| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,459,805 | T→C | 45.0% | L139L (TTG→CTG) | asnC → | DNA‑binding transcriptional dual regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,459,805 | 0 | T | C | 45.0% | 18.1 / 91.1 | 110 | L139L (TTG→CTG) | asnC | DNA‑binding transcriptional dual regulator |
| Reads supporting (aligned to +/- strand): ref base T (21/39); new base C (35/14); total (57/53) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.25e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 110 total aligned reads displayed.
ACGCTCTCCAGCATGTACTTATCAACAAGATCCAAACAATTGATGAAATTCAGTCCACCGAGACATTGATCGTCCTGCAAAACCCGATCATGCGTACCATCAAGCCCTGATCGGCTTTTTTAATCCCA > minE/2459740‑2459867 | atgCTCTCTAGCATGTACTTATCAACAAGATCCAAACAATTGATGAAATTCAGTCCACCGAGACACTGa > 1:3139313/3‑69 (MQ=255)atgCTCTCCAGCATGTACTTATCAACAAGATCCAAACAATTGATGAAATTCAGTCCACCGAGACACTg > 1:3028106/3‑68 (MQ=255)atgCTCTCCAGCATGTACTTATCAACAAGATCCAAACAATTGATGAAATTCAGTCCACCGAGACACTGa > 1:1969348/3‑69 (MQ=255)atgCTCTCCAGCATGTACTTATCAACAAGATCCAAACAATTGATGAAATTCAGTCCACCGAGACACTGa > 1:2567415/3‑69 (MQ=255)atgCTCTCCAGCATGTACTTATCAACAAGATCCAAACAATTGATGAAATTCAGTCCACCGAGACACTGa > 1:2071754/3‑69 (MQ=255)atgCTCTCCAGCATGTACTTATCAACAAGATCCAAACAATTGATGAAATTCAGTCCACCGAGACACTGa > 1:213100/3‑69 (MQ=255)atgCTCTCCAGCATGTACTTATCAACAAGATCCAAACAATTGATGAAATTCAGTCCACCGAGACACTGa > 1:2150802/3‑69 (MQ=255)atgCTCTCCAGCATGTACTTATCAACAAGATCCAAACAATTGATGAAATTCAGTCCACCGAGACACTGa > 1:2521574/3‑69 (MQ=255)atgCTCTCCAGCATGTACTTATCAACAAGATCCAAACAATTGATGAAATTCAGTCCACCGAGACACTGa > 1:1008108/3‑69 (MQ=255)atgCTCTCCAGCATGTACTTATCAACAAGATCCAAACAATTGATGAAATTCAGTCCACCGAGACACTGa > 1:2568149/3‑69 (MQ=255)atgCTCTCCAGCATGTACTTATCAACAAGATCCAAACAATTGATGAAATTCAGTCCACCGAGACACTGa > 1:2605412/3‑69 (MQ=255)atgCTCTCCAGCATGTACTTATCAACAAGATCCAAACAATTGATGAAATTCAGTCCACCGAGACACTGa > 1:2641915/3‑69 (MQ=255)atgCTCTCCAGCATGTACTTATCAACAAGATCCAAACAATTGATGAAATTCAGTCCACCGAGACACTGa > 1:2721869/3‑69 (MQ=255)atgCTCTCCAGCATGTACTTATCAACAAGATCCAAACAATTGATGAAATTCAGTCCACCGAGACACTGa > 1:2885493/3‑69 (MQ=255)atgCTCTCCAGCATGTACTTATCAACAAGATCCAAACAATTGATGAAATTCAGTCCACCGAGACACTGa > 1:3409979/3‑69 (MQ=255)atgCTCTCCAGCATGTACTTATCAACAAGATCCAAACAATTGATGAAATTCAGTCCACCGAGACACTGa > 1:3424661/3‑69 (MQ=255)atgCTCTCCAGCATGTACTTATCAACAAGATCCAAACAATTGATGAAATTCAGTCCACCGAGACACTGa > 1:435239/3‑69 (MQ=255)atgCTCTCCAGCATGTACTTATCAACAAGATCCAAACAATTGATGAAATTCAGTCCACCGAGACACTGa > 1:491692/3‑69 (MQ=255)atgCTCTCCAGCATGTACTTATCAACAAGATCCAAACAATTGATGAAATTCAGTCCACCGAGACACTGa > 1:565616/3‑69 (MQ=255)atgCTCTCCAGCATGTACTTATCAACAAGATCCAAACAATTGATGAAATTCAGTCCACCGAGACACTGa > 1:63847/3‑69 (MQ=255)atgCTCTCCAGCATGTACTTATCAACAAGATCCAAACAATTGATGAAATTCAGTCCACCGAGACACTGa > 1:670277/3‑69 (MQ=255)atgCTCTCCAGCATGTACTTATCAACAAGATCCAAACAATTGATGAAATTCAGTCCACCGAGACACTGa > 1:698104/3‑69 (MQ=255)atgCTCTCCAGCATGTACTTATCAACAAGATCCAAACAATTGATGAAATTCAGTCCACCGAGACACTGa > 1:728779/3‑69 (MQ=255)atgCTCTCCAGCATGTACTTATCAACAAGATCCAAACAATTGATGAAATTCAGTCCACCGAGACACTGa > 1:947690/3‑69 (MQ=255)atgCTCTCCAGCATGTACTTATCAACAAGATCCAAACAATTGATGAAATTCAGTCCACCGAGACACTGa > 1:995422/3‑69 (MQ=255)atgCTCTCCAGCATGTACTTATCAACAAGATCCAAACAATTGATGAAATTCAGTCCACCGAGACACTGa > 1:1919268/3‑69 (MQ=255)atgCTCTCCAGCATGTACTTATCAACAAGATCCAAACAATTGATGAAATTCAGTCCACCGAGACACTGa > 1:1673212/3‑69 (MQ=255)atgCTCTCCAGCATGTACTTATCAACAAGATCCAAACAATTGATGAAATTCAGTCCACCGAGACACTGa > 1:125977/3‑69 (MQ=255)atgCTCTCAAGCATGTACTTATCAACAAGATCCAAACAATTGATGAAATTCAGTCCACCGAGACACTGa > 1:1304179/3‑69 (MQ=255) gCATGTACTTATCAACAAGATCCAAACAATTGATGAAATTCAGTCCACCGAGACACTGATCGTCCTGc < 1:2131359/68‑1 (MQ=255) gCATGTACTTATCAACAAGATCCAAACAATTGATGAAATTCAGTCCACCGAGACACTGATCGTCCTGc < 1:2661010/68‑1 (MQ=255) gCATGTACTTATCAACAAGATCCAAACAATTGATGAAATTCAGTCCACCGAGACACTGATCGTCCTGc < 1:3408064/68‑1 (MQ=255) gCATGTACTTATCAACAAGATCCAAACAATTGATGAAATTCAGTCCACCGAGACACTGATCGTCCTGc < 1:453090/68‑1 (MQ=255) gCATGTACTTATCAACAAGATCCAAACAATTGATGAAATTCAGTCCACCGAGACACTGATCGTCCTGc < 1:1219061/68‑1 (MQ=255) tACTTATCAACAAGATCCAAACAATTGATGAAATTCAGTCCACCGAGACATTGATCGTCCTGCaaa > 1:846236/1‑66 (MQ=255) tACTTATCAACAAGATCCAAACAATTGATGAAATTCAGTCCACCGAGACATTGATCGTCCTGCAAAAc > 1:541394/1‑68 (MQ=255) tACTTATCAACAAGATCCAAACAATTGATGAAATTCAGTCCACCGAGACATTGATCGTCCTGCAAAAc > 1:465916/1‑68 (MQ=255) tACTTATCAACAAGATCCAAACAATTGATGAAATTCAGTCCACCGAGACATTGATCGTCCTGCAAAAc > 1:1918691/1‑68 (MQ=255) tACTTATCAACAAGATCCAAACAATTGATGAAATTCAGTCCACCGAGACATTGATCGTCCTGCAAAAc > 1:1081577/1‑68 (MQ=255) tACTTATCAACAAGATCCAAACAATTGATGAAATTCAGTCCACCGAGACATTGATCGTCCTGCAAAAc > 1:105792/1‑68 (MQ=255) tACTTATCAACAAGATCCAAACAATTGATGAAATTCAGTCCACCGAGACATTGATCGTCCTGCAAAAc > 1:3201765/1‑68 (MQ=255) tACTTATCAACAAGATCCAAACAATTGATGAAATTCAGTCCACCGAGACATTGATCGTCCTGCAAAAc > 1:2780664/1‑68 (MQ=255) tACTTATCAACAAGATCCAAACAATTGATGAAATTCAGTCCACCGAGACATTGATCGTCCTGCAAAAc > 1:1049525/1‑68 (MQ=255) tACTTATCAACAAGATCCAAACAATTGATGAAATTCAGTCCACCGAGACATTGATCGTCCTGCAAAAc > 1:1466066/1‑68 (MQ=255) tACTTATCAACAAGATCCAAACAATTGATGAAATTCAGTCCACCGAGACATTGATCGTCCTGCAAAAc > 1:1026843/1‑68 (MQ=255) tACTTATCAACAAGATCCAAACAATTGATGAAATTCAGTCCACCGAGACATTGATCGTCCTGCAAAAc > 1:2488476/1‑68 (MQ=255) tACTTATCAACAAGATCCAAACAATTGATGAAATTCAGTCCACCGAGACATTGATCGTCCTGCAAAAc > 1:2318443/1‑68 (MQ=255) tACTTATCAACAAGATCCAAACAATTGATGAAATTCAGTCCACCGAGACATTGATCGTCCTGCAAAAc > 1:2201924/1‑68 (MQ=255) tACTTATCAACAAGATCCAAACAATTGATGAAATTCAGTCCACCGAGACATTGATCGTCCTGCAAAAc > 1:2034157/1‑68 (MQ=255) tACTTATCAACAAGATCCAAACAATTGATGAAATTCAGTCCACCGAGACATTGATCGTCCTGCAAAAc > 1:2115929/1‑68 (MQ=255) tACTTATCAACAAGATCCAAACAATTGATGAAATTCAGTCCACCGAGACATTGATCGTCCTGCAAAAc > 1:2289717/1‑68 (MQ=255) tACTTATCAACAAGATCCAAACAATTGATGAAATTCAGTACACCGAGACATTGATCGTCCTGCAAAAc > 1:944057/1‑68 (MQ=255) tACTTATCAACAAGATCCAAACAATTGATGAAAATCAGTCCACCGAGACATAGAt > 1:2585802/1‑55 (MQ=255) tACTAATCAACAAGATCCAAACAATTGATGAAATTCAGTCCACCGAGACAATGATCGTCCTGCaaaa > 1:2407661/1‑67 (MQ=255) tATCAACAAGATCCAAACAATTGATGAAATTCAGTCCACCGAGACATTGATCGTCCTGCAAAACCCGa < 1:2289238/68‑1 (MQ=255) tATCAACAAGATCCAAACAATTGATGAAATTCAGTCCACCGAGACATTGATCGTCCTGCAAAACCCGAt < 1:203391/69‑1 (MQ=255) tATCAACAAGATCCAAACAATTGATGAAATTCAGTCCACCGAGACATTGATCGTCCTGCAAAACCCGAt < 1:1710701/69‑1 (MQ=255) tATCAACAAGATCCAAACAATTGATGAAATTCAGTCCACCGAGACATTGATCGTCCTGCAAAACCCGAt < 1:388166/69‑1 (MQ=255) tATCAACAAGATCCAAACAATTGATGAAATTCAGTCCACCGAGACATTGATCGTCCTGCAAAACCCGAt < 1:1075122/69‑1 (MQ=255) tATCAACAAGATCCAAACAATTGATGAAATTCAGTCCACCGAGACATTGATAGTCCTGCAAAACCCGAt < 1:3356268/69‑1 (MQ=255) aacaaGATCCAAACAATTGATGAAATTCAGTCCACCGAGACATTGATCGTCCTGCAAAACCCGATCATg < 1:2763545/69‑1 (MQ=255) aacaaGATCCAAACAATTGATGAAATTCAGTCCACCGAGACATTGATCGTCCTGCAAAACCCGATCATg < 1:3054075/69‑1 (MQ=255) aacaaGATCCAAACAATTGATGAAATTCAGTCCACCGAGACATTGATCGTCCTGCAAAACCCGATCATg < 1:1268238/69‑1 (MQ=255) caaGATCCAAACAATTGATGAAATTCAGTCCACCGAGACATTGATCGTCCTGCAAAACCCGAt > 1:2614924/1‑63 (MQ=255) aTCCAAACGATTGATGAAATTCAGTCCACCGAGACATTGATCGTCCTGCAAAACCCGATCATGCGTAcc < 1:839994/69‑1 (MQ=255) aTCCAAACAATTGATTAAATTCAGTCCACCGAGACATTGATCGTCCTGCAAAACCCGATCATGCGTAcc < 1:1244697/69‑1 (MQ=255) aTCCAAACAATTGATGAAATTCAGTCCACCGATACATTGATCGTCCTGCAAAACCCGATCATGCGTAcc < 1:1548229/69‑1 (MQ=255) aTCCAAACAATTGATGAAATTCAGTCCACCGAGACATTGATCGTCCTGCAAAACCCGATCATGCGTAcc < 1:1907802/69‑1 (MQ=255) aTCCAAACAATTGATGAAATTCAGTCCACCGAGACATTGATCGTCCTGCAAAACCCGATCATGCGTAcc < 1:638577/69‑1 (MQ=255) aTCCAAACAATTGATGAAATTCAGTCCACCGAGACATTGATCGTCCTGCAAAACCCGATCATGCGTAcc < 1:1219108/69‑1 (MQ=255) aTCCAAACAATTGATGAAATTCAGTCCACCGAGACATTGATCGTCCTGCAAAACCCGATCATGCGTAcc < 1:935865/69‑1 (MQ=255) aTCCAAACAATTGATGAAATTCAGTCCACCGAGACATTGATCGTCCTGCAAAACCCGATCATGCGTAcc < 1:238313/69‑1 (MQ=255) aTCCAAACAATTGATGAAATTCAGTCCACCGAGACATTGATCGTCCTGCAAAACCCGATCATGCGTAcc < 1:2194119/69‑1 (MQ=255) aTCCAAACAATTGATGAAATTCAGTCCACCGAGACATTGATCGTCCTGCAAAACCCGATCATGCGTAcc < 1:2818550/69‑1 (MQ=255) aTCCAAACAATTGATGAAATTCAGTCCACCGAGACATTGATCGTCCTGCAAAACCCGATCATGCGTAcc < 1:134928/69‑1 (MQ=255) aTCCAAACAATTGATGAAATTCAGTCCACCGAGACATTGATCGTCCTGCAAAACCCGATCATGCGTAcc < 1:2920076/69‑1 (MQ=255) tCCAAACAATTGATGAAATTCAGTCCACCGAGACACTGATCGTCCTGCAGAATCCGATCATGCGTACCa < 1:3238179/69‑1 (MQ=255) tCCAAACAATTGATGAAATTCAGTCCACCGAGACACTGATCGTCCTGCAGAATCCGATCATGCGTACCa < 1:2032471/69‑1 (MQ=255) tCCAAACAATTGATGAAATTCAGTCCACCGAGACACTGATCGTCCTGCAGAATCCGATCATGCGTACCa < 1:2585449/69‑1 (MQ=255) tCCAAACAATTGATGAAATTCAGTCCACCGAGACACTGATCGTCCTGCAGAATCCGATCATGCGTACCa < 1:2640753/69‑1 (MQ=255) tCCAAACAATTGATGAAATTCAGTCCACCGAGACACTGATCGTCCTGCAGAATCCGATCATGCGTACCa < 1:730684/69‑1 (MQ=255) tCCAAACAATTGATGAAATTCAGTCCACCGAGACACTGATCGTCCTGCAGAATCCGATCATGCGTACCa < 1:2123896/69‑1 (MQ=255) tCCAAACAATTGATGAAATTCAGTCCACCGAGACACTGATCGTCCTGCAGAATCCGATCATGCGTACCa < 1:2147861/69‑1 (MQ=255) tCCAAACAATTGATGAAATTCAGTCCACCGAGACACTGATCGTCCTGCAGAATCCGATCATGCGTACCa < 1:1287994/69‑1 (MQ=255) ccAAACAATTGATGAAATTCAGTCCACCGAGACACTGATCGTCCTGCAGAATCCGATCATGCGTACCa < 1:8924/68‑1 (MQ=255) aCAATTGATGAAATTCAGTCCACCGAGACATTGATCGTCCTGCAAAACCCGATCATGCGTACCATCAAg < 1:1833521/69‑1 (MQ=255) aCAATTGATGAAATTCAGTCCACCGAGACATTGATCGTCCTGCAAAACCCGATCATGCGTACCATCAAg < 1:3301960/69‑1 (MQ=255) aCAATTGATGAAATTCAGTCCACCGAGACATTGATCGTCCTGCAAAACCCGATCATGCGTACCATCAAg < 1:387597/69‑1 (MQ=255) aCAATTGATGAAATTCAGTCCACCGAGACATTGATCGTCCTGCAAAACCCGATCATGCGTACCATCAAg < 1:2269808/69‑1 (MQ=255) cAATTGATGAAATTCAGTCCACCGAGACATTGATCGTCCTGCAAAACCCGAt < 1:1152210/52‑1 (MQ=255) tgatgaAATTCAGTCCACCGAGACATTGATCGTCCTGCAAAACCCGATCATGCGTACCATCAAGCCCTg < 1:2280546/69‑1 (MQ=255) tgatgaAATTCAGTCCACCGAGACATTGATCGTCCTGCAAAACCCGATCATGCGTACCATCAAGCCCTg < 1:1377026/69‑1 (MQ=255) tgatgaAATTCAGTCCACCGAGACATTGATCGTCCTGCAAAACCCGATCATGCGTACCATCAAGCCCTg < 1:1640896/69‑1 (MQ=255) tgatgaAATTCAGTCCACCGAGACATTGATCGTCCTGCAAAACCCGATCATGCGTACCATCAAGCCCTg < 1:2346708/69‑1 (MQ=255) gaAATTCAGTCCACCGAGACATTGATCGTCCTGCAAAACCCGATCATGCGTACCATCAAGCCCTGATCg < 1:557331/69‑1 (MQ=255) gaAATTCAGTCCACCGAGACATTGATCGTCCTGCAAAACCCGATCATGCGTACCATCAAGCCCTGATCg < 1:940259/69‑1 (MQ=255) gaAATTCAGTCCACCGAGACATTGATCGTCCTGCAAAACCCGATCATGCGTACCATCAAGCCCTGATCg < 1:1663855/69‑1 (MQ=255) gagaCATTGATCGTCCTGCAAAACCCGATCATGCGTACCATCAAGCCCTGATCGGCTTTTTTAATCCCa < 1:2110803/69‑1 (MQ=255) gagaCATTGATCGTCCTGCAAAACCCGATCATGCGTAACATCAAGCCCTGATCGGCTTTTTTAATCCCa < 1:485071/69‑1 (MQ=255) agaCATTGATCGTCCTGCAAAACCCGATCATGCGTACCATCAAGCCCTg < 1:3131205/49‑1 (MQ=255) | ACGCTCTCCAGCATGTACTTATCAACAAGATCCAAACAATTGATGAAATTCAGTCCACCGAGACATTGATCGTCCTGCAAAACCCGATCATGCGTACCATCAAGCCCTGATCGGCTTTTTTAATCCCA > minE/2459740‑2459867 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |