| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 208,575 | C→T | 33.8% | I195I (ATC→ATT) | dkgB → | 2,5‑diketo‑D‑gluconate reductase B |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 208,575 | 0 | C | T | 33.8% | 101.2 / 67.6 | 124 | I195I (ATC→ATT) | dkgB | 2,5‑diketo‑D‑gluconate reductase B |
| Reads supporting (aligned to +/- strand): ref base C (46/36); new base T (12/30); total (58/66) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.38e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.38e-01 | |||||||||||
Only 100 of 124 total aligned reads displayed.
CATATTACTTCCTATATGACGCTGGCGTATGGTAAGGCCCTGAAAGATGAGGTTATTGCTCGTATCGCAGCTAAACACAATGCGACTCCGGCACAAGTGATTCTGGCGTGGGCTATGGGGGA > minE/208510‑208631 | cATATTACTTCCTATATGACGCTGGCGTATGGTAAGGCCCTGAAAGATGAGGTTATTGCTCGTATCGCa > 1:1003909/1‑69 (MQ=255)cATATTACTTCCTATATGACGCTGGCGTATGGTAAGGCCCTGAAAGATGAGGTTATTGCTCGTATCGCa > 1:12262/1‑69 (MQ=255) ttACTTCCTATATGACGCTGGCGTATGGTAAGGCCCTGAAAGATGAGGTTATTGCTCGTATCGCAGCt > 1:1326627/1‑68 (MQ=255) ttACTTCCTATATGACGCTGGCGTATGGTAAGGCCCTGAAAGATGAGGTTATTGCTCGTATCGCAGCTa > 1:1389836/1‑69 (MQ=255) ttACTTCCTATATGACGCTGGCGTATGGTAAGGCCCTGAAAGATGAGGTTATTGCTCGTATCGCAGCTa > 1:2268360/1‑69 (MQ=255) ttACTTCCTATATGACGCTGGCGTATGGTAAGGCCCTGAAAGATGAGGTTATTGCTCGTATCGCAGCTa > 1:1498611/1‑69 (MQ=255) ttACTTCCTATATGACGCTGGCGTATGGTAAGGCCCTGAAAGATGAGGTTATTGCTCGTATCGCAGCTa > 1:705270/1‑69 (MQ=255) ttACTTCCTATATGACGCTGGCGTATGGTAAGGCCCTGAAAGATGAGGTTATTGCTCGTATCGCAGCTa > 1:2101360/1‑69 (MQ=255) ttACTTCCTATATGACGCTGGCGTATGGTAAGGCCCTGAAAGATGAGGTTATTGCTCGTATCGCAGCTa > 1:3216787/1‑69 (MQ=255) ttACTTCCTATATGACGCTGGCGTATGGTAAGGCCCTGAAAGATGAGGTTATTGCTCGTATCGCAGCTa > 1:2060617/1‑69 (MQ=255) ttACTTCCTATATGACGCTGGCGTATGGTAAGGCCCTGAAAGATGAGGTTATTGCTCGTATCGCAGCTa > 1:2676355/1‑69 (MQ=255) ttACTTCCTATATGACGCTGGCGTATGGTAAGGCCCTGAAAGATGAGGTTATTGCTCGTATCGCAGCTa > 1:1948819/1‑69 (MQ=255) ttACTTCCTATATGACGCTGGCGTATGGTAAGGCCCTGAAAGATGAGGTTATTGCTCGTATCGCAGCTa > 1:2611728/1‑69 (MQ=255) ttACTTCCTATATGACGCTGGCGTATGGTAAGGCCCTGAAAGATGAGGTTATTGCTCGTATCGCAGCTa > 1:1968145/1‑69 (MQ=255) ttACTTCCTATATGACGCTGGCGTATGGTAAGGCCCTGAAAGATGAGGTTATTGCTCGTATCGCAGCTa > 1:250276/1‑69 (MQ=255) aCTTCCTATATGACGCTGGCGTATGGTAAGGCCCTGAAAGATGAGGTTATTGCTCGTATCGCAGCTaaa < 1:3131083/69‑1 (MQ=255) aCTTCCTATATGACGCTGGCGTATGGTAAGGCCCTGAAAGATGAGGTTATTGCTCGTATCGCAGCTaaa < 1:2150722/69‑1 (MQ=255) aCTTCCTATATGACGCTGGCGTATGGTAAGGCCCTGAAAGATGAGGTTATTGCTCGTATCGCAGCTaaa < 1:3117586/69‑1 (MQ=255) aCTTCCTATATGACGCTGGCGTATGGTAAGGCCCTGAAAGATGAGGTTATTGCTCGTATCGCAGCTaaa < 1:1032590/69‑1 (MQ=255) aCTTCCTATATGACGCTGGCGTATGGTAAGGCCCTGAAAGATGAGGTTATTGCTCGTATCGCAGCTaaa < 1:2822245/69‑1 (MQ=255) aCTTCCTATATGACGCTGGCGTATGGTAAGGCCCTGAAAGATGAGGTTATTGCTCGTATCGCAGCTaaa < 1:681717/69‑1 (MQ=255) aCTTCCTATATGACGCTGGCGTATGGTAAGGCCCTGAAAGATGAGGTTATTGCTCGTATCGCAGCTaaa < 1:2444844/69‑1 (MQ=255) aCTTCCTATATGACGCTGGCGTATGGTAAGGCCCTGAAAGATGAGGTTATTGCTCGTATCGCAGCTaaa < 1:1088427/69‑1 (MQ=255) cTTCCTATATGACGCTGGCGTATGGTAAGGCCCTGAAAGATGAGGTTATTGCTCGTATCGCAGCTaaa < 1:627091/68‑1 (MQ=255) cTTCCTATATGACGCTGGCGTATGGTAAGGCCCTGAAAGATGAGGTTATTGCTCGTATCGCAGCTaaa < 1:1488852/68‑1 (MQ=255) cTTCCTATATGACGCTGGCGTATGGTAAGGCCCTGAAAGATGAGGTTATTGCTCGTATCGCAGCTaaa < 1:2968192/68‑1 (MQ=255) tCCTATATGACTCTGGCGTATGGCAATGCACTGAAAGATAAGGTTATTGCTCGTATTGCAGCTAAaca < 1:2940235/68‑1 (MQ=255) tCCTATATGACTCTGGCGTATGGCAATGCACTGAAAGATAAGGTTATTGCTCGTATTGCAGCTAAaca < 1:2023031/68‑1 (MQ=255) tCCTATATGACTCTGGCGTATGGCAATGCACTGAAAGATAAGGTTATTGCTCGTATTGCAGCTAAaca < 1:1814549/68‑1 (MQ=255) tCCTATATGACTCTGGCGTATGGCAATGCACTGAAAGATAAGGTTATTGCTCGTATTGCAGCTAAaca < 1:1787404/68‑1 (MQ=255) tCCTATATGACTCTGGCGTATGGCAATGCACTGAAAGATAAGGTTATTGCTCGTATTGCAGCTAAaca < 1:1737558/68‑1 (MQ=255) tCCTATATGACTCTGGCGTATGGCAATGCACTGAAAGATAAGGTTATTGCTCGTATTGCAGCTAAaca < 1:1088885/68‑1 (MQ=255) tCCTATATGACTCTGGCGTATGGCAATGCACTGAAAGATAAGGTTATTGCTCGTATTGCAGCTAAaca < 1:2362900/68‑1 (MQ=255) tCCTATATGACTCTGGCGTATGGCAATGCACTGAAAGATAAGGTTATTGCTCGTATTGCAGCTAAaca < 1:3055588/68‑1 (MQ=255) tCCTATATGACTCTGGCGTATGGCAATGCACTGAAAGATAAGGTTATTGCTCGTATTGCAGCTAAaca < 1:3368111/68‑1 (MQ=255) tCCTATATGACTCTGGCGTATGGCAATGCACTGAAAGATAAGGTTATTGCTCGTATTGCAGCTAAaca < 1:1165364/68‑1 (MQ=255) tCCTATATGACTCTGGCGTATGGCAATGCACTGAAAGATAAGGTTATTGCTCGTATTGCAGCTAAaca < 1:1402167/68‑1 (MQ=255) tatGACGCTGGCGTATGGTAAGGCCCTGAAAGATGAGGTTATTGCTCGTATCGCAGCTACACACAATGc > 1:17073/1‑69 (MQ=255) tatGACGCTGGCGTATGGTAAGGCCCTGAAAGATGAGGTTATTGCTCGTATCGCAGCTAAacac < 1:2597360/64‑1 (MQ=255) tatGACGCTGGCGTATGGTAAGGCCCTGAAAGATGAGGTTATTGCTCGTATCGCAGCTAAACACAATg > 1:2415118/1‑68 (MQ=255) tatGACGCTGGCGTATGGTAAGGCCCTGAAAGATGAGGTTATTGCTCGTATCGCAGCTAAACACAATGc > 1:1663353/1‑69 (MQ=255) tatGACGCTGGCGTATGGTAAGGCCCTGAAAGATGAGGTTATTGCTCGTATCGCAGCTAAACACAATGc > 1:2606984/1‑69 (MQ=255) tatGACGCTGGCGTATGGTAAGGCCCTGAAAGATGAGGTTATTGCTCGTATCGCAGCTAAACACAATGc > 1:552153/1‑69 (MQ=255) tatGACGCTGGCGTATGGTAAGGCCCTGAAAGATGAGGTTATTGCTCGTATCGCAGCTAAACACAATGc > 1:1375014/1‑69 (MQ=255) tGACGCTGGCGTATGGTAAGGCCCTGAAAGATGAGGTTATTGCTCGTATCGCAGCTaaa < 1:630564/59‑1 (MQ=255) gTATGGTAAGGCCCTGAAAGATGAGGTTATTGCTCGTATCGCAGCTAAACACAATGCGACTCCGGcaca < 1:1441760/69‑1 (MQ=255) gTATGGTAAGGCCCTGAAAGATGAGGTTATTGCTCGTATCGCAGCTAAACACAATGCGACTCCGGcaca < 1:3212439/69‑1 (MQ=255) gTATGGTAAGGCCCTGAAAGATGAGGTTATTGCTCGTATCGCAGCTAAACACAATGCGACTCCGGcaca < 1:1082987/69‑1 (MQ=255) aGGCCCTGAAAGATGAGGTTATTGCTCGTATCGCAGCTAAACACAATGCGACTCCGGCACAAGTGATTc > 1:1674433/1‑69 (MQ=255) aGGCCCTGAAAGATGAGGTTATTGCTCGTATCGCAGCTAAACACAATGCGACTCCGGCACAAGTGATTc > 1:2383945/1‑69 (MQ=255) cTGAAAGATGAGGTTATTGCTCGTATCGCAGCTAAACACAATGCGACTCCGGCACAAGTGATTCTGGCg > 1:2706009/1‑69 (MQ=255) cTGAAAGATAAGGTTATTGCTCGTATTGCAGCTAAACACAATGCGACTCCGGCTCAAGTGATTCTGGCg < 1:2300912/69‑1 (MQ=255) cTGAAAGATAAGGTTATTGCTCGTATTGCAGCTAAACACAATGCGACTCCGGCTCAAGTGATTCTGGCg < 1:1027493/69‑1 (MQ=255) cTGAAAGATAAGGTTATTGCTCGTATTGCAGCTAAACACAATGCGACTCCGGCTCAAGTGATTCTGGCg < 1:91375/69‑1 (MQ=255) cTGAAAGATAAGGTTATTGCTCGTATTGCAGCTAAACACAATGCGACTCCGGCTCAAGTGATTCTGGCg < 1:1080711/69‑1 (MQ=255) cTGAAAGATAAGGTTATTGCTCGTATTGCAGCTAAACACAATGCGACTCCGGCTCAAGTGATTCTGGCg < 1:876586/69‑1 (MQ=255) cTGAAAGATAAGGTTATTGCTCGTATTGCAGCTAAACACAATGCGACTCCGGCTCAAGTGATTCTGGCg < 1:34977/69‑1 (MQ=255) aGATGAGGTTATTGCTCGTATCGCAGCTAAACACAATGCGACTCCGGCACAAGTGATTCTGGCGTgg < 1:297059/67‑1 (MQ=255) aGATGAGGTTATTGCTCGTATCGCAGCTAAACACAATGCGACTCCGGCACAAGTGATTCTGGCGTgg < 1:962383/67‑1 (MQ=255) aGATGAGGTTATTGCTCGTATCGCAGCTAAACACAATGCGACTCCGGCACAAGTGATTCTGGCGTgg < 1:1570975/67‑1 (MQ=255) aGATGAGGTTATTGCTCGTATCGCAGCTAAACACAATGCGACTCCGGCACAAGTGATTCTGGCGTgg < 1:2223739/67‑1 (MQ=255) ttaGGTTATTGCTCGTATCGCAGCTAAACACAATGCGACTCCGGCACAAGTGATTCTGGCGTGGGCTAt < 1:2482348/67‑1 (MQ=255) tGAGGTTATTGCTCGTATCGCAGCTAAACACAATGCGACTCCGGCACAAGTGATTCTGGCGTGGGCTAt < 1:916283/69‑1 (MQ=255) tGAGGTTATTGCTCGTATCGCAGCTAAACACAATGCGACTCCGGCACAAGTGATTCTGGCGTGGGCTAt < 1:167051/69‑1 (MQ=255) tGAGGTTATTGCTCGTATCGCAGCTAAACACAATGCGACTCCGGCACAAGTGATTCTGGCGTGGGCTAt < 1:2538913/69‑1 (MQ=255) tGAGGTTATTGCTCGTATCGCAGCTAAACACAATGCGACTCCGGCACAAGTGATTCTGGCGTGGGCTAt < 1:1987090/69‑1 (MQ=255) tGAGGTTATTGCTCGTATCGCAGCTAAACACAATGCGACTCCGGCACAAGTGATTCTGGCGTGGGCTAt < 1:2742195/69‑1 (MQ=255) aGGTTATTGCTCGTATCGCAGCTAAACACAATGCGACTCCGGCACAAGTGATTCTGGCGTGGGCTATgg > 1:223263/1‑69 (MQ=255) aGGTTATTGCTCGTATCGCAGCTAAACACAATGCGACTCCGGCACAAGTGATTCTGGCGTGGGCTATgg > 1:430610/1‑69 (MQ=255) aGGTTATTGCTCGTATCGCAGCTAAACACAATGCGACTCCGGCACAAGTGATTCTGGCGTGGGCTATgg > 1:1359603/1‑69 (MQ=255) aGGTTATTGCTCGTATCGCAGCTAAACACAATGCGACTCCGGCACAAGTGATTCTGGCGTGGGCTATgg > 1:640211/1‑69 (MQ=255) aGGTTATTGCTCGTATCGCAGCTAAACACAATGCGACTCCGGCACAAGTGATTCTGGCGTGGGCTATgg > 1:2820492/1‑69 (MQ=255) aGGTTATTGCTCGTATCGCAGCTAAACACAATGCGACTCCGGCACAAGTGATTCTGGCGTGGGCTATgg > 1:739788/1‑69 (MQ=255) aGGTTATTGCTCGTATCGCAGCTAAACACAATGCGACTCCGGCACAAGTGATTCTGGCGTGGGCTATgg > 1:781231/1‑69 (MQ=255) aGGTTATTGCTCGTATCGCAGCTAAACACAATGCGACTCCGGCACAAGTGATTCTGGCGTGGGCTATgg > 1:785242/1‑69 (MQ=255) aGGTTATTGCTCGTATCGCAGCTAAACACAATGCGACTCCGGCACAAGTGATTCTGGCGTGGGCTATgg > 1:2624774/1‑69 (MQ=255) aGGTTATTGCTCGTATCGCAGCTAAACACAATGCGACTCCGGCACAAGTGATTCTGGCGTGGGCTATgg > 1:1005314/1‑69 (MQ=255) tATTGCTCGTATTGCAGCTAAACACAATGCGACTCCGGCTCAAGTGATTCTGGCGTGGGCTATGGGGGa > 1:1701040/1‑69 (MQ=255) tATTGCTCGTATTGCAGCTAAACACAATGCGACTCCGGCTCAAGTGATTCTGGCGTGGGCTATGGGGGa > 1:1185317/1‑69 (MQ=255) tATTGCTCGTATTGCAGCTAAACACAATGCGACTCCGGCTCAAGTGATTCTGGCGTGGGCTATGGGGGa > 1:1449512/1‑69 (MQ=255) tATTGCTCGTATTGCAGCTAAACACAATGCGACTCCGGCTCAAGTGATTCTGGCGTGGGCTATGGGGGa > 1:568438/1‑69 (MQ=255) tATTGCTCGTATTGCAGCTAAACACAATGCGACTCCGGCTCAAGTGATTCTGGCGTGGGCTATGGGGGa > 1:479746/1‑69 (MQ=255) tATTGCTCGTATTGCAGCTAAACACAATGCGACTCCGGCTCAAGTGATTCTGGCGTGGGCTATGGGGGa > 1:1129860/1‑69 (MQ=255) tATTGCTCGTATTGCAGCTAAACACAATGCGACTCCGGCTCAAGTGATTCTGGCGTGGGCTATGGGGGa > 1:1815465/1‑69 (MQ=255) tATTGCTCGTATTGCAGCTAAACACAATGCGACTCCGGCTCAAGTGATTCTGGCGTGGGCTATGGGGGa > 1:2090795/1‑69 (MQ=255) tATTGCTCGTATTGCAGCTAAACACAATGCGACTCCGGCTCAAGTGATTCTGGCGTGGGCTATGGGGGa > 1:3023188/1‑69 (MQ=255) tATTGCTCGTATTGCAGCTAAACACAATGCGACTCCGGCTCAAGTGATTCTGGCGTGGGCTATGGGGGa > 1:2642548/1‑69 (MQ=255) tATTGCTCGTATTGCAGCTAAACACAATGCGACTCCGGCACAAGTGATTCTGGCGTGGGCTATGGGGGa > 1:2394173/1‑69 (MQ=255) aTTGCTCGTATTGCAGCTAAACACAATGCGACTCCGGCACAAGTGATTCTGGCGTGGGc < 1:3371596/59‑1 (MQ=255) aTTGCTCGTATTGCAGCTAAACACAATGCGACTCCGGCACAAGTGATTCTGGCGTGGGc < 1:1946676/59‑1 (MQ=255) aTTGCTCGTATTGCAGCTAAACACAATGCGACTCCGGCACAAGTGATTCTGGCGTGGGc < 1:2208407/59‑1 (MQ=255) aTTGCTCGTATTGCAGCTAAACACAATGCGACTCCGGCACAAGTGATTCTGGCGTGGGc < 1:2288097/59‑1 (MQ=255) aTTGCTCGTATTGCAGCTAAACACAATGCGACTCCGGCACAAGTGATTCTGGCGTGGGc < 1:2756094/59‑1 (MQ=255) aTTGCTCGTATTGCAGCTAAACACAATGCGACTCCGGCACAAGTGATTCTGGCGTGGGc < 1:2436012/59‑1 (MQ=255) aTTGCTCGTATTGCAGCTAAACACAATGCGACTCCGGCACAAGTGATTCTGGCGTGGGc < 1:2667459/59‑1 (MQ=255) aTTGCTCGTATCGCAGCTAAACACAATGCGACTCCGGCACAAGTGATTCTGGCGTGGGc < 1:25109/59‑1 (MQ=255) aTTGCTCGTATCGCAGCTAAACACAATGCGACTCCGGCACAAGTGATTCTGGCGTGGGc < 1:357582/59‑1 (MQ=255) aTTGCTCGTATCGCAGCTAAACACAATGCGACTCCGGCACAAGTGATTCTGGCGTGGGc > 1:1036083/1‑59 (MQ=255) aTTGCTCGTATCGCAGCTAAACACAATGCGACTCCGGCACAAGTGATTCTGGCGTGGGc > 1:1348831/1‑59 (MQ=255) aTTGCTCGTATCGCAGCTAAACACAATGCGACTCCGGCACAAGTGATTCTGGCGTGGGc > 1:1537295/1‑59 (MQ=255) | CATATTACTTCCTATATGACGCTGGCGTATGGTAAGGCCCTGAAAGATGAGGTTATTGCTCGTATCGCAGCTAAACACAATGCGACTCCGGCACAAGTGATTCTGGCGTGGGCTATGGGGGA > minE/208510‑208631 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |