| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,459,646 | C→T | 15.8% | L86L (CTG→TTG) | asnC → | DNA‑binding transcriptional dual regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,459,646 | 0 | C | T | 15.8% | 82.9 / 5.3 | 50 | L86L (CTG→TTG) | asnC | DNA‑binding transcriptional dual regulator |
| Reads supporting (aligned to +/- strand): ref base C (36/6); new base T (6/2); total (42/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.98e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TCGGTTATGACGTAGGCTGCTTTATCGGCATTATATTAAAGAGCGCCAAAGACTACCCTTCCGCGCTGGCAAAGCTGGAAAGCCTTGATGAAGTCACTGAAGCCTATTACACAACCGGCCACTACAGCATCTT > minE/2459581‑2459713 | tCGGTTATGACGTAGGCTGCTTTATCGGCATTATATTAAAGAGCGCCAAAGACTACCCTTCCGCGCTg > 1:3304488/1‑68 (MQ=255)tCGGTTATGACGTAGGCTGCTTTATCGGCATTATATTAAAGAGCGCCAAAGACTACCCTTCCGCGCTg > 1:1905122/1‑68 (MQ=255) aCGTAGGCTGCTTTATCGGCATTATATTAAAGAGCGCCAAAGACTACCCTTCCGCGCTGGCAAAGCTg > 1:1181569/1‑68 (MQ=255) aCGTAGGCTGCTTTATCGGCATTATATTAAAGAGCGCCAAAGACTACCCTTCCGCGCTGGCAAAGCTg > 1:60380/1‑68 (MQ=255) aCGTAGGCTGCTTTATCGGCATTATATTAAAGAGCGCCAAAGACTACCCTTCCGCGCTGGCAAAGCTg > 1:629883/1‑68 (MQ=255) aCGTAGGCTGCTTTATCGGCATTATATTAAAGAGCGCCAAAGACTACCCTTCCGCGCTGGCAAAGCTg > 1:706839/1‑68 (MQ=255) aCGTAGGCTGCTTTATCGGCATTATATTAAAGAGCGCCAAAGACTACCCTTCCGCGCTGGCAAAGCTg > 1:2497020/1‑68 (MQ=255) aCGTAGGCTGCTTTATCGGCATTATATTAAAGAGCGCCAAAGACTACCCTTCCGCGCTGGCAAAGCTg > 1:2229608/1‑68 (MQ=255) aCGTAGGCTGCTTTATCGGCATTATATTAAAGAGCGCCAAAGACTACCCTTCCGCGCTGGCAAAGCTg > 1:2076100/1‑68 (MQ=255) aCGTAGGCTGCTTTATCGGCATTATATTAAAGAGCGCCAAAGACTACCCTTCCGCGCTGGCAAAGCTg > 1:2027073/1‑68 (MQ=255) tATCGGTATTATATTAAAGAGCGCCAAAGACTACCCTTCCGCGTTGGCAAAGCTGGAAAGCCTtgatga > 1:198725/1‑69 (MQ=255) tATCGGTATTATATTAAAGAGCGCCAAAGACTACCCTTCCGCGTTGGCAAAGCTGGAAAGCCTtgatga > 1:1997397/1‑69 (MQ=255) tATCGGTATTATATTAAAGAGCGCCAAAGACTACCCTTCCGCGTTGGCAAAGCTGGAAAGCCTtgatga > 1:1854762/1‑69 (MQ=255) tATCGGTATTATATTAAAGAGCGCCAAAGACTACCCTTCCGCGTTGGCAAAGCTGGAAAGCCTtgatga > 1:1981380/1‑69 (MQ=255) tATCGGTATTATATTAAAGAGCGCCAAAGACTACCCTTCCGCGTTGGCAAAGCTGGAAAGCCTtgatga > 1:2401870/1‑69 (MQ=255) tATCGGTATTATATTAAAGAGCGCCAAAGACTACCCTTCCGCGTTGGCAAAGCTGGAAAGCCTtgatga > 1:2337637/1‑69 (MQ=255) atTAAAGAGCGCCAAAGACTACCCTTCCGCGCTGGCaaa > 1:2871254/1‑39 (MQ=255) atTAAAGAGCGCCAAAGACTACCCTTCCGCGCTGGCaaa > 1:1934342/1‑39 (MQ=255) atTAAAGAGCGCCAAAGACTACCCTTCCGCGCTGGCaaa > 1:372098/1‑39 (MQ=255) atTAAAGAGCGCCAAAGACTACCCTTCCGCGCTGGCaaa > 1:3224996/1‑39 (MQ=255) ttAAAGAGCGCCAAAGACTACCCTTCCGCGTTGGCAAAGCTGGAAAGCCTTGATGAAGTCACTGAAGcc < 1:8280/69‑1 (MQ=255) ttAAAGAGCGCCAAAGACTACCCTTCCGCGCTGGCAAAGCTGGAAAGCCTTGATGAAGTCACTGAAGcc > 1:759237/1‑69 (MQ=255) tAAAGAGCGCCAAAGACTACCCTTCCGCGCTGGCAAAGCTGGAAAGCCTTGATGAAGTCACTGAAGcc < 1:448810/68‑1 (MQ=255) aaaGAGCGCCAAAGACTACCCTTCCGCGTTGGCAAAGCTGGAAAGCCTTGATGAAGTCACTGAAGcc < 1:148239/67‑1 (MQ=255) aaaGAGCGCCAAAGACTACCCTTCCGCGCTGGCAAAGCTGGAAAGCCTTGATGAAGTCACTGAAGCCt < 1:3191984/68‑1 (MQ=255) aaGAGCGCCAAAGACTACCCTTCCGCGCTGGCAAAGCTGGAAAGCCTTGATGAAGTCACTGAAGcc < 1:1701627/66‑1 (MQ=255) aGACTATCCTTCCGCGCTGGCAAAGCTGGAAAGCCTcgatga > 1:664994/1‑42 (MQ=38) aGACTATCCTTCCGCGCTGGCAAAGCTGGAAAGCCTCGATGAAGTCACTGAAGCCTAtt > 1:2636683/1‑59 (MQ=255) aGACTATCCTTCCGCGCTGGCAAAGCTGGAAAGCCTCGATGAAGTCACTGAAGCCTAtt > 1:330796/1‑59 (MQ=255) aGACTATCCTTCCGCGCTGGCAAAGCTGGAAAGCCTCGATGAAGTCACTGAAGCCTAtt > 1:3352426/1‑59 (MQ=255) aGACTATCCTTCCGCGCTGGCAAAGCTGGAAAGCCTCGATGAAGTCACTGAAGCCTAtt > 1:3103187/1‑59 (MQ=255) aGACTATCCTTCCGCGCTGGCAAAGCTGGAAAGCCTCGATGAAGTCACTGAAGCCTAtt > 1:1584758/1‑59 (MQ=255) aGACTATCCTTCCGCGCTGGCAAAGCTGGAAAGCCTCGATGAAGTCACTGAAGCCTAtt > 1:23140/1‑59 (MQ=255) aGACTATCCTTCCGCGCTGGCAAAGCTGGAAAGCCTCGATGAAGTCACTGAAGCCTAtt > 1:76421/1‑59 (MQ=255) aGACTATCCTTCCGCGCTGGCAAAGCTGGAAAGCCTCGATGAAGTCACTGAAGCCTAtt > 1:783889/1‑59 (MQ=255) aGACTATCCTTCCGCGCTGGCAAAGCTGGAAAGCCTCGATGAAGTCACTGAAGCCTAtt > 1:2046835/1‑59 (MQ=255) gACTACCCTTCCGCGCTGGCAAAGCTGGAAAGCCTTGATGAAGTCACTGAAGCCTATTACACAACCGGc < 1:3184726/69‑1 (MQ=255) gACTACCCTTCCGCGCTGGCAAAGCTGGAAAGCCTTGATGAAGTCACTGAAGCCTATTACACAACCGGc < 1:2966850/69‑1 (MQ=255) gACTACCCTTCCGCGCTGGCAAAGCTGGAAAGCCTTGATGAAGTCACTGAAGCCTATTACACAACCGGc < 1:1211219/69‑1 (MQ=255) tACCCTTCCGCGCTGGCAAAGCTGGAAAGCCTTGATGAAGTCACTGAAGCCTATTa > 1:1996582/1‑56 (MQ=255) tACCCTTCCGCGCTGGCAAAGCTGGAAAGCCTTGATGAAGTCACTGAAGCCTATTACACAACCGGCCa > 1:12087/1‑68 (MQ=255) tACCCTTCCGCGCTGGCAAAGCTGGAAAGCCTTGATGAAGTCACTGAAGCCTATTACACAACCGGCCAc > 1:3385814/1‑69 (MQ=255) tACCCTTCCGCGCTGGCAAAGCTGGAAAGCCTTGATGAAGTCACTGAAGCCTATTACACAACCGGCCAc > 1:2907633/1‑69 (MQ=255) tACCCTTCCGCGCTGGCAAAGCTGGAAAGCCTTGATGAAGTCACTGAAGCCTATTACACAACCGGCCAc > 1:2835759/1‑69 (MQ=255) tACCCTTCCGCGCTGGCAAAGCTGGAAAGCCTTGATGAAGTCACTGAAGCCTATTACACAACCGGCCAc > 1:2389292/1‑69 (MQ=255) tACCCTTCCGCGCTGGCAAAGCTGGAAAGCCTTGATGAAGTCACTGAAGCCTATTACACAACCGGCCAc > 1:2078541/1‑69 (MQ=255) tACCCTTCCGCGCTGGCAAAGCTGGAAAGCCTTGATGAAGTCACTGAAGCCTATTACACAACCGGCCAc > 1:920158/1‑69 (MQ=255) ttCCGCGCTGGCAAAGCTGGAAAGCCTTGATGAAGTCACTGAAGCCTATTACACAACCGGCCACTa > 1:673106/1‑66 (MQ=255) ttCCGCGCTGGCAAAGCTGGAAAGCCTTGATGAAGTCACTGAAGCCTATTACACAACCGGCCACTa > 1:2265599/1‑66 (MQ=255) ttCCGCGCTGGCAAAGCTGGAAAGCCTTGATGAAGTCACTGAAGCCTATTACACAACCGGCCACTa > 1:880327/1‑66 (MQ=255) gcTGGCAAAGCTGGAAAGCCTTGATGAAGTCACTGAAGCCTATTACACAACCGGCCACTACAGCATCtt < 1:1307628/69‑1 (MQ=255) | TCGGTTATGACGTAGGCTGCTTTATCGGCATTATATTAAAGAGCGCCAAAGACTACCCTTCCGCGCTGGCAAAGCTGGAAAGCCTTGATGAAGTCACTGAAGCCTATTACACAACCGGCCACTACAGCATCTT > minE/2459581‑2459713 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |