255) tgGAGCCGCAAGCCTGCCTACTGGCAGCAACGGTTGGCACCGCGGAACAGAAAGAGCATCTCAATACg > 1:1338836/1‑68 (MQ=255) gCCTCAAGCCTGCCTACTGGCAGCAACGGTTGGCACCGCGGAACAGAAAGCGCATCTCAATACGtt < 1:3175750/66‑1 (MQ=255) gCCGCAAGCCTGCCTACTGGCAGCAACGGTTGGCACCGCGGAACAGAAAGCGCATCTCAATACGtt < 1:2252304/66‑1 (MQ=255) gCCGCAAGCCTGCCTACTGGCAGCAACGGTTGGCACCGCGGAACAGAAAGCGCATCTCAATACGtt < 1:2583699/66‑1 (MQ=255) gCCGCAAGCCTGCCTACTGGCAGCAACGGTTGGCACCGCGGAACAGAAAGCGCATCTCAATACGtt < 1:1661507/66‑1 (MQ=255) gCCGCAAGCCTGCCTACTGGCAGCAACGGTTGGCACCGCGGAACAGAAAGCGCATCTCAATACGtt < 1:286758/66‑1 (MQ=255) gCCGCAAGCCTGCCTACTGGCAGCAACGGTTGGCACCGCGGAACAGAAAGCGCATCTCAATACGtt < 1:2919798/66‑1 (MQ=255) gCCGCAAGCCTGCCTACTGGCAGCAACGGTTGGCACCGCGGAACAGAAAGCGCATCTCAATACGtt < 1:1097783/66‑1 (MQ=255) aGCCTGCCTACTGGCAGCAACGGTTGGCACCGCGGAACAGAAAGCGCATCTCAATACGTTAATGGCCGa < 1:1421243/69‑1 (MQ=255) aGCCTGCCTACTGGCAGCAACGGTTGGCACCGCGGAACAGAAAGCGCATCTCAATACGTTAATGGCCGa < 1:2758355/69‑1 (MQ=255) aGCCTGCCTACTGGCAGCAACGGTTGGCACCGCGGAACAGAAAGCGCATCTCAATACGTTAATGGCCGa < 1:3282976/69‑1 (MQ=255) aGCCTGCCTACTGGCAGCAACGGTTGGCACCGCGGAACAGAAAGCGCATCTCAATACGTTAATGGCCGa < 1:895560/69‑1 (MQ=255) aGCCTGCCTACTGGCAGCAACGGTTGGCACCGCGGAACAGAAAGCGCATCTCAATACGTTAATGGCCGa < 1:1445497/69‑1 (MQ=255) aGCCTGCCTACTGGCAGCAACGGTTGGCACCGCGGAACAGAAAGCGCATCTCAATACGTTAATGGCCGa < 1:38817/69‑1 (MQ=255) aGCCTGCCTACTGGCAGCAACGGTTGGCACCGCGGAACAGAAAGCGCATCTCAATACGTTAATGGCCGa < 1:43500/69‑1 (MQ=255) gcctgcctACTGGCAGCAACGGTTGGCACCGCGGAACAGAAAGCGCATCTCAATACGTTAACGGCCGa < 1:2744777/68‑1 (MQ=255) aCGGTTGGCACCGCGGAACAGAAAGCGCATCTCAATACGTTAATGGCCGAAATGGCGGCATTAAAagag > 1:2118659/1‑69 (MQ=255) aggTTGGCACCGCGGAACAGAAGGCGCATCTCAATACGTTa > 1:283747/2‑41 (MQ=39) aggTTGGCACCGCGGAACAGAAGGCGCATCTCAATACGTTa > 1:2103333/2‑41 (MQ=39) aggTTGGCACCGCGGAACAGAAGGCGCATCTCAATACGTTa > 1:301868/2‑41 (MQ=39) aggTTGGCACCGCGGAACAGAAGGCGCATCTCAATACGTTa > 1:1111142/2‑41 (MQ=39) aggTTGGCACCGCGGAACAGAAGGCGCATCTCAATACGTTa > 1:817373/2‑41 (MQ=39) aggTTGGCACCGCGGAACAGAAGGCGCATCTCAATACGTTa > 1:2890796/2‑41 (MQ=39) aggTTGGCACCGCGGAACAGAAGGCGCATCTCAATACGTTa > 1:1257145/2‑41 (MQ=39) aggTTGGCACCGCGGAACAGAAGGCGCATCTCAATACGTTa > 1:1333969/2‑41 (MQ=39) aggTTGGCACCGCGGAACAGAAGGCGCATCTCAATACGTTa > 1:2511258/2‑41 (MQ=39) aggTTGGCACCGCGGAACAGAAGGCGCATCTCAATACGTTa > 1:2258342/2‑41 (MQ=39) aggTTGGCACCGCGGAACAGAAGGCGCATCTCAATACGTTa > 1:2776016/2‑41 (MQ=39) aggTTGGCACCGCGGAACAGAAGGCGCATCTCAATACGTTa > 1:2683240/2‑41 (MQ=39) aggTTGGCACCGCGGAACAGAAGGCGCATCTCAATACGTTa > 1:2415535/2‑41 (MQ=39) aggTTGGCACCGCGGAACAGAAGGCGCATCTCAATACGTTa > 1:4581/2‑41 (MQ=39) aggTTGGCACCGCGGAACAGAAGGCGCATCTCAATACGTTa > 1:245750/2‑41 (MQ=39) ttcgCACCGCGGAACAGAAGCCGCATCTCAATACGTTAATGGCCGAAATGGCGGCATTAAAAGAGAACt < 1:499557/66‑1 (MQ=255) ttGGCACCGCGGAACAGAAGGCGCATCTCAATACGTTAATGGCCGAAATGGCGGCATTAAAAGAGAACt < 1:3388910/69‑1 (MQ=255) ttGGCACCGCGGAACAGAAGGCGCATCTCAATACGTTAATGGCCGAAATGGCGGCATTAAAAGAGAACt < 1:257202/69‑1 (MQ=255) ttGGCACCGCGGAACAGAAGGCGCATCTCAATACGTTAATGGCCGAAATGGCGGCATTAAAAGAGAACt < 1:3338297/69‑1 (MQ=255) ttGGCACCGCGGAACAGAAGGCGCATCTCAATACGTTAATGGCCGAAATGGCGGCATTAAAAGAGAACt < 1:2212215/69‑1 (MQ=255) ttGGCACCGCGGAACAGAAGGCGCATCTCAATACGTTAATGGCCGAAATGGCGGCATTAAAAGAGAACt < 1:1076188/69‑1 (MQ=255) ttGGCACCGCGGAACAGAAGGCGCATCTCAATACGTTAATGGCCGAAATGGCGGCATTAAAAGAGAACt < 1:1453825/69‑1 (MQ=255) ttGGCACCGCGGAACAGAAGGCGCATCTCAATACGTTAATGGCCGAAATGGCGGCATTAAAAGAGAACt < 1:1748181/69‑1 (MQ=255) ttGGCACCGCGGAACAGAAGGCGCATCTCAATACGTTAATGGCCGAAATGGCGGCATTAAAAGAGAACt < 1:2345842/69‑1 (MQ=255) ttGGCACCGCGGAACAGAAGGCGCATCTCAATACGTTAATGGCCGAAATGGCGGCATTAAAAGAGAACt < 1:2511981/69‑1 (MQ=255) ttGGCACCGCGGAACAGAAGGCGCATCTCAATACGTTAATGGCCGAAATGGCGGCATTAAAAGAGAACt < 1:262299/69‑1 (MQ=255) ttGGCACCGCGGAACAGAAGGCGCATCTCAATACGTTAATGGCCGAAATGGCGGCATTAAAAGAGAACt < 1:2859764/69‑1 (MQ=255) cgGAACAGAAAGCGCATCTCAATACGTTAATGGCCGAAATGGCGGCATTAAAAGAGAACTTTCGCCGTg < 1:2014630/69‑1 (MQ=255) cgGAACAGAAAGCGCATCTCAATACGTTAATGGCCGAAATGGCGGCATTAAAAGAGAACTTTCGCCGTg < 1:1941412/69‑1 (MQ=255) cgGAACAGAAAGCGCATCTCAATACGTTAATGGCCGAAATGGCGGCATTAAAAGAGAACTTTCGCCGTg < 1:2288181/69‑1 (MQ=255) cAGAAAGCGCATCTCAATACGTTAATGGCCGAAATGGCGGCATTAAAAGAGAACTTTCGCCGTGACCg > 1:3103625/1‑68 (MQ=255) cAGAAAGCGCATCTCAATACGTTAATGGCCGAAATGGCGGCATTAAAAGAGAACTTTCGCCGTGAACGc > 1:3019740/1‑69 (MQ=255) cAGAAAGCGCATCTCAATACGTTAATGGCCGAAATGGCGGCATTAAAAGAGAACTTTCGCCGTGAACGc > 1:347126/1‑69 (MQ=255) cAGAAAGCGCATCTCAATACGTTAATGGCCGAAATGGCGGCATTAAAAGAGAACTTTCGCCGTGAACGc > 1:1148279/1‑69 (MQ=255) cAGAAAGCGCATCTCAATACGTTAATGGCCGAAATGGCGGCATTAAAAGAGAACTTTCGCCGTGAACGc > 1:437392/1‑69 (MQ=255) cAGAAAGCGCATCTCAATACGTTAATGGCCGAAATGGCGGCATTAAAAGAGAACTTTCGCCGTGAACGc > 1:2099611/1‑69 (MQ=255) cAGAAAGCGCATCTCAATACGTTAATGGCCGAAATGGCGGCATTAAAAGAGAACTTTCGCCGTGAACGc > 1:1005473/1‑69 (MQ=255) cAGAAAGCGCATCTCAATACGTTAATGGCCGAAATGGCGGCATTAAAAGAGAACTTTCGCCGTGAACGc > 1:2358178/1‑69 (MQ=255) cAGAAAGCGCATCTCAATACGTTAATGGCCGAAATGGCGGCATTAAAAGAGAACTTTCGCCGTGAACGc > 1:766209/1‑69 (MQ=255) cAGAAAGCGCATCTCAATACGTTAATGGCCGAAATGGCGGCATTAAAAGAGAACTTTCGCCGTGAACGc > 1:3306524/1‑69 (MQ=255) cAGAAAGCGCATCTCAATACGTTAATGGCCGAAATGGCGGCATTAAAAGAGAACTTTCGCCGTGAACGc > 1:1505024/1‑69 (MQ=255) cAGAAAGCGCATCTCAATACGTTAATGGCCGAAATGGCGGAATTAAAAGAGAACTTTCGCCGTGAACGc > 1:679802/1‑69 (MQ=255) aaGCGCATCTCAATACGTTAATGGCCGAAATGGCGGCATTAAAAgaga < 1:2738213/48‑1 (MQ=255) aaGCGCATCTCAATACGTTAATGGCCGAAATGGCGGCATTAAAAgaga < 1:1783430/48‑1 (MQ=255) aaGCGCATCTCAATACGTTAATGGCCGAAATGGCGGCATTAAAAgaga < 1:589718/48‑1 (MQ=255) aaGCGCATCTCAATACGTTAATGGCCGAAATGGCGGCATTAAAAgaga < 1:2269705/48‑1 (MQ=255) aaGCGCATCTCAATACGTTAATGGCCGAAATGGCGGCATTAAAAgaga < 1:3405362/48‑1 (MQ=255) | ATGCGTATCTGTCTGGAGCCGCAAGCCTGCCTACTGGCAGCAACGGTTGGCACCGCGGAACAGAAAGCGCATCTCAATACGTTAATGGCCGAAATGGCGGCATTAAAAGAGAACTTTCGCCGTGAACGC > minE/2457616‑2457744 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |